BBa_I756027 1 BBa_I756027 LexAopMLP-GFP-SV40PA 2007-10-24T11:00:00Z 2015-08-31T04:08:07Z Mfe1, ClaI (1-12bp) LexAopMLP (minimal promoter) from pSH1834 plasmid (13-219bp) Asc1 (220-227bp) Kozak box (228-233bp) GFP from PWPI lentiviral plasmid (235-981bp) STOP STOP (983-987bp) Xba1 (988-993bp) Pst1 (994-999) SV40PA from pcDNA3.1 zeo (+) plasmid (1000-1130bp) HIndIII (1131-1136bp) Reporter construct for signaling circuit. GFP under the control of the lexAopMLP promoter and a SV40 polyA tail. SCFV-Epor-gp130, FcGamma, Syk, P85, CD11a, CD18-Cub, and CD18-nubG all have output of lexA-VP16 which will bind the LexAop and turn on the expression of GFP. false false _108_ 0 1828 9 Not in stock false LexAop is a repeated sequence which results in multiple products. Isolate the largest fragment for gel isolation false Jennifer Lei BBa_I756027_sequence 1 caattgatcgattcgactgctgtatataaaaccagtggttatatgtacagtactgctgtatataaaaccagtggttatatgtacagtacgtcgactgctgtatataaaaccagtggttatatgtacagtactgctgtatataaaaccagtggttatatgtacagtacgcgtgaccgggtgttcctgaaggggggctataaaagggggtgggggcgcgttggcgcgccgccaccaatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccctgacctacggcgtgcagtgcttcagccgctaccccgaccacatgaagcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagcacccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagtccggactcagatctcgactagctagtagctagtaatctagactgcagaacttgtttattgcagcttataatggttacaaataaagcaatagcatcacaaatttcacaaataaagcatttttttcactgcattctagttgtggtttgtccaaactcatcaatgtatcttatcatgtctgaagctt igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z