BBa_I756029 1 BBa_I756029 Syk-dnaEc-VP16AD 2007-10-24T11:00:00Z 2015-08-31T04:08:07Z Syk from OpenBiosystems (Clone ID: 30533300) dnaEc from dnaE gene of Synechocystis sp.(split inteins)(2624-2749bp) VP16AD from HSV1 helper virus (Sapolsky Lab, Stanford)(2750-2887bp) Syk kinase with SH2 domains coupled at the C terminus to dnaEc-VP16(activation domain). This construct was inserted into pcDNA3.1(+)Zeo using XbaI and EcoRV. false false _108_ 0 2113 9 Not in stock false A flexible GS Linker (GGGGS)x5 (or more) is required between the dnaEc and Syk. This was quite difficult to clone in with the FseI site used in the part. false Omar Khan BBa_I756029_sequence 1 atggcgggaagtgctgtggacagcgccaaccacctgacctacctttttggcaacatcacccgggaagaggctgaagactacctggtccagggaggcatgaccgatgggctctacctgctacgccagagccgcaattacctgggtggttttgctttgtcggtggctcacaacaggaaggcacaccactacaccatcgagagggaacttaatggcacctacgccatctccgggggcagggcccatgccagcccagcagacctctgccattaccactcccaggaacctgatggccttatctgcctccttaagaagcccttcaaccggcccccgggagtacagcccaagaccggaccctttgaggacctgaaggagaacctcatcagggaatatgtgaaacagacctggaaccttcagggccaggctctggagcaagccatcatcagccagaagccccagctggagaagctgatcgccaccacggcccatgagaagatgccctggttccatggcaacatctccagagatgaatcagagcagacggtcctcatagggtcaaagaccaatggaaaattcctgatcagggccagagacaacagcggctcctatgctctgtgcctgctgcacgaagggaaagtattgcactaccgcattgacagggacaagaccgggaagctctccattcctgaggggaagaagtttgacaccctctggcagctagtggaacattactcttacaagccagatgggctactaagagtcctcacggtaccatgccaaaagattggtgcacagatgggccacccaggaagcccaaatgcccatcccgtgacttggtcaccgggtggaataatctcaaggatcaaatcctactccttcccaaagcctggccacaaaaagcctgccccaccccaagggagccgtccagagagcactgtgtccttcaacccctatgagccaacgggagggccctggggcccagacagaggccttcagagagaagccctgcccatggacacagaggtgtacgagagcccttatgctgaccctgaagagatccggcccaaagaggtctacctggacaggagcctgctgaccctggaggacaatgaactgggctccggtaacttcgggactgtgaaaaagggatactaccaaatgaaaaaagttgtgaaaaccgtggctgtgaaaatcctgaagaacgaggccaacgacccggctttgaaggacgagctgctggcagaggcgaacgtcatgcagcagctggacaacccctacattgtgcgcatgatcggaatctgcgaggcggagtcctggatgctggtgatggagatggcggagctggggccgctcaacaagtacctgcagcagaacaggcacattaaggataagaacatcatagagctggttcaccaggtttccatggggatgaagtatttggaagagagcaactttgtgcacagagatctggctgcgcggaacgtgcttctggtcacacagcactatgccaagatcagcgatttcggtctttccaaagccctgcgtgctgatgaaaactactacaaggcccagacccacgggaagtggcccgtgaagtggtacgcccccgaatgcatcaactactacaagttctccagtaagagtgacgtctggagcttcggagtcctgatgtgggaagcgttctcctatgggcagaagccctacagagggatgaaagggagcgaagtgaccgccatgctggagaaaggagagcggatggggtgccctgcaggatgcccgagagagatgtacgacctgatgaacctgtgctggacttacgatgtggagaacaggccaggattcacagctgtggaactgaggcttcgcaattactactacgacgtggttaactaaggccggccatggttaaagttatcggtcgtcgttccctcggagtgcaaagaatatttgatattggtcttccccaagaccataattttctgctagccaatggggcgatcgccgccaattgtttcaacaagtcacactcgacggcccccccgaccgatgtcagcctgggggacgagctccacttagacggcgaggacgtggcgatggcgcatgccgacgcgctagacgatttcgatctggacatgttgggggacggggattccccgggtccgggatctaga igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z