LOCUS       BBa_I758005              930 bp    DNA     linear       30-Dec-2025
DEFINITION  Prm->tetR (term+)
ACCESSION   BBa_I758005
VERSION     BBa_I758005.1  
FEATURES             Location/Qualifiers
     misc_feature    9..25
                     /label=OR1
     misc_feature    33..49
                     /label=OR2
     promoter        48..53
                     /label=-35
     misc_feature    59..65
                     /label=mutate to obliterate OR3
     promoter        71..76
                     /label=-10
     promoter        1..82
                     /label=BBa_I12007
     misc_feature    95..98
                     /label=conserved
     RBS             91..102
                     /label=BBa_B0034
     CDS             112..728
                     /label=tetR
     misc_feature    729..762
                     /label=SsrA
     misc_feature    769..793
                     /label=Help:Barcodes
     CDS             109..768
                     /label=BBa_C0040
     stem_loop       813..856
                     /label=stem_loop
     misc_feature    802..881
                     /label=BBa_B0010
     terminator      802..881
                     /label=BBa_B0010
     stem_loop       897..916
                     /label=T7 TE
     misc_feature    923..923
                     /label=stop
     polyA_site      917..930
                     /label=polya
     misc_feature    890..930
                     /label=BBa_B0012
     terminator      890..930
                     /label=BBa_B0012
     misc_feature    91..930
                     /label=BBa_P0440
ORIGIN
        1 gcaaccatta tcaccgccag aggtaaaata gtcaacacgc acggtgttag atatttataa
       61 atagtggtga tagatttaac gttactagag aaagaggaga aatactagat gtccagatta
      121 gataaaagta aagtgattaa cagcgcatta gagctgctta atgaggtcgg aatcgaaggt
      181 ttaacaaccc gtaaactcgc ccagaagcta ggtgtagagc agcctacatt gtattggcat
      241 gtaaaaaata agcgggcttt gctcgacgcc ttagccattg agatgttaga taggcaccat
      301 actcactttt gccctttaga aggggaaagc tggcaagatt ttttacgtaa taacgctaaa
      361 agttttagat gtgctttact aagtcatcgc gatggagcaa aagtacattt aggtacacgg
      421 cctacagaaa aacagtatga aactctcgaa aatcaattag cctttttatg ccaacaaggt
      481 ttttcactag agaatgcatt atatgcactc agcgctgtgg ggcattttac tttaggttgc
      541 gtattggaag atcaagagca tcaagtcgct aaagaagaaa gggaaacacc tactactgat
      601 agtatgccgc cattattacg acaagctatc gaattatttg atcaccaagg tgcagagcca
      661 gccttcttat tcggccttga attgatcata tgcggattag aaaaacaact taaatgtgaa
      721 agtgggtccg ctgcaaacga cgaaaactac gctttagtag cttaataaca ctgatagtgc
      781 tagtgtagat cactactaga gccaggcatc aaataaaacg aaaggctcag tcgaaagact
      841 gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct ctactagagt cacactggct
      901 caccttcggg tgggcctttc tgcgtttata
//