LOCUS BBa_I758009 3799 bp DNA linear 21-Jan-2026 DEFINITION Screen for testing electric field-activated mutant tf's in liquid ACCESSION BBa_I758009 VERSION BBa_I758009.1 FEATURES Location/Qualifiers misc_feature 3..19 /label=lac O1 promoter 20..25 /label=-35 misc_feature 26..42 /label=lac O1 promoter 43..48 /label=-10 misc_feature 1..54 /label=BBa_R0011 promoter 1..54 /label=BBa_R0011 misc_feature 68..71 /label=conserved RBS 64..75 /label=BBa_B0034 CDS 85..792 /label=cI lambda misc_feature 793..825 /label=LVA misc_feature 832..856 /label=Help:Barcodes CDS 82..856 /label=BBa_C0051 stem_loop 872..891 /label=T7 TE misc_feature 898..898 /label=stop polyA_site 892..905 /label=polya misc_feature 865..905 /label=BBa_B0012 terminator 865..905 /label=BBa_B0012 stem_loop 926..948 /label=stem_loop misc_feature 914..959 /label=BBa_B0011 terminator 914..959 /label=BBa_B0011 misc_feature 1..959 /label=BBa_I5010 promoter 1005..1010 /label=-10 promoter 982..987 /label=-35 misc_feature 992..1008 /label=OR1 misc_feature 968..984 /label=OR2 misc_feature 968..1016 /label=BBa_R0051 promoter 968..1016 /label=BBa_R0051 misc_feature 1029..1032 /label=conserved RBS 1025..1036 /label=BBa_B0034 CDS 1043..1765 /label=BBa_E0030 stem_loop 1785..1828 /label=stem_loop misc_feature 1774..1853 /label=BBa_B0010 terminator 1774..1853 /label=BBa_B0010 stem_loop 1869..1888 /label=T7 TE misc_feature 1895..1895 /label=stop polyA_site 1889..1902 /label=polya misc_feature 1862..1902 /label=BBa_B0012 terminator 1862..1902 /label=BBa_B0012 misc_feature 968..1902 /label=BBa_I6036 misc_feature 1..1902 /label=BBa_S03817 misc_feature 1919..1935 /label=OR1 misc_feature 1943..1959 /label=OR2 promoter 1958..1963 /label=-35 misc_feature 1969..1975 /label=mutate to obliterate OR3 promoter 1981..1986 /label=-10 promoter 1911..1992 /label=BBa_I12007 misc_feature 1..1992 /label=BBa_S03818 misc_feature 2005..2008 /label=conserved RBS 2001..2012 /label=BBa_B0034 CDS 2019..2693 /label=mrfp1 misc_feature 2700..2724 /label=Help:Barcodes CDS 2019..2699 /label=BBa_E1010 stem_loop 2744..2787 /label=stem_loop misc_feature 2733..2812 /label=BBa_B0010 terminator 2733..2812 /label=BBa_B0010 stem_loop 2828..2847 /label=T7 TE misc_feature 2854..2854 /label=stop polyA_site 2848..2861 /label=polya misc_feature 2821..2861 /label=BBa_B0012 terminator 2821..2861 /label=BBa_B0012 misc_feature 2001..2861 /label=BBa_I13507 misc_feature 1..2861 /label=BBa_S03819 misc_feature 2878..2894 /label=OR1 misc_feature 2902..2918 /label=OR2 promoter 2917..2922 /label=-35 misc_feature 2928..2934 /label=mutate to obliterate OR3 promoter 2940..2945 /label=-10 promoter 2870..2951 /label=BBa_I12007 misc_feature 2964..2967 /label=conserved RBS 2960..2971 /label=BBa_B0034 CDS 2981..3597 /label=tetR misc_feature 3598..3631 /label=SsrA misc_feature 3638..3662 /label=Help:Barcodes CDS 2978..3637 /label=BBa_C0040 stem_loop 3682..3725 /label=stem_loop misc_feature 3671..3750 /label=BBa_B0010 terminator 3671..3750 /label=BBa_B0010 stem_loop 3766..3785 /label=T7 TE misc_feature 3792..3792 /label=stop polyA_site 3786..3799 /label=polya misc_feature 3759..3799 /label=BBa_B0012 terminator 3759..3799 /label=BBa_B0012 misc_feature 2960..3799 /label=BBa_P0440 misc_feature 2870..3799 /label=BBa_I758005 ORIGIN 1 aattgtgagc ggataacaat tgacattgtg agcggataac aagatactga gcacatacta 61 gagaaagagg agaaatacta gatgagcaca aaaaagaaac cattaacaca agagcagctt 121 gaggacgcac gtcgccttaa agcaatttat gaaaaaaaga aaaatgaact tggcttatcc 181 caggaatctg tcgcagacaa gatggggatg gggcagtcag gcgttggtgc tttatttaat 241 ggcatcaatg cattaaatgc ttataacgcc gcattgcttg caaaaattct caaagttagc 301 gttgaagaat ttagcccttc aatcgccaga gaaatctacg agatgtatga agcggttagt 361 atgcagccgt cacttagaag tgagtatgag taccctgttt tttctcatgt tcaggcaggg 421 atgttctcac ctgagcttag aacctttacc aaaggtgatg cggagagatg ggtaagcaca 481 accaaaaaag ccagtgattc tgcattctgg cttgaggttg aaggtaattc catgaccgca 541 ccaacaggct ccaagccaag ctttcctgac ggaatgttaa ttctcgttga ccctgagcag 601 gctgttgagc caggtgattt ctgcatagcc agacttgggg gtgatgagtt taccttcaag 661 aaactgatca gggatagcgg tcaggtgttt ttacaaccac taaacccaca gtacccaatg 721 atcccatgca atgagagttg ttccgttgtg gggaaagtta tcgctagtca gtggcctgaa 781 gagacgtttg gcgctgcaaa cgacgaaaac tacgctttag tagcttaata acgctgatag 841 tgctagtgta gatcgctact agagtcacac tggctcacct tcgggtgggc ctttctgcgt 901 ttatatacta gagagagaat ataaaaagcc agattattaa tccggctttt ttattatttt 961 actagagtaa caccgtgcgt gttgactatt ttacctctgg cggtgataat ggttgctact 1021 agagaaagag gagaaatact agatggtgag caagggcgag gagctgttca ccggggtggt 1081 gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga 1141 gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa 1201 gctgcccgtg ccctggccca ccctcgtgac caccttcggc tacggcctgc aatgcttcgc 1261 ccgctacccc gaccacatga agctgcacga cttcttcaag tccgccatgc ccgaaggcta 1321 cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt 1381 gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga 1441 ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat 1501 catggccgac aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga 1561 ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc 1621 cgtgctgctg cccgacaacc actacctgag ctaccagtcc gccctgagca aagaccccaa 1681 cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg 1741 catggacgag ctgtacaagt aataatacta gagccaggca tcaaataaaa cgaaaggctc 1801 agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctctactaga 1861 gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta tatactagag gcaaccatta 1921 tcaccgccag aggtaaaata gtcaacacgc acggtgttag atatttataa atagtggtga 1981 tagatttaac gttactagag aaagaggaga aatactagat ggcttcctcc gaagacgtta 2041 tcaaagagtt catgcgtttc aaagttcgta tggaaggttc cgttaacggt cacgagttcg 2101 aaatcgaagg tgaaggtgaa ggtcgtccgt acgaaggtac ccagaccgct aaactgaaag 2161 ttaccaaagg tggtccgctg ccgttcgctt gggacatcct gtccccgcag ttccagtacg 2221 gttccaaagc ttacgttaaa cacccggctg acatcccgga ctacctgaaa ctgtccttcc 2281 cggaaggttt caaatgggaa cgtgttatga acttcgaaga cggtggtgtt gttaccgtta 2341 cccaggactc ctccctgcaa gacggtgagt tcatctacaa agttaaactg cgtggtacca 2401 acttcccgtc cgacggtccg gttatgcaga aaaaaaccat gggttgggaa gcttccaccg 2461 aacgtatgta cccggaagac ggtgctctga aaggtgaaat caaaatgcgt ctgaaactga 2521 aagacggtgg tcactacgac gctgaagtta aaaccaccta catggctaaa aaaccggttc 2581 agctgccggg tgcttacaaa accgacatca aactggacat cacctcccac aacgaagact 2641 acaccatcgt tgaacagtac gaacgtgctg aaggtcgtca ctccaccggt gcttaataac 2701 gctgatagtg ctagtgtaga tcgctactag agccaggcat caaataaaac gaaaggctca 2761 gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tctactagag 2821 tcacactggc tcaccttcgg gtgggccttt ctgcgtttat atactagagg caaccattat 2881 caccgccaga ggtaaaatag tcaacacgca cggtgttaga tatttataaa tagtggtgat 2941 agatttaacg ttactagaga aagaggagaa atactagatg tccagattag ataaaagtaa 3001 agtgattaac agcgcattag agctgcttaa tgaggtcgga atcgaaggtt taacaacccg 3061 taaactcgcc cagaagctag gtgtagagca gcctacattg tattggcatg taaaaaataa 3121 gcgggctttg ctcgacgcct tagccattga gatgttagat aggcaccata ctcacttttg 3181 ccctttagaa ggggaaagct ggcaagattt tttacgtaat aacgctaaaa gttttagatg 3241 tgctttacta agtcatcgcg atggagcaaa agtacattta ggtacacggc ctacagaaaa 3301 acagtatgaa actctcgaaa atcaattagc ctttttatgc caacaaggtt tttcactaga 3361 gaatgcatta tatgcactca gcgctgtggg gcattttact ttaggttgcg tattggaaga 3421 tcaagagcat caagtcgcta aagaagaaag ggaaacacct actactgata gtatgccgcc 3481 attattacga caagctatcg aattatttga tcaccaaggt gcagagccag ccttcttatt 3541 cggccttgaa ttgatcatat gcggattaga aaaacaactt aaatgtgaaa gtgggtccgc 3601 tgcaaacgac gaaaactacg ctttagtagc ttaataacac tgatagtgct agtgtagatc 3661 actactagag ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt 3721 tttatctgtt gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt 3781 gggcctttct gcgtttata //