##gff-version 3
##sequence-region BBa_I758009 1 3799
BBa_I758009	.	engineered_region	1	2861	.	+	0	ID=BBa_S03819;Name=BBa_S03819
BBa_I758009	.	engineered_region	1	1992	.	+	0	ID=BBa_S03818;Name=BBa_S03818;Parent=BBa_S03819
BBa_I758009	.	engineered_region	1	1902	.	+	0	ID=BBa_S03817;Name=BBa_S03817;Parent=BBa_S03818
BBa_I758009	.	engineered_region	968	1902	.	+	0	ID=BBa_I6036;Name=BBa_I6036;Parent=BBa_S03817
BBa_I758009	.	terminator	1774	1853	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_I6036
BBa_I758009	.	stem_loop	1785	1828	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I758009	.	engineered_region	1774	1853	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I758009	.	promoter	968	1016	.	+	0	ID=BBa_R0051;Name=BBa_R0051;Parent=BBa_I6036
BBa_I758009	.	promoter	982	987	.	+	0	ID=annotation2023;Name=-35;Parent=BBa_R0051
BBa_I758009	.	promoter	1005	1010	.	+	0	ID=annotation2022;Name=-10;Parent=BBa_R0051
BBa_I758009	.	non_covalent_binding_site	968	984	.	+	0	ID=annotation2025;Name=OR2;Parent=BBa_R0051
BBa_I758009	.	engineered_region	968	1016	.	+	0	ID=annotation7067;Name=BBa_R0051;Parent=BBa_R0051
BBa_I758009	.	non_covalent_binding_site	992	1008	.	+	0	ID=annotation2024;Name=OR1;Parent=BBa_R0051
BBa_I758009	.	ribosome_entry_site	1025	1036	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I6036
BBa_I758009	.	conserved_region	1029	1032	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I758009	.	CDS	1043	1765	.	+	0	ID=BBa_E0030;Name=BBa_E0030;Parent=BBa_I6036
BBa_I758009	.	terminator	1862	1902	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_I6036
BBa_I758009	.	stem_loop	1869	1888	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I758009	.	stop_codon	1895	1895	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I758009	.	polyA_site	1889	1902	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I758009	.	engineered_region	1862	1902	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I758009	.	engineered_region	1	959	.	+	0	ID=BBa_I5010;Name=BBa_I5010;Parent=BBa_S03817
BBa_I758009	.	terminator	914	959	.	+	0	ID=BBa_B0011;Name=BBa_B0011;Parent=BBa_I5010
BBa_I758009	.	engineered_region	914	959	.	+	0	ID=annotation7019;Name=BBa_B0011;Parent=BBa_B0011
BBa_I758009	.	stem_loop	926	948	.	+	0	ID=annotation1683;Name=stem_loop;Parent=BBa_B0011
BBa_I758009	.	promoter	1	54	.	+	0	ID=BBa_R0011;Name=BBa_R0011;Parent=BBa_I5010
BBa_I758009	.	promoter	20	25	.	+	0	ID=annotation2000;Name=-35;Parent=BBa_R0011
BBa_I758009	.	promoter	43	48	.	+	0	ID=annotation2002;Name=-10;Parent=BBa_R0011
BBa_I758009	.	operator	3	19	.	+	0	ID=annotation1999;Name=lac O1;Parent=BBa_R0011
BBa_I758009	.	engineered_region	1	54	.	+	0	ID=annotation7064;Name=BBa_R0011;Parent=BBa_R0011
BBa_I758009	.	operator	26	42	.	+	0	ID=annotation2001;Name=lac O1;Parent=BBa_R0011
BBa_I758009	.	CDS	82	856	.	+	0	ID=BBa_C0051;Name=BBa_C0051;Parent=BBa_I5010
BBa_I758009	.	tag	793	825	.	+	0	ID=annotation23335;Name=LVA;Parent=BBa_C0051
BBa_I758009	.	engineered_tag	832	856	.	+	0	ID=annotation2213991;Name=Help:Barcodes;Parent=BBa_C0051
BBa_I758009	.	CDS	85	792	.	+	0	ID=annotation23334;Name=cI lambda;Parent=BBa_C0051
BBa_I758009	.	ribosome_entry_site	64	75	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I5010
BBa_I758009	.	conserved_region	68	71	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I758009	.	terminator	865	905	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_I5010
BBa_I758009	.	stem_loop	872	891	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I758009	.	stop_codon	898	898	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I758009	.	polyA_site	892	905	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I758009	.	engineered_region	865	905	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I758009	.	promoter	1911	1992	.	+	0	ID=BBa_I12007;Name=BBa_I12007;Parent=BBa_S03818
BBa_I758009	.	sequence_alteration	1969	1975	.	+	0	ID=annotation937704;Name=mutate to obliterate OR3;Parent=BBa_I12007
BBa_I758009	.	non_covalent_binding_site	1943	1959	.	+	0	ID=annotation937702;Name=OR2;Parent=BBa_I12007
BBa_I758009	.	promoter	1958	1963	.	+	0	ID=annotation937703;Name=-35;Parent=BBa_I12007
BBa_I758009	.	promoter	1981	1986	.	+	0	ID=annotation937705;Name=-10;Parent=BBa_I12007
BBa_I758009	.	non_covalent_binding_site	1919	1935	.	+	0	ID=annotation937701;Name=OR1;Parent=BBa_I12007
BBa_I758009	.	engineered_region	2001	2861	.	+	0	ID=BBa_I13507;Name=BBa_I13507;Parent=BBa_S03819
BBa_I758009	.	terminator	2733	2812	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_I13507
BBa_I758009	.	stem_loop	2744	2787	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I758009	.	engineered_region	2733	2812	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I758009	.	CDS	2019	2699	.	+	0	ID=BBa_E1010;Name=BBa_E1010;Parent=BBa_I13507
BBa_I758009	.	CDS	2019	2693	.	+	0	ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010
BBa_I758009	.	engineered_tag	2700	2724	.	+	0	ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010
BBa_I758009	.	ribosome_entry_site	2001	2012	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I13507
BBa_I758009	.	conserved_region	2005	2008	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I758009	.	terminator	2821	2861	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_I13507
BBa_I758009	.	stem_loop	2828	2847	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I758009	.	stop_codon	2854	2854	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I758009	.	polyA_site	2848	2861	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I758009	.	engineered_region	2821	2861	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I758009	.	engineered_region	2870	3799	.	+	0	ID=BBa_I758005;Name=BBa_I758005
BBa_I758009	.	promoter	2870	2951	.	+	0	ID=BBa_I12007;Name=BBa_I12007;Parent=BBa_I758005
BBa_I758009	.	sequence_alteration	2928	2934	.	+	0	ID=annotation937704;Name=mutate to obliterate OR3;Parent=BBa_I12007
BBa_I758009	.	non_covalent_binding_site	2902	2918	.	+	0	ID=annotation937702;Name=OR2;Parent=BBa_I12007
BBa_I758009	.	promoter	2917	2922	.	+	0	ID=annotation937703;Name=-35;Parent=BBa_I12007
BBa_I758009	.	promoter	2940	2945	.	+	0	ID=annotation937705;Name=-10;Parent=BBa_I12007
BBa_I758009	.	non_covalent_binding_site	2878	2894	.	+	0	ID=annotation937701;Name=OR1;Parent=BBa_I12007
BBa_I758009	.	engineered_region	2960	3799	.	+	0	ID=BBa_P0440;Name=BBa_P0440;Parent=BBa_I758005
BBa_I758009	.	terminator	3759	3799	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_P0440
BBa_I758009	.	stem_loop	3766	3785	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I758009	.	stop_codon	3792	3792	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I758009	.	polyA_site	3786	3799	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I758009	.	engineered_region	3759	3799	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I758009	.	terminator	3671	3750	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_P0440
BBa_I758009	.	stem_loop	3682	3725	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I758009	.	engineered_region	3671	3750	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I758009	.	ribosome_entry_site	2960	2971	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_P0440
BBa_I758009	.	conserved_region	2964	2967	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I758009	.	CDS	2978	3637	.	+	0	ID=BBa_C0040;Name=BBa_C0040;Parent=BBa_P0440
BBa_I758009	.	tag	3598	3631	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
BBa_I758009	.	engineered_tag	3638	3662	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
BBa_I758009	.	CDS	2981	3597	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
>BBa_I758009
aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacatactagagaaagaggagaaatact
agatgagcacaaaaaagaaaccattaacacaagagcagcttgaggacgcacgtcgccttaaagcaatttatgaaaaaaa
gaaaaatgaacttggcttatcccaggaatctgtcgcagacaagatggggatggggcagtcaggcgttggtgctttattt
aatggcatcaatgcattaaatgcttataacgccgcattgcttgcaaaaattctcaaagttagcgttgaagaatttagcc
cttcaatcgccagagaaatctacgagatgtatgaagcggttagtatgcagccgtcacttagaagtgagtatgagtaccc
tgttttttctcatgttcaggcagggatgttctcacctgagcttagaacctttaccaaaggtgatgcggagagatgggta
agcacaaccaaaaaagccagtgattctgcattctggcttgaggttgaaggtaattccatgaccgcaccaacaggctcca
agccaagctttcctgacggaatgttaattctcgttgaccctgagcaggctgttgagccaggtgatttctgcatagccag
acttgggggtgatgagtttaccttcaagaaactgatcagggatagcggtcaggtgtttttacaaccactaaacccacag
tacccaatgatcccatgcaatgagagttgttccgttgtggggaaagttatcgctagtcagtggcctgaagagacgtttg
gcgctgcaaacgacgaaaactacgctttagtagcttaataacgctgatagtgctagtgtagatcgctactagagtcaca
ctggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggctt
ttttattattttactagagtaacaccgtgcgtgttgactattttacctctggcggtgataatggttgctactagagaaa
gaggagaaatactagatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcg
acgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcat
ctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccttcggctacggcctgcaatgcttcgcccgc
taccccgaccacatgaagctgcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttct
tcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaa
gggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatc
atggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcg
ccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagctaccagtc
cgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctc
ggcatggacgagctgtacaagtaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcc
tttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcg
tttatatactagaggcaaccattatcaccgccagaggtaaaatagtcaacacgcacggtgttagatatttataaatagt
ggtgatagatttaacgttactagagaaagaggagaaatactagatggcttcctccgaagacgttatcaaagagttcatg
cgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacg
aaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagtt
ccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttc
aaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagt
tcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttggga
agcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggt
ggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgaca
tcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccac
cggtgcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcga
aagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgg
gcctttctgcgtttatatactagaggcaaccattatcaccgccagaggtaaaatagtcaacacgcacggtgttagatat
ttataaatagtggtgatagatttaacgttactagagaaagaggagaaatactagatgtccagattagataaaagtaaag
tgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctagg
tgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagat
aggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagat
gtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcga
aaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcatttt
actttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgc
cgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgat
catatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaa
taacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggc
ctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgc
gtttata