##gff-version 3
##sequence-region BBa_I759004 1 1541
BBa_I759004	.	engineered_region	1	513	.	+	0	ID=BBa_I759001;Name=BBa_I759001
BBa_I759004	.	sequence_feature	55	112	.	+	0	ID=annotation1958469;Name=spacer;Parent=BBa_I759001
BBa_I759004	.	promoter	1	54	.	+	0	ID=annotation1958468;Name=pTet;Parent=BBa_I759001
BBa_I759004	.	sequence_feature	113	513	.	+	0	ID=annotation1958470;Name=N;Parent=BBa_I759001
BBa_I759004	.	engineered_region	522	1541	.	+	0	ID=BBa_S03724;Name=BBa_S03724
BBa_I759004	.	terminator	522	650	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_S03724
BBa_I759004	.	terminator	522	601	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_I759004	.	engineered_region	522	601	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I759004	.	stem_loop	533	576	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I759004	.	terminator	610	650	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_I759004	.	polyA_site	637	650	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I759004	.	engineered_region	610	650	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I759004	.	stop_codon	643	643	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I759004	.	stem_loop	617	636	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I759004	.	engineered_region	659	1541	.	+	0	ID=BBa_S03708;Name=BBa_S03708;Parent=BBa_S03724
BBa_I759004	.	promoter	659	693	.	+	0	ID=BBa_J23116;Name=BBa_J23116;Parent=BBa_S03708
BBa_I759004	.	engineered_region	702	1541	.	+	0	ID=BBa_P0440;Name=BBa_P0440;Parent=BBa_S03708
BBa_I759004	.	terminator	1413	1492	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_P0440
BBa_I759004	.	engineered_region	1413	1492	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I759004	.	stem_loop	1424	1467	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I759004	.	ribosome_entry_site	702	713	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_P0440
BBa_I759004	.	conserved_region	706	709	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I759004	.	terminator	1501	1541	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_P0440
BBa_I759004	.	polyA_site	1528	1541	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I759004	.	engineered_region	1501	1541	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I759004	.	stop_codon	1534	1534	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I759004	.	stem_loop	1508	1527	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I759004	.	CDS	720	1379	.	+	0	ID=BBa_C0040;Name=BBa_C0040;Parent=BBa_P0440
BBa_I759004	.	tag	1340	1373	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
BBa_I759004	.	CDS	723	1339	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
BBa_I759004	.	engineered_tag	1380	1404	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
>BBa_I759004
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcacgtgcacaattcactgagtcatatga
tcgatttgggtattaaagaggagaaacaattgatgtgccaatcgcggggggttttcgttcaggactacaactgccacac
accaccaaagctaactgacaggagaatccagatggatgcacaaacacgccgccgcgaacgtcgcgcagagaaacaggct
caatggaaagcagcaaatcccctgttggttggggtaagcgcaaaaccagttaaccgccctattctctcgctgaatcgca
aaccgaaatcacgagtagaaagcgcactaaatccgatagaccttacagtgctggctgaataccacaaacagattgaaag
caacctgcaacgtattgagcgcaagaatcagcgcacatggtacagcaagcctggcgaacgcggcataacatgcagtgga
cgccagaaaattaagggaaaatcgattcctcttatctagtactagagccaggcatcaaataaaacgaaaggctcagtcg
aaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtg
ggcctttctgcgtttatatactagagttgacagctagctcagtcctagggactatgctagctactagagaaagaggaga
aatactagatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaagg
tttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggct
ttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaag
attttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtac
acggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaat
gcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaag
aagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgc
agagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgct
gcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagccaggcatca
aataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagt
cacactggctcaccttcgggtgggcctttctgcgtttata