##gff-version 3
##sequence-region BBa_I759005 1 1340
BBa_I759005	.	engineered_region	321	1340	.	+	0	ID=BBa_S03724;Name=BBa_S03724
BBa_I759005	.	terminator	321	449	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_S03724
BBa_I759005	.	terminator	409	449	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_I759005	.	polyA_site	436	449	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I759005	.	stem_loop	416	435	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I759005	.	stop_codon	442	442	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I759005	.	engineered_region	409	449	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I759005	.	terminator	321	400	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_I759005	.	stem_loop	332	375	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I759005	.	engineered_region	321	400	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I759005	.	engineered_region	458	1340	.	+	0	ID=BBa_S03708;Name=BBa_S03708;Parent=BBa_S03724
BBa_I759005	.	promoter	458	492	.	+	0	ID=BBa_J23116;Name=BBa_J23116;Parent=BBa_S03708
BBa_I759005	.	engineered_region	501	1340	.	+	0	ID=BBa_P0440;Name=BBa_P0440;Parent=BBa_S03708
BBa_I759005	.	terminator	1212	1291	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_P0440
BBa_I759005	.	stem_loop	1223	1266	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I759005	.	engineered_region	1212	1291	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I759005	.	ribosome_entry_site	501	512	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_P0440
BBa_I759005	.	conserved_region	505	508	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I759005	.	terminator	1300	1340	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_P0440
BBa_I759005	.	polyA_site	1327	1340	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I759005	.	stem_loop	1307	1326	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I759005	.	stop_codon	1333	1333	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I759005	.	engineered_region	1300	1340	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I759005	.	CDS	519	1178	.	+	0	ID=BBa_C0040;Name=BBa_C0040;Parent=BBa_P0440
BBa_I759005	.	engineered_tag	1179	1203	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
BBa_I759005	.	tag	1139	1172	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
BBa_I759005	.	CDS	522	1138	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
BBa_I759005	.	engineered_region	1	312	.	+	0	ID=BBa_I759002;Name=BBa_I759002
BBa_I759005	.	promoter	1	54	.	+	0	ID=annotation1957527;Name=pTet;Parent=BBa_I759002
BBa_I759005	.	sequence_feature	112	312	.	+	0	ID=annotation1957528;Name=cro gene;Parent=BBa_I759002
BBa_I759005	.	sequence_feature	55	111	.	+	0	ID=annotation1957529;Name=spacer;Parent=BBa_I759002
>BBa_I759005
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcacgtgcacaattcactgagtcatatga
tcgatttgggtattaaagaggagaaacaattgatggaacaacgcataaccctgaaagattatgcaatgcgctttgggca
aaccaagacagctaaagatctcggcgtatatcaaagcgcgatcaacaaggccattcatgcaggccgaaagattttttta
actataaacgctgatggaagcgtttatgcggaagaggtaaagcccttcccgagtaacaaaaaaacaacagcataatact
agagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacg
ctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagttgacagctagctcagt
cctagggactatgctagctactagagaaagaggagaaatactagatgtccagattagataaaagtaaagtgattaacag
cgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcag
cctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccata
ctcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttact
aagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaatta
gcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggtt
gcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattatt
acgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcgga
ttagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgat
agtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgtttt
atctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata