LOCUS       BBa_I759007             1859 bp    DNA     linear       22-Dec-2024
DEFINITION  tet-regulated GFP (Weak tetR promoter)
ACCESSION   BBa_I759007
VERSION     BBa_I759007.1  
FEATURES             Location/Qualifiers
     misc_feature    1..831
                     /label=BBa_I759006
     stem_loop       851..894
                     /label=stem_loop
     misc_feature    840..919
                     /label=BBa_B0010
     terminator      840..919
                     /label=BBa_B0010
     stem_loop       935..954
                     /label=T7 TE
     misc_feature    961..961
                     /label=stop
     polyA_site      955..968
                     /label=polya
     misc_feature    928..968
                     /label=BBa_B0012
     terminator      928..968
                     /label=BBa_B0012
     terminator      840..968
                     /label=BBa_B0015
     promoter        977..1011
                     /label=BBa_J23116
     misc_feature    1024..1027
                     /label=conserved
     RBS             1020..1031
                     /label=BBa_B0034
     CDS             1041..1657
                     /label=tetR
     misc_feature    1658..1691
                     /label=SsrA
     misc_feature    1698..1722
                     /label=Help:Barcodes
     CDS             1038..1697
                     /label=BBa_C0040
     stem_loop       1742..1785
                     /label=stem_loop
     misc_feature    1731..1810
                     /label=BBa_B0010
     terminator      1731..1810
                     /label=BBa_B0010
     stem_loop       1826..1845
                     /label=T7 TE
     misc_feature    1852..1852
                     /label=stop
     polyA_site      1846..1859
                     /label=polya
     misc_feature    1819..1859
                     /label=BBa_B0012
     terminator      1819..1859
                     /label=BBa_B0012
     misc_feature    1020..1859
                     /label=BBa_P0440
     misc_feature    977..1859
                     /label=BBa_S03708
     misc_feature    840..1859
                     /label=BBa_S03724
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcacgtgcac
       61 aattcactga gtcatatgat cgatttgggt attaaagagg agaaacaatt gatgcgtaaa
      121 ggagaagaac ttttcactgg agttgtccca attcttgttg aattagatgg tgatgttaat
      181 gggcacaaat tttctgtcag tggagagggt gaaggtgatg caacatacgg aaaacttacc
      241 cttaaattta tttgcactac tggaaaacta cctgttccat ggccaacact tgtcactact
      301 ttcggttatg gtgttcaatg ctttgcgaga tacccagatc atatgaaaca gcatgacttt
      361 ttcaagagtg ccatgcccga aggttatgta caggaaagaa ctatattttt caaagatgac
      421 gggaactaca agacacgtgc tgaagtcaag tttgaaggtg atacccttgt taatagaatc
      481 gagttaaaag gtattgattt taaagaagat ggaaacattc ttggacacaa attggaatac
      541 aactataact cacacaatgt atacatcatg gcagacaaac aaaagaatgg aatcaaagtt
      601 aacttcaaaa ttagacacaa cattgaagat ggaagcgttc aactagcaga ccattatcaa
      661 caaaatactc caattggcga tggccctgtc cttttaccag acaaccatta cctgtccaca
      721 caatctgccc tttcgaaaga tcccaacgaa aagagagacc acatggtcct tcttgagttt
      781 gtaacagctg ctgggattac acatggcatg gatgaactat acaaataata atactagagc
      841 caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg
      901 tttgtcggtg aacgctctct actagagtca cactggctca ccttcgggtg ggcctttctg
      961 cgtttatata ctagagttga cagctagctc agtcctaggg actatgctag ctactagaga
     1021 aagaggagaa atactagatg tccagattag ataaaagtaa agtgattaac agcgcattag
     1081 agctgcttaa tgaggtcgga atcgaaggtt taacaacccg taaactcgcc cagaagctag
     1141 gtgtagagca gcctacattg tattggcatg taaaaaataa gcgggctttg ctcgacgcct
     1201 tagccattga gatgttagat aggcaccata ctcacttttg ccctttagaa ggggaaagct
     1261 ggcaagattt tttacgtaat aacgctaaaa gttttagatg tgctttacta agtcatcgcg
     1321 atggagcaaa agtacattta ggtacacggc ctacagaaaa acagtatgaa actctcgaaa
     1381 atcaattagc ctttttatgc caacaaggtt tttcactaga gaatgcatta tatgcactca
     1441 gcgctgtggg gcattttact ttaggttgcg tattggaaga tcaagagcat caagtcgcta
     1501 aagaagaaag ggaaacacct actactgata gtatgccgcc attattacga caagctatcg
     1561 aattatttga tcaccaaggt gcagagccag ccttcttatt cggccttgaa ttgatcatat
     1621 gcggattaga aaaacaactt aaatgtgaaa gtgggtccgc tgcaaacgac gaaaactacg
     1681 ctttagtagc ttaataacac tgatagtgct agtgtagatc actactagag ccaggcatca
     1741 aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt
     1801 gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct gcgtttata
//