##gff-version 3
##sequence-region BBa_I759028 1 1766
BBa_I759028	.	engineered_region	747	1766	.	+	0	ID=BBa_S03724;Name=BBa_S03724
BBa_I759028	.	terminator	747	875	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_S03724
BBa_I759028	.	terminator	835	875	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_I759028	.	polyA_site	862	875	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I759028	.	stem_loop	842	861	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I759028	.	stop_codon	868	868	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I759028	.	engineered_region	835	875	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I759028	.	terminator	747	826	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_I759028	.	stem_loop	758	801	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I759028	.	engineered_region	747	826	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I759028	.	engineered_region	884	1766	.	+	0	ID=BBa_S03708;Name=BBa_S03708;Parent=BBa_S03724
BBa_I759028	.	promoter	884	918	.	+	0	ID=BBa_J23116;Name=BBa_J23116;Parent=BBa_S03708
BBa_I759028	.	engineered_region	927	1766	.	+	0	ID=BBa_P0440;Name=BBa_P0440;Parent=BBa_S03708
BBa_I759028	.	CDS	945	1604	.	+	0	ID=BBa_C0040;Name=BBa_C0040;Parent=BBa_P0440
BBa_I759028	.	CDS	948	1564	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
BBa_I759028	.	tag	1565	1598	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
BBa_I759028	.	engineered_tag	1605	1629	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
BBa_I759028	.	terminator	1726	1766	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_P0440
BBa_I759028	.	polyA_site	1753	1766	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I759028	.	stem_loop	1733	1752	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I759028	.	stop_codon	1759	1759	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I759028	.	engineered_region	1726	1766	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I759028	.	terminator	1638	1717	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_P0440
BBa_I759028	.	stem_loop	1649	1692	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I759028	.	engineered_region	1638	1717	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I759028	.	ribosome_entry_site	927	938	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_P0440
BBa_I759028	.	conserved_region	931	934	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I759028	.	engineered_region	1	738	.	+	0	ID=BBa_I759012;Name=BBa_I759012
BBa_I759028	.	sequence_feature	107	738	.	+	0	ID=annotation1958475;Name=Q;Parent=BBa_I759012
BBa_I759028	.	promoter	1	54	.	+	0	ID=annotation1958473;Name=pTet;Parent=BBa_I759012
BBa_I759028	.	sequence_feature	55	106	.	+	0	ID=annotation1958474;Name=cis4 + RBS;Parent=BBa_I759012
>BBa_I759028
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcacgtgcacattgattcacctctttgtg
ttgggtattaaagaggagaaacaattgatgagactcgaaagcgtagctaaatttcattcgccaaaaagcccgatgatga
gcgactcaccacgggccacggcttctgactctctttccggtactgatgtgatggctgctatggggatggcgcaatcaca
agccggattcggtatggctgcattctgcggtaagcacgaactcagccagaacgacaaacaaaaggctatcaactatctg
atgcaatttgcacacaaggtatcggggaaataccgtggtgtggcaaagcttgaaggaaatactaaggcaaaggtactgc
aagtgctcgcaacattcgcttatgcggattattgccgtagtgccgcgacgccgggggcaagatgcagagattgccatgg
tacaggccgtgcggttgatattgccaaaacagagctgtgggggagagttgtcgagaaagagtgcggaagatgcaaaggc
gtcggctattcaaggatgccagcaagcgcagcatatcgcgctgtgacgatgctaatcccaaaccttacccaacccacct
ggtcacgcactgttaagccgctgtatgacgctctggtggtgcaatgccacaaagaagagtcaatcgcagacaacatttt
gaatgcggtcacacgttagtactagagtactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcc
tttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcg
tttatatactagagttgacagctagctcagtcctagggactatgctagctactagagaaagaggagaaatactagatgt
ccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccg
taaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgcc
ttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgta
ataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacaga
aaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgca
ctcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaa
cacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagcctt
cttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaa
aactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaa
ggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctca
ccttcgggtgggcctttctgcgtttata