LOCUS BBa_I759035 1857 bp DNA linear 22-Nov-2024 DEFINITION cis2-repressed, tet-regulated YFP ACCESSION BBa_I759035 VERSION BBa_I759035.1 FEATURES Location/Qualifiers promoter 1..54 /label=pTet misc_feature 55..106 /label=cis2 + RBS misc_feature 107..829 /label=YFP misc_feature 1..829 /label=BBa_I759034 stem_loop 849..892 /label=stem_loop misc_feature 838..917 /label=BBa_B0010 terminator 838..917 /label=BBa_B0010 stem_loop 933..952 /label=T7 TE misc_feature 959..959 /label=stop polyA_site 953..966 /label=polya misc_feature 926..966 /label=BBa_B0012 terminator 926..966 /label=BBa_B0012 promoter 975..1009 /label=BBa_J23116 misc_feature 1022..1025 /label=conserved RBS 1018..1029 /label=BBa_B0034 CDS 1039..1655 /label=tetR misc_feature 1656..1689 /label=SsrA misc_feature 1696..1720 /label=Help:Barcodes CDS 1036..1695 /label=BBa_C0040 stem_loop 1740..1783 /label=stem_loop misc_feature 1729..1808 /label=BBa_B0010 terminator 1729..1808 /label=BBa_B0010 stem_loop 1824..1843 /label=T7 TE misc_feature 1850..1850 /label=stop polyA_site 1844..1857 /label=polya misc_feature 1817..1857 /label=BBa_B0012 terminator 1817..1857 /label=BBa_B0012 ORIGIN 1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcacgtgcac 61 attgtttcac ctcttggatt tgggtattaa agaggagaaa caattgatgg tgagcaaggg 121 cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg 181 ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct 241 gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccacctt 301 cggctacggc ctgcaatgct tcgcccgcta ccccgaccac atgaagctgc acgacttctt 361 caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg 421 caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga 481 gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa 541 ctacaacagc cacaacgtct atatcatggc cgacaagcag aagaacggca tcaaggtgaa 601 cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc actaccagca 661 gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagctacca 721 gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt 781 gaccgccgcc gggatcactc tcggcatgga cgagctgtac aagtaataat actagagcca 841 ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt 901 tgtcggtgaa cgctctctac tagagtcaca ctggctcacc ttcgggtggg cctttctgcg 961 tttatatact agagttgaca gctagctcag tcctagggac tatgctagct actagagaaa 1021 gaggagaaat actagatgtc cagattagat aaaagtaaag tgattaacag cgcattagag 1081 ctgcttaatg aggtcggaat cgaaggttta acaacccgta aactcgccca gaagctaggt 1141 gtagagcagc ctacattgta ttggcatgta aaaaataagc gggctttgct cgacgcctta 1201 gccattgaga tgttagatag gcaccatact cacttttgcc ctttagaagg ggaaagctgg 1261 caagattttt tacgtaataa cgctaaaagt tttagatgtg ctttactaag tcatcgcgat 1321 ggagcaaaag tacatttagg tacacggcct acagaaaaac agtatgaaac tctcgaaaat 1381 caattagcct ttttatgcca acaaggtttt tcactagaga atgcattata tgcactcagc 1441 gctgtggggc attttacttt aggttgcgta ttggaagatc aagagcatca agtcgctaaa 1501 gaagaaaggg aaacacctac tactgatagt atgccgccat tattacgaca agctatcgaa 1561 ttatttgatc accaaggtgc agagccagcc ttcttattcg gccttgaatt gatcatatgc 1621 ggattagaaa aacaacttaa atgtgaaagt gggtccgctg caaacgacga aaactacgct 1681 ttagtagctt aataacactg atagtgctag tgtagatcac tactagagcc aggcatcaaa 1741 taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga 1801 acgctctcta ctagagtcac actggctcac cttcgggtgg gcctttctgc gtttata // LOCUS BBa_I759014 1857 bp DNA linear 22-Nov-2024 DEFINITION cis2-repressed, tet-regulated YFP ACCESSION BBa_I759014 VERSION BBa_I759014.1 FEATURES Location/Qualifiers promoter 1..54 /label=pTet misc_feature 55..106 /label=cis2 + RBS misc_feature 107..829 /label=YFP misc_feature 1..829 /label=BBa_I759034 stem_loop 849..892 /label=stem_loop misc_feature 838..917 /label=BBa_B0010 terminator 838..917 /label=BBa_B0010 stem_loop 933..952 /label=T7 TE misc_feature 959..959 /label=stop polyA_site 953..966 /label=polya misc_feature 926..966 /label=BBa_B0012 terminator 926..966 /label=BBa_B0012 terminator 838..966 /label=BBa_B0015 promoter 975..1009 /label=BBa_J23116 misc_feature 1022..1025 /label=conserved RBS 1018..1029 /label=BBa_B0034 CDS 1039..1655 /label=tetR misc_feature 1656..1689 /label=SsrA misc_feature 1696..1720 /label=Help:Barcodes CDS 1036..1695 /label=BBa_C0040 stem_loop 1740..1783 /label=stem_loop misc_feature 1729..1808 /label=BBa_B0010 terminator 1729..1808 /label=BBa_B0010 stem_loop 1824..1843 /label=T7 TE misc_feature 1850..1850 /label=stop polyA_site 1844..1857 /label=polya misc_feature 1817..1857 /label=BBa_B0012 terminator 1817..1857 /label=BBa_B0012 misc_feature 1018..1857 /label=BBa_P0440 misc_feature 975..1857 /label=BBa_S03708 misc_feature 838..1857 /label=BBa_S03724 ORIGIN 1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcacgtgcac 61 attgtttcac ctcttggatt tgggtattaa agaggagaaa caattgatgg tgagcaaggg 121 cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg 181 ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct 241 gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccacctt 301 cggctacggc ctgcaatgct tcgcccgcta ccccgaccac atgaagctgc acgacttctt 361 caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg 421 caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga 481 gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa 541 ctacaacagc cacaacgtct atatcatggc cgacaagcag aagaacggca tcaaggtgaa 601 cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc actaccagca 661 gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagctacca 721 gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt 781 gaccgccgcc gggatcactc tcggcatgga cgagctgtac aagtaataat actagagcca 841 ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt 901 tgtcggtgaa cgctctctac tagagtcaca ctggctcacc ttcgggtggg cctttctgcg 961 tttatatact agagttgaca gctagctcag tcctagggac tatgctagct actagagaaa 1021 gaggagaaat actagatgtc cagattagat aaaagtaaag tgattaacag cgcattagag 1081 ctgcttaatg aggtcggaat cgaaggttta acaacccgta aactcgccca gaagctaggt 1141 gtagagcagc ctacattgta ttggcatgta aaaaataagc gggctttgct cgacgcctta 1201 gccattgaga tgttagatag gcaccatact cacttttgcc ctttagaagg ggaaagctgg 1261 caagattttt tacgtaataa cgctaaaagt tttagatgtg ctttactaag tcatcgcgat 1321 ggagcaaaag tacatttagg tacacggcct acagaaaaac agtatgaaac tctcgaaaat 1381 caattagcct ttttatgcca acaaggtttt tcactagaga atgcattata tgcactcagc 1441 gctgtggggc attttacttt aggttgcgta ttggaagatc aagagcatca agtcgctaaa 1501 gaagaaaggg aaacacctac tactgatagt atgccgccat tattacgaca agctatcgaa 1561 ttatttgatc accaaggtgc agagccagcc ttcttattcg gccttgaatt gatcatatgc 1621 ggattagaaa aacaacttaa atgtgaaagt gggtccgctg caaacgacga aaactacgct 1681 ttagtagctt aataacactg atagtgctag tgtagatcac tactagagcc aggcatcaaa 1741 taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga 1801 acgctctcta ctagagtcac actggctcac cttcgggtgg gcctttctgc gtttata //