LOCUS BBa_I759041 1861 bp DNA linear 26-Dec-2024 DEFINITION cis5-repressed, tet-regulated YFP ACCESSION BBa_I759041 VERSION BBa_I759041.1 FEATURES Location/Qualifiers promoter 1..54 /label=pTet misc_feature 55..110 /label=cis5 + RBS misc_feature 111..833 /label=YFP misc_feature 1..833 /label=BBa_I759040 stem_loop 853..896 /label=stem_loop misc_feature 842..921 /label=BBa_B0010 terminator 842..921 /label=BBa_B0010 stem_loop 937..956 /label=T7 TE misc_feature 963..963 /label=stop polyA_site 957..970 /label=polya misc_feature 930..970 /label=BBa_B0012 terminator 930..970 /label=BBa_B0012 promoter 979..1013 /label=BBa_J23116 misc_feature 1026..1029 /label=conserved RBS 1022..1033 /label=BBa_B0034 CDS 1043..1659 /label=tetR misc_feature 1660..1693 /label=SsrA misc_feature 1700..1724 /label=Help:Barcodes CDS 1040..1699 /label=BBa_C0040 stem_loop 1744..1787 /label=stem_loop misc_feature 1733..1812 /label=BBa_B0010 terminator 1733..1812 /label=BBa_B0010 stem_loop 1828..1847 /label=T7 TE misc_feature 1854..1854 /label=stop polyA_site 1848..1861 /label=polya misc_feature 1821..1861 /label=BBa_B0012 terminator 1821..1861 /label=BBa_B0012 ORIGIN 1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcacgtgcac 61 aactgtttca cctcttgctt ttgggtaaga cagaagagga gaaacaattg atggtgagca 121 agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa 181 acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga 241 ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca 301 ccttcggcta cggcctgcaa tgcttcgccc gctaccccga ccacatgaag ctgcacgact 361 tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg 421 acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca 481 tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt 541 acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac ggcatcaagg 601 tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc 661 agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagct 721 accagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt 781 tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagtaa taatactaga 841 gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt 901 tgtttgtcgg tgaacgctct ctactagagt cacactggct caccttcggg tgggcctttc 961 tgcgtttata tactagagtt gacagctagc tcagtcctag ggactatgct agctactaga 1021 gaaagaggag aaatactaga tgtccagatt agataaaagt aaagtgatta acagcgcatt 1081 agagctgctt aatgaggtcg gaatcgaagg tttaacaacc cgtaaactcg cccagaagct 1141 aggtgtagag cagcctacat tgtattggca tgtaaaaaat aagcgggctt tgctcgacgc 1201 cttagccatt gagatgttag ataggcacca tactcacttt tgccctttag aaggggaaag 1261 ctggcaagat tttttacgta ataacgctaa aagttttaga tgtgctttac taagtcatcg 1321 cgatggagca aaagtacatt taggtacacg gcctacagaa aaacagtatg aaactctcga 1381 aaatcaatta gcctttttat gccaacaagg tttttcacta gagaatgcat tatatgcact 1441 cagcgctgtg gggcatttta ctttaggttg cgtattggaa gatcaagagc atcaagtcgc 1501 taaagaagaa agggaaacac ctactactga tagtatgccg ccattattac gacaagctat 1561 cgaattattt gatcaccaag gtgcagagcc agccttctta ttcggccttg aattgatcat 1621 atgcggatta gaaaaacaac ttaaatgtga aagtgggtcc gctgcaaacg acgaaaacta 1681 cgctttagta gcttaataac actgatagtg ctagtgtaga tcactactag agccaggcat 1741 caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg 1801 gtgaacgctc tctactagag tcacactggc tcaccttcgg gtgggccttt ctgcgtttat 1861 a // LOCUS BBa_I759017 1861 bp DNA linear 26-Dec-2024 DEFINITION cis5-repressed, tet-regulated YFP ACCESSION BBa_I759017 VERSION BBa_I759017.1 FEATURES Location/Qualifiers promoter 1..54 /label=pTet misc_feature 55..110 /label=cis5 + RBS misc_feature 111..833 /label=YFP misc_feature 1..833 /label=BBa_I759040 stem_loop 853..896 /label=stem_loop misc_feature 842..921 /label=BBa_B0010 terminator 842..921 /label=BBa_B0010 stem_loop 937..956 /label=T7 TE misc_feature 963..963 /label=stop polyA_site 957..970 /label=polya misc_feature 930..970 /label=BBa_B0012 terminator 930..970 /label=BBa_B0012 terminator 842..970 /label=BBa_B0015 promoter 979..1013 /label=BBa_J23116 misc_feature 1026..1029 /label=conserved RBS 1022..1033 /label=BBa_B0034 CDS 1043..1659 /label=tetR misc_feature 1660..1693 /label=SsrA misc_feature 1700..1724 /label=Help:Barcodes CDS 1040..1699 /label=BBa_C0040 stem_loop 1744..1787 /label=stem_loop misc_feature 1733..1812 /label=BBa_B0010 terminator 1733..1812 /label=BBa_B0010 stem_loop 1828..1847 /label=T7 TE misc_feature 1854..1854 /label=stop polyA_site 1848..1861 /label=polya misc_feature 1821..1861 /label=BBa_B0012 terminator 1821..1861 /label=BBa_B0012 misc_feature 1022..1861 /label=BBa_P0440 misc_feature 979..1861 /label=BBa_S03708 misc_feature 842..1861 /label=BBa_S03724 ORIGIN 1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcacgtgcac 61 aactgtttca cctcttgctt ttgggtaaga cagaagagga gaaacaattg atggtgagca 121 agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa 181 acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga 241 ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca 301 ccttcggcta cggcctgcaa tgcttcgccc gctaccccga ccacatgaag ctgcacgact 361 tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg 421 acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca 481 tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt 541 acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac ggcatcaagg 601 tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc 661 agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagct 721 accagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt 781 tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagtaa taatactaga 841 gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt 901 tgtttgtcgg tgaacgctct ctactagagt cacactggct caccttcggg tgggcctttc 961 tgcgtttata tactagagtt gacagctagc tcagtcctag ggactatgct agctactaga 1021 gaaagaggag aaatactaga tgtccagatt agataaaagt aaagtgatta acagcgcatt 1081 agagctgctt aatgaggtcg gaatcgaagg tttaacaacc cgtaaactcg cccagaagct 1141 aggtgtagag cagcctacat tgtattggca tgtaaaaaat aagcgggctt tgctcgacgc 1201 cttagccatt gagatgttag ataggcacca tactcacttt tgccctttag aaggggaaag 1261 ctggcaagat tttttacgta ataacgctaa aagttttaga tgtgctttac taagtcatcg 1321 cgatggagca aaagtacatt taggtacacg gcctacagaa aaacagtatg aaactctcga 1381 aaatcaatta gcctttttat gccaacaagg tttttcacta gagaatgcat tatatgcact 1441 cagcgctgtg gggcatttta ctttaggttg cgtattggaa gatcaagagc atcaagtcgc 1501 taaagaagaa agggaaacac ctactactga tagtatgccg ccattattac gacaagctat 1561 cgaattattt gatcaccaag gtgcagagcc agccttctta ttcggccttg aattgatcat 1621 atgcggatta gaaaaacaac ttaaatgtga aagtgggtcc gctgcaaacg acgaaaacta 1681 cgctttagta gcttaataac actgatagtg ctagtgtaga tcactactag agccaggcat 1741 caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg 1801 gtgaacgctc tctactagag tcacactggc tcaccttcgg gtgggccttt ctgcgtttat 1861 a //