LOCUS BBa_I760010 1737 bp DNA linear 25-Nov-2024 DEFINITION Trigger-1 ACCESSION BBa_I760010 VERSION BBa_I760010.1 FEATURES Location/Qualifiers promoter 1..16 /label=BBa_I760005 misc_feature 25..67 /label=OR2 misc_feature 32..45 /label=OR2 misc_feature 54..67 /label=OR1 promoter 25..30 /label=-35 promoter 48..53 /label=-10 promoter 26..31 /label=-35 misc_feature 59..61 /label=start misc_feature 25..70 /label=BBa_R0052 promoter 25..70 /label=BBa_R0052 misc_feature 83..86 /label=conserved RBS 79..90 /label=BBa_B0034 CDS 100..807 /label=cI lambda misc_feature 808..840 /label=LVA misc_feature 847..871 /label=Help:Barcodes CDS 97..846 /label=BBa_C0051 misc_feature 79..871 /label=BBa_J06550 CDS 878..1585 /label=mCherry misc_feature 1229..1229 /label=C->T (removing PstI site) misc_feature 1586..1594 /label=LVA misc_feature 878..1600 /label=BBa_J06505 misc_feature 1609..1649 /label=B0012 misc_feature 1658..1737 /label=B0010 terminator 1609..1737 /label=BBa_B0025 ORIGIN 1 atgacaaaat tgtcattact agagttgaca aacaagatac attgtatgaa aatacaagaa 61 agtttgttga tactagagaa agaggagaaa tactagatga gcacaaaaaa gaaaccatta 121 acacaagagc agcttgagga cgcacgtcgc cttaaagcaa tttatgaaaa aaagaaaaat 181 gaacttggct tatcccagga atctgtcgca gacaagatgg ggatggggca gtcaggcgtt 241 ggtgctttat ttaatggcat caatgcatta aatgcttata acgccgcatt gcttgcaaaa 301 attctcaaag ttagcgttga agaatttagc ccttcaatcg ccagagaaat ctacgagatg 361 tatgaagcgg ttagtatgca gccgtcactt agaagtgagt atgagtaccc tgttttttct 421 catgttcagg cagggatgtt ctcacctgag cttagaacct ttaccaaagg tgatgcggag 481 agatgggtaa gcacaaccaa aaaagccagt gattctgcat tctggcttga ggttgaaggt 541 aattccatga ccgcaccaac aggctccaag ccaagctttc ctgacggaat gttaattctc 601 gttgaccctg agcaggctgt tgagccaggt gatttctgca tagccagact tgggggtgat 661 gagtttacct tcaagaaact gatcagggat agcggtcagg tgtttttaca accactaaac 721 ccacagtacc caatgatccc atgcaatgag agttgttccg ttgtggggaa agttatcgct 781 agtcagtggc ctgaagagac gtttggcgct gcaaacgacg aaaactacgc tttagtagct 841 taataacgct gatagtgcta gtgtagatcg ctactagatg gtgagcaagg gcgaggagga 901 taacatggcc atcatcaagg agttcatgcg cttcaaggtg cacatggagg gctccgtgaa 961 cggccacgag ttcgagatcg agggcgaggg cgagggccgc ccctacgagg gcacccagac 1021 cgccaagctg aaggtgacca agggtggccc cctgcccttc gcctgggaca tcctgtcccc 1081 tcagttcatg tacggctcca aggcctacgt gaagcacccc gccgacatcc ccgactactt 1141 gaagctgtcc ttccccgagg gcttcaagtg ggagcgcgtg atgaacttcg aggacggcgg 1201 cgtggtgacc gtgacccagg actcctcctt gcaggacggc gagttcatct acaaggtgaa 1261 gctgcgcggc accaacttcc cctccgacgg ccccgtaatg cagaagaaga ccatgggctg 1321 ggaggcctcc tccgagcgga tgtaccccga ggacggcgcc ctgaagggcg agatcaagca 1381 gaggctgaag ctgaaggacg gcggccacta cgacgctgag gtcaagacca cctacaaggc 1441 caagaagccc gtgcagctgc ccggcgccta caacgtcaac atcaagttgg acatcacctc 1501 ccacaacgag gactacacca tcgtggaaca gtacgaacgc gccgagggcc gccactccac 1561 cggcggcatg gacgagctgt acaagttagt agcttaataa tactagagta taaacgcaga 1621 aaggcccacc cgaaggtgag ccagtgtgac tctagtagag agcgttcacc gacaaacaac 1681 agataaaacg aaaggcccag tctttcgact gagcctttcg ttttatttga tgcctgg //