BBa_I761004 1 BBa_I761004 TAT signal+IDE 2007-10-18T11:00:00Z 2015-08-31T04:08:08Z NCBI accession number: BC041675 K12 E. coli genomic DNA Twin arginin signal peptide is an efficient protein export signal sequence of E. coli. The purpose of this construct is to allow E. coli export mouse insulin degradation enzyme(IDE) automatically, allowing controlled insulin degradation of extracellular insulin. false false _140_ 0 2031 9 Not in stock false To combine TAT signal and mouse IDE, we used two separate PCR reaction to amplify each DNA fragment. We design the reverse primer of TAT signal and the forward primer of mouse IDE to be complement to each other, so when we mixed the product of each PCR reaction together, than used the forward primer of TAT signal and the reverse primer of mouse IDE to conduct PCR reaction, we can isolate the combine DNA product of TAT signal and mouse IDE. (Method devised by Dr. Chang) false 2007 NYMU_Taiwan iGEM Team annotation1949763 1 Mouse insulin degradation enzyme range1949763 1 89 3150 annotation1949762 1 TAT peptide export signal range1949762 1 4 88 BBa_I761001 1 OmpR_BS OmpR binding site 2007-09-15T11:00:00Z 2015-08-31T04:08:08Z BBa_R0082 OmpR binding site. When position behind a promotor, it will turns that promotor into a OmpR controlled promotor. OmpR will function as an inhibitor of that promotor. false false _140_ 0 2031 9 Not in stock false This part was derived from BBa_R0082 by PCR. false 2007 NYMU_Taiwan iGEM Team annotation1944248 1 C1 OmpR range1944248 1 2 19 annotation1944250 1 C3 OmpR range1944250 1 43 60 annotation1944249 1 C2 OmpR range1944249 1 22 40 BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_I761006 1 BBa_I761006 Glucose and cinr+cinl controlled mouse insulin degradation enzyme generator 2007-10-18T11:00:00Z 2015-08-31T04:08:08Z *BBa_R0078 *BBa_I761001 *BBa_B0034 *BBa_I761004 This generator is under control of extracellular glucose concentration and insulin activation state. High extracellular glucose concentration will turn off the generator by the binding of phosphorylated OmpR. Unless BBa_I761007 has respond to prior high extracellular glucose concentration, this generator will not turn on. This is achieved by the control of cinr and cinl. After mouse insulin degradation enzyme is generated, it will be exported and can degrade extracellular insulin. false false _140_ 0 2031 9 Not in stock false This generator will turn on if and only if the extracellular glucose concentration is low and BBa_I761007 has been turned on at least once. The basic concept is that when extracellular glucose concentration is low, OmpR dephosphrylated and dissociate from its binding site. When this occurs, BBa_I761007 turns off, but the inhibitory effect of phosphorylated OmpR on this generator is relieved. While the mRNA synthesis of cinr and cinl has turned off, there are still some active CinR and CinL presented in E. coli as protein. Therefore, the generator will turn on due to the activation of the promotor BBa_R0078. Hence our goal is achieved. false 2007 NYMU_Taiwan iGEM Team component1957809 1 BBa_I761001 component1957814 1 BBa_I761004 component1957811 1 BBa_B0034 component1957805 1 BBa_R0078 annotation1957814 1 BBa_I761004 range1957814 1 322 3477 annotation1957811 1 BBa_B0034 range1957811 1 304 315 annotation1957809 1 BBa_I761001 range1957809 1 234 295 annotation1957805 1 BBa_R0078 range1957805 1 1 225 BBa_R0078 1 cinR Promoter (cinR and HSL regulated) 2004-01-29T12:00:00Z 2015-05-08T01:14:15Z Rhizobium leguminosarum Released HQ 2013 false false _1_ 0 24 7 In stock false true Drew Endy annotation318234 1 unidentified/uncharacterized CinR dependent range318234 1 1 220 annotation318244 1 stem_loop range318244 1 35 74 annotation318250 1 stem_loop range318250 1 91 136 BBa_R0078_sequence 1 ccctttgtgcgtccaaacggacgcacggcgctctaaagcgggtcgcgatctttcagattcgctcctcgcgctttcagtctttgttttggcgcatgtcgttatcgcaaaaccgctgcacacttttgcgcgacatgctctgatccccctcatctgggggggcctatctgagggaatttccgatccggctcgcctgaaccattctgctttccacgaacttgaaaacgc BBa_I761001_sequence 1 tacattttgaaacatctatagcgataaatgaaacatcttaaaagttttagtatcatattcgt BBa_I761004_sequence 1 atggacaaattcgacgctaatcgccgcaaattgctggcgcttggtggcgttgcactcggtgccgccatcctgccgacccctgcgtttgcaatgcggaacgggctcgtgtggctgctgcaccccgcgctgcccggcaccttgcgctccatcctcggcgcccgcccgccgcccgcgaagcgactgtgtggattcccaaaacagacttacagcacaatgagtaatccggccatccagagaatagaagaccaaattgtcaagtctcctgaagacaaacgggaataccgtggactagagctggccaatggcatcaaagtgcttctcatcagcgatcccaccacagacaagtcctcagcggccctcgatgtgcacataggttcactgtcagaccctccaaatattcctggcttaagtcatttttgtgaacatatgctgtttttgggaaccaagaaatatcctaaagaaaatgaatatagccagtttctcagtgaacatgctggaagttcaaatgcattcaccagtggagaacacaccaattattatttcgatgtttcccatgaacacttggaaggagccctggacaggtttgcgcagtttttcctgtgccccttgtttgatgcaagttgtaaagacagagaggtgaacgctgtcgattcagaacatgagaagaatgtgatgaacgatgcctggagactcttccagctggaaaaggctacggggaaccccaaacaccccttcagcaaatttgggacaggaaacaaatatactctagagactcggcccaaccaagaaggcatcgacgtaagggaagagctcttgaaatttcactctacgtattattcgtccaatctgatggcgatttgtgttttaggtcgagaatccttagacgacctcactaacctggtggtgaagctgttctccgaggtggagaacaagaacgttcctctgcctgagttccctgagcaccctttccaggaagagcatctcaggcaactttataaaatagtacccattaaggatattagaaatctttatgtgacatttcccataccagaccttcagcagtactacaaatccaatcctggtcattatcttggtcatctaattgggcacgaaggtcctggaagcctgttgtcagaactcaaatcaaagggctgggtgaataccctggttggtggacagaaggaaggagcccgaggttttatgttttttatcattaatgtggacttaactgaagaagggttattgcacgttgaagatataattttgcacatgtttcaatacattcagaagctacgtgcagaaggacctcaagaatgggttttccaagagtgcaaggacttgaatgctgttgctttcagatttaaagataaagagagaccacgaggctatacgtccaagattgcaggaaagttgcactattatcccctaaatggagtgctcacagctgaatatctattggaggaatttagacccgacttgatagacatggttcttgataaactcagaccagaaaatgtgcgggtggcaatagtttccaaatcatttgaagggaaaactgaccgcacagagcagtggtatggaacacagtataagcaagaagctatcccagaggacatcattcagaaatggcaaaatgctgaccttaatggaaaatttaagcttccaacaaagaatgaatttattcccacaaattttgagattttgtcattagaaaaagatgcaacaccataccctgctcttattaaggatacagccatgagtaagctgtggttcaaacaagatgataaatttttcttgccgaaagcttgtctcaactttgaatttttcagcccgtttgcttatgtggaccccttgcactgtaacatggcctatttgtaccttgagctcctcaaagactcactcaacgagtatgcatatgcagcagagctagcaggcctgagctatgacctccaaaataccatctatgggatgtatctctcagtgaaaggttacaatgacaaacagccaattttgctgaagaagatcaccgagaaaatggctacttttgagattgataagaagagatttgaaattatcaaagaggcctatatgcgatctcttaataacttccgggctgagcagcctcaccagcatgccatgtactacctccgcttgctgatgactgaagtggcctggactaaagatgagctaaaagaagcccttgacgatgttaccctcccccgacttaaggccttcatacctcagctgctgtcacggctgcatattgaagcacttctccatggaaacataaccaagcaggctgccttaggagttatgcagatggtagaagacactcttatcgagcatgctcacaccaaacctctccttccaagtcagctagtccggtacagagaagtccagctccctgacagaggatggtttgtttatcagcagaggaatgaagttcacaataactgcggcatcgagatctactaccagacagacatgcagagcacctcggagaacatgttcctggagctgttttgccagattatctcggagccttgcttcaataccctgcgcaccaaggagcagcttggctatattgtcttcagtggaccccgtcgggccaacggtatccaaggcttgcgcttcatcatccagtcagaaaaaccaccccactacctggagagcagagtagaagccttcttgattaccatggaaaaggccatagaggacatgacagaggaggcgttccaaaaacacattcaagcactagcgattcggcgactcgacaaaccaaagaaactctctgcggagtgtgctaaatactggggggaaatcatctcgcagcagtacaattacgacagagataacatagaagttgcatatttaaagacacttaccaaggacgatatcatcagattctaccaggaaatgttggctgtcgacgcaccgaggagacataaagtgtctgtccatgttcttgccagggaaatggattcttgtcctgttgttggagagttcccctctcagaatgatataaacttgtccgaagcgccacccttgccacaacctgaagtgattcacaacatgactgagttcaagcgtggcctgccactgtttcccctcgtgaaaccacatattaacttcatggctgcaaaactctgataataa BBa_B0034_sequence 1 aaagaggagaaa BBa_I761006_sequence 1 ccctttgtgcgtccaaacggacgcacggcgctctaaagcgggtcgcgatctttcagattcgctcctcgcgctttcagtctttgttttggcgcatgtcgttatcgcaaaaccgctgcacacttttgcgcgacatgctctgatccccctcatctgggggggcctatctgagggaatttccgatccggctcgcctgaaccattctgctttccacgaacttgaaaacgctactagagtacattttgaaacatctatagcgataaatgaaacatcttaaaagttttagtatcatattcgttactagagaaagaggagaaatactagatggacaaattcgacgctaatcgccgcaaattgctggcgcttggtggcgttgcactcggtgccgccatcctgccgacccctgcgtttgcaatgcggaacgggctcgtgtggctgctgcaccccgcgctgcccggcaccttgcgctccatcctcggcgcccgcccgccgcccgcgaagcgactgtgtggattcccaaaacagacttacagcacaatgagtaatccggccatccagagaatagaagaccaaattgtcaagtctcctgaagacaaacgggaataccgtggactagagctggccaatggcatcaaagtgcttctcatcagcgatcccaccacagacaagtcctcagcggccctcgatgtgcacataggttcactgtcagaccctccaaatattcctggcttaagtcatttttgtgaacatatgctgtttttgggaaccaagaaatatcctaaagaaaatgaatatagccagtttctcagtgaacatgctggaagttcaaatgcattcaccagtggagaacacaccaattattatttcgatgtttcccatgaacacttggaaggagccctggacaggtttgcgcagtttttcctgtgccccttgtttgatgcaagttgtaaagacagagaggtgaacgctgtcgattcagaacatgagaagaatgtgatgaacgatgcctggagactcttccagctggaaaaggctacggggaaccccaaacaccccttcagcaaatttgggacaggaaacaaatatactctagagactcggcccaaccaagaaggcatcgacgtaagggaagagctcttgaaatttcactctacgtattattcgtccaatctgatggcgatttgtgttttaggtcgagaatccttagacgacctcactaacctggtggtgaagctgttctccgaggtggagaacaagaacgttcctctgcctgagttccctgagcaccctttccaggaagagcatctcaggcaactttataaaatagtacccattaaggatattagaaatctttatgtgacatttcccataccagaccttcagcagtactacaaatccaatcctggtcattatcttggtcatctaattgggcacgaaggtcctggaagcctgttgtcagaactcaaatcaaagggctgggtgaataccctggttggtggacagaaggaaggagcccgaggttttatgttttttatcattaatgtggacttaactgaagaagggttattgcacgttgaagatataattttgcacatgtttcaatacattcagaagctacgtgcagaaggacctcaagaatgggttttccaagagtgcaaggacttgaatgctgttgctttcagatttaaagataaagagagaccacgaggctatacgtccaagattgcaggaaagttgcactattatcccctaaatggagtgctcacagctgaatatctattggaggaatttagacccgacttgatagacatggttcttgataaactcagaccagaaaatgtgcgggtggcaatagtttccaaatcatttgaagggaaaactgaccgcacagagcagtggtatggaacacagtataagcaagaagctatcccagaggacatcattcagaaatggcaaaatgctgaccttaatggaaaatttaagcttccaacaaagaatgaatttattcccacaaattttgagattttgtcattagaaaaagatgcaacaccataccctgctcttattaaggatacagccatgagtaagctgtggttcaaacaagatgataaatttttcttgccgaaagcttgtctcaactttgaatttttcagcccgtttgcttatgtggaccccttgcactgtaacatggcctatttgtaccttgagctcctcaaagactcactcaacgagtatgcatatgcagcagagctagcaggcctgagctatgacctccaaaataccatctatgggatgtatctctcagtgaaaggttacaatgacaaacagccaattttgctgaagaagatcaccgagaaaatggctacttttgagattgataagaagagatttgaaattatcaaagaggcctatatgcgatctcttaataacttccgggctgagcagcctcaccagcatgccatgtactacctccgcttgctgatgactgaagtggcctggactaaagatgagctaaaagaagcccttgacgatgttaccctcccccgacttaaggccttcatacctcagctgctgtcacggctgcatattgaagcacttctccatggaaacataaccaagcaggctgccttaggagttatgcagatggtagaagacactcttatcgagcatgctcacaccaaacctctccttccaagtcagctagtccggtacagagaagtccagctccctgacagaggatggtttgtttatcagcagaggaatgaagttcacaataactgcggcatcgagatctactaccagacagacatgcagagcacctcggagaacatgttcctggagctgttttgccagattatctcggagccttgcttcaataccctgcgcaccaaggagcagcttggctatattgtcttcagtggaccccgtcgggccaacggtatccaaggcttgcgcttcatcatccagtcagaaaaaccaccccactacctggagagcagagtagaagccttcttgattaccatggaaaaggccatagaggacatgacagaggaggcgttccaaaaacacattcaagcactagcgattcggcgactcgacaaaccaaagaaactctctgcggagtgtgctaaatactggggggaaatcatctcgcagcagtacaattacgacagagataacatagaagttgcatatttaaagacacttaccaaggacgatatcatcagattctaccaggaaatgttggctgtcgacgcaccgaggagacataaagtgtctgtccatgttcttgccagggaaatggattcttgtcctgttgttggagagttcccctctcagaatgatataaacttgtccgaagcgccacccttgccacaacctgaagtgattcacaacatgactgagttcaagcgtggcctgccactgtttcccctcgtgaaaccacatattaacttcatggctgcaaaactctgataataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z