LOCUS       BBa_I761007              600 bp    DNA     linear       22-Nov-2024
DEFINITION  System 1: glucose controlled human insulin generating device
ACCESSION   BBa_I761007
VERSION     BBa_I761007.1  
FEATURES             Location/Qualifiers
     misc_feature    13..30
                     /label=C1 OmpR
     misc_feature    34..51
                     /label=C2 OmpR
     misc_feature    54..71
                     /label=C3 OmpR
     promoter        75..80
                     /label=-35
     promoter        98..103
                     /label=-10
     promoter        1..108
                     /label=BBa_R0082
     misc_feature    121..124
                     /label=conserved
     RBS             117..128
                     /label=BBa_B0034
     misc_feature    138..222
                     /label=TAT peptide export signal
     CDS             223..285
                     /label=Human insulin A fragment
     CDS             135..285
                     /label=BBa_I761002
     misc_feature    298..301
                     /label=conserved
     RBS             294..305
                     /label=BBa_B0034
     misc_feature    315..399
                     /label=TAT peptide export signal
     CDS             400..491
                     /label=Human insulin B chain
     CDS             312..497
                     /label=BBa_I761003
     stem_loop       513..532
                     /label=T7 TE
     misc_feature    539..539
                     /label=stop
     polyA_site      533..546
                     /label=polya
     misc_feature    506..546
                     /label=BBa_B0012
     terminator      506..546
                     /label=BBa_B0012
     stem_loop       567..589
                     /label=stem_loop
     misc_feature    555..600
                     /label=BBa_B0011
     terminator      555..600
                     /label=BBa_B0011
ORIGIN
        1 tcccttgcat ttacattttg aaacatctat agcgataaat gaaacatctt aaaagtttta
       61 gtatcatatt cgtgttggat tattctgcat ttttggggag aatggactta ctagagaaag
      121 aggagaaata ctagatggac aaattcgacg ctaatcgccg caaattgctg gcgcttggtg
      181 gcgttgcact cggtgccgcc atcctgccga cccctgcgtt tgcaggcatt gtggaacaat
      241 gctgtaccag catctgctcc ctctaccagc tggagaacta ctgcatacta gagaaagagg
      301 agaaatacta gatggacaaa ttcgacgcta atcgccgcaa attgctggcg cttggtggcg
      361 ttgcactcgg tgccgccatc ctgccgaccc ctgcgtttgc atttgtgaac caacacctgt
      421 gcggctcaca cctggtggaa gctctctacc tagtgtgcgg ggaacgaggc ttcttctaca
      481 cacccaagac ctaataatac tagagtcaca ctggctcacc ttcgggtggg cctttctgcg
      541 tttatatact agagagagaa tataaaaagc cagattatta atccggcttt tttattattt
//