LOCUS BBa_I761008 3580 bp DNA linear 22-Nov-2024 DEFINITION System 2: Insulin degradation device ACCESSION BBa_I761008 VERSION BBa_I761008.1 FEATURES Location/Qualifiers misc_feature 1..220 /label=unidentified/uncharacterized CinR dependent stem_loop 35..74 /label=stem_loop stem_loop 91..136 /label=stem_loop promoter 1..225 /label=BBa_R0078 misc_feature 235..252 /label=C1 OmpR misc_feature 255..273 /label=C2 OmpR misc_feature 276..293 /label=C3 OmpR promoter 234..295 /label=BBa_I761001 misc_feature 308..311 /label=conserved RBS 304..315 /label=BBa_B0034 misc_feature 325..409 /label=TAT peptide export signal CDS 410..3471 /label=Mouse insulin degradation enzyme CDS 322..3477 /label=BBa_I761004 stem_loop 3493..3512 /label=T7 TE misc_feature 3519..3519 /label=stop polyA_site 3513..3526 /label=polya misc_feature 3486..3526 /label=BBa_B0012 terminator 3486..3526 /label=BBa_B0012 stem_loop 3547..3569 /label=stem_loop misc_feature 3535..3580 /label=BBa_B0011 terminator 3535..3580 /label=BBa_B0011 ORIGIN 1 ccctttgtgc gtccaaacgg acgcacggcg ctctaaagcg ggtcgcgatc tttcagattc 61 gctcctcgcg ctttcagtct ttgttttggc gcatgtcgtt atcgcaaaac cgctgcacac 121 ttttgcgcga catgctctga tccccctcat ctgggggggc ctatctgagg gaatttccga 181 tccggctcgc ctgaaccatt ctgctttcca cgaacttgaa aacgctacta gagtacattt 241 tgaaacatct atagcgataa atgaaacatc ttaaaagttt tagtatcata ttcgttacta 301 gagaaagagg agaaatacta gatggacaaa ttcgacgcta atcgccgcaa attgctggcg 361 cttggtggcg ttgcactcgg tgccgccatc ctgccgaccc ctgcgtttgc aatgcggaac 421 gggctcgtgt ggctgctgca ccccgcgctg cccggcacct tgcgctccat cctcggcgcc 481 cgcccgccgc ccgcgaagcg actgtgtgga ttcccaaaac agacttacag cacaatgagt 541 aatccggcca tccagagaat agaagaccaa attgtcaagt ctcctgaaga caaacgggaa 601 taccgtggac tagagctggc caatggcatc aaagtgcttc tcatcagcga tcccaccaca 661 gacaagtcct cagcggccct cgatgtgcac ataggttcac tgtcagaccc tccaaatatt 721 cctggcttaa gtcatttttg tgaacatatg ctgtttttgg gaaccaagaa atatcctaaa 781 gaaaatgaat atagccagtt tctcagtgaa catgctggaa gttcaaatgc attcaccagt 841 ggagaacaca ccaattatta tttcgatgtt tcccatgaac acttggaagg agccctggac 901 aggtttgcgc agtttttcct gtgccccttg tttgatgcaa gttgtaaaga cagagaggtg 961 aacgctgtcg attcagaaca tgagaagaat gtgatgaacg atgcctggag actcttccag 1021 ctggaaaagg ctacggggaa ccccaaacac cccttcagca aatttgggac aggaaacaaa 1081 tatactctag agactcggcc caaccaagaa ggcatcgacg taagggaaga gctcttgaaa 1141 tttcactcta cgtattattc gtccaatctg atggcgattt gtgttttagg tcgagaatcc 1201 ttagacgacc tcactaacct ggtggtgaag ctgttctccg aggtggagaa caagaacgtt 1261 cctctgcctg agttccctga gcaccctttc caggaagagc atctcaggca actttataaa 1321 atagtaccca ttaaggatat tagaaatctt tatgtgacat ttcccatacc agaccttcag 1381 cagtactaca aatccaatcc tggtcattat cttggtcatc taattgggca cgaaggtcct 1441 ggaagcctgt tgtcagaact caaatcaaag ggctgggtga ataccctggt tggtggacag 1501 aaggaaggag cccgaggttt tatgtttttt atcattaatg tggacttaac tgaagaaggg 1561 ttattgcacg ttgaagatat aattttgcac atgtttcaat acattcagaa gctacgtgca 1621 gaaggacctc aagaatgggt tttccaagag tgcaaggact tgaatgctgt tgctttcaga 1681 tttaaagata aagagagacc acgaggctat acgtccaaga ttgcaggaaa gttgcactat 1741 tatcccctaa atggagtgct cacagctgaa tatctattgg aggaatttag acccgacttg 1801 atagacatgg ttcttgataa actcagacca gaaaatgtgc gggtggcaat agtttccaaa 1861 tcatttgaag ggaaaactga ccgcacagag cagtggtatg gaacacagta taagcaagaa 1921 gctatcccag aggacatcat tcagaaatgg caaaatgctg accttaatgg aaaatttaag 1981 cttccaacaa agaatgaatt tattcccaca aattttgaga ttttgtcatt agaaaaagat 2041 gcaacaccat accctgctct tattaaggat acagccatga gtaagctgtg gttcaaacaa 2101 gatgataaat ttttcttgcc gaaagcttgt ctcaactttg aatttttcag cccgtttgct 2161 tatgtggacc ccttgcactg taacatggcc tatttgtacc ttgagctcct caaagactca 2221 ctcaacgagt atgcatatgc agcagagcta gcaggcctga gctatgacct ccaaaatacc 2281 atctatggga tgtatctctc agtgaaaggt tacaatgaca aacagccaat tttgctgaag 2341 aagatcaccg agaaaatggc tacttttgag attgataaga agagatttga aattatcaaa 2401 gaggcctata tgcgatctct taataacttc cgggctgagc agcctcacca gcatgccatg 2461 tactacctcc gcttgctgat gactgaagtg gcctggacta aagatgagct aaaagaagcc 2521 cttgacgatg ttaccctccc ccgacttaag gccttcatac ctcagctgct gtcacggctg 2581 catattgaag cacttctcca tggaaacata accaagcagg ctgccttagg agttatgcag 2641 atggtagaag acactcttat cgagcatgct cacaccaaac ctctccttcc aagtcagcta 2701 gtccggtaca gagaagtcca gctccctgac agaggatggt ttgtttatca gcagaggaat 2761 gaagttcaca ataactgcgg catcgagatc tactaccaga cagacatgca gagcacctcg 2821 gagaacatgt tcctggagct gttttgccag attatctcgg agccttgctt caataccctg 2881 cgcaccaagg agcagcttgg ctatattgtc ttcagtggac cccgtcgggc caacggtatc 2941 caaggcttgc gcttcatcat ccagtcagaa aaaccacccc actacctgga gagcagagta 3001 gaagccttct tgattaccat ggaaaaggcc atagaggaca tgacagagga ggcgttccaa 3061 aaacacattc aagcactagc gattcggcga ctcgacaaac caaagaaact ctctgcggag 3121 tgtgctaaat actgggggga aatcatctcg cagcagtaca attacgacag agataacata 3181 gaagttgcat atttaaagac acttaccaag gacgatatca tcagattcta ccaggaaatg 3241 ttggctgtcg acgcaccgag gagacataaa gtgtctgtcc atgttcttgc cagggaaatg 3301 gattcttgtc ctgttgttgg agagttcccc tctcagaatg atataaactt gtccgaagcg 3361 ccacccttgc cacaacctga agtgattcac aacatgactg agttcaagcg tggcctgcca 3421 ctgtttcccc tcgtgaaacc acatattaac ttcatggctg caaaactctg ataataatac 3481 tagagtcaca ctggctcacc ttcgggtggg cctttctgcg tttatatact agagagagaa 3541 tataaaaagc cagattatta atccggcttt tttattattt //