BBa_I761001 1 OmpR_BS OmpR binding site 2007-09-15T11:00:00Z 2015-08-31T04:08:08Z BBa_R0082 OmpR binding site. When position behind a promotor, it will turns that promotor into a OmpR controlled promotor. OmpR will function as an inhibitor of that promotor. false false _140_ 0 2031 9 Not in stock false This part was derived from BBa_R0082 by PCR. false 2007 NYMU_Taiwan iGEM Team annotation1944248 1 C1 OmpR range1944248 1 2 19 annotation1944249 1 C2 OmpR range1944249 1 22 40 annotation1944250 1 C3 OmpR range1944250 1 43 60 BBa_I761008 1 BBa_I761008 System 2: Insulin degradation device 2007-10-18T11:00:00Z 2015-08-31T04:08:08Z *BBa_I761006 *BBa_B0014 This system functions as a negative feedback to degrade extracellular insulin when extracellular glucose concentration is too low. It is essencial because when extracelluar glucose concentration is too low (hypoglycemia), subject death may occur. false false _140_ 0 2031 9 Not in stock false Transcription terminator is added at the end so we can put system 1 and 2 in our project on the same vector. false 2007 NYMU_Taiwan iGEM Team component1950049 1 BBa_I761004 component1950050 1 BBa_B0012 component1950046 1 BBa_B0034 component1950044 1 BBa_I761001 component1950040 1 BBa_R0078 component1950054 1 BBa_B0011 annotation1950054 1 BBa_B0011 range1950054 1 3535 3580 annotation1950046 1 BBa_B0034 range1950046 1 304 315 annotation1950050 1 BBa_B0012 range1950050 1 3486 3526 annotation1950044 1 BBa_I761001 range1950044 1 234 295 annotation1950049 1 BBa_I761004 range1950049 1 322 3477 annotation1950040 1 BBa_R0078 range1950040 1 1 225 BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_I761004 1 BBa_I761004 TAT signal+IDE 2007-10-18T11:00:00Z 2015-08-31T04:08:08Z NCBI accession number: BC041675 K12 E. coli genomic DNA Twin arginin signal peptide is an efficient protein export signal sequence of E. coli. The purpose of this construct is to allow E. coli export mouse insulin degradation enzyme(IDE) automatically, allowing controlled insulin degradation of extracellular insulin. false false _140_ 0 2031 9 Not in stock false To combine TAT signal and mouse IDE, we used two separate PCR reaction to amplify each DNA fragment. We design the reverse primer of TAT signal and the forward primer of mouse IDE to be complement to each other, so when we mixed the product of each PCR reaction together, than used the forward primer of TAT signal and the reverse primer of mouse IDE to conduct PCR reaction, we can isolate the combine DNA product of TAT signal and mouse IDE. (Method devised by Dr. Chang) false 2007 NYMU_Taiwan iGEM Team annotation1949763 1 Mouse insulin degradation enzyme range1949763 1 89 3150 annotation1949762 1 TAT peptide export signal range1949762 1 4 88 BBa_B0011 1 BBa_B0011 LuxICDABEG (+/-) 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from luxICDABEG operon terminator of Vibrio fischeri <genbank>AF170104</genbank>. Released HQ 2013 Bidirectional transcriptional terminator consisting of a 22 bp stem-loop.</p> false false _1_ 0 24 7 In stock false <P> <P>In the naturally-occuring sequence there is a mismatch in the stem of the stem loop. This can be corrected via an A-&gt;G mutation (at position 40 -- sequence coordinate/not MFOLD coordinate). The above sequence does not reflect this mutation (but the MFOLD image does). This terminator's location cannot be found using some inverted repeat detectors like PALINDROME because it is too short and contains a mismatch. This one was found with the help of Tom Knight. It lies between two coding regions that point towards eachother.<P> true Reshma Shetty annotation7019 1 BBa_B0011 range7019 1 1 46 annotation1683 1 stem_loop range1683 1 13 35 BBa_R0078 1 cinR Promoter (cinR and HSL regulated) 2004-01-29T12:00:00Z 2015-05-08T01:14:15Z Rhizobium leguminosarum Released HQ 2013 false false _1_ 0 24 7 In stock false true Drew Endy annotation318234 1 unidentified/uncharacterized CinR dependent range318234 1 1 220 annotation318250 1 stem_loop range318250 1 91 136 annotation318244 1 stem_loop range318244 1 35 74 BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation1690 1 polya range1690 1 28 41 annotation7020 1 BBa_B0012 range7020 1 1 41 annotation1687 1 stop range1687 1 34 34 annotation1686 1 T7 TE range1686 1 8 27 BBa_I761008_sequence 1 ccctttgtgcgtccaaacggacgcacggcgctctaaagcgggtcgcgatctttcagattcgctcctcgcgctttcagtctttgttttggcgcatgtcgttatcgcaaaaccgctgcacacttttgcgcgacatgctctgatccccctcatctgggggggcctatctgagggaatttccgatccggctcgcctgaaccattctgctttccacgaacttgaaaacgctactagagtacattttgaaacatctatagcgataaatgaaacatcttaaaagttttagtatcatattcgttactagagaaagaggagaaatactagatggacaaattcgacgctaatcgccgcaaattgctggcgcttggtggcgttgcactcggtgccgccatcctgccgacccctgcgtttgcaatgcggaacgggctcgtgtggctgctgcaccccgcgctgcccggcaccttgcgctccatcctcggcgcccgcccgccgcccgcgaagcgactgtgtggattcccaaaacagacttacagcacaatgagtaatccggccatccagagaatagaagaccaaattgtcaagtctcctgaagacaaacgggaataccgtggactagagctggccaatggcatcaaagtgcttctcatcagcgatcccaccacagacaagtcctcagcggccctcgatgtgcacataggttcactgtcagaccctccaaatattcctggcttaagtcatttttgtgaacatatgctgtttttgggaaccaagaaatatcctaaagaaaatgaatatagccagtttctcagtgaacatgctggaagttcaaatgcattcaccagtggagaacacaccaattattatttcgatgtttcccatgaacacttggaaggagccctggacaggtttgcgcagtttttcctgtgccccttgtttgatgcaagttgtaaagacagagaggtgaacgctgtcgattcagaacatgagaagaatgtgatgaacgatgcctggagactcttccagctggaaaaggctacggggaaccccaaacaccccttcagcaaatttgggacaggaaacaaatatactctagagactcggcccaaccaagaaggcatcgacgtaagggaagagctcttgaaatttcactctacgtattattcgtccaatctgatggcgatttgtgttttaggtcgagaatccttagacgacctcactaacctggtggtgaagctgttctccgaggtggagaacaagaacgttcctctgcctgagttccctgagcaccctttccaggaagagcatctcaggcaactttataaaatagtacccattaaggatattagaaatctttatgtgacatttcccataccagaccttcagcagtactacaaatccaatcctggtcattatcttggtcatctaattgggcacgaaggtcctggaagcctgttgtcagaactcaaatcaaagggctgggtgaataccctggttggtggacagaaggaaggagcccgaggttttatgttttttatcattaatgtggacttaactgaagaagggttattgcacgttgaagatataattttgcacatgtttcaatacattcagaagctacgtgcagaaggacctcaagaatgggttttccaagagtgcaaggacttgaatgctgttgctttcagatttaaagataaagagagaccacgaggctatacgtccaagattgcaggaaagttgcactattatcccctaaatggagtgctcacagctgaatatctattggaggaatttagacccgacttgatagacatggttcttgataaactcagaccagaaaatgtgcgggtggcaatagtttccaaatcatttgaagggaaaactgaccgcacagagcagtggtatggaacacagtataagcaagaagctatcccagaggacatcattcagaaatggcaaaatgctgaccttaatggaaaatttaagcttccaacaaagaatgaatttattcccacaaattttgagattttgtcattagaaaaagatgcaacaccataccctgctcttattaaggatacagccatgagtaagctgtggttcaaacaagatgataaatttttcttgccgaaagcttgtctcaactttgaatttttcagcccgtttgcttatgtggaccccttgcactgtaacatggcctatttgtaccttgagctcctcaaagactcactcaacgagtatgcatatgcagcagagctagcaggcctgagctatgacctccaaaataccatctatgggatgtatctctcagtgaaaggttacaatgacaaacagccaattttgctgaagaagatcaccgagaaaatggctacttttgagattgataagaagagatttgaaattatcaaagaggcctatatgcgatctcttaataacttccgggctgagcagcctcaccagcatgccatgtactacctccgcttgctgatgactgaagtggcctggactaaagatgagctaaaagaagcccttgacgatgttaccctcccccgacttaaggccttcatacctcagctgctgtcacggctgcatattgaagcacttctccatggaaacataaccaagcaggctgccttaggagttatgcagatggtagaagacactcttatcgagcatgctcacaccaaacctctccttccaagtcagctagtccggtacagagaagtccagctccctgacagaggatggtttgtttatcagcagaggaatgaagttcacaataactgcggcatcgagatctactaccagacagacatgcagagcacctcggagaacatgttcctggagctgttttgccagattatctcggagccttgcttcaataccctgcgcaccaaggagcagcttggctatattgtcttcagtggaccccgtcgggccaacggtatccaaggcttgcgcttcatcatccagtcagaaaaaccaccccactacctggagagcagagtagaagccttcttgattaccatggaaaaggccatagaggacatgacagaggaggcgttccaaaaacacattcaagcactagcgattcggcgactcgacaaaccaaagaaactctctgcggagtgtgctaaatactggggggaaatcatctcgcagcagtacaattacgacagagataacatagaagttgcatatttaaagacacttaccaaggacgatatcatcagattctaccaggaaatgttggctgtcgacgcaccgaggagacataaagtgtctgtccatgttcttgccagggaaatggattcttgtcctgttgttggagagttcccctctcagaatgatataaacttgtccgaagcgccacccttgccacaacctgaagtgattcacaacatgactgagttcaagcgtggcctgccactgtttcccctcgtgaaaccacatattaacttcatggctgcaaaactctgataataatactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattt BBa_R0078_sequence 1 ccctttgtgcgtccaaacggacgcacggcgctctaaagcgggtcgcgatctttcagattcgctcctcgcgctttcagtctttgttttggcgcatgtcgttatcgcaaaaccgctgcacacttttgcgcgacatgctctgatccccctcatctgggggggcctatctgagggaatttccgatccggctcgcctgaaccattctgctttccacgaacttgaaaacgc BBa_I761001_sequence 1 tacattttgaaacatctatagcgataaatgaaacatcttaaaagttttagtatcatattcgt BBa_I761004_sequence 1 atggacaaattcgacgctaatcgccgcaaattgctggcgcttggtggcgttgcactcggtgccgccatcctgccgacccctgcgtttgcaatgcggaacgggctcgtgtggctgctgcaccccgcgctgcccggcaccttgcgctccatcctcggcgcccgcccgccgcccgcgaagcgactgtgtggattcccaaaacagacttacagcacaatgagtaatccggccatccagagaatagaagaccaaattgtcaagtctcctgaagacaaacgggaataccgtggactagagctggccaatggcatcaaagtgcttctcatcagcgatcccaccacagacaagtcctcagcggccctcgatgtgcacataggttcactgtcagaccctccaaatattcctggcttaagtcatttttgtgaacatatgctgtttttgggaaccaagaaatatcctaaagaaaatgaatatagccagtttctcagtgaacatgctggaagttcaaatgcattcaccagtggagaacacaccaattattatttcgatgtttcccatgaacacttggaaggagccctggacaggtttgcgcagtttttcctgtgccccttgtttgatgcaagttgtaaagacagagaggtgaacgctgtcgattcagaacatgagaagaatgtgatgaacgatgcctggagactcttccagctggaaaaggctacggggaaccccaaacaccccttcagcaaatttgggacaggaaacaaatatactctagagactcggcccaaccaagaaggcatcgacgtaagggaagagctcttgaaatttcactctacgtattattcgtccaatctgatggcgatttgtgttttaggtcgagaatccttagacgacctcactaacctggtggtgaagctgttctccgaggtggagaacaagaacgttcctctgcctgagttccctgagcaccctttccaggaagagcatctcaggcaactttataaaatagtacccattaaggatattagaaatctttatgtgacatttcccataccagaccttcagcagtactacaaatccaatcctggtcattatcttggtcatctaattgggcacgaaggtcctggaagcctgttgtcagaactcaaatcaaagggctgggtgaataccctggttggtggacagaaggaaggagcccgaggttttatgttttttatcattaatgtggacttaactgaagaagggttattgcacgttgaagatataattttgcacatgtttcaatacattcagaagctacgtgcagaaggacctcaagaatgggttttccaagagtgcaaggacttgaatgctgttgctttcagatttaaagataaagagagaccacgaggctatacgtccaagattgcaggaaagttgcactattatcccctaaatggagtgctcacagctgaatatctattggaggaatttagacccgacttgatagacatggttcttgataaactcagaccagaaaatgtgcgggtggcaatagtttccaaatcatttgaagggaaaactgaccgcacagagcagtggtatggaacacagtataagcaagaagctatcccagaggacatcattcagaaatggcaaaatgctgaccttaatggaaaatttaagcttccaacaaagaatgaatttattcccacaaattttgagattttgtcattagaaaaagatgcaacaccataccctgctcttattaaggatacagccatgagtaagctgtggttcaaacaagatgataaatttttcttgccgaaagcttgtctcaactttgaatttttcagcccgtttgcttatgtggaccccttgcactgtaacatggcctatttgtaccttgagctcctcaaagactcactcaacgagtatgcatatgcagcagagctagcaggcctgagctatgacctccaaaataccatctatgggatgtatctctcagtgaaaggttacaatgacaaacagccaattttgctgaagaagatcaccgagaaaatggctacttttgagattgataagaagagatttgaaattatcaaagaggcctatatgcgatctcttaataacttccgggctgagcagcctcaccagcatgccatgtactacctccgcttgctgatgactgaagtggcctggactaaagatgagctaaaagaagcccttgacgatgttaccctcccccgacttaaggccttcatacctcagctgctgtcacggctgcatattgaagcacttctccatggaaacataaccaagcaggctgccttaggagttatgcagatggtagaagacactcttatcgagcatgctcacaccaaacctctccttccaagtcagctagtccggtacagagaagtccagctccctgacagaggatggtttgtttatcagcagaggaatgaagttcacaataactgcggcatcgagatctactaccagacagacatgcagagcacctcggagaacatgttcctggagctgttttgccagattatctcggagccttgcttcaataccctgcgcaccaaggagcagcttggctatattgtcttcagtggaccccgtcgggccaacggtatccaaggcttgcgcttcatcatccagtcagaaaaaccaccccactacctggagagcagagtagaagccttcttgattaccatggaaaaggccatagaggacatgacagaggaggcgttccaaaaacacattcaagcactagcgattcggcgactcgacaaaccaaagaaactctctgcggagtgtgctaaatactggggggaaatcatctcgcagcagtacaattacgacagagataacatagaagttgcatatttaaagacacttaccaaggacgatatcatcagattctaccaggaaatgttggctgtcgacgcaccgaggagacataaagtgtctgtccatgttcttgccagggaaatggattcttgtcctgttgttggagagttcccctctcagaatgatataaacttgtccgaagcgccacccttgccacaacctgaagtgattcacaacatgactgagttcaagcgtggcctgccactgtttcccctcgtgaaaccacatattaacttcatggctgcaaaactctgataataa BBa_B0034_sequence 1 aaagaggagaaa BBa_B0011_sequence 1 agagaatataaaaagccagattattaatccggcttttttattattt BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z