BBa_I761008
type: ComponentDefinition
persistent identity: BBa I761008
display id: BBa_I761008
version: 1
was generated by: iGEM to SBOL conversion
description: System 2: Insulin degradation device
created: 2007-10-18T11:00:00Z
modified: 2015-08-31T04:08:08Z
mutable provenance: *BBa_I761006 *BBa_B0014
owned by: james
owned by: myers
mutable description: This system functions as a negative feedback to degrade extracellular insulin when extracellular glucose concentration is too low. It is essencial because when extracelluar glucose concentration is too low (hypoglycemia), subject death may occur.
discontinued: false
dominant: false
experience: experience/None
group u list: _140_
m user id: 0
owner id: 2031
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: Transcription terminator is added at the end so we can put system 1 and 2 in our project on the same vector.
star: false
creator: 2007 NYMU_Taiwan iGEM Team
type: DnaRegion
role: SO:0000804
role: partType/Project
component: BBa_I761001
component: BBa_R0078
component: BBa_I761004
component: BBa_B0034
component: BBa_B0011
component: BBa_B0012
sequence annotation: BBa_R0078
sequence annotation: BBa_I761001
sequence annotation: BBa_B0034
sequence annotation: BBa_I761004
sequence annotation: BBa_B0012
sequence annotation: BBa_B0011
sequence: https://synbiohub.org/public/igem/BBa_I761008_sequence/1

BBa_I761001
type: Component
persistent identity: component1950044
display id: component1950044
version: 1
top level: BBa_I761008
definition: OmpR_BS
access: public

BBa_R0078
type: Component
persistent identity: component1950040
display id: component1950040
version: 1
top level: BBa_I761008
definition: cinR
access: public

BBa_I761004
type: Component
persistent identity: component1950049
display id: component1950049
version: 1
top level: BBa_I761008
definition: BBa_I761004
access: public

BBa_B0034
type: Component
persistent identity: component1950046
display id: component1950046
version: 1
top level: BBa_I761008
definition: BBa_B0034
access: public

BBa_B0011
type: Component
persistent identity: component1950054
display id: component1950054
version: 1
top level: BBa_I761008
definition: BBa_B0011
access: public

BBa_B0012
type: Component
persistent identity: component1950050
display id: component1950050
version: 1
top level: BBa_I761008
definition: BBa_B0012
access: public

BBa_R0078
type: SequenceAnnotation
persistent identity: annotation1950040
display id: annotation1950040
version: 1
top level: BBa_I761008
location: https://synbiohub.org/public/igem/BBa_I761008/annotation1950040/range1950040/1
component: BBa_R0078

https://synbiohub.org/public/igem/BBa_I761008/annotation1950040/range1950040/1
type: Range
persistent identity: range1950040
display id: range1950040
version: 1
top level: BBa_I761008
direction: direction/reverse
start: 1
end: 225
orientation: inline

BBa_I761001
type: SequenceAnnotation
persistent identity: annotation1950044
display id: annotation1950044
version: 1
top level: BBa_I761008
location: https://synbiohub.org/public/igem/BBa_I761008/annotation1950044/range1950044/1
component: BBa_I761001

https://synbiohub.org/public/igem/BBa_I761008/annotation1950044/range1950044/1
type: Range
persistent identity: range1950044
display id: range1950044
version: 1
top level: BBa_I761008
direction: direction/reverse
start: 234
end: 295
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1950046
display id: annotation1950046
version: 1
top level: BBa_I761008
location: https://synbiohub.org/public/igem/BBa_I761008/annotation1950046/range1950046/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I761008/annotation1950046/range1950046/1
type: Range
persistent identity: range1950046
display id: range1950046
version: 1
top level: BBa_I761008
direction: direction/reverse
start: 304
end: 315
orientation: inline

BBa_I761004
type: SequenceAnnotation
persistent identity: annotation1950049
display id: annotation1950049
version: 1
top level: BBa_I761008
location: https://synbiohub.org/public/igem/BBa_I761008/annotation1950049/range1950049/1
component: BBa_I761004

https://synbiohub.org/public/igem/BBa_I761008/annotation1950049/range1950049/1
type: Range
persistent identity: range1950049
display id: range1950049
version: 1
top level: BBa_I761008
direction: direction/reverse
start: 322
end: 3477
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1950050
display id: annotation1950050
version: 1
top level: BBa_I761008
location: https://synbiohub.org/public/igem/BBa_I761008/annotation1950050/range1950050/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I761008/annotation1950050/range1950050/1
type: Range
persistent identity: range1950050
display id: range1950050
version: 1
top level: BBa_I761008
direction: direction/forward
start: 3486
end: 3526
orientation: inline

BBa_B0011
type: SequenceAnnotation
persistent identity: annotation1950054
display id: annotation1950054
version: 1
top level: BBa_I761008
location: https://synbiohub.org/public/igem/BBa_I761008/annotation1950054/range1950054/1
component: BBa_B0011

https://synbiohub.org/public/igem/BBa_I761008/annotation1950054/range1950054/1
type: Range
persistent identity: range1950054
display id: range1950054
version: 1
top level: BBa_I761008
direction: direction/forward
start: 3535
end: 3580
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

OmpR_BS
type: ComponentDefinition
persistent identity: BBa I761001
display id: BBa_I761001
version: 1
was generated by: iGEM to SBOL conversion
description: OmpR binding site
created: 2007-09-15T11:00:00Z
modified: 2015-08-31T04:08:08Z
mutable provenance: BBa_R0082
owned by: james
owned by: myers
mutable description: OmpR binding site. When position behind a promotor, it will turns that promotor into a OmpR controlled promotor. OmpR will function as an inhibitor of that promotor.
discontinued: false
dominant: false
experience: experience/None
group u list: _140_
m user id: 0
owner id: 2031
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: This part was derived from BBa_R0082 by PCR.
star: false
creator: 2007 NYMU_Taiwan iGEM Team
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: C1 OmpR
sequence annotation: C2 OmpR
sequence annotation: C3 OmpR
sequence: https://synbiohub.org/public/igem/BBa_I761001_sequence/1

C1 OmpR
type: SequenceAnnotation
persistent identity: annotation1944248
display id: annotation1944248
version: 1
top level: OmpR_BS
location: https://synbiohub.org/public/igem/BBa_I761001/annotation1944248/range1944248/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_I761001/annotation1944248/range1944248/1
type: Range
persistent identity: range1944248
display id: range1944248
version: 1
top level: OmpR_BS
direction: direction/forward
start: 2
end: 19
orientation: inline

C2 OmpR
type: SequenceAnnotation
persistent identity: annotation1944249
display id: annotation1944249
version: 1
top level: OmpR_BS
location: https://synbiohub.org/public/igem/BBa_I761001/annotation1944249/range1944249/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_I761001/annotation1944249/range1944249/1
type: Range
persistent identity: range1944249
display id: range1944249
version: 1
top level: OmpR_BS
direction: direction/forward
start: 22
end: 40
orientation: inline

C3 OmpR
type: SequenceAnnotation
persistent identity: annotation1944250
display id: annotation1944250
version: 1
top level: OmpR_BS
location: https://synbiohub.org/public/igem/BBa_I761001/annotation1944250/range1944250/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_I761001/annotation1944250/range1944250/1
type: Range
persistent identity: range1944250
display id: range1944250
version: 1
top level: OmpR_BS
direction: direction/forward
start: 43
end: 60
orientation: inline

BBa_I761004
type: ComponentDefinition
persistent identity: BBa I761004
display id: BBa_I761004
version: 1
was generated by: iGEM to SBOL conversion
description: TAT signal+IDE
created: 2007-10-18T11:00:00Z
modified: 2015-08-31T04:08:08Z
mutable provenance: NCBI accession number: BC041675 K12 E. coli genomic DNA
owned by: james
owned by: myers
mutable description: Twin arginin signal peptide is an efficient protein export signal sequence of E. coli. The purpose of this construct is to allow E. coli export mouse insulin degradation enzyme(IDE) automatically, allowing controlled insulin degradation of extracellular insulin.
discontinued: false
dominant: false
experience: experience/None
group u list: _140_
m user id: 0
owner id: 2031
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: To combine TAT signal and mouse IDE, we used two separate PCR reaction to amplify each DNA fragment. We design the reverse primer of TAT signal and the forward primer of mouse IDE to be complement to each other, so when we mixed the product of each PCR reaction together, than used the forward primer of TAT signal and the reverse primer of mouse IDE to conduct PCR reaction, we can isolate the combine DNA product of TAT signal and mouse IDE. (Method devised by Dr. Chang)
star: false
creator: 2007 NYMU_Taiwan iGEM Team
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: TAT peptide export signal
sequence annotation: Mouse insulin degradation enzyme
sequence: https://synbiohub.org/public/igem/BBa_I761004_sequence/1

TAT peptide export signal
type: SequenceAnnotation
persistent identity: annotation1949762
display id: annotation1949762
version: 1
top level: BBa_I761004
location: https://synbiohub.org/public/igem/BBa_I761004/annotation1949762/range1949762/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_I761004/annotation1949762/range1949762/1
type: Range
persistent identity: range1949762
display id: range1949762
version: 1
top level: BBa_I761004
direction: direction/forward
start: 4
end: 88
orientation: inline

Mouse insulin degradation enzyme
type: SequenceAnnotation
persistent identity: annotation1949763
display id: annotation1949763
version: 1
top level: BBa_I761004
location: https://synbiohub.org/public/igem/BBa_I761004/annotation1949763/range1949763/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_I761004/annotation1949763/range1949763/1
type: Range
persistent identity: range1949763
display id: range1949763
version: 1
top level: BBa_I761004
direction: direction/forward
start: 89
end: 3150
orientation: inline

cinR
type: ComponentDefinition
persistent identity: BBa R0078
display id: BBa_R0078
version: 1
was generated by: iGEM to SBOL conversion
description: Promoter (cinR and HSL regulated)
created: 2004-01-29T12:00:00Z
modified: 2015-05-08T01:14:15Z
mutable provenance: Rhizobium leguminosarum
owned by: james
owned by: myers
part status: Released HQ 2013
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Drew Endy
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: unidentified/uncharacterized CinR dependent
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_R0078_sequence/1

unidentified/uncharacterized CinR dependent
type: SequenceAnnotation
persistent identity: annotation318234
display id: annotation318234
version: 1
top level: cinR
location: https://synbiohub.org/public/igem/BBa_R0078/annotation318234/range318234/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_R0078/annotation318234/range318234/1
type: Range
persistent identity: range318234
display id: range318234
version: 1
top level: cinR
direction: direction/forward
start: 1
end: 220
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation318244
display id: annotation318244
version: 1
top level: cinR
location: https://synbiohub.org/public/igem/BBa_R0078/annotation318244/range318244/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_R0078/annotation318244/range318244/1
type: Range
persistent identity: range318244
display id: range318244
version: 1
top level: cinR
direction: direction/forward
start: 35
end: 74
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation318250
display id: annotation318250
version: 1
top level: cinR
location: https://synbiohub.org/public/igem/BBa_R0078/annotation318250/range318250/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_R0078/annotation318250/range318250/1
type: Range
persistent identity: range318250
display id: range318250
version: 1
top level: cinR
direction: direction/forward
start: 91
end: 136
orientation: inline

BBa_B0011
type: ComponentDefinition
persistent identity: BBa B0011
display id: BBa_B0011
version: 1
was generated by: iGEM to SBOL conversion
description: LuxICDABEG (+/-)
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from luxICDABEG operon terminator of Vibrio fischeri <genbank>AF170104</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Bidirectional transcriptional terminator consisting of a 22 bp stem-loop.
discontinued: false
dominant: false
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: In the naturally-occuring sequence there is a mismatch in the stem of the stem loop. This can be corrected via an A->G mutation (at position 40 -- sequence coordinate/not MFOLD coordinate). The above sequence does not reflect this mutation (but the MFOLD image does). This terminator's location cannot be found using some inverted repeat detectors like PALINDROME because it is too short and contains a mismatch. This one was found with the help of Tom Knight. It lies between two coding regions that point towards eachother.
star: true
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0011
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0011_sequence/1

BBa_B0011
type: SequenceAnnotation
persistent identity: annotation7019
display id: annotation7019
version: 1
top level: BBa_B0011
location: https://synbiohub.org/public/igem/BBa_B0011/annotation7019/range7019/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0011/annotation7019/range7019/1
type: Range
persistent identity: range7019
display id: range7019
version: 1
top level: BBa_B0011
direction: direction/forward
start: 1
end: 46
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation1683
display id: annotation1683
version: 1
top level: BBa_B0011
location: https://synbiohub.org/public/igem/BBa_B0011/annotation1683/range1683/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0011/annotation1683/range1683/1
type: Range
persistent identity: range1683
display id: range1683
version: 1
top level: BBa_B0011
direction: direction/forward
start: 13
end: 35
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

https://synbiohub.org/public/igem/BBa_I761008_sequence/1
type: Sequence
persistent identity: BBa I761008 sequence
display id: BBa_I761008_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccctttgtgcgtccaaacggacgcacggcgctctaaagcgggtcgcgatctttcagattcgctcctcgcgctttcagtctttgttttggcgcatgtcgttatcgcaaaaccgctgcacacttttgcgcgacatgctctgatccccctcatctgggggggcctatctgagggaatttccgatccggctcgcctgaaccattctgctttccacgaacttgaaaacgctactagagtacattttgaaacatctatagcgataaatgaaacatcttaaaagttttagtatcatattcgttactagagaaagaggagaaatactagatggacaaattcgacgctaatcgccgcaaattgctggcgcttggtggcgttgcactcggtgccgccatcctgccgacccctgcgtttgcaatgcggaacgggctcgtgtggctgctgcaccccgcgctgcccggcaccttgcgctccatcctcggcgcccgcccgccgcccgcgaagcgactgtgtggattcccaaaacagacttacagcacaatgagtaatccggccatccagagaatagaagaccaaattgtcaagtctcctgaagacaaacgggaataccgtggactagagctggccaatggcatcaaagtgcttctcatcagcgatcccaccacagacaagtcctcagcggccctcgatgtgcacataggttcactgtcagaccctccaaatattcctggcttaagtcatttttgtgaacatatgctgtttttgggaaccaagaaatatcctaaagaaaatgaatatagccagtttctcagtgaacatgctggaagttcaaatgcattcaccagtggagaacacaccaattattatttcgatgtttcccatgaacacttggaaggagccctggacaggtttgcgcagtttttcctgtgccccttgtttgatgcaagttgtaaagacagagaggtgaacgctgtcgattcagaacatgagaagaatgtgatgaacgatgcctggagactcttccagctggaaaaggctacggggaaccccaaacaccccttcagcaaatttgggacaggaaacaaatatactctagagactcggcccaaccaagaaggcatcgacgtaagggaagagctcttgaaatttcactctacgtattattcgtccaatctgatggcgatttgtgttttaggtcgagaatccttagacgacctcactaacctggtggtgaagctgttctccgaggtggagaacaagaacgttcctctgcctgagttccctgagcaccctttccaggaagagcatctcaggcaactttataaaatagtacccattaaggatattagaaatctttatgtgacatttcccataccagaccttcagcagtactacaaatccaatcctggtcattatcttggtcatctaattgggcacgaaggtcctggaagcctgttgtcagaactcaaatcaaagggctgggtgaataccctggttggtggacagaaggaaggagcccgaggttttatgttttttatcattaatgtggacttaactgaagaagggttattgcacgttgaagatataattttgcacatgtttcaatacattcagaagctacgtgcagaaggacctcaagaatgggttttccaagagtgcaaggacttgaatgctgttgctttcagatttaaagataaagagagaccacgaggctatacgtccaagattgcaggaaagttgcactattatcccctaaatggagtgctcacagctgaatatctattggaggaatttagacccgacttgatagacatggttcttgataaactcagaccagaaaatgtgcgggtggcaatagtttccaaatcatttgaagggaaaactgaccgcacagagcagtggtatggaacacagtataagcaagaagctatcccagaggacatcattcagaaatggcaaaatgctgaccttaatggaaaatttaagcttccaacaaagaatgaatt…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I761001_sequence/1
type: Sequence
persistent identity: BBa I761001 sequence
display id: BBa_I761001_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tacattttgaaacatctatagcgataaatgaaacatcttaaaagttttagtatcatattcgt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I761004_sequence/1
type: Sequence
persistent identity: BBa I761004 sequence
display id: BBa_I761004_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggacaaattcgacgctaatcgccgcaaattgctggcgcttggtggcgttgcactcggtgccgccatcctgccgacccctgcgtttgcaatgcggaacgggctcgtgtggctgctgcaccccgcgctgcccggcaccttgcgctccatcctcggcgcccgcccgccgcccgcgaagcgactgtgtggattcccaaaacagacttacagcacaatgagtaatccggccatccagagaatagaagaccaaattgtcaagtctcctgaagacaaacgggaataccgtggactagagctggccaatggcatcaaagtgcttctcatcagcgatcccaccacagacaagtcctcagcggccctcgatgtgcacataggttcactgtcagaccctccaaatattcctggcttaagtcatttttgtgaacatatgctgtttttgggaaccaagaaatatcctaaagaaaatgaatatagccagtttctcagtgaacatgctggaagttcaaatgcattcaccagtggagaacacaccaattattatttcgatgtttcccatgaacacttggaaggagccctggacaggtttgcgcagtttttcctgtgccccttgtttgatgcaagttgtaaagacagagaggtgaacgctgtcgattcagaacatgagaagaatgtgatgaacgatgcctggagactcttccagctggaaaaggctacggggaaccccaaacaccccttcagcaaatttgggacaggaaacaaatatactctagagactcggcccaaccaagaaggcatcgacgtaagggaagagctcttgaaatttcactctacgtattattcgtccaatctgatggcgatttgtgttttaggtcgagaatccttagacgacctcactaacctggtggtgaagctgttctccgaggtggagaacaagaacgttcctctgcctgagttccctgagcaccctttccaggaagagcatctcaggcaactttataaaatagtacccattaaggatattagaaatctttatgtgacatttcccataccagaccttcagcagtactacaaatccaatcctggtcattatcttggtcatctaattgggcacgaaggtcctggaagcctgttgtcagaactcaaatcaaagggctgggtgaataccctggttggtggacagaaggaaggagcccgaggttttatgttttttatcattaatgtggacttaactgaagaagggttattgcacgttgaagatataattttgcacatgtttcaatacattcagaagctacgtgcagaaggacctcaagaatgggttttccaagagtgcaaggacttgaatgctgttgctttcagatttaaagataaagagagaccacgaggctatacgtccaagattgcaggaaagttgcactattatcccctaaatggagtgctcacagctgaatatctattggaggaatttagacccgacttgatagacatggttcttgataaactcagaccagaaaatgtgcgggtggcaatagtttccaaatcatttgaagggaaaactgaccgcacagagcagtggtatggaacacagtataagcaagaagctatcccagaggacatcattcagaaatggcaaaatgctgaccttaatggaaaatttaagcttccaacaaagaatgaatttattcccacaaattttgagattttgtcattagaaaaagatgcaacaccataccctgctcttattaaggatacagccatgagtaagctgtggttcaaacaagatgataaatttttcttgccgaaagcttgtctcaactttgaatttttcagcccgtttgcttatgtggaccccttgcactgtaacatggcctatttgtaccttgagctcctcaaagactcactcaacgagtatgcatatgcagcagagctagcaggcctgagctatgacctccaaaataccatctatgggatgtatctctcagtgaaaggttacaatgacaa…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0078_sequence/1
type: Sequence
persistent identity: BBa R0078 sequence
display id: BBa_R0078_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccctttgtgcgtccaaacggacgcacggcgctctaaagcgggtcgcgatctttcagattcgctcctcgcgctttcagtctttgttttggcgcatgtcgttatcgcaaaaccgctgcacacttttgcgcgacatgctctgatccccctcatctgggggggcctatctgagggaatttccgatccggctcgcctgaaccattctgctttccacgaacttgaaaacgc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0011_sequence/1
type: Sequence
persistent identity: BBa B0011 sequence
display id: BBa_B0011_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: agagaatataaaaagccagattattaatccggcttttttattattt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z