BBa_R0078 1 cinR Promoter (cinR and HSL regulated) 2004-01-29T12:00:00Z 2015-05-08T01:14:15Z Rhizobium leguminosarum Released HQ 2013 false false _1_ 0 24 7 In stock false true Drew Endy annotation318234 1 unidentified/uncharacterized CinR dependent range318234 1 1 220 annotation318244 1 stem_loop range318244 1 35 74 annotation318250 1 stem_loop range318250 1 91 136 BBa_I761002 1 BBa_I761002 TAT signal+INS_A 2007-10-18T11:00:00Z 2015-08-31T04:08:08Z NCBI accession number: BC005255 K12 E. coli genomic DNA Twin arginin signal peptide is an efficient protein export signal sequence of E. coli. The purpose of this construct is to allow E. coli export insulin automatically, obviate the need to extract insulin by biochemical method. false false _140_ 0 2031 9 Not in stock false To combine TAT signal and INS_A, we used two separate PCR reaction to amplify each DNA fragment. We design the reverse primer of TAT signal and the forward primer of INS_A to be complement to each other, so when we mixed the product of each PCR reaction together, than used the forward primer of TAT signal and the reverse primer of INS_A to conduct PCR reaction, we can isolate the combine DNA product of TAT signal and INS_A. (Method devised by Dr. Chang) false 2007 NYMU_Taiwan iGEM Team annotation1949759 1 Human insulin A fragment range1949759 1 89 151 annotation1949758 1 TAT peptide export signal range1949758 1 4 88 BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation1690 1 polya range1690 1 28 41 annotation1686 1 T7 TE range1686 1 8 27 annotation1687 1 stop range1687 1 34 34 annotation7020 1 BBa_B0012 range7020 1 1 41 BBa_I761009 1 BBa_I761009 Complete system: GlucOperon 2007-10-18T11:00:00Z 2015-08-31T04:08:08Z *BBa_I761007 *BBa_I761008 GlucOperon, the next generation therapeutics for diabetes mellitus patient. false false _140_ 0 2031 9 Not in stock false Combined insulin generating device and insulin degradation device on the same vector. false 2007 NYMU_Taiwan iGEM Team component1950091 1 BBa_B0012 component1950066 1 BBa_I761002 component1950090 1 BBa_I761004 component1950081 1 BBa_R0078 component1950085 1 BBa_I761001 component1950076 1 BBa_B0011 component1950087 1 BBa_B0034 component1950063 1 BBa_B0034 component1950072 1 BBa_B0012 component1950068 1 BBa_B0034 component1950061 1 BBa_R0082 component1950071 1 BBa_I761003 component1950095 1 BBa_B0011 annotation1950071 1 BBa_I761003 range1950071 1 312 497 annotation1950063 1 BBa_B0034 range1950063 1 117 128 annotation1950095 1 BBa_B0011 range1950095 1 4143 4188 annotation1950066 1 BBa_I761002 range1950066 1 135 285 annotation1950091 1 BBa_B0012 range1950091 1 4094 4134 annotation1950072 1 BBa_B0012 range1950072 1 506 546 annotation1950076 1 BBa_B0011 range1950076 1 555 600 annotation1950081 1 BBa_R0078 range1950081 1 609 833 annotation1950061 1 BBa_R0082 range1950061 1 1 108 annotation1950090 1 BBa_I761004 range1950090 1 930 4085 annotation1950087 1 BBa_B0034 range1950087 1 912 923 annotation1950068 1 BBa_B0034 range1950068 1 294 305 annotation1950085 1 BBa_I761001 range1950085 1 842 903 BBa_R0082 1 OmpR Promoter (OmpR, positive) 2004-01-27T12:00:00Z 2015-05-08T01:14:15Z NC_000193 E. coli K12 Released HQ 2013 Positively regulated, OmpR-controlled promoter. This promoter is taken from the upstream region of ompC. Phosphorylated OmpR binds to the three operator sites and activates transcription. false false _1_ 0 24 7 In stock false The promoter includes the three OmpR binding sites: C1, C2, and C3. The promoter starts at -107 and goes up to the transcriptional start site at +1. An alternate version, BBa_R0083, cuts out the C2 and C3 sites. The efficacy of this promoter versus the truncated ompC upstream region (BBa_R0083) or the ompF upstream region (BBa_R0084) is currently unknown. true Stephen Lee, Roshan Kumar, Joe Levine, Ziyan Chu (Polkadorks, IAP 2004) annotation301156 1 C3 OmpR range301156 1 54 71 annotation301154 1 C1 OmpR range301154 1 13 30 annotation301167 1 -10 range301167 1 98 103 annotation301155 1 C2 OmpR range301155 1 34 51 annotation301166 1 -35 range301166 1 75 80 BBa_I761003 1 BBa_I761003 TAT signal+INS_B 2007-10-18T11:00:00Z 2015-08-31T04:08:08Z NCBI accession number: BC005255 K12 E. coli genomic DNA Twin arginin signal peptide is an efficient protein export signal sequence of E. coli. The purpose of this construct is to allow E. coli export insulin automatically, obviate the need to extract insulin by biochemical method. false false _140_ 0 2031 9 Not in stock false To combine TAT signal and INS_B, we used two separate PCR reaction to amplify each DNA fragment. We design the reverse primer of TAT signal and the forward primer of INS_B to be complement to each other, so when we mixed the product of each PCR reaction together, than used the forward primer of TAT signal and the reverse primer of INS_B to conduct PCR reaction, we can isolate the combine DNA product of TAT signal and INS_B. (Method devised by Dr. Chang) false 2007 NYMU_Taiwan iGEM Team annotation1949760 1 TAT peptide export signal range1949760 1 4 88 annotation1949761 1 Human insulin B chain range1949761 1 89 180 BBa_I761001 1 OmpR_BS OmpR binding site 2007-09-15T11:00:00Z 2015-08-31T04:08:08Z BBa_R0082 OmpR binding site. When position behind a promotor, it will turns that promotor into a OmpR controlled promotor. OmpR will function as an inhibitor of that promotor. false false _140_ 0 2031 9 Not in stock false This part was derived from BBa_R0082 by PCR. false 2007 NYMU_Taiwan iGEM Team annotation1944249 1 C2 OmpR range1944249 1 22 40 annotation1944250 1 C3 OmpR range1944250 1 43 60 annotation1944248 1 C1 OmpR range1944248 1 2 19 BBa_I761004 1 BBa_I761004 TAT signal+IDE 2007-10-18T11:00:00Z 2015-08-31T04:08:08Z NCBI accession number: BC041675 K12 E. coli genomic DNA Twin arginin signal peptide is an efficient protein export signal sequence of E. coli. The purpose of this construct is to allow E. coli export mouse insulin degradation enzyme(IDE) automatically, allowing controlled insulin degradation of extracellular insulin. false false _140_ 0 2031 9 Not in stock false To combine TAT signal and mouse IDE, we used two separate PCR reaction to amplify each DNA fragment. We design the reverse primer of TAT signal and the forward primer of mouse IDE to be complement to each other, so when we mixed the product of each PCR reaction together, than used the forward primer of TAT signal and the reverse primer of mouse IDE to conduct PCR reaction, we can isolate the combine DNA product of TAT signal and mouse IDE. (Method devised by Dr. Chang) false 2007 NYMU_Taiwan iGEM Team annotation1949763 1 Mouse insulin degradation enzyme range1949763 1 89 3150 annotation1949762 1 TAT peptide export signal range1949762 1 4 88 BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_B0011 1 BBa_B0011 LuxICDABEG (+/-) 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from luxICDABEG operon terminator of Vibrio fischeri <genbank>AF170104</genbank>. Released HQ 2013 Bidirectional transcriptional terminator consisting of a 22 bp stem-loop.</p> false false _1_ 0 24 7 In stock false <P> <P>In the naturally-occuring sequence there is a mismatch in the stem of the stem loop. This can be corrected via an A-&gt;G mutation (at position 40 -- sequence coordinate/not MFOLD coordinate). The above sequence does not reflect this mutation (but the MFOLD image does). This terminator's location cannot be found using some inverted repeat detectors like PALINDROME because it is too short and contains a mismatch. This one was found with the help of Tom Knight. It lies between two coding regions that point towards eachother.<P> true Reshma Shetty annotation7019 1 BBa_B0011 range7019 1 1 46 annotation1683 1 stem_loop range1683 1 13 35 BBa_R0078_sequence 1 ccctttgtgcgtccaaacggacgcacggcgctctaaagcgggtcgcgatctttcagattcgctcctcgcgctttcagtctttgttttggcgcatgtcgttatcgcaaaaccgctgcacacttttgcgcgacatgctctgatccccctcatctgggggggcctatctgagggaatttccgatccggctcgcctgaaccattctgctttccacgaacttgaaaacgc BBa_R0082_sequence 1 tcccttgcatttacattttgaaacatctatagcgataaatgaaacatcttaaaagttttagtatcatattcgtgttggattattctgcatttttggggagaatggact BBa_I761001_sequence 1 tacattttgaaacatctatagcgataaatgaaacatcttaaaagttttagtatcatattcgt BBa_I761004_sequence 1 atggacaaattcgacgctaatcgccgcaaattgctggcgcttggtggcgttgcactcggtgccgccatcctgccgacccctgcgtttgcaatgcggaacgggctcgtgtggctgctgcaccccgcgctgcccggcaccttgcgctccatcctcggcgcccgcccgccgcccgcgaagcgactgtgtggattcccaaaacagacttacagcacaatgagtaatccggccatccagagaatagaagaccaaattgtcaagtctcctgaagacaaacgggaataccgtggactagagctggccaatggcatcaaagtgcttctcatcagcgatcccaccacagacaagtcctcagcggccctcgatgtgcacataggttcactgtcagaccctccaaatattcctggcttaagtcatttttgtgaacatatgctgtttttgggaaccaagaaatatcctaaagaaaatgaatatagccagtttctcagtgaacatgctggaagttcaaatgcattcaccagtggagaacacaccaattattatttcgatgtttcccatgaacacttggaaggagccctggacaggtttgcgcagtttttcctgtgccccttgtttgatgcaagttgtaaagacagagaggtgaacgctgtcgattcagaacatgagaagaatgtgatgaacgatgcctggagactcttccagctggaaaaggctacggggaaccccaaacaccccttcagcaaatttgggacaggaaacaaatatactctagagactcggcccaaccaagaaggcatcgacgtaagggaagagctcttgaaatttcactctacgtattattcgtccaatctgatggcgatttgtgttttaggtcgagaatccttagacgacctcactaacctggtggtgaagctgttctccgaggtggagaacaagaacgttcctctgcctgagttccctgagcaccctttccaggaagagcatctcaggcaactttataaaatagtacccattaaggatattagaaatctttatgtgacatttcccataccagaccttcagcagtactacaaatccaatcctggtcattatcttggtcatctaattgggcacgaaggtcctggaagcctgttgtcagaactcaaatcaaagggctgggtgaataccctggttggtggacagaaggaaggagcccgaggttttatgttttttatcattaatgtggacttaactgaagaagggttattgcacgttgaagatataattttgcacatgtttcaatacattcagaagctacgtgcagaaggacctcaagaatgggttttccaagagtgcaaggacttgaatgctgttgctttcagatttaaagataaagagagaccacgaggctatacgtccaagattgcaggaaagttgcactattatcccctaaatggagtgctcacagctgaatatctattggaggaatttagacccgacttgatagacatggttcttgataaactcagaccagaaaatgtgcgggtggcaatagtttccaaatcatttgaagggaaaactgaccgcacagagcagtggtatggaacacagtataagcaagaagctatcccagaggacatcattcagaaatggcaaaatgctgaccttaatggaaaatttaagcttccaacaaagaatgaatttattcccacaaattttgagattttgtcattagaaaaagatgcaacaccataccctgctcttattaaggatacagccatgagtaagctgtggttcaaacaagatgataaatttttcttgccgaaagcttgtctcaactttgaatttttcagcccgtttgcttatgtggaccccttgcactgtaacatggcctatttgtaccttgagctcctcaaagactcactcaacgagtatgcatatgcagcagagctagcaggcctgagctatgacctccaaaataccatctatgggatgtatctctcagtgaaaggttacaatgacaaacagccaattttgctgaagaagatcaccgagaaaatggctacttttgagattgataagaagagatttgaaattatcaaagaggcctatatgcgatctcttaataacttccgggctgagcagcctcaccagcatgccatgtactacctccgcttgctgatgactgaagtggcctggactaaagatgagctaaaagaagcccttgacgatgttaccctcccccgacttaaggccttcatacctcagctgctgtcacggctgcatattgaagcacttctccatggaaacataaccaagcaggctgccttaggagttatgcagatggtagaagacactcttatcgagcatgctcacaccaaacctctccttccaagtcagctagtccggtacagagaagtccagctccctgacagaggatggtttgtttatcagcagaggaatgaagttcacaataactgcggcatcgagatctactaccagacagacatgcagagcacctcggagaacatgttcctggagctgttttgccagattatctcggagccttgcttcaataccctgcgcaccaaggagcagcttggctatattgtcttcagtggaccccgtcgggccaacggtatccaaggcttgcgcttcatcatccagtcagaaaaaccaccccactacctggagagcagagtagaagccttcttgattaccatggaaaaggccatagaggacatgacagaggaggcgttccaaaaacacattcaagcactagcgattcggcgactcgacaaaccaaagaaactctctgcggagtgtgctaaatactggggggaaatcatctcgcagcagtacaattacgacagagataacatagaagttgcatatttaaagacacttaccaaggacgatatcatcagattctaccaggaaatgttggctgtcgacgcaccgaggagacataaagtgtctgtccatgttcttgccagggaaatggattcttgtcctgttgttggagagttcccctctcagaatgatataaacttgtccgaagcgccacccttgccacaacctgaagtgattcacaacatgactgagttcaagcgtggcctgccactgtttcccctcgtgaaaccacatattaacttcatggctgcaaaactctgataataa BBa_B0034_sequence 1 aaagaggagaaa BBa_I761002_sequence 1 atggacaaattcgacgctaatcgccgcaaattgctggcgcttggtggcgttgcactcggtgccgccatcctgccgacccctgcgtttgcaggcattgtggaacaatgctgtaccagcatctgctccctctaccagctggagaactactgca BBa_B0011_sequence 1 agagaatataaaaagccagattattaatccggcttttttattattt BBa_I761003_sequence 1 atggacaaattcgacgctaatcgccgcaaattgctggcgcttggtggcgttgcactcggtgccgccatcctgccgacccctgcgtttgcatttgtgaaccaacacctgtgcggctcacacctggtggaagctctctacctagtgtgcggggaacgaggcttcttctacacacccaagacctaataa BBa_I761009_sequence 1 tcccttgcatttacattttgaaacatctatagcgataaatgaaacatcttaaaagttttagtatcatattcgtgttggattattctgcatttttggggagaatggacttactagagaaagaggagaaatactagatggacaaattcgacgctaatcgccgcaaattgctggcgcttggtggcgttgcactcggtgccgccatcctgccgacccctgcgtttgcaggcattgtggaacaatgctgtaccagcatctgctccctctaccagctggagaactactgcatactagagaaagaggagaaatactagatggacaaattcgacgctaatcgccgcaaattgctggcgcttggtggcgttgcactcggtgccgccatcctgccgacccctgcgtttgcatttgtgaaccaacacctgtgcggctcacacctggtggaagctctctacctagtgtgcggggaacgaggcttcttctacacacccaagacctaataatactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattttactagagccctttgtgcgtccaaacggacgcacggcgctctaaagcgggtcgcgatctttcagattcgctcctcgcgctttcagtctttgttttggcgcatgtcgttatcgcaaaaccgctgcacacttttgcgcgacatgctctgatccccctcatctgggggggcctatctgagggaatttccgatccggctcgcctgaaccattctgctttccacgaacttgaaaacgctactagagtacattttgaaacatctatagcgataaatgaaacatcttaaaagttttagtatcatattcgttactagagaaagaggagaaatactagatggacaaattcgacgctaatcgccgcaaattgctggcgcttggtggcgttgcactcggtgccgccatcctgccgacccctgcgtttgcaatgcggaacgggctcgtgtggctgctgcaccccgcgctgcccggcaccttgcgctccatcctcggcgcccgcccgccgcccgcgaagcgactgtgtggattcccaaaacagacttacagcacaatgagtaatccggccatccagagaatagaagaccaaattgtcaagtctcctgaagacaaacgggaataccgtggactagagctggccaatggcatcaaagtgcttctcatcagcgatcccaccacagacaagtcctcagcggccctcgatgtgcacataggttcactgtcagaccctccaaatattcctggcttaagtcatttttgtgaacatatgctgtttttgggaaccaagaaatatcctaaagaaaatgaatatagccagtttctcagtgaacatgctggaagttcaaatgcattcaccagtggagaacacaccaattattatttcgatgtttcccatgaacacttggaaggagccctggacaggtttgcgcagtttttcctgtgccccttgtttgatgcaagttgtaaagacagagaggtgaacgctgtcgattcagaacatgagaagaatgtgatgaacgatgcctggagactcttccagctggaaaaggctacggggaaccccaaacaccccttcagcaaatttgggacaggaaacaaatatactctagagactcggcccaaccaagaaggcatcgacgtaagggaagagctcttgaaatttcactctacgtattattcgtccaatctgatggcgatttgtgttttaggtcgagaatccttagacgacctcactaacctggtggtgaagctgttctccgaggtggagaacaagaacgttcctctgcctgagttccctgagcaccctttccaggaagagcatctcaggcaactttataaaatagtacccattaaggatattagaaatctttatgtgacatttcccataccagaccttcagcagtactacaaatccaatcctggtcattatcttggtcatctaattgggcacgaaggtcctggaagcctgttgtcagaactcaaatcaaagggctgggtgaataccctggttggtggacagaaggaaggagcccgaggttttatgttttttatcattaatgtggacttaactgaagaagggttattgcacgttgaagatataattttgcacatgtttcaatacattcagaagctacgtgcagaaggacctcaagaatgggttttccaagagtgcaaggacttgaatgctgttgctttcagatttaaagataaagagagaccacgaggctatacgtccaagattgcaggaaagttgcactattatcccctaaatggagtgctcacagctgaatatctattggaggaatttagacccgacttgatagacatggttcttgataaactcagaccagaaaatgtgcgggtggcaatagtttccaaatcatttgaagggaaaactgaccgcacagagcagtggtatggaacacagtataagcaagaagctatcccagaggacatcattcagaaatggcaaaatgctgaccttaatggaaaatttaagcttccaacaaagaatgaatttattcccacaaattttgagattttgtcattagaaaaagatgcaacaccataccctgctcttattaaggatacagccatgagtaagctgtggttcaaacaagatgataaatttttcttgccgaaagcttgtctcaactttgaatttttcagcccgtttgcttatgtggaccccttgcactgtaacatggcctatttgtaccttgagctcctcaaagactcactcaacgagtatgcatatgcagcagagctagcaggcctgagctatgacctccaaaataccatctatgggatgtatctctcagtgaaaggttacaatgacaaacagccaattttgctgaagaagatcaccgagaaaatggctacttttgagattgataagaagagatttgaaattatcaaagaggcctatatgcgatctcttaataacttccgggctgagcagcctcaccagcatgccatgtactacctccgcttgctgatgactgaagtggcctggactaaagatgagctaaaagaagcccttgacgatgttaccctcccccgacttaaggccttcatacctcagctgctgtcacggctgcatattgaagcacttctccatggaaacataaccaagcaggctgccttaggagttatgcagatggtagaagacactcttatcgagcatgctcacaccaaacctctccttccaagtcagctagtccggtacagagaagtccagctccctgacagaggatggtttgtttatcagcagaggaatgaagttcacaataactgcggcatcgagatctactaccagacagacatgcagagcacctcggagaacatgttcctggagctgttttgccagattatctcggagccttgcttcaataccctgcgcaccaaggagcagcttggctatattgtcttcagtggaccccgtcgggccaacggtatccaaggcttgcgcttcatcatccagtcagaaaaaccaccccactacctggagagcagagtagaagccttcttgattaccatggaaaaggccatagaggacatgacagaggaggcgttccaaaaacacattcaagcactagcgattcggcgactcgacaaaccaaagaaactctctgcggagtgtgctaaatactggggggaaatcatctcgcagcagtacaattacgacagagataacatagaagttgcatatttaaagacacttaccaaggacgatatcatcagattctaccaggaaatgttggctgtcgacgcaccgaggagacataaagtgtctgtccatgttcttgccagggaaatggattcttgtcctgttgttggagagttcccctctcagaatgatataaacttgtccgaagcgccacccttgccacaacctgaagtgattcacaacatgactgagttcaagcgtggcctgccactgtttcccctcgtgaaaccacatattaacttcatggctgcaaaactctgataataatactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattt BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z