##gff-version 3
##sequence-region BBa_I761009 1 4188
BBa_I761009	.	ribosome_entry_site	117	128	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_I761009	.	conserved_region	121	124	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I761009	.	CDS	312	497	.	+	0	ID=BBa_I761003;Name=BBa_I761003
BBa_I761009	.	CDS	400	491	.	+	0	ID=annotation1949761;Name=Human insulin B chain;Parent=BBa_I761003
BBa_I761009	.	conserved_region	315	399	.	+	0	ID=annotation1949760;Name=TAT peptide export signal;Parent=BBa_I761003
BBa_I761009	.	CDS	135	285	.	+	0	ID=BBa_I761002;Name=BBa_I761002
BBa_I761009	.	CDS	223	285	.	+	0	ID=annotation1949759;Name=Human insulin A fragment;Parent=BBa_I761002
BBa_I761009	.	conserved_region	138	222	.	+	0	ID=annotation1949758;Name=TAT peptide export signal;Parent=BBa_I761002
BBa_I761009	.	CDS	930	4085	.	+	0	ID=BBa_I761004;Name=BBa_I761004
BBa_I761009	.	CDS	1018	4079	.	+	0	ID=annotation1949763;Name=Mouse insulin degradation enzyme;Parent=BBa_I761004
BBa_I761009	.	conserved_region	933	1017	.	+	0	ID=annotation1949762;Name=TAT peptide export signal;Parent=BBa_I761004
BBa_I761009	.	ribosome_entry_site	912	923	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_I761009	.	conserved_region	916	919	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I761009	.	terminator	4143	4188	.	+	0	ID=BBa_B0011;Name=BBa_B0011
BBa_I761009	.	stem_loop	4155	4177	.	+	0	ID=annotation1683;Name=stem_loop;Parent=BBa_B0011
BBa_I761009	.	engineered_region	4143	4188	.	+	0	ID=annotation7019;Name=BBa_B0011;Parent=BBa_B0011
BBa_I761009	.	terminator	506	546	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_I761009	.	engineered_region	506	546	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I761009	.	stop_codon	539	539	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I761009	.	stem_loop	513	532	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I761009	.	polyA_site	533	546	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I761009	.	promoter	609	833	.	+	0	ID=BBa_R0078;Name=BBa_R0078
BBa_I761009	.	sequence_feature	609	828	.	+	0	ID=annotation318234;Name=unidentified/uncharacterized CinR dependent;Parent=BBa_R0078
BBa_I761009	.	stem_loop	643	682	.	+	0	ID=annotation318244;Name=stem_loop;Parent=BBa_R0078
BBa_I761009	.	stem_loop	699	744	.	+	0	ID=annotation318250;Name=stem_loop;Parent=BBa_R0078
BBa_I761009	.	promoter	1	108	.	+	0	ID=BBa_R0082;Name=BBa_R0082
BBa_I761009	.	promoter	98	103	.	+	0	ID=annotation301167;Name=-10;Parent=BBa_R0082
BBa_I761009	.	promoter	75	80	.	+	0	ID=annotation301166;Name=-35;Parent=BBa_R0082
BBa_I761009	.	non_covalent_binding_site	13	30	.	+	0	ID=annotation301154;Name=C1 OmpR;Parent=BBa_R0082
BBa_I761009	.	non_covalent_binding_site	34	51	.	+	0	ID=annotation301155;Name=C2 OmpR;Parent=BBa_R0082
BBa_I761009	.	non_covalent_binding_site	54	71	.	+	0	ID=annotation301156;Name=C3 OmpR;Parent=BBa_R0082
BBa_I761009	.	promoter	842	903	.	+	0	ID=BBa_I761001;Name=BBa_I761001
BBa_I761009	.	non_covalent_binding_site	884	901	.	+	0	ID=annotation1944250;Name=C3 OmpR;Parent=BBa_I761001
BBa_I761009	.	non_covalent_binding_site	863	881	.	+	0	ID=annotation1944249;Name=C2 OmpR;Parent=BBa_I761001
BBa_I761009	.	non_covalent_binding_site	843	860	.	+	0	ID=annotation1944248;Name=C1 OmpR;Parent=BBa_I761001
BBa_I761009	.	terminator	555	600	.	+	0	ID=BBa_B0011;Name=BBa_B0011
BBa_I761009	.	stem_loop	567	589	.	+	0	ID=annotation1683;Name=stem_loop;Parent=BBa_B0011
BBa_I761009	.	engineered_region	555	600	.	+	0	ID=annotation7019;Name=BBa_B0011;Parent=BBa_B0011
BBa_I761009	.	ribosome_entry_site	294	305	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_I761009	.	conserved_region	298	301	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I761009	.	terminator	4094	4134	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_I761009	.	engineered_region	4094	4134	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I761009	.	stop_codon	4127	4127	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I761009	.	stem_loop	4101	4120	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I761009	.	polyA_site	4121	4134	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
>BBa_I761009
tcccttgcatttacattttgaaacatctatagcgataaatgaaacatcttaaaagttttagtatcatattcgtgttgga
ttattctgcatttttggggagaatggacttactagagaaagaggagaaatactagatggacaaattcgacgctaatcgc
cgcaaattgctggcgcttggtggcgttgcactcggtgccgccatcctgccgacccctgcgtttgcaggcattgtggaac
aatgctgtaccagcatctgctccctctaccagctggagaactactgcatactagagaaagaggagaaatactagatgga
caaattcgacgctaatcgccgcaaattgctggcgcttggtggcgttgcactcggtgccgccatcctgccgacccctgcg
tttgcatttgtgaaccaacacctgtgcggctcacacctggtggaagctctctacctagtgtgcggggaacgaggcttct
tctacacacccaagacctaataatactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactaga
gagagaatataaaaagccagattattaatccggcttttttattattttactagagccctttgtgcgtccaaacggacgc
acggcgctctaaagcgggtcgcgatctttcagattcgctcctcgcgctttcagtctttgttttggcgcatgtcgttatc
gcaaaaccgctgcacacttttgcgcgacatgctctgatccccctcatctgggggggcctatctgagggaatttccgatc
cggctcgcctgaaccattctgctttccacgaacttgaaaacgctactagagtacattttgaaacatctatagcgataaa
tgaaacatcttaaaagttttagtatcatattcgttactagagaaagaggagaaatactagatggacaaattcgacgcta
atcgccgcaaattgctggcgcttggtggcgttgcactcggtgccgccatcctgccgacccctgcgtttgcaatgcggaa
cgggctcgtgtggctgctgcaccccgcgctgcccggcaccttgcgctccatcctcggcgcccgcccgccgcccgcgaag
cgactgtgtggattcccaaaacagacttacagcacaatgagtaatccggccatccagagaatagaagaccaaattgtca
agtctcctgaagacaaacgggaataccgtggactagagctggccaatggcatcaaagtgcttctcatcagcgatcccac
cacagacaagtcctcagcggccctcgatgtgcacataggttcactgtcagaccctccaaatattcctggcttaagtcat
ttttgtgaacatatgctgtttttgggaaccaagaaatatcctaaagaaaatgaatatagccagtttctcagtgaacatg
ctggaagttcaaatgcattcaccagtggagaacacaccaattattatttcgatgtttcccatgaacacttggaaggagc
cctggacaggtttgcgcagtttttcctgtgccccttgtttgatgcaagttgtaaagacagagaggtgaacgctgtcgat
tcagaacatgagaagaatgtgatgaacgatgcctggagactcttccagctggaaaaggctacggggaaccccaaacacc
ccttcagcaaatttgggacaggaaacaaatatactctagagactcggcccaaccaagaaggcatcgacgtaagggaaga
gctcttgaaatttcactctacgtattattcgtccaatctgatggcgatttgtgttttaggtcgagaatccttagacgac
ctcactaacctggtggtgaagctgttctccgaggtggagaacaagaacgttcctctgcctgagttccctgagcaccctt
tccaggaagagcatctcaggcaactttataaaatagtacccattaaggatattagaaatctttatgtgacatttcccat
accagaccttcagcagtactacaaatccaatcctggtcattatcttggtcatctaattgggcacgaaggtcctggaagc
ctgttgtcagaactcaaatcaaagggctgggtgaataccctggttggtggacagaaggaaggagcccgaggttttatgt
tttttatcattaatgtggacttaactgaagaagggttattgcacgttgaagatataattttgcacatgtttcaatacat
tcagaagctacgtgcagaaggacctcaagaatgggttttccaagagtgcaaggacttgaatgctgttgctttcagattt
aaagataaagagagaccacgaggctatacgtccaagattgcaggaaagttgcactattatcccctaaatggagtgctca
cagctgaatatctattggaggaatttagacccgacttgatagacatggttcttgataaactcagaccagaaaatgtgcg
ggtggcaatagtttccaaatcatttgaagggaaaactgaccgcacagagcagtggtatggaacacagtataagcaagaa
gctatcccagaggacatcattcagaaatggcaaaatgctgaccttaatggaaaatttaagcttccaacaaagaatgaat
ttattcccacaaattttgagattttgtcattagaaaaagatgcaacaccataccctgctcttattaaggatacagccat
gagtaagctgtggttcaaacaagatgataaatttttcttgccgaaagcttgtctcaactttgaatttttcagcccgttt
gcttatgtggaccccttgcactgtaacatggcctatttgtaccttgagctcctcaaagactcactcaacgagtatgcat
atgcagcagagctagcaggcctgagctatgacctccaaaataccatctatgggatgtatctctcagtgaaaggttacaa
tgacaaacagccaattttgctgaagaagatcaccgagaaaatggctacttttgagattgataagaagagatttgaaatt
atcaaagaggcctatatgcgatctcttaataacttccgggctgagcagcctcaccagcatgccatgtactacctccgct
tgctgatgactgaagtggcctggactaaagatgagctaaaagaagcccttgacgatgttaccctcccccgacttaaggc
cttcatacctcagctgctgtcacggctgcatattgaagcacttctccatggaaacataaccaagcaggctgccttagga
gttatgcagatggtagaagacactcttatcgagcatgctcacaccaaacctctccttccaagtcagctagtccggtaca
gagaagtccagctccctgacagaggatggtttgtttatcagcagaggaatgaagttcacaataactgcggcatcgagat
ctactaccagacagacatgcagagcacctcggagaacatgttcctggagctgttttgccagattatctcggagccttgc
ttcaataccctgcgcaccaaggagcagcttggctatattgtcttcagtggaccccgtcgggccaacggtatccaaggct
tgcgcttcatcatccagtcagaaaaaccaccccactacctggagagcagagtagaagccttcttgattaccatggaaaa
ggccatagaggacatgacagaggaggcgttccaaaaacacattcaagcactagcgattcggcgactcgacaaaccaaag
aaactctctgcggagtgtgctaaatactggggggaaatcatctcgcagcagtacaattacgacagagataacatagaag
ttgcatatttaaagacacttaccaaggacgatatcatcagattctaccaggaaatgttggctgtcgacgcaccgaggag
acataaagtgtctgtccatgttcttgccagggaaatggattcttgtcctgttgttggagagttcccctctcagaatgat
ataaacttgtccgaagcgccacccttgccacaacctgaagtgattcacaacatgactgagttcaagcgtggcctgccac
tgtttcccctcgtgaaaccacatattaacttcatggctgcaaaactctgataataatactagagtcacactggctcacc
ttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattt
t