BBa_I761004 1 BBa_I761004 TAT signal+IDE 2007-10-18T11:00:00Z 2015-08-31T04:08:08Z NCBI accession number: BC041675 K12 E. coli genomic DNA Twin arginin signal peptide is an efficient protein export signal sequence of E. coli. The purpose of this construct is to allow E. coli export mouse insulin degradation enzyme(IDE) automatically, allowing controlled insulin degradation of extracellular insulin. false false _140_ 0 2031 9 Not in stock false To combine TAT signal and mouse IDE, we used two separate PCR reaction to amplify each DNA fragment. We design the reverse primer of TAT signal and the forward primer of mouse IDE to be complement to each other, so when we mixed the product of each PCR reaction together, than used the forward primer of TAT signal and the reverse primer of mouse IDE to conduct PCR reaction, we can isolate the combine DNA product of TAT signal and mouse IDE. (Method devised by Dr. Chang) false 2007 NYMU_Taiwan iGEM Team annotation1949763 1 Mouse insulin degradation enzyme range1949763 1 89 3150 annotation1949762 1 TAT peptide export signal range1949762 1 4 88 BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_I761015 1 BBa_I761015 B0034:I761004 2007-10-25T11:00:00Z 2015-08-31T04:08:08Z BioBrick Twin arginin signal peptide is an efficient protein export signal sequence of ''E. coli''. The purpose of this construct is to allow ''E. coli'' export mouse insulin degradation enzyme(IDE) automatically, allowing controlled insulin degradation of extracellular insulin. false false _140_ 0 2032 9 Not in stock false Comparing to BBa_I761004, RBS B0034 is added in the end of this construct false 2007 NYMU_Taiwan iGEM Team component1957801 1 BBa_I761004 component1957798 1 BBa_B0034 annotation1957801 1 BBa_I761004 range1957801 1 19 3174 annotation1957798 1 BBa_B0034 range1957798 1 1 12 BBa_I761004_sequence 1 atggacaaattcgacgctaatcgccgcaaattgctggcgcttggtggcgttgcactcggtgccgccatcctgccgacccctgcgtttgcaatgcggaacgggctcgtgtggctgctgcaccccgcgctgcccggcaccttgcgctccatcctcggcgcccgcccgccgcccgcgaagcgactgtgtggattcccaaaacagacttacagcacaatgagtaatccggccatccagagaatagaagaccaaattgtcaagtctcctgaagacaaacgggaataccgtggactagagctggccaatggcatcaaagtgcttctcatcagcgatcccaccacagacaagtcctcagcggccctcgatgtgcacataggttcactgtcagaccctccaaatattcctggcttaagtcatttttgtgaacatatgctgtttttgggaaccaagaaatatcctaaagaaaatgaatatagccagtttctcagtgaacatgctggaagttcaaatgcattcaccagtggagaacacaccaattattatttcgatgtttcccatgaacacttggaaggagccctggacaggtttgcgcagtttttcctgtgccccttgtttgatgcaagttgtaaagacagagaggtgaacgctgtcgattcagaacatgagaagaatgtgatgaacgatgcctggagactcttccagctggaaaaggctacggggaaccccaaacaccccttcagcaaatttgggacaggaaacaaatatactctagagactcggcccaaccaagaaggcatcgacgtaagggaagagctcttgaaatttcactctacgtattattcgtccaatctgatggcgatttgtgttttaggtcgagaatccttagacgacctcactaacctggtggtgaagctgttctccgaggtggagaacaagaacgttcctctgcctgagttccctgagcaccctttccaggaagagcatctcaggcaactttataaaatagtacccattaaggatattagaaatctttatgtgacatttcccataccagaccttcagcagtactacaaatccaatcctggtcattatcttggtcatctaattgggcacgaaggtcctggaagcctgttgtcagaactcaaatcaaagggctgggtgaataccctggttggtggacagaaggaaggagcccgaggttttatgttttttatcattaatgtggacttaactgaagaagggttattgcacgttgaagatataattttgcacatgtttcaatacattcagaagctacgtgcagaaggacctcaagaatgggttttccaagagtgcaaggacttgaatgctgttgctttcagatttaaagataaagagagaccacgaggctatacgtccaagattgcaggaaagttgcactattatcccctaaatggagtgctcacagctgaatatctattggaggaatttagacccgacttgatagacatggttcttgataaactcagaccagaaaatgtgcgggtggcaatagtttccaaatcatttgaagggaaaactgaccgcacagagcagtggtatggaacacagtataagcaagaagctatcccagaggacatcattcagaaatggcaaaatgctgaccttaatggaaaatttaagcttccaacaaagaatgaatttattcccacaaattttgagattttgtcattagaaaaagatgcaacaccataccctgctcttattaaggatacagccatgagtaagctgtggttcaaacaagatgataaatttttcttgccgaaagcttgtctcaactttgaatttttcagcccgtttgcttatgtggaccccttgcactgtaacatggcctatttgtaccttgagctcctcaaagactcactcaacgagtatgcatatgcagcagagctagcaggcctgagctatgacctccaaaataccatctatgggatgtatctctcagtgaaaggttacaatgacaaacagccaattttgctgaagaagatcaccgagaaaatggctacttttgagattgataagaagagatttgaaattatcaaagaggcctatatgcgatctcttaataacttccgggctgagcagcctcaccagcatgccatgtactacctccgcttgctgatgactgaagtggcctggactaaagatgagctaaaagaagcccttgacgatgttaccctcccccgacttaaggccttcatacctcagctgctgtcacggctgcatattgaagcacttctccatggaaacataaccaagcaggctgccttaggagttatgcagatggtagaagacactcttatcgagcatgctcacaccaaacctctccttccaagtcagctagtccggtacagagaagtccagctccctgacagaggatggtttgtttatcagcagaggaatgaagttcacaataactgcggcatcgagatctactaccagacagacatgcagagcacctcggagaacatgttcctggagctgttttgccagattatctcggagccttgcttcaataccctgcgcaccaaggagcagcttggctatattgtcttcagtggaccccgtcgggccaacggtatccaaggcttgcgcttcatcatccagtcagaaaaaccaccccactacctggagagcagagtagaagccttcttgattaccatggaaaaggccatagaggacatgacagaggaggcgttccaaaaacacattcaagcactagcgattcggcgactcgacaaaccaaagaaactctctgcggagtgtgctaaatactggggggaaatcatctcgcagcagtacaattacgacagagataacatagaagttgcatatttaaagacacttaccaaggacgatatcatcagattctaccaggaaatgttggctgtcgacgcaccgaggagacataaagtgtctgtccatgttcttgccagggaaatggattcttgtcctgttgttggagagttcccctctcagaatgatataaacttgtccgaagcgccacccttgccacaacctgaagtgattcacaacatgactgagttcaagcgtggcctgccactgtttcccctcgtgaaaccacatattaacttcatggctgcaaaactctgataataa BBa_B0034_sequence 1 aaagaggagaaa BBa_I761015_sequence 1 aaagaggagaaatactagatggacaaattcgacgctaatcgccgcaaattgctggcgcttggtggcgttgcactcggtgccgccatcctgccgacccctgcgtttgcaatgcggaacgggctcgtgtggctgctgcaccccgcgctgcccggcaccttgcgctccatcctcggcgcccgcccgccgcccgcgaagcgactgtgtggattcccaaaacagacttacagcacaatgagtaatccggccatccagagaatagaagaccaaattgtcaagtctcctgaagacaaacgggaataccgtggactagagctggccaatggcatcaaagtgcttctcatcagcgatcccaccacagacaagtcctcagcggccctcgatgtgcacataggttcactgtcagaccctccaaatattcctggcttaagtcatttttgtgaacatatgctgtttttgggaaccaagaaatatcctaaagaaaatgaatatagccagtttctcagtgaacatgctggaagttcaaatgcattcaccagtggagaacacaccaattattatttcgatgtttcccatgaacacttggaaggagccctggacaggtttgcgcagtttttcctgtgccccttgtttgatgcaagttgtaaagacagagaggtgaacgctgtcgattcagaacatgagaagaatgtgatgaacgatgcctggagactcttccagctggaaaaggctacggggaaccccaaacaccccttcagcaaatttgggacaggaaacaaatatactctagagactcggcccaaccaagaaggcatcgacgtaagggaagagctcttgaaatttcactctacgtattattcgtccaatctgatggcgatttgtgttttaggtcgagaatccttagacgacctcactaacctggtggtgaagctgttctccgaggtggagaacaagaacgttcctctgcctgagttccctgagcaccctttccaggaagagcatctcaggcaactttataaaatagtacccattaaggatattagaaatctttatgtgacatttcccataccagaccttcagcagtactacaaatccaatcctggtcattatcttggtcatctaattgggcacgaaggtcctggaagcctgttgtcagaactcaaatcaaagggctgggtgaataccctggttggtggacagaaggaaggagcccgaggttttatgttttttatcattaatgtggacttaactgaagaagggttattgcacgttgaagatataattttgcacatgtttcaatacattcagaagctacgtgcagaaggacctcaagaatgggttttccaagagtgcaaggacttgaatgctgttgctttcagatttaaagataaagagagaccacgaggctatacgtccaagattgcaggaaagttgcactattatcccctaaatggagtgctcacagctgaatatctattggaggaatttagacccgacttgatagacatggttcttgataaactcagaccagaaaatgtgcgggtggcaatagtttccaaatcatttgaagggaaaactgaccgcacagagcagtggtatggaacacagtataagcaagaagctatcccagaggacatcattcagaaatggcaaaatgctgaccttaatggaaaatttaagcttccaacaaagaatgaatttattcccacaaattttgagattttgtcattagaaaaagatgcaacaccataccctgctcttattaaggatacagccatgagtaagctgtggttcaaacaagatgataaatttttcttgccgaaagcttgtctcaactttgaatttttcagcccgtttgcttatgtggaccccttgcactgtaacatggcctatttgtaccttgagctcctcaaagactcactcaacgagtatgcatatgcagcagagctagcaggcctgagctatgacctccaaaataccatctatgggatgtatctctcagtgaaaggttacaatgacaaacagccaattttgctgaagaagatcaccgagaaaatggctacttttgagattgataagaagagatttgaaattatcaaagaggcctatatgcgatctcttaataacttccgggctgagcagcctcaccagcatgccatgtactacctccgcttgctgatgactgaagtggcctggactaaagatgagctaaaagaagcccttgacgatgttaccctcccccgacttaaggccttcatacctcagctgctgtcacggctgcatattgaagcacttctccatggaaacataaccaagcaggctgccttaggagttatgcagatggtagaagacactcttatcgagcatgctcacaccaaacctctccttccaagtcagctagtccggtacagagaagtccagctccctgacagaggatggtttgtttatcagcagaggaatgaagttcacaataactgcggcatcgagatctactaccagacagacatgcagagcacctcggagaacatgttcctggagctgttttgccagattatctcggagccttgcttcaataccctgcgcaccaaggagcagcttggctatattgtcttcagtggaccccgtcgggccaacggtatccaaggcttgcgcttcatcatccagtcagaaaaaccaccccactacctggagagcagagtagaagccttcttgattaccatggaaaaggccatagaggacatgacagaggaggcgttccaaaaacacattcaagcactagcgattcggcgactcgacaaaccaaagaaactctctgcggagtgtgctaaatactggggggaaatcatctcgcagcagtacaattacgacagagataacatagaagttgcatatttaaagacacttaccaaggacgatatcatcagattctaccaggaaatgttggctgtcgacgcaccgaggagacataaagtgtctgtccatgttcttgccagggaaatggattcttgtcctgttgttggagagttcccctctcagaatgatataaacttgtccgaagcgccacccttgccacaacctgaagtgattcacaacatgactgagttcaagcgtggcctgccactgtttcccctcgtgaaaccacatattaacttcatggctgcaaaactctgataataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z