BBa_B0011
1
BBa_B0011
LuxICDABEG (+/-)
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Derived from luxICDABEG operon terminator of Vibrio fischeri <genbank>AF170104</genbank>.
Released HQ 2013
Bidirectional transcriptional terminator consisting of a 22 bp stem-loop.</p>
false
false
_1_
0
24
7
In stock
false
<P> <P>In the naturally-occuring sequence there is a mismatch in the stem of the stem loop. This can be corrected via an A->G mutation (at position 40 -- sequence coordinate/not MFOLD coordinate). The above sequence does not reflect this mutation (but the MFOLD image does). This terminator's location cannot be found using some inverted repeat detectors like PALINDROME because it is too short and contains a mismatch. This one was found with the help of Tom Knight. It lies between two coding regions that point towards eachother.<P>
true
Reshma Shetty
annotation1683
1
stem_loop
range1683
1
13
35
annotation7019
1
BBa_B0011
range7019
1
1
46
BBa_B0034
1
BBa_B0034
RBS (Elowitz 1999) -- defines RBS efficiency
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
RBS based on Elowitz repressilator.
false
true
_1_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation23325
1
conserved
range23325
1
5
8
BBa_I769102
1
BBa_I769102
Feruloyl-CoA hydratase for vanillin biosynthesis
2007-06-26T11:00:00Z
2015-08-31T04:08:10Z
Genbank accession: AJ536325; Microb Cell Fact. 2007; 6: 13.
Published online 2007 April 16. doi: 10.1186/1475-2859-6-13.
Copyright ?? 2007 Barghini et al; licensee BioMed Central Ltd.
Vanillin production using metabolically engineered Escherichia coli under non-growing conditions
Paolo Barghini,1 Diana Di Gioia,2 Fabio Fava,2 and Maurizio Ruzzi corresponding author1
One of two steps in the bioconversion of ferulic acid to vanillin. The enzyme is from Pseudomonas fluorescens and it does work with E. coli. It requires both ech and fcs for vanillin biosynthesis.
false
false
_162_
0
967
1
Not in stock
false
Changed stop codon to TAATAA
false
Mackenize Cowell
annotation1935577
1
Stop codon
range1935577
1
829
834
annotation1935578
1
Feruloyl-CoA hydratase (ech)
range1935578
1
1
834
annotation1935576
1
Start codon
range1935576
1
1
3
BBa_B0012
1
BBa_B0012
TE from coliphageT7
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
Released HQ 2013
Transcription terminator for the <i>E.coli</i> RNA polymerase.
false
false
_1_
0
24
7
In stock
false
<P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
false
Reshma Shetty
annotation1686
1
T7 TE
range1686
1
8
27
annotation7020
1
BBa_B0012
range7020
1
1
41
annotation1687
1
stop
range1687
1
34
34
annotation1690
1
polya
range1690
1
28
41
BBa_I763002
1
BBa_I763002
ECH protein generator
2007-06-26T11:00:00Z
2015-08-31T04:08:08Z
uses <partinfo>BBa_I769015</partinfo> as well as a promoter, RBS, and terminator
Per vanilla from.....
true
false
_111_
0
1649
9
Discontinued
false
needs to be usede,,,,
false
Silvio Cavalcanti
component1935765
1
BBa_B0034
component1935770
1
BBa_B0012
component1935774
1
BBa_B0011
component1935769
1
BBa_I769102
component1935759
1
BBa_R0011
annotation1935765
1
BBa_B0034
range1935765
1
64
75
annotation1935774
1
BBa_B0011
range1935774
1
973
1018
annotation1935759
1
BBa_R0011
range1935759
1
1
54
annotation1935770
1
BBa_B0012
range1935770
1
924
964
annotation1935769
1
BBa_I769102
range1935769
1
82
915
BBa_R0011
1
lacI+pL
Promoter (lacI regulated, lambda pL hybrid)
2003-01-31T12:00:00Z
2015-05-08T01:14:14Z
represillator of Elowitz and Leibler (2000)
Released HQ 2013
Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) <p> The PLlac 0-1 promoter is a hybrid regulatory region consisting of the promoter P(L) of phage lambda with the cI binding sites replaced with lacO1. The hybrid design allows for strong promotion that can nevertheless be tightly repressed by LacI, the Lac inhibitor (i.e. repressor) (<bb_part>BBa_C0010</bb_part>) ([LUTZ97]). The activity of the promoter can be regulated over a >600-fold range by IPTG in E.Coli DH5-alpha-Z1 (same paper reference).
false
true
_1_
0
24
7
In stock
false
<P> <P>hybrid promoter design to create strong promoter that is, at the same time, highly repressible. note that the upstream operator installed in this hybrid is slightly different than the one in the original source (Lutz and Bujard, 1997). the most upstream operator region is slightly truncated in the represillator version, so that both operators in the hybrid are the same sequence. see references for details. also, the sequence has been truncated after the transcriptional start site.<P>LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and increase transcription. This part is incompatible with environments containing lactose or lactose analogs.
true
Neelaksh Varshney, Grace Kenney, Daniel Shen, Samantha Sutton
annotation2001
1
lac O1
range2001
1
26
42
annotation7064
1
BBa_R0011
range7064
1
1
54
annotation1999
1
lac O1
range1999
1
3
19
annotation2000
1
-35
range2000
1
20
25
annotation2002
1
-10
range2002
1
43
48
BBa_I763002_sequence
1
aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacatactagagaaagaggagaaatactagatgagcaactacgaaggtcgctggacaacggtcaaggtcgaaatcgaagacggcattgcctgggtcattctcaatcgtccggaaaaacgcaacgccatgagcccaaccctgaaccgggaaatgatcgacgtgctggaaaccctggaacaggacccggccgctggtgtgctggtgctgaccggcgccggcgaggcctggaccgccggcatggacctcaaggaatacttccgcgaggtggatgccgggccggagatccttcaggagaaaatccgccgcgaagcctcccagtggcagtggaaactgctgcgcatgtacgccaagccgaccatcgccatggtcaacggctggtgcttcggtggcggcttcagcccgctggtggcgtgcgatctggcgatctgcgccgacgaggccaccttcggcctgtctgaaatcaactggggcatcccgccgggcaacctggtgagcaaggccatggccgacaccgtgggccatcgccagtcgctgtactacatcatgaccggcaagactttcggcgggcagaaagccgccgaaatgggcctggtcaacgacagcgtgcccctggctcgattgcgtgaggtgaccattgagctggcgcgcaacctgctggagaaaaacccggtggtgctgcgtgccgccaagcatggcttcaagcgttgccgcgagctgacctgggagcagaacgaagactacctgtacgccaagctcgatcagtcgcgcctgttggacaccgaaggcggccgcgagcagggcatgaagcagtttctcgacgacaagagcatcaagcccggcctgcaggcgtataaacgctaataatactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattt
BBa_B0034_sequence
1
aaagaggagaaa
BBa_B0011_sequence
1
agagaatataaaaagccagattattaatccggcttttttattattt
BBa_I769102_sequence
1
atgagcaactacgaaggtcgctggacaacggtcaaggtcgaaatcgaagacggcattgcctgggtcattctcaatcgtccggaaaaacgcaacgccatgagcccaaccctgaaccgggaaatgatcgacgtgctggaaaccctggaacaggacccggccgctggtgtgctggtgctgaccggcgccggcgaggcctggaccgccggcatggacctcaaggaatacttccgcgaggtggatgccgggccggagatccttcaggagaaaatccgccgcgaagcctcccagtggcagtggaaactgctgcgcatgtacgccaagccgaccatcgccatggtcaacggctggtgcttcggtggcggcttcagcccgctggtggcgtgcgatctggcgatctgcgccgacgaggccaccttcggcctgtctgaaatcaactggggcatcccgccgggcaacctggtgagcaaggccatggccgacaccgtgggccatcgccagtcgctgtactacatcatgaccggcaagactttcggcgggcagaaagccgccgaaatgggcctggtcaacgacagcgtgcccctggctcgattgcgtgaggtgaccattgagctggcgcgcaacctgctggagaaaaacccggtggtgctgcgtgccgccaagcatggcttcaagcgttgccgcgagctgacctgggagcagaacgaagactacctgtacgccaagctcgatcagtcgcgcctgttggacaccgaaggcggccgcgagcagggcatgaagcagtttctcgacgacaagagcatcaagcccggcctgcaggcgtataaacgctaataa
BBa_B0012_sequence
1
tcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_R0011_sequence
1
aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcaca
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z