LOCUS       BBa_I763004             1122 bp    DNA     linear       22-Dec-2024
DEFINITION  GFP coding device switched on by IPTG
ACCESSION   BBa_I763004
VERSION     BBa_I763004.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    213..216
                     /label=conserved
     RBS             209..220
                     /label=BBa_B0034
     misc_feature    227..946
                     /label=BBa_E0040
     misc_feature    941..979
                     /label=LVA
     misc_feature    980..985
                     /label=Double Stop
     misc_feature    227..229
                     /label=Start
     misc_feature    227..985
                     /label=BBa_J04031
     stem_loop       1005..1048
                     /label=stem_loop
     misc_feature    994..1073
                     /label=BBa_B0010
     terminator      994..1073
                     /label=BBa_B0010
     stem_loop       1089..1108
                     /label=T7 TE
     misc_feature    1115..1115
                     /label=stop
     polyA_site      1109..1122
                     /label=polya
     misc_feature    1082..1122
                     /label=BBa_B0012
     terminator      1082..1122
                     /label=BBa_B0012
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatgc gtaaaggaga
      241 agaacttttc actggagttg tcccaattct tgttgaatta gatggtgatg ttaatgggca
      301 caaattttct gtcagtggag agggtgaagg tgatgcaaca tacggaaaac ttacccttaa
      361 atttatttgc actactggaa aactacctgt tccatggcca acacttgtca ctactttcgg
      421 ttatggtgtt caatgctttg cgagataccc agatcatatg aaacagcatg actttttcaa
      481 gagtgccatg cccgaaggtt atgtacagga aagaactata tttttcaaag atgacgggaa
      541 ctacaagaca cgtgctgaag tcaagtttga aggtgatacc cttgttaata gaatcgagtt
      601 aaaaggtatt gattttaaag aagatggaaa cattcttgga cacaaattgg aatacaacta
      661 taactcacac aatgtataca tcatggcaga caaacaaaag aatggaatca aagttaactt
      721 caaaattaga cacaacattg aagatggaag cgttcaacta gcagaccatt atcaacaaaa
      781 tactccaatt ggcgatggcc ctgtcctttt accagacaac cattacctgt ccacacaatc
      841 tgccctttcg aaagatccca acgaaaagag agaccacatg gtccttcttg agtttgtaac
      901 agctgctggg attacacatg gcatggatga actatacaaa aggcctgctg caaacgacga
      961 aaactacgct ttagtagctt aataatacta gagccaggca tcaaataaaa cgaaaggctc
     1021 agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctctactaga
     1081 gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta ta
//