LOCUS       BBa_I763014             2315 bp    DNA     linear       22-Dec-2024
DEFINITION  LacY and cI regulated by PLac Promoter
ACCESSION   BBa_I763014
VERSION     BBa_I763014.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    213..216
                     /label=conserved
     RBS             209..220
                     /label=BBa_B0034
     CDS             227..1514
                     /label=BBa_J22101
     misc_feature    1..1514
                     /label=BBa_I763012
     misc_feature    1527..1530
                     /label=conserved
     RBS             1523..1534
                     /label=BBa_B0034
     CDS             1544..2251
                     /label=cI lambda
     misc_feature    2252..2284
                     /label=LVA
     misc_feature    2291..2315
                     /label=Help:Barcodes
     CDS             1541..2290
                     /label=BBa_C0051
     misc_feature    1523..2315
                     /label=BBa_J06550
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatgt ctgcccgtat
      241 ttcgcgtaag gaaatccatt atgtactatt taaaaaacac aaacttttgg atgttcggtt
      301 tattcttttt cttttacttt tttatcatgg gagcctactt cccgtttttc ccgatttggc
      361 tacatgacat caaccatatc agcaaaagtg atacgggtat tatttttgcc gctatttctc
      421 tgttctcgct attattccaa ccgctgtttg gtctgctttc tgacaaactc gggctgcgca
      481 aatacctgct gtggattatt accggcatgt tagtgatgtt tgcgccgttc tttattttta
      541 tcttcgggcc actgttacaa tacaacattt tagtaggatc gattgttggt ggtatttatc
      601 taggcttttg ttttaacgcc ggtgcgccag cagtagaggc atttattgag aaagtcagcc
      661 gtcgcagtaa tttcgaattt ggtcgcgcgc ggatgtttgg ctgtgttggc tgggcgctgt
      721 gtgcctcgat tgtcggcatc atgttcacca tcaataatca gtttgttttc tggctgggct
      781 ctggctgtgc actcatcctc gccgttttac tctttttcgc caaaacggat gcgccctctt
      841 ctgccacggt tgccaatgcg gtaggtgcca accattcggc atttagcctt aagctggcac
      901 tggaactgtt cagacagcca aaactgtggt ttttgtcact gtatgttatt ggcgtttcct
      961 gcacctacga tgtttttgac caacagtttg ctaatttctt tacttcgttc tttgctaccg
     1021 gtgaacaggg tacgcgggta tttggctacg taacgacaat gggcgaatta cttaacgcct
     1081 cgattatgtt ctttgcgcca ctgatcatta atcgcatcgg tgggaaaaac gccctgctgc
     1141 tggctggcac tattatgtct gtacgtatta ttggctcatc gttcgccacc tcagcgctgg
     1201 aagtggttat tctgaaaacg ctgcatatgt ttgaagtacc gttcctgctg gtgggctgct
     1261 ttaaatatat taccagccag tttgaagtgc gtttttcagc gacgatttat ctggtctgtt
     1321 tctgcttctt taagcaactg gcgatgattt ttatgtctgt actggcgggc aatatgtatg
     1381 aaagcatcgg tttccagggc gcttatctgg tgctgggtct ggtggcgctg ggcttcacct
     1441 taatttccgt gttcacgctt agcggccccg gcccgctttc cctgctgcgt cgtcaggtga
     1501 atgaagtcgc ttaatactag agaaagagga gaaatactag atgagcacaa aaaagaaacc
     1561 attaacacaa gagcagcttg aggacgcacg tcgccttaaa gcaatttatg aaaaaaagaa
     1621 aaatgaactt ggcttatccc aggaatctgt cgcagacaag atggggatgg ggcagtcagg
     1681 cgttggtgct ttatttaatg gcatcaatgc attaaatgct tataacgccg cattgcttgc
     1741 aaaaattctc aaagttagcg ttgaagaatt tagcccttca atcgccagag aaatctacga
     1801 gatgtatgaa gcggttagta tgcagccgtc acttagaagt gagtatgagt accctgtttt
     1861 ttctcatgtt caggcaggga tgttctcacc tgagcttaga acctttacca aaggtgatgc
     1921 ggagagatgg gtaagcacaa ccaaaaaagc cagtgattct gcattctggc ttgaggttga
     1981 aggtaattcc atgaccgcac caacaggctc caagccaagc tttcctgacg gaatgttaat
     2041 tctcgttgac cctgagcagg ctgttgagcc aggtgatttc tgcatagcca gacttggggg
     2101 tgatgagttt accttcaaga aactgatcag ggatagcggt caggtgtttt tacaaccact
     2161 aaacccacag tacccaatga tcccatgcaa tgagagttgt tccgttgtgg ggaaagttat
     2221 cgctagtcag tggcctgaag agacgtttgg cgctgcaaac gacgaaaact acgctttagt
     2281 agcttaataa cgctgatagt gctagtgtag atcgc
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