LOCUS       BBa_I763017             3494 bp    DNA     linear       22-Nov-2024
DEFINITION  CI, LacY and LacI coding regions regulated by PLac Promoter
ACCESSION   BBa_I763017
VERSION     BBa_I763017.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    213..216
                     /label=conserved
     RBS             209..220
                     /label=BBa_B0034
     CDS             227..1514
                     /label=BBa_J22101
     misc_feature    1527..1530
                     /label=conserved
     RBS             1523..1534
                     /label=BBa_B0034
     CDS             1544..2251
                     /label=cI lambda
     misc_feature    2252..2284
                     /label=LVA
     misc_feature    2291..2315
                     /label=Help:Barcodes
     CDS             1541..2290
                     /label=BBa_C0051
     misc_feature    2328..2331
                     /label=conserved
     RBS             2324..2335
                     /label=BBa_B0034
     misc_feature    3431..3469
                     /label=LVA
     CDS             2342..3469
                     /label=lacI-LVA
     misc_feature    2342..3469
                     /label=BBa_C0012
     misc_feature    3470..3494
                     /label=Help:Barcodes
     CDS             2342..3469
                     /label=BBa_C0012
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatgt ctgcccgtat
      241 ttcgcgtaag gaaatccatt atgtactatt taaaaaacac aaacttttgg atgttcggtt
      301 tattcttttt cttttacttt tttatcatgg gagcctactt cccgtttttc ccgatttggc
      361 tacatgacat caaccatatc agcaaaagtg atacgggtat tatttttgcc gctatttctc
      421 tgttctcgct attattccaa ccgctgtttg gtctgctttc tgacaaactc gggctgcgca
      481 aatacctgct gtggattatt accggcatgt tagtgatgtt tgcgccgttc tttattttta
      541 tcttcgggcc actgttacaa tacaacattt tagtaggatc gattgttggt ggtatttatc
      601 taggcttttg ttttaacgcc ggtgcgccag cagtagaggc atttattgag aaagtcagcc
      661 gtcgcagtaa tttcgaattt ggtcgcgcgc ggatgtttgg ctgtgttggc tgggcgctgt
      721 gtgcctcgat tgtcggcatc atgttcacca tcaataatca gtttgttttc tggctgggct
      781 ctggctgtgc actcatcctc gccgttttac tctttttcgc caaaacggat gcgccctctt
      841 ctgccacggt tgccaatgcg gtaggtgcca accattcggc atttagcctt aagctggcac
      901 tggaactgtt cagacagcca aaactgtggt ttttgtcact gtatgttatt ggcgtttcct
      961 gcacctacga tgtttttgac caacagtttg ctaatttctt tacttcgttc tttgctaccg
     1021 gtgaacaggg tacgcgggta tttggctacg taacgacaat gggcgaatta cttaacgcct
     1081 cgattatgtt ctttgcgcca ctgatcatta atcgcatcgg tgggaaaaac gccctgctgc
     1141 tggctggcac tattatgtct gtacgtatta ttggctcatc gttcgccacc tcagcgctgg
     1201 aagtggttat tctgaaaacg ctgcatatgt ttgaagtacc gttcctgctg gtgggctgct
     1261 ttaaatatat taccagccag tttgaagtgc gtttttcagc gacgatttat ctggtctgtt
     1321 tctgcttctt taagcaactg gcgatgattt ttatgtctgt actggcgggc aatatgtatg
     1381 aaagcatcgg tttccagggc gcttatctgg tgctgggtct ggtggcgctg ggcttcacct
     1441 taatttccgt gttcacgctt agcggccccg gcccgctttc cctgctgcgt cgtcaggtga
     1501 atgaagtcgc ttaatactag agaaagagga gaaatactag atgagcacaa aaaagaaacc
     1561 attaacacaa gagcagcttg aggacgcacg tcgccttaaa gcaatttatg aaaaaaagaa
     1621 aaatgaactt ggcttatccc aggaatctgt cgcagacaag atggggatgg ggcagtcagg
     1681 cgttggtgct ttatttaatg gcatcaatgc attaaatgct tataacgccg cattgcttgc
     1741 aaaaattctc aaagttagcg ttgaagaatt tagcccttca atcgccagag aaatctacga
     1801 gatgtatgaa gcggttagta tgcagccgtc acttagaagt gagtatgagt accctgtttt
     1861 ttctcatgtt caggcaggga tgttctcacc tgagcttaga acctttacca aaggtgatgc
     1921 ggagagatgg gtaagcacaa ccaaaaaagc cagtgattct gcattctggc ttgaggttga
     1981 aggtaattcc atgaccgcac caacaggctc caagccaagc tttcctgacg gaatgttaat
     2041 tctcgttgac cctgagcagg ctgttgagcc aggtgatttc tgcatagcca gacttggggg
     2101 tgatgagttt accttcaaga aactgatcag ggatagcggt caggtgtttt tacaaccact
     2161 aaacccacag tacccaatga tcccatgcaa tgagagttgt tccgttgtgg ggaaagttat
     2221 cgctagtcag tggcctgaag agacgtttgg cgctgcaaac gacgaaaact acgctttagt
     2281 agcttaataa cgctgatagt gctagtgtag atcgctacta gagaaagagg agaaatacta
     2341 gatggtgaat gtgaaaccag taacgttata cgatgtcgca gagtatgccg gtgtctctta
     2401 tcagaccgtt tcccgcgtgg tgaaccaggc cagccacgtt tctgcgaaaa cgcgggaaaa
     2461 agtggaagcg gcgatggcgg agctgaatta cattcccaac cgcgtggcac aacaactggc
     2521 gggcaaacag tcgttgctga ttggcgttgc cacctccagt ctggccctgc acgcgccgtc
     2581 gcaaattgtc gcggcgatta aatctcgcgc cgatcaactg ggtgccagcg tggtggtgtc
     2641 gatggtagaa cgaagcggcg tcgaagcctg taaagcggcg gtgcacaatc ttctcgcgca
     2701 acgcgtcagt gggctgatca ttaactatcc gctggatgac caggatgcca ttgctgtgga
     2761 agctgcctgc actaatgttc cggcgttatt tcttgatgtc tctgaccaga cacccatcaa
     2821 cagtattatt ttctcccatg aagacggtac gcgactgggc gtggagcatc tggtcgcatt
     2881 gggtcaccag caaatcgcgc tgttagcggg cccattaagt tctgtctcgg cgcgtctgcg
     2941 tctggctggc tggcataaat atctcactcg caatcaaatt cagccgatag cggaacggga
     3001 aggcgactgg agtgccatgt ccggttttca acaaaccatg caaatgctga atgagggcat
     3061 cgttcccact gcgatgctgg ttgccaacga tcagatggcg ctgggcgcaa tgcgcgccat
     3121 taccgagtcc gggctgcgcg ttggtgcgga tatctcggta gtgggatacg acgataccga
     3181 agacagctca tgttatatcc cgccgttaac caccatcaaa caggattttc gcctgctggg
     3241 gcaaaccagc gtggaccgct tgctgcaact ctctcagggc caggcggtga agggcaatca
     3301 gctgttgccc gtctcactgg tgaaaagaaa aaccaccctg gcgcccaata cgcaaaccgc
     3361 ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga
     3421 aagcgggcag gctgcaaacg acgaaaacta cgctttagta gcttaataac tctgatagtg
     3481 ctagtgtaga tctc
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