LOCUS BBa_I763018 4416 bp DNA linear 26-Dec-2024 DEFINITION GFP, LacI, LacY and CI coding device switched on by IPTG ACCESSION BBa_I763018 VERSION BBa_I763018.1 FEATURES Location/Qualifiers CDS 1..88 /label=end of LacI coding region (inactive) misc_feature 1..200 /label=BBa_R0010 misc_feature 89..126 /label=CAP binding site promoter 137..142 /label=-35 promoter 161..166 /label=-10 misc_feature 166..200 /label=LacI binding site misc_feature 173..173 /label=start promoter 1..200 /label=BBa_R0010 misc_feature 213..216 /label=conserved RBS 209..220 /label=BBa_B0034 CDS 227..1514 /label=BBa_J22101 misc_feature 1527..1530 /label=conserved RBS 1523..1534 /label=BBa_B0034 CDS 1544..2251 /label=cI lambda misc_feature 2252..2284 /label=LVA misc_feature 2291..2315 /label=Help:Barcodes CDS 1541..2290 /label=BBa_C0051 misc_feature 2328..2331 /label=conserved RBS 2324..2335 /label=BBa_B0034 misc_feature 3431..3469 /label=LVA CDS 2342..3469 /label=lacI-LVA misc_feature 2342..3469 /label=BBa_C0012 misc_feature 3470..3494 /label=Help:Barcodes CDS 2342..3469 /label=BBa_C0012 misc_feature 3507..3510 /label=conserved RBS 3503..3514 /label=BBa_B0034 misc_feature 3521..4240 /label=BBa_E0040 misc_feature 4235..4273 /label=LVA misc_feature 4274..4279 /label=Double Stop misc_feature 3521..3523 /label=Start misc_feature 3521..4279 /label=BBa_J04031 stem_loop 4299..4342 /label=stem_loop misc_feature 4288..4367 /label=BBa_B0010 terminator 4288..4367 /label=BBa_B0010 stem_loop 4383..4402 /label=T7 TE misc_feature 4409..4409 /label=stop polyA_site 4403..4416 /label=polya misc_feature 4376..4416 /label=BBa_B0012 terminator 4376..4416 /label=BBa_B0012 ORIGIN 1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca 61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc 121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga 181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatgt ctgcccgtat 241 ttcgcgtaag gaaatccatt atgtactatt taaaaaacac aaacttttgg atgttcggtt 301 tattcttttt cttttacttt tttatcatgg gagcctactt cccgtttttc ccgatttggc 361 tacatgacat caaccatatc agcaaaagtg atacgggtat tatttttgcc gctatttctc 421 tgttctcgct attattccaa ccgctgtttg gtctgctttc tgacaaactc gggctgcgca 481 aatacctgct gtggattatt accggcatgt tagtgatgtt tgcgccgttc tttattttta 541 tcttcgggcc actgttacaa tacaacattt tagtaggatc gattgttggt ggtatttatc 601 taggcttttg ttttaacgcc ggtgcgccag cagtagaggc atttattgag aaagtcagcc 661 gtcgcagtaa tttcgaattt ggtcgcgcgc ggatgtttgg ctgtgttggc tgggcgctgt 721 gtgcctcgat tgtcggcatc atgttcacca tcaataatca gtttgttttc tggctgggct 781 ctggctgtgc actcatcctc gccgttttac tctttttcgc caaaacggat gcgccctctt 841 ctgccacggt tgccaatgcg gtaggtgcca accattcggc atttagcctt aagctggcac 901 tggaactgtt cagacagcca aaactgtggt ttttgtcact gtatgttatt ggcgtttcct 961 gcacctacga tgtttttgac caacagtttg ctaatttctt tacttcgttc tttgctaccg 1021 gtgaacaggg tacgcgggta tttggctacg taacgacaat gggcgaatta cttaacgcct 1081 cgattatgtt ctttgcgcca ctgatcatta atcgcatcgg tgggaaaaac gccctgctgc 1141 tggctggcac tattatgtct gtacgtatta ttggctcatc gttcgccacc tcagcgctgg 1201 aagtggttat tctgaaaacg ctgcatatgt ttgaagtacc gttcctgctg gtgggctgct 1261 ttaaatatat taccagccag tttgaagtgc gtttttcagc gacgatttat ctggtctgtt 1321 tctgcttctt taagcaactg gcgatgattt ttatgtctgt actggcgggc aatatgtatg 1381 aaagcatcgg tttccagggc gcttatctgg tgctgggtct ggtggcgctg ggcttcacct 1441 taatttccgt gttcacgctt agcggccccg gcccgctttc cctgctgcgt cgtcaggtga 1501 atgaagtcgc ttaatactag agaaagagga gaaatactag atgagcacaa aaaagaaacc 1561 attaacacaa gagcagcttg aggacgcacg tcgccttaaa gcaatttatg aaaaaaagaa 1621 aaatgaactt ggcttatccc aggaatctgt cgcagacaag atggggatgg ggcagtcagg 1681 cgttggtgct ttatttaatg gcatcaatgc attaaatgct tataacgccg cattgcttgc 1741 aaaaattctc aaagttagcg ttgaagaatt tagcccttca atcgccagag aaatctacga 1801 gatgtatgaa gcggttagta tgcagccgtc acttagaagt gagtatgagt accctgtttt 1861 ttctcatgtt caggcaggga tgttctcacc tgagcttaga acctttacca aaggtgatgc 1921 ggagagatgg gtaagcacaa ccaaaaaagc cagtgattct gcattctggc ttgaggttga 1981 aggtaattcc atgaccgcac caacaggctc caagccaagc tttcctgacg gaatgttaat 2041 tctcgttgac cctgagcagg ctgttgagcc aggtgatttc tgcatagcca gacttggggg 2101 tgatgagttt accttcaaga aactgatcag ggatagcggt caggtgtttt tacaaccact 2161 aaacccacag tacccaatga tcccatgcaa tgagagttgt tccgttgtgg ggaaagttat 2221 cgctagtcag tggcctgaag agacgtttgg cgctgcaaac gacgaaaact acgctttagt 2281 agcttaataa cgctgatagt gctagtgtag atcgctacta gagaaagagg agaaatacta 2341 gatggtgaat gtgaaaccag taacgttata cgatgtcgca gagtatgccg gtgtctctta 2401 tcagaccgtt tcccgcgtgg tgaaccaggc cagccacgtt tctgcgaaaa cgcgggaaaa 2461 agtggaagcg gcgatggcgg agctgaatta cattcccaac cgcgtggcac aacaactggc 2521 gggcaaacag tcgttgctga ttggcgttgc cacctccagt ctggccctgc acgcgccgtc 2581 gcaaattgtc gcggcgatta aatctcgcgc cgatcaactg ggtgccagcg tggtggtgtc 2641 gatggtagaa cgaagcggcg tcgaagcctg taaagcggcg gtgcacaatc ttctcgcgca 2701 acgcgtcagt gggctgatca ttaactatcc gctggatgac caggatgcca ttgctgtgga 2761 agctgcctgc actaatgttc cggcgttatt tcttgatgtc tctgaccaga cacccatcaa 2821 cagtattatt ttctcccatg aagacggtac gcgactgggc gtggagcatc tggtcgcatt 2881 gggtcaccag caaatcgcgc tgttagcggg cccattaagt tctgtctcgg cgcgtctgcg 2941 tctggctggc tggcataaat atctcactcg caatcaaatt cagccgatag cggaacggga 3001 aggcgactgg agtgccatgt ccggttttca acaaaccatg caaatgctga atgagggcat 3061 cgttcccact gcgatgctgg ttgccaacga tcagatggcg ctgggcgcaa tgcgcgccat 3121 taccgagtcc gggctgcgcg ttggtgcgga tatctcggta gtgggatacg acgataccga 3181 agacagctca tgttatatcc cgccgttaac caccatcaaa caggattttc gcctgctggg 3241 gcaaaccagc gtggaccgct tgctgcaact ctctcagggc caggcggtga agggcaatca 3301 gctgttgccc gtctcactgg tgaaaagaaa aaccaccctg gcgcccaata cgcaaaccgc 3361 ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga 3421 aagcgggcag gctgcaaacg acgaaaacta cgctttagta gcttaataac tctgatagtg 3481 ctagtgtaga tctctactag agaaagagga gaaatactag atgcgtaaag gagaagaact 3541 tttcactgga gttgtcccaa ttcttgttga attagatggt gatgttaatg ggcacaaatt 3601 ttctgtcagt ggagagggtg aaggtgatgc aacatacgga aaacttaccc ttaaatttat 3661 ttgcactact ggaaaactac ctgttccatg gccaacactt gtcactactt tcggttatgg 3721 tgttcaatgc tttgcgagat acccagatca tatgaaacag catgactttt tcaagagtgc 3781 catgcccgaa ggttatgtac aggaaagaac tatatttttc aaagatgacg ggaactacaa 3841 gacacgtgct gaagtcaagt ttgaaggtga tacccttgtt aatagaatcg agttaaaagg 3901 tattgatttt aaagaagatg gaaacattct tggacacaaa ttggaataca actataactc 3961 acacaatgta tacatcatgg cagacaaaca aaagaatgga atcaaagtta acttcaaaat 4021 tagacacaac attgaagatg gaagcgttca actagcagac cattatcaac aaaatactcc 4081 aattggcgat ggccctgtcc ttttaccaga caaccattac ctgtccacac aatctgccct 4141 ttcgaaagat cccaacgaaa agagagacca catggtcctt cttgagtttg taacagctgc 4201 tgggattaca catggcatgg atgaactata caaaaggcct gctgcaaacg acgaaaacta 4261 cgctttagta gcttaataat actagagcca ggcatcaaat aaaacgaaag gctcagtcga 4321 aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac tagagtcaca 4381 ctggctcacc ttcgggtggg cctttctgcg tttata //