LOCUS       BBa_I763019             3237 bp    DNA     linear       22-Dec-2024
DEFINITION  CI and LacY coding device switched on by IPTG with GFP as a 
            reporter
ACCESSION   BBa_I763019
VERSION     BBa_I763019.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    213..216
                     /label=conserved
     RBS             209..220
                     /label=BBa_B0034
     misc_feature    1..220
                     /label=BBa_J04500
     CDS             230..937
                     /label=cI lambda
     misc_feature    938..970
                     /label=LVA
     misc_feature    977..1001
                     /label=Help:Barcodes
     CDS             227..1001
                     /label=BBa_C0051
     misc_feature    1..1001
                     /label=BBa_I763005
     misc_feature    1014..1017
                     /label=conserved
     RBS             1010..1021
                     /label=BBa_B0034
     CDS             1028..2315
                     /label=BBa_J22101
     misc_feature    1010..2315
                     /label=BBa_I763015
     misc_feature    1..2315
                     /label=BBa_I763016
     misc_feature    2328..2331
                     /label=conserved
     RBS             2324..2335
                     /label=BBa_B0034
     misc_feature    2342..3061
                     /label=BBa_E0040
     misc_feature    3056..3094
                     /label=LVA
     misc_feature    3095..3100
                     /label=Double Stop
     misc_feature    2342..2344
                     /label=Start
     misc_feature    2342..3100
                     /label=BBa_J04031
     stem_loop       3120..3163
                     /label=stem_loop
     misc_feature    3109..3188
                     /label=BBa_B0010
     terminator      3109..3188
                     /label=BBa_B0010
     stem_loop       3204..3223
                     /label=T7 TE
     misc_feature    3230..3230
                     /label=stop
     polyA_site      3224..3237
                     /label=polya
     misc_feature    3197..3237
                     /label=BBa_B0012
     terminator      3197..3237
                     /label=BBa_B0012
     misc_feature    2324..3237
                     /label=BBa_I763020
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatga gcacaaaaaa
      241 gaaaccatta acacaagagc agcttgagga cgcacgtcgc cttaaagcaa tttatgaaaa
      301 aaagaaaaat gaacttggct tatcccagga atctgtcgca gacaagatgg ggatggggca
      361 gtcaggcgtt ggtgctttat ttaatggcat caatgcatta aatgcttata acgccgcatt
      421 gcttgcaaaa attctcaaag ttagcgttga agaatttagc ccttcaatcg ccagagaaat
      481 ctacgagatg tatgaagcgg ttagtatgca gccgtcactt agaagtgagt atgagtaccc
      541 tgttttttct catgttcagg cagggatgtt ctcacctgag cttagaacct ttaccaaagg
      601 tgatgcggag agatgggtaa gcacaaccaa aaaagccagt gattctgcat tctggcttga
      661 ggttgaaggt aattccatga ccgcaccaac aggctccaag ccaagctttc ctgacggaat
      721 gttaattctc gttgaccctg agcaggctgt tgagccaggt gatttctgca tagccagact
      781 tgggggtgat gagtttacct tcaagaaact gatcagggat agcggtcagg tgtttttaca
      841 accactaaac ccacagtacc caatgatccc atgcaatgag agttgttccg ttgtggggaa
      901 agttatcgct agtcagtggc ctgaagagac gtttggcgct gcaaacgacg aaaactacgc
      961 tttagtagct taataacgct gatagtgcta gtgtagatcg ctactagaga aagaggagaa
     1021 atactagatg tctgcccgta tttcgcgtaa ggaaatccat tatgtactat ttaaaaaaca
     1081 caaacttttg gatgttcggt ttattctttt tcttttactt ttttatcatg ggagcctact
     1141 tcccgttttt cccgatttgg ctacatgaca tcaaccatat cagcaaaagt gatacgggta
     1201 ttatttttgc cgctatttct ctgttctcgc tattattcca accgctgttt ggtctgcttt
     1261 ctgacaaact cgggctgcgc aaatacctgc tgtggattat taccggcatg ttagtgatgt
     1321 ttgcgccgtt ctttattttt atcttcgggc cactgttaca atacaacatt ttagtaggat
     1381 cgattgttgg tggtatttat ctaggctttt gttttaacgc cggtgcgcca gcagtagagg
     1441 catttattga gaaagtcagc cgtcgcagta atttcgaatt tggtcgcgcg cggatgtttg
     1501 gctgtgttgg ctgggcgctg tgtgcctcga ttgtcggcat catgttcacc atcaataatc
     1561 agtttgtttt ctggctgggc tctggctgtg cactcatcct cgccgtttta ctctttttcg
     1621 ccaaaacgga tgcgccctct tctgccacgg ttgccaatgc ggtaggtgcc aaccattcgg
     1681 catttagcct taagctggca ctggaactgt tcagacagcc aaaactgtgg tttttgtcac
     1741 tgtatgttat tggcgtttcc tgcacctacg atgtttttga ccaacagttt gctaatttct
     1801 ttacttcgtt ctttgctacc ggtgaacagg gtacgcgggt atttggctac gtaacgacaa
     1861 tgggcgaatt acttaacgcc tcgattatgt tctttgcgcc actgatcatt aatcgcatcg
     1921 gtgggaaaaa cgccctgctg ctggctggca ctattatgtc tgtacgtatt attggctcat
     1981 cgttcgccac ctcagcgctg gaagtggtta ttctgaaaac gctgcatatg tttgaagtac
     2041 cgttcctgct ggtgggctgc tttaaatata ttaccagcca gtttgaagtg cgtttttcag
     2101 cgacgattta tctggtctgt ttctgcttct ttaagcaact ggcgatgatt tttatgtctg
     2161 tactggcggg caatatgtat gaaagcatcg gtttccaggg cgcttatctg gtgctgggtc
     2221 tggtggcgct gggcttcacc ttaatttccg tgttcacgct tagcggcccc ggcccgcttt
     2281 ccctgctgcg tcgtcaggtg aatgaagtcg cttaatacta gagaaagagg agaaatacta
     2341 gatgcgtaaa ggagaagaac ttttcactgg agttgtccca attcttgttg aattagatgg
     2401 tgatgttaat gggcacaaat tttctgtcag tggagagggt gaaggtgatg caacatacgg
     2461 aaaacttacc cttaaattta tttgcactac tggaaaacta cctgttccat ggccaacact
     2521 tgtcactact ttcggttatg gtgttcaatg ctttgcgaga tacccagatc atatgaaaca
     2581 gcatgacttt ttcaagagtg ccatgcccga aggttatgta caggaaagaa ctatattttt
     2641 caaagatgac gggaactaca agacacgtgc tgaagtcaag tttgaaggtg atacccttgt
     2701 taatagaatc gagttaaaag gtattgattt taaagaagat ggaaacattc ttggacacaa
     2761 attggaatac aactataact cacacaatgt atacatcatg gcagacaaac aaaagaatgg
     2821 aatcaaagtt aacttcaaaa ttagacacaa cattgaagat ggaagcgttc aactagcaga
     2881 ccattatcaa caaaatactc caattggcga tggccctgtc cttttaccag acaaccatta
     2941 cctgtccaca caatctgccc tttcgaaaga tcccaacgaa aagagagacc acatggtcct
     3001 tcttgagttt gtaacagctg ctgggattac acatggcatg gatgaactat acaaaaggcc
     3061 tgctgcaaac gacgaaaact acgctttagt agcttaataa tactagagcc aggcatcaaa
     3121 taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga
     3181 acgctctcta ctagagtcac actggctcac cttcgggtgg gcctttctgc gtttata
//