LOCUS BBa_I763029 5541 bp DNA linear 22-Dec-2024 DEFINITION Genetic Schmitt Trigger ACCESSION BBa_I763029 VERSION BBa_I763029.1 FEATURES Location/Qualifiers misc_feature 1..19 /label=TetR 1 misc_feature 1..54 /label=BBa_R0040 promoter 20..25 /label=-35 misc_feature 26..44 /label=TetR 2 promoter 43..48 /label=-10 promoter 1..54 /label=BBa_R0040 misc_feature 67..70 /label=conserved RBS 63..74 /label=BBa_B0034 misc_feature 1170..1208 /label=LVA CDS 81..1208 /label=lacI-LVA misc_feature 81..1208 /label=BBa_C0012 misc_feature 1209..1233 /label=Help:Barcodes CDS 81..1208 /label=BBa_C0012 misc_feature 63..1233 /label=BBa_S0100 misc_feature 1..1233 /label=BBa_I763025 stem_loop 1253..1296 /label=stem_loop misc_feature 1242..1321 /label=BBa_B0010 terminator 1242..1321 /label=BBa_B0010 stem_loop 1337..1356 /label=T7 TE misc_feature 1363..1363 /label=stop polyA_site 1357..1370 /label=polya misc_feature 1330..1370 /label=BBa_B0012 terminator 1330..1370 /label=BBa_B0012 terminator 1242..1370 /label=BBa_B0015 misc_feature 1..1370 /label=BBa_I763026 CDS 1379..1466 /label=end of LacI coding region (inactive) misc_feature 1379..1578 /label=BBa_R0010 misc_feature 1467..1504 /label=CAP binding site promoter 1515..1520 /label=-35 promoter 1539..1544 /label=-10 misc_feature 1544..1578 /label=LacI binding site misc_feature 1551..1551 /label=start promoter 1379..1578 /label=BBa_R0010 misc_feature 1591..1594 /label=conserved RBS 1587..1598 /label=BBa_B0034 misc_feature 1379..1598 /label=BBa_J04500 CDS 1608..2315 /label=cI lambda misc_feature 2316..2348 /label=LVA misc_feature 2355..2379 /label=Help:Barcodes CDS 1605..2379 /label=BBa_C0051 misc_feature 1379..2379 /label=BBa_I763005 misc_feature 2392..2395 /label=conserved RBS 2388..2399 /label=BBa_B0034 CDS 2406..3693 /label=BBa_J22101 misc_feature 2388..3693 /label=BBa_I763015 misc_feature 1379..3693 /label=BBa_I763016 misc_feature 3706..3709 /label=conserved RBS 3702..3713 /label=BBa_B0034 misc_feature 3720..4439 /label=BBa_E0040 misc_feature 4434..4472 /label=LVA misc_feature 4473..4478 /label=Double Stop misc_feature 3720..3722 /label=Start misc_feature 3720..4478 /label=BBa_J04031 stem_loop 4498..4541 /label=stem_loop misc_feature 4487..4566 /label=BBa_B0010 terminator 4487..4566 /label=BBa_B0010 stem_loop 4582..4601 /label=T7 TE misc_feature 4608..4608 /label=stop polyA_site 4602..4615 /label=polya misc_feature 4575..4615 /label=BBa_B0012 terminator 4575..4615 /label=BBa_B0012 misc_feature 3702..4615 /label=BBa_I763020 misc_feature 1379..4615 /label=BBa_I763019 misc_feature 1..4615 /label=BBa_I763028 promoter 4661..4666 /label=-10 promoter 4638..4643 /label=-35 misc_feature 4648..4664 /label=OR1 misc_feature 4624..4640 /label=OR2 misc_feature 4624..4672 /label=BBa_R0051 promoter 4624..4672 /label=BBa_R0051 misc_feature 4685..4688 /label=conserved RBS 4681..4692 /label=BBa_B0034 CDS 4699..5373 /label=mrfp1 misc_feature 5380..5404 /label=Help:Barcodes CDS 4699..5379 /label=BBa_E1010 stem_loop 5424..5467 /label=stem_loop misc_feature 5413..5492 /label=BBa_B0010 terminator 5413..5492 /label=BBa_B0010 stem_loop 5508..5527 /label=T7 TE misc_feature 5534..5534 /label=stop polyA_site 5528..5541 /label=polya misc_feature 5501..5541 /label=BBa_B0012 terminator 5501..5541 /label=BBa_B0012 misc_feature 4681..5541 /label=BBa_I13507 misc_feature 4624..5541 /label=BBa_I763007 ORIGIN 1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag 61 agaaagagga gaaatactag atggtgaatg tgaaaccagt aacgttatac gatgtcgcag 121 agtatgccgg tgtctcttat cagaccgttt cccgcgtggt gaaccaggcc agccacgttt 181 ctgcgaaaac gcgggaaaaa gtggaagcgg cgatggcgga gctgaattac attcccaacc 241 gcgtggcaca acaactggcg ggcaaacagt cgttgctgat tggcgttgcc acctccagtc 301 tggccctgca cgcgccgtcg caaattgtcg cggcgattaa atctcgcgcc gatcaactgg 361 gtgccagcgt ggtggtgtcg atggtagaac gaagcggcgt cgaagcctgt aaagcggcgg 421 tgcacaatct tctcgcgcaa cgcgtcagtg ggctgatcat taactatccg ctggatgacc 481 aggatgccat tgctgtggaa gctgcctgca ctaatgttcc ggcgttattt cttgatgtct 541 ctgaccagac acccatcaac agtattattt tctcccatga agacggtacg cgactgggcg 601 tggagcatct ggtcgcattg ggtcaccagc aaatcgcgct gttagcgggc ccattaagtt 661 ctgtctcggc gcgtctgcgt ctggctggct ggcataaata tctcactcgc aatcaaattc 721 agccgatagc ggaacgggaa ggcgactgga gtgccatgtc cggttttcaa caaaccatgc 781 aaatgctgaa tgagggcatc gttcccactg cgatgctggt tgccaacgat cagatggcgc 841 tgggcgcaat gcgcgccatt accgagtccg ggctgcgcgt tggtgcggat atctcggtag 901 tgggatacga cgataccgaa gacagctcat gttatatccc gccgttaacc accatcaaac 961 aggattttcg cctgctgggg caaaccagcg tggaccgctt gctgcaactc tctcagggcc 1021 aggcggtgaa gggcaatcag ctgttgcccg tctcactggt gaaaagaaaa accaccctgg 1081 cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac 1141 gacaggtttc ccgactggaa agcgggcagg ctgcaaacga cgaaaactac gctttagtag 1201 cttaataact ctgatagtgc tagtgtagat ctctactaga gccaggcatc aaataaaacg 1261 aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct 1321 ctactagagt cacactggct caccttcggg tgggcctttc tgcgtttata tactagagca 1381 atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg 1441 tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat 1501 taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc 1561 ggataacaat ttcacacata ctagagaaag aggagaaata ctagatgagc acaaaaaaga 1621 aaccattaac acaagagcag cttgaggacg cacgtcgcct taaagcaatt tatgaaaaaa 1681 agaaaaatga acttggctta tcccaggaat ctgtcgcaga caagatgggg atggggcagt 1741 caggcgttgg tgctttattt aatggcatca atgcattaaa tgcttataac gccgcattgc 1801 ttgcaaaaat tctcaaagtt agcgttgaag aatttagccc ttcaatcgcc agagaaatct 1861 acgagatgta tgaagcggtt agtatgcagc cgtcacttag aagtgagtat gagtaccctg 1921 ttttttctca tgttcaggca gggatgttct cacctgagct tagaaccttt accaaaggtg 1981 atgcggagag atgggtaagc acaaccaaaa aagccagtga ttctgcattc tggcttgagg 2041 ttgaaggtaa ttccatgacc gcaccaacag gctccaagcc aagctttcct gacggaatgt 2101 taattctcgt tgaccctgag caggctgttg agccaggtga tttctgcata gccagacttg 2161 ggggtgatga gtttaccttc aagaaactga tcagggatag cggtcaggtg tttttacaac 2221 cactaaaccc acagtaccca atgatcccat gcaatgagag ttgttccgtt gtggggaaag 2281 ttatcgctag tcagtggcct gaagagacgt ttggcgctgc aaacgacgaa aactacgctt 2341 tagtagctta ataacgctga tagtgctagt gtagatcgct actagagaaa gaggagaaat 2401 actagatgtc tgcccgtatt tcgcgtaagg aaatccatta tgtactattt aaaaaacaca 2461 aacttttgga tgttcggttt attctttttc ttttactttt ttatcatggg agcctacttc 2521 ccgtttttcc cgatttggct acatgacatc aaccatatca gcaaaagtga tacgggtatt 2581 atttttgccg ctatttctct gttctcgcta ttattccaac cgctgtttgg tctgctttct 2641 gacaaactcg ggctgcgcaa atacctgctg tggattatta ccggcatgtt agtgatgttt 2701 gcgccgttct ttatttttat cttcgggcca ctgttacaat acaacatttt agtaggatcg 2761 attgttggtg gtatttatct aggcttttgt tttaacgccg gtgcgccagc agtagaggca 2821 tttattgaga aagtcagccg tcgcagtaat ttcgaatttg gtcgcgcgcg gatgtttggc 2881 tgtgttggct gggcgctgtg tgcctcgatt gtcggcatca tgttcaccat caataatcag 2941 tttgttttct ggctgggctc tggctgtgca ctcatcctcg ccgttttact ctttttcgcc 3001 aaaacggatg cgccctcttc tgccacggtt gccaatgcgg taggtgccaa ccattcggca 3061 tttagcctta agctggcact ggaactgttc agacagccaa aactgtggtt tttgtcactg 3121 tatgttattg gcgtttcctg cacctacgat gtttttgacc aacagtttgc taatttcttt 3181 acttcgttct ttgctaccgg tgaacagggt acgcgggtat ttggctacgt aacgacaatg 3241 ggcgaattac ttaacgcctc gattatgttc tttgcgccac tgatcattaa tcgcatcggt 3301 gggaaaaacg ccctgctgct ggctggcact attatgtctg tacgtattat tggctcatcg 3361 ttcgccacct cagcgctgga agtggttatt ctgaaaacgc tgcatatgtt tgaagtaccg 3421 ttcctgctgg tgggctgctt taaatatatt accagccagt ttgaagtgcg tttttcagcg 3481 acgatttatc tggtctgttt ctgcttcttt aagcaactgg cgatgatttt tatgtctgta 3541 ctggcgggca atatgtatga aagcatcggt ttccagggcg cttatctggt gctgggtctg 3601 gtggcgctgg gcttcacctt aatttccgtg ttcacgctta gcggccccgg cccgctttcc 3661 ctgctgcgtc gtcaggtgaa tgaagtcgct taatactaga gaaagaggag aaatactaga 3721 tgcgtaaagg agaagaactt ttcactggag ttgtcccaat tcttgttgaa ttagatggtg 3781 atgttaatgg gcacaaattt tctgtcagtg gagagggtga aggtgatgca acatacggaa 3841 aacttaccct taaatttatt tgcactactg gaaaactacc tgttccatgg ccaacacttg 3901 tcactacttt cggttatggt gttcaatgct ttgcgagata cccagatcat atgaaacagc 3961 atgacttttt caagagtgcc atgcccgaag gttatgtaca ggaaagaact atatttttca 4021 aagatgacgg gaactacaag acacgtgctg aagtcaagtt tgaaggtgat acccttgtta 4081 atagaatcga gttaaaaggt attgatttta aagaagatgg aaacattctt ggacacaaat 4141 tggaatacaa ctataactca cacaatgtat acatcatggc agacaaacaa aagaatggaa 4201 tcaaagttaa cttcaaaatt agacacaaca ttgaagatgg aagcgttcaa ctagcagacc 4261 attatcaaca aaatactcca attggcgatg gccctgtcct tttaccagac aaccattacc 4321 tgtccacaca atctgccctt tcgaaagatc ccaacgaaaa gagagaccac atggtccttc 4381 ttgagtttgt aacagctgct gggattacac atggcatgga tgaactatac aaaaggcctg 4441 ctgcaaacga cgaaaactac gctttagtag cttaataata ctagagccag gcatcaaata 4501 aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac 4561 gctctctact agagtcacac tggctcacct tcgggtgggc ctttctgcgt ttatatacta 4621 gagtaacacc gtgcgtgttg actattttac ctctggcggt gataatggtt gctactagag 4681 aaagaggaga aatactagat ggcttcctcc gaagacgtta tcaaagagtt catgcgtttc 4741 aaagttcgta tggaaggttc cgttaacggt cacgagttcg aaatcgaagg tgaaggtgaa 4801 ggtcgtccgt acgaaggtac ccagaccgct aaactgaaag ttaccaaagg tggtccgctg 4861 ccgttcgctt gggacatcct gtccccgcag ttccagtacg gttccaaagc ttacgttaaa 4921 cacccggctg acatcccgga ctacctgaaa ctgtccttcc cggaaggttt caaatgggaa 4981 cgtgttatga acttcgaaga cggtggtgtt gttaccgtta cccaggactc ctccctgcaa 5041 gacggtgagt tcatctacaa agttaaactg cgtggtacca acttcccgtc cgacggtccg 5101 gttatgcaga aaaaaaccat gggttgggaa gcttccaccg aacgtatgta cccggaagac 5161 ggtgctctga aaggtgaaat caaaatgcgt ctgaaactga aagacggtgg tcactacgac 5221 gctgaagtta aaaccaccta catggctaaa aaaccggttc agctgccggg tgcttacaaa 5281 accgacatca aactggacat cacctcccac aacgaagact acaccatcgt tgaacagtac 5341 gaacgtgctg aaggtcgtca ctccaccggt gcttaataac gctgatagtg ctagtgtaga 5401 tcgctactag agccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc 5461 gttttatctg ttgtttgtcg gtgaacgctc tctactagag tcacactggc tcaccttcgg 5521 gtgggccttt ctgcgtttat a //