LOCUS       BBa_I763031             1923 bp    DNA     linear       01-Jun-2024
DEFINITION  cI coding device switched on by IPTG with GFP as a reporter
ACCESSION   BBa_I763031
VERSION     BBa_I763031.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    213..216
                     /label=conserved
     RBS             209..220
                     /label=BBa_B0034
     misc_feature    1..220
                     /label=BBa_J04500
     CDS             230..937
                     /label=cI lambda
     misc_feature    938..970
                     /label=LVA
     misc_feature    977..1001
                     /label=Help:Barcodes
     CDS             227..1001
                     /label=BBa_C0051
     misc_feature    1..1001
                     /label=BBa_I763005
     misc_feature    1014..1017
                     /label=conserved
     RBS             1010..1021
                     /label=BBa_B0034
     misc_feature    1028..1747
                     /label=BBa_E0040
     misc_feature    1742..1780
                     /label=LVA
     misc_feature    1781..1786
                     /label=Double Stop
     misc_feature    1028..1030
                     /label=Start
     misc_feature    1028..1786
                     /label=BBa_J04031
     stem_loop       1806..1849
                     /label=stem_loop
     misc_feature    1795..1874
                     /label=BBa_B0010
     terminator      1795..1874
                     /label=BBa_B0010
     stem_loop       1890..1909
                     /label=T7 TE
     misc_feature    1916..1916
                     /label=stop
     polyA_site      1910..1923
                     /label=polya
     misc_feature    1883..1923
                     /label=BBa_B0012
     terminator      1883..1923
                     /label=BBa_B0012
     misc_feature    1010..1923
                     /label=BBa_I763020
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatga gcacaaaaaa
      241 gaaaccatta acacaagagc agcttgagga cgcacgtcgc cttaaagcaa tttatgaaaa
      301 aaagaaaaat gaacttggct tatcccagga atctgtcgca gacaagatgg ggatggggca
      361 gtcaggcgtt ggtgctttat ttaatggcat caatgcatta aatgcttata acgccgcatt
      421 gcttgcaaaa attctcaaag ttagcgttga agaatttagc ccttcaatcg ccagagaaat
      481 ctacgagatg tatgaagcgg ttagtatgca gccgtcactt agaagtgagt atgagtaccc
      541 tgttttttct catgttcagg cagggatgtt ctcacctgag cttagaacct ttaccaaagg
      601 tgatgcggag agatgggtaa gcacaaccaa aaaagccagt gattctgcat tctggcttga
      661 ggttgaaggt aattccatga ccgcaccaac aggctccaag ccaagctttc ctgacggaat
      721 gttaattctc gttgaccctg agcaggctgt tgagccaggt gatttctgca tagccagact
      781 tgggggtgat gagtttacct tcaagaaact gatcagggat agcggtcagg tgtttttaca
      841 accactaaac ccacagtacc caatgatccc atgcaatgag agttgttccg ttgtggggaa
      901 agttatcgct agtcagtggc ctgaagagac gtttggcgct gcaaacgacg aaaactacgc
      961 tttagtagct taataacgct gatagtgcta gtgtagatcg ctactagaga aagaggagaa
     1021 atactagatg cgtaaaggag aagaactttt cactggagtt gtcccaattc ttgttgaatt
     1081 agatggtgat gttaatgggc acaaattttc tgtcagtgga gagggtgaag gtgatgcaac
     1141 atacggaaaa cttaccctta aatttatttg cactactgga aaactacctg ttccatggcc
     1201 aacacttgtc actactttcg gttatggtgt tcaatgcttt gcgagatacc cagatcatat
     1261 gaaacagcat gactttttca agagtgccat gcccgaaggt tatgtacagg aaagaactat
     1321 atttttcaaa gatgacggga actacaagac acgtgctgaa gtcaagtttg aaggtgatac
     1381 ccttgttaat agaatcgagt taaaaggtat tgattttaaa gaagatggaa acattcttgg
     1441 acacaaattg gaatacaact ataactcaca caatgtatac atcatggcag acaaacaaaa
     1501 gaatggaatc aaagttaact tcaaaattag acacaacatt gaagatggaa gcgttcaact
     1561 agcagaccat tatcaacaaa atactccaat tggcgatggc cctgtccttt taccagacaa
     1621 ccattacctg tccacacaat ctgccctttc gaaagatccc aacgaaaaga gagaccacat
     1681 ggtccttctt gagtttgtaa cagctgctgg gattacacat ggcatggatg aactatacaa
     1741 aaggcctgct gcaaacgacg aaaactacgc tttagtagct taataatact agagccaggc
     1801 atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt
     1861 cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct ttctgcgttt
     1921 ata
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