LOCUS       BBa_I763032             2436 bp    DNA     linear       22-Dec-2024
DEFINITION  LacY coding device switched on by IPTG with GFP as a reporter
ACCESSION   BBa_I763032
VERSION     BBa_I763032.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    213..216
                     /label=conserved
     RBS             209..220
                     /label=BBa_B0034
     CDS             227..1514
                     /label=BBa_J22101
     misc_feature    1527..1530
                     /label=conserved
     RBS             1523..1534
                     /label=BBa_B0034
     misc_feature    1541..2260
                     /label=BBa_E0040
     misc_feature    2255..2293
                     /label=LVA
     misc_feature    2294..2299
                     /label=Double Stop
     misc_feature    1541..1543
                     /label=Start
     misc_feature    1541..2299
                     /label=BBa_J04031
     stem_loop       2319..2362
                     /label=stem_loop
     misc_feature    2308..2387
                     /label=BBa_B0010
     terminator      2308..2387
                     /label=BBa_B0010
     stem_loop       2403..2422
                     /label=T7 TE
     misc_feature    2429..2429
                     /label=stop
     polyA_site      2423..2436
                     /label=polya
     misc_feature    2396..2436
                     /label=BBa_B0012
     terminator      2396..2436
                     /label=BBa_B0012
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatgt ctgcccgtat
      241 ttcgcgtaag gaaatccatt atgtactatt taaaaaacac aaacttttgg atgttcggtt
      301 tattcttttt cttttacttt tttatcatgg gagcctactt cccgtttttc ccgatttggc
      361 tacatgacat caaccatatc agcaaaagtg atacgggtat tatttttgcc gctatttctc
      421 tgttctcgct attattccaa ccgctgtttg gtctgctttc tgacaaactc gggctgcgca
      481 aatacctgct gtggattatt accggcatgt tagtgatgtt tgcgccgttc tttattttta
      541 tcttcgggcc actgttacaa tacaacattt tagtaggatc gattgttggt ggtatttatc
      601 taggcttttg ttttaacgcc ggtgcgccag cagtagaggc atttattgag aaagtcagcc
      661 gtcgcagtaa tttcgaattt ggtcgcgcgc ggatgtttgg ctgtgttggc tgggcgctgt
      721 gtgcctcgat tgtcggcatc atgttcacca tcaataatca gtttgttttc tggctgggct
      781 ctggctgtgc actcatcctc gccgttttac tctttttcgc caaaacggat gcgccctctt
      841 ctgccacggt tgccaatgcg gtaggtgcca accattcggc atttagcctt aagctggcac
      901 tggaactgtt cagacagcca aaactgtggt ttttgtcact gtatgttatt ggcgtttcct
      961 gcacctacga tgtttttgac caacagtttg ctaatttctt tacttcgttc tttgctaccg
     1021 gtgaacaggg tacgcgggta tttggctacg taacgacaat gggcgaatta cttaacgcct
     1081 cgattatgtt ctttgcgcca ctgatcatta atcgcatcgg tgggaaaaac gccctgctgc
     1141 tggctggcac tattatgtct gtacgtatta ttggctcatc gttcgccacc tcagcgctgg
     1201 aagtggttat tctgaaaacg ctgcatatgt ttgaagtacc gttcctgctg gtgggctgct
     1261 ttaaatatat taccagccag tttgaagtgc gtttttcagc gacgatttat ctggtctgtt
     1321 tctgcttctt taagcaactg gcgatgattt ttatgtctgt actggcgggc aatatgtatg
     1381 aaagcatcgg tttccagggc gcttatctgg tgctgggtct ggtggcgctg ggcttcacct
     1441 taatttccgt gttcacgctt agcggccccg gcccgctttc cctgctgcgt cgtcaggtga
     1501 atgaagtcgc ttaatactag agaaagagga gaaatactag atgcgtaaag gagaagaact
     1561 tttcactgga gttgtcccaa ttcttgttga attagatggt gatgttaatg ggcacaaatt
     1621 ttctgtcagt ggagagggtg aaggtgatgc aacatacgga aaacttaccc ttaaatttat
     1681 ttgcactact ggaaaactac ctgttccatg gccaacactt gtcactactt tcggttatgg
     1741 tgttcaatgc tttgcgagat acccagatca tatgaaacag catgactttt tcaagagtgc
     1801 catgcccgaa ggttatgtac aggaaagaac tatatttttc aaagatgacg ggaactacaa
     1861 gacacgtgct gaagtcaagt ttgaaggtga tacccttgtt aatagaatcg agttaaaagg
     1921 tattgatttt aaagaagatg gaaacattct tggacacaaa ttggaataca actataactc
     1981 acacaatgta tacatcatgg cagacaaaca aaagaatgga atcaaagtta acttcaaaat
     2041 tagacacaac attgaagatg gaagcgttca actagcagac cattatcaac aaaatactcc
     2101 aattggcgat ggccctgtcc ttttaccaga caaccattac ctgtccacac aatctgccct
     2161 ttcgaaagat cccaacgaaa agagagacca catggtcctt cttgagtttg taacagctgc
     2221 tgggattaca catggcatgg atgaactata caaaaggcct gctgcaaacg acgaaaacta
     2281 cgctttagta gcttaataat actagagcca ggcatcaaat aaaacgaaag gctcagtcga
     2341 aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac tagagtcaca
     2401 ctggctcacc ttcgggtggg cctttctgcg tttata
//