LOCUS       BBa_I763035             2155 bp    DNA     linear       22-Dec-2024
DEFINITION  LacI coding device regulated by pTetR with GFP as a reporter
ACCESSION   BBa_I763035
VERSION     BBa_I763035.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    67..70
                     /label=conserved
     RBS             63..74
                     /label=BBa_B0034
     misc_feature    1170..1208
                     /label=LVA
     CDS             81..1208
                     /label=lacI-LVA
     misc_feature    81..1208
                     /label=BBa_C0012
     misc_feature    1209..1233
                     /label=Help:Barcodes
     CDS             81..1208
                     /label=BBa_C0012
     misc_feature    63..1233
                     /label=BBa_S0100
     misc_feature    1..1233
                     /label=BBa_I763025
     misc_feature    1246..1249
                     /label=conserved
     RBS             1242..1253
                     /label=BBa_B0034
     misc_feature    1260..1979
                     /label=BBa_E0040
     misc_feature    1974..2012
                     /label=LVA
     misc_feature    2013..2018
                     /label=Double Stop
     misc_feature    1260..1262
                     /label=Start
     misc_feature    1260..2018
                     /label=BBa_J04031
     stem_loop       2038..2081
                     /label=stem_loop
     misc_feature    2027..2106
                     /label=BBa_B0010
     terminator      2027..2106
                     /label=BBa_B0010
     stem_loop       2122..2141
                     /label=T7 TE
     misc_feature    2148..2148
                     /label=stop
     polyA_site      2142..2155
                     /label=polya
     misc_feature    2115..2155
                     /label=BBa_B0012
     terminator      2115..2155
                     /label=BBa_B0012
     misc_feature    1242..2155
                     /label=BBa_I763020
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agaaagagga gaaatactag atggtgaatg tgaaaccagt aacgttatac gatgtcgcag
      121 agtatgccgg tgtctcttat cagaccgttt cccgcgtggt gaaccaggcc agccacgttt
      181 ctgcgaaaac gcgggaaaaa gtggaagcgg cgatggcgga gctgaattac attcccaacc
      241 gcgtggcaca acaactggcg ggcaaacagt cgttgctgat tggcgttgcc acctccagtc
      301 tggccctgca cgcgccgtcg caaattgtcg cggcgattaa atctcgcgcc gatcaactgg
      361 gtgccagcgt ggtggtgtcg atggtagaac gaagcggcgt cgaagcctgt aaagcggcgg
      421 tgcacaatct tctcgcgcaa cgcgtcagtg ggctgatcat taactatccg ctggatgacc
      481 aggatgccat tgctgtggaa gctgcctgca ctaatgttcc ggcgttattt cttgatgtct
      541 ctgaccagac acccatcaac agtattattt tctcccatga agacggtacg cgactgggcg
      601 tggagcatct ggtcgcattg ggtcaccagc aaatcgcgct gttagcgggc ccattaagtt
      661 ctgtctcggc gcgtctgcgt ctggctggct ggcataaata tctcactcgc aatcaaattc
      721 agccgatagc ggaacgggaa ggcgactgga gtgccatgtc cggttttcaa caaaccatgc
      781 aaatgctgaa tgagggcatc gttcccactg cgatgctggt tgccaacgat cagatggcgc
      841 tgggcgcaat gcgcgccatt accgagtccg ggctgcgcgt tggtgcggat atctcggtag
      901 tgggatacga cgataccgaa gacagctcat gttatatccc gccgttaacc accatcaaac
      961 aggattttcg cctgctgggg caaaccagcg tggaccgctt gctgcaactc tctcagggcc
     1021 aggcggtgaa gggcaatcag ctgttgcccg tctcactggt gaaaagaaaa accaccctgg
     1081 cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac
     1141 gacaggtttc ccgactggaa agcgggcagg ctgcaaacga cgaaaactac gctttagtag
     1201 cttaataact ctgatagtgc tagtgtagat ctctactaga gaaagaggag aaatactaga
     1261 tgcgtaaagg agaagaactt ttcactggag ttgtcccaat tcttgttgaa ttagatggtg
     1321 atgttaatgg gcacaaattt tctgtcagtg gagagggtga aggtgatgca acatacggaa
     1381 aacttaccct taaatttatt tgcactactg gaaaactacc tgttccatgg ccaacacttg
     1441 tcactacttt cggttatggt gttcaatgct ttgcgagata cccagatcat atgaaacagc
     1501 atgacttttt caagagtgcc atgcccgaag gttatgtaca ggaaagaact atatttttca
     1561 aagatgacgg gaactacaag acacgtgctg aagtcaagtt tgaaggtgat acccttgtta
     1621 atagaatcga gttaaaaggt attgatttta aagaagatgg aaacattctt ggacacaaat
     1681 tggaatacaa ctataactca cacaatgtat acatcatggc agacaaacaa aagaatggaa
     1741 tcaaagttaa cttcaaaatt agacacaaca ttgaagatgg aagcgttcaa ctagcagacc
     1801 attatcaaca aaatactcca attggcgatg gccctgtcct tttaccagac aaccattacc
     1861 tgtccacaca atctgccctt tcgaaagatc ccaacgaaaa gagagaccac atggtccttc
     1921 ttgagtttgt aacagctgct gggattacac atggcatgga tgaactatac aaaaggcctg
     1981 ctgcaaacga cgaaaactac gctttagtag cttaataata ctagagccag gcatcaaata
     2041 aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac
     2101 gctctctact agagtcacac tggctcacct tcgggtgggc ctttctgcgt ttata
//