LOCUS       BBa_I763038             2282 bp    DNA     linear       23-Dec-2024
DEFINITION  -- No description --
ACCESSION   BBa_I763038
VERSION     BBa_I763038.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    213..216
                     /label=conserved
     RBS             209..220
                     /label=BBa_B0034
     misc_feature    233..236
                     /label=conserved
     RBS             229..240
                     /label=BBa_B0034
     misc_feature    1336..1374
                     /label=LVA
     CDS             247..1374
                     /label=lacI-LVA
     misc_feature    247..1374
                     /label=BBa_C0012
     misc_feature    1375..1399
                     /label=Help:Barcodes
     CDS             247..1374
                     /label=BBa_C0012
     misc_feature    1412..1415
                     /label=conserved
     RBS             1408..1419
                     /label=BBa_B0034
     CDS             1426..2145
                     /label=GFP protein
     CDS             1426..2145
                     /label=BBa_E0040
     stem_loop       2165..2208
                     /label=stem_loop
     misc_feature    2154..2233
                     /label=BBa_B0010
     terminator      2154..2233
                     /label=BBa_B0010
     stem_loop       2249..2268
                     /label=T7 TE
     misc_feature    2275..2275
                     /label=stop
     polyA_site      2269..2282
                     /label=polya
     misc_feature    2242..2282
                     /label=BBa_B0012
     terminator      2242..2282
                     /label=BBa_B0012
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagagaa agaggagaaa
      241 tactagatgg tgaatgtgaa accagtaacg ttatacgatg tcgcagagta tgccggtgtc
      301 tcttatcaga ccgtttcccg cgtggtgaac caggccagcc acgtttctgc gaaaacgcgg
      361 gaaaaagtgg aagcggcgat ggcggagctg aattacattc ccaaccgcgt ggcacaacaa
      421 ctggcgggca aacagtcgtt gctgattggc gttgccacct ccagtctggc cctgcacgcg
      481 ccgtcgcaaa ttgtcgcggc gattaaatct cgcgccgatc aactgggtgc cagcgtggtg
      541 gtgtcgatgg tagaacgaag cggcgtcgaa gcctgtaaag cggcggtgca caatcttctc
      601 gcgcaacgcg tcagtgggct gatcattaac tatccgctgg atgaccagga tgccattgct
      661 gtggaagctg cctgcactaa tgttccggcg ttatttcttg atgtctctga ccagacaccc
      721 atcaacagta ttattttctc ccatgaagac ggtacgcgac tgggcgtgga gcatctggtc
      781 gcattgggtc accagcaaat cgcgctgtta gcgggcccat taagttctgt ctcggcgcgt
      841 ctgcgtctgg ctggctggca taaatatctc actcgcaatc aaattcagcc gatagcggaa
      901 cgggaaggcg actggagtgc catgtccggt tttcaacaaa ccatgcaaat gctgaatgag
      961 ggcatcgttc ccactgcgat gctggttgcc aacgatcaga tggcgctggg cgcaatgcgc
     1021 gccattaccg agtccgggct gcgcgttggt gcggatatct cggtagtggg atacgacgat
     1081 accgaagaca gctcatgtta tatcccgccg ttaaccacca tcaaacagga ttttcgcctg
     1141 ctggggcaaa ccagcgtgga ccgcttgctg caactctctc agggccaggc ggtgaagggc
     1201 aatcagctgt tgcccgtctc actggtgaaa agaaaaacca ccctggcgcc caatacgcaa
     1261 accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga
     1321 ctggaaagcg ggcaggctgc aaacgacgaa aactacgctt tagtagctta ataactctga
     1381 tagtgctagt gtagatctct actagagaaa gaggagaaat actagatgcg taaaggagaa
     1441 gaacttttca ctggagttgt cccaattctt gttgaattag atggtgatgt taatgggcac
     1501 aaattttctg tcagtggaga gggtgaaggt gatgcaacat acggaaaact tacccttaaa
     1561 tttatttgca ctactggaaa actacctgtt ccatggccaa cacttgtcac tactttcggt
     1621 tatggtgttc aatgctttgc gagataccca gatcatatga aacagcatga ctttttcaag
     1681 agtgccatgc ccgaaggtta tgtacaggaa agaactatat ttttcaaaga tgacgggaac
     1741 tacaagacac gtgctgaagt caagtttgaa ggtgataccc ttgttaatag aatcgagtta
     1801 aaaggtattg attttaaaga agatggaaac attcttggac acaaattgga atacaactat
     1861 aactcacaca atgtatacat catggcagac aaacaaaaga atggaatcaa agttaacttc
     1921 aaaattagac acaacattga agatggaagc gttcaactag cagaccatta tcaacaaaat
     1981 actccaattg gcgatggccc tgtcctttta ccagacaacc attacctgtc cacacaatct
     2041 gccctttcga aagatcccaa cgaaaagaga gaccacatgg tccttcttga gtttgtaaca
     2101 gctgctggga ttacacatgg catggatgaa ctatacaaat aataatacta gagccaggca
     2161 tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc
     2221 ggtgaacgct ctctactaga gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta
     2281 ta
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