LOCUS       BBa_I763039             2296 bp    DNA     linear       22-Nov-2024
DEFINITION  pTeTR-LacI-plamda-RFP
ACCESSION   BBa_I763039
VERSION     BBa_I763039.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    67..70
                     /label=conserved
     RBS             63..74
                     /label=BBa_B0034
     misc_feature    1170..1208
                     /label=LVA
     CDS             81..1208
                     /label=lacI-LVA
     misc_feature    81..1208
                     /label=BBa_C0012
     misc_feature    1209..1233
                     /label=Help:Barcodes
     CDS             81..1208
                     /label=BBa_C0012
     misc_feature    63..1233
                     /label=BBa_S0100
     misc_feature    1..1233
                     /label=BBa_I763025
     stem_loop       1253..1296
                     /label=stem_loop
     misc_feature    1242..1321
                     /label=BBa_B0010
     terminator      1242..1321
                     /label=BBa_B0010
     stem_loop       1337..1356
                     /label=T7 TE
     misc_feature    1363..1363
                     /label=stop
     polyA_site      1357..1370
                     /label=polya
     misc_feature    1330..1370
                     /label=BBa_B0012
     terminator      1330..1370
                     /label=BBa_B0012
     terminator      1242..1370
                     /label=BBa_B0015
     misc_feature    1..1370
                     /label=BBa_I763026
     promoter        1416..1421
                     /label=-10
     promoter        1393..1398
                     /label=-35
     misc_feature    1403..1419
                     /label=OR1
     misc_feature    1379..1395
                     /label=OR2
     misc_feature    1379..1427
                     /label=BBa_R0051
     promoter        1379..1427
                     /label=BBa_R0051
     misc_feature    1440..1443
                     /label=conserved
     RBS             1436..1447
                     /label=BBa_B0034
     CDS             1454..2128
                     /label=mrfp1
     misc_feature    2135..2159
                     /label=Help:Barcodes
     CDS             1454..2134
                     /label=BBa_E1010
     stem_loop       2179..2222
                     /label=stem_loop
     misc_feature    2168..2247
                     /label=BBa_B0010
     terminator      2168..2247
                     /label=BBa_B0010
     stem_loop       2263..2282
                     /label=T7 TE
     misc_feature    2289..2289
                     /label=stop
     polyA_site      2283..2296
                     /label=polya
     misc_feature    2256..2296
                     /label=BBa_B0012
     terminator      2256..2296
                     /label=BBa_B0012
     misc_feature    1436..2296
                     /label=BBa_I13507
     misc_feature    1379..2296
                     /label=BBa_I763007
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agaaagagga gaaatactag atggtgaatg tgaaaccagt aacgttatac gatgtcgcag
      121 agtatgccgg tgtctcttat cagaccgttt cccgcgtggt gaaccaggcc agccacgttt
      181 ctgcgaaaac gcgggaaaaa gtggaagcgg cgatggcgga gctgaattac attcccaacc
      241 gcgtggcaca acaactggcg ggcaaacagt cgttgctgat tggcgttgcc acctccagtc
      301 tggccctgca cgcgccgtcg caaattgtcg cggcgattaa atctcgcgcc gatcaactgg
      361 gtgccagcgt ggtggtgtcg atggtagaac gaagcggcgt cgaagcctgt aaagcggcgg
      421 tgcacaatct tctcgcgcaa cgcgtcagtg ggctgatcat taactatccg ctggatgacc
      481 aggatgccat tgctgtggaa gctgcctgca ctaatgttcc ggcgttattt cttgatgtct
      541 ctgaccagac acccatcaac agtattattt tctcccatga agacggtacg cgactgggcg
      601 tggagcatct ggtcgcattg ggtcaccagc aaatcgcgct gttagcgggc ccattaagtt
      661 ctgtctcggc gcgtctgcgt ctggctggct ggcataaata tctcactcgc aatcaaattc
      721 agccgatagc ggaacgggaa ggcgactgga gtgccatgtc cggttttcaa caaaccatgc
      781 aaatgctgaa tgagggcatc gttcccactg cgatgctggt tgccaacgat cagatggcgc
      841 tgggcgcaat gcgcgccatt accgagtccg ggctgcgcgt tggtgcggat atctcggtag
      901 tgggatacga cgataccgaa gacagctcat gttatatccc gccgttaacc accatcaaac
      961 aggattttcg cctgctgggg caaaccagcg tggaccgctt gctgcaactc tctcagggcc
     1021 aggcggtgaa gggcaatcag ctgttgcccg tctcactggt gaaaagaaaa accaccctgg
     1081 cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac
     1141 gacaggtttc ccgactggaa agcgggcagg ctgcaaacga cgaaaactac gctttagtag
     1201 cttaataact ctgatagtgc tagtgtagat ctctactaga gccaggcatc aaataaaacg
     1261 aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct
     1321 ctactagagt cacactggct caccttcggg tgggcctttc tgcgtttata tactagagta
     1381 acaccgtgcg tgttgactat tttacctctg gcggtgataa tggttgctac tagagaaaga
     1441 ggagaaatac tagatggctt cctccgaaga cgttatcaaa gagttcatgc gtttcaaagt
     1501 tcgtatggaa ggttccgtta acggtcacga gttcgaaatc gaaggtgaag gtgaaggtcg
     1561 tccgtacgaa ggtacccaga ccgctaaact gaaagttacc aaaggtggtc cgctgccgtt
     1621 cgcttgggac atcctgtccc cgcagttcca gtacggttcc aaagcttacg ttaaacaccc
     1681 ggctgacatc ccggactacc tgaaactgtc cttcccggaa ggtttcaaat gggaacgtgt
     1741 tatgaacttc gaagacggtg gtgttgttac cgttacccag gactcctccc tgcaagacgg
     1801 tgagttcatc tacaaagtta aactgcgtgg taccaacttc ccgtccgacg gtccggttat
     1861 gcagaaaaaa accatgggtt gggaagcttc caccgaacgt atgtacccgg aagacggtgc
     1921 tctgaaaggt gaaatcaaaa tgcgtctgaa actgaaagac ggtggtcact acgacgctga
     1981 agttaaaacc acctacatgg ctaaaaaacc ggttcagctg ccgggtgctt acaaaaccga
     2041 catcaaactg gacatcacct cccacaacga agactacacc atcgttgaac agtacgaacg
     2101 tgctgaaggt cgtcactcca ccggtgctta ataacgctga tagtgctagt gtagatcgct
     2161 actagagcca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt
     2221 atctgttgtt tgtcggtgaa cgctctctac tagagtcaca ctggctcacc ttcgggtggg
     2281 cctttctgcg tttata
//