LOCUS       BBa_I765008             1621 bp    DNA     linear       28-Jun-2024
DEFINITION  TonB Iron channel -  EYFP reporter
ACCESSION   BBa_I765008
VERSION     BBa_I765008.1  
FEATURES             Location/Qualifiers
     RBS             1..10
                     /label=rbs
     CDS             12..735
                     /label=protein
     CDS             1..735
                     /label=BBa_I765003
     misc_feature    748..751
                     /label=conserved
     RBS             744..755
                     /label=BBa_B0034
     CDS             762..1484
                     /label=BBa_E0030
     stem_loop       1504..1547
                     /label=stem_loop
     misc_feature    1493..1572
                     /label=BBa_B0010
     terminator      1493..1572
                     /label=BBa_B0010
     stem_loop       1588..1607
                     /label=T7 TE
     misc_feature    1614..1614
                     /label=stop
     polyA_site      1608..1621
                     /label=polya
     misc_feature    1581..1621
                     /label=BBa_B0012
     terminator      1581..1621
                     /label=BBa_B0012
ORIGIN
        1 atgacgaaaa cgcgctcgaa catggcgcgc tacggcacca gcctggccat cgtgctgggc
       61 gtgcacgtgg tcgccgtggt gctgacgctc aactggtcgg tgccccaggc catcgagctg
      121 ccgccggcag cgatgatggt cgagttggcg ccgttgccgg agcccgcgcc accgccaccg
      181 cccaaggccg cgcccaagcc accggcagag gtcgaggagc cgccgctgcc caagctggtg
      241 gaggccccca agccgaagat cgccatcgcc aagccgccca agcccaaggc caagccgcag
      301 ccgcccaagc ctgagaaaaa gcctgagccg ccgaaggacg aaccaccggc caaggacgat
      361 gtggcggata ccccgccaag caacgcgcag ccgcagaaat cggccgcacc ggcaccgagc
      421 atcgcctcca acagcaatgc cctgcccagc tggcagagcg acctgctgcg ccacctggcc
      481 aagtacaaga agtacccgga agacgctcgc cgtcgcggcc tgcagggcat caaccgcctg
      541 cgcttcgtgg tcgacgccga gggcaaggta gtctcgtact cgctggccgg aggctcgggc
      601 agcgcggcgc tggaccgggc gaccctggaa atgatccgtc gcgcaggctc cgtaccgaag
      661 ccgccagcgg agctgttgaa caatggcacg atcgaagtcg tggcgccgtt cgtctattcc
      721 ctggaccgac gctgatacta gagaaagagg agaaatacta gatggtgagc aagggcgagg
      781 agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca
      841 agttcagcgt gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt
      901 tcatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accttcggct
      961 acggcctgca atgcttcgcc cgctaccccg accacatgaa gctgcacgac ttcttcaagt
     1021 ccgccatgcc cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact
     1081 acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga
     1141 agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag tacaactaca
     1201 acagccacaa cgtctatatc atggccgaca agcagaagaa cggcatcaag gtgaacttca
     1261 agatccgcca caacatcgag gacggcagcg tgcagctcgc cgaccactac cagcagaaca
     1321 cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc taccagtccg
     1381 ccctgagcaa agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg
     1441 ccgccgggat cactctcggc atggacgagc tgtacaagta ataatactag agccaggcat
     1501 caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg
     1561 gtgaacgctc tctactagag tcacactggc tcaccttcgg gtgggccttt ctgcgtttat
     1621 a
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