BBa_B0034
1
BBa_B0034
RBS (Elowitz 1999) -- defines RBS efficiency
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
RBS based on Elowitz repressilator.
false
true
_1_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation23325
1
conserved
range23325
1
5
8
BBa_B0010
1
BBa_B0010
T1 from E. coli rrnB
2003-11-19T12:00:00Z
2015-08-31T04:07:20Z
Transcriptional terminator consisting of a 64 bp stem-loop.
false
false
_1_
0
24
7
In stock
false
true
Randy Rettberg
annotation7018
1
BBa_B0010
range7018
1
1
80
annotation4184
1
stem_loop
range4184
1
12
55
BBa_I765009
1
BBa_I765009
ExbB iron channel - EYFP reporter
2007-10-25T11:00:00Z
2015-08-31T04:08:09Z
Genomic sequence, subpart
ExbB production is measured by the fluorescence emited by EYFP
false
false
_118_
0
2267
9
It's complicated
false
None
false
Sandra Garcia and Alex Quintero
component1957992
1
BBa_B0012
component1957989
1
BBa_E0030
component1957988
1
BBa_B0034
component1957986
1
BBa_I765002
component1957990
1
BBa_B0010
annotation1957986
1
BBa_I765002
range1957986
1
1
735
annotation1957992
1
BBa_B0012
range1957992
1
1581
1621
annotation1957989
1
BBa_E0030
range1957989
1
762
1484
annotation1957990
1
BBa_B0010
range1957990
1
1493
1572
annotation1957988
1
BBa_B0034
range1957988
1
744
755
BBa_E0030
1
eyfp
enhanced yellow fluorescent protein derived from A. victoria GFP
2004-03-02T12:00:00Z
2015-08-31T04:07:25Z
Modificaitons to Clontech EYFP by Reshma Shetty
Released HQ 2013
-- No description --
false
false
_1_
0
24
7
In stock
false
true
Caitlin Conboy and Jennifer Braff
BBa_I765002
1
ExbB
ExbB membrane spanning protein in TonB-ExbB-ExbD complex [ Escherichia coli K12 ]
2007-10-25T11:00:00Z
2015-08-31T04:08:09Z
genomic sequence
this part codifies for a membran localized protein that transduces energy from the proton motive force to high-affinity receptors in the outer membrane. it is part of the complex composede by a TonB ExbB and ExbD
false
false
_118_
0
2267
9
It's complicated
false
this part has its original ribosomal binding site
false
iGEM Colombian Team 2007
annotation1957821
1
rbs
range1957821
1
1
15
annotation1957820
1
protein
range1957820
1
16
735
BBa_B0012
1
BBa_B0012
TE from coliphageT7
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
Released HQ 2013
Transcription terminator for the <i>E.coli</i> RNA polymerase.
false
false
_1_
0
24
7
In stock
false
<P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
false
Reshma Shetty
annotation1690
1
polya
range1690
1
28
41
annotation7020
1
BBa_B0012
range7020
1
1
41
annotation1686
1
T7 TE
range1686
1
8
27
annotation1687
1
stop
range1687
1
34
34
BBa_I765009_sequence
1
gtgggtaataatttaatgcagacggacctttccgtctggggtatgtatcagcacgccgatattgtcgttaagtgcgtgatgattgggcttattttggcctccgtagtcacctgggcaatcttcttcagtaagagcgtagagttcttcaatcagaagcgtcgccttaagcgcgagcagcaactgctggctgaagcgcgttccttaaaccaggccaacgatatcgccgctgattttggtagcaaaagcttaagcctgcatttgctcaatgaagcgcagaatgagctggaactgtcagaaggcagcgacgataacgaaggtattaaagaacgtaccagcttccgtctggagcgtcgggtcgccgcagtgggtcgtcaaatgggccgcggtaacggctatctggcaaccatcggcgcgatttctccgttcgttggtctgtttggtacggtctggggcatcatgaacagctttattggtatcgcgcaaacgcagaccactaacctggcagtcgttgcgccgggtatcgcagaagctctgttagcaacggcaatcggcctcgtggcagcgattcctgcggtcgttatctataacgtatttgcacgccagattggcggctttaaagcgatgctgggtgatgttgcagcgcaggtattgttgctgcaaagccgtgacctggatctggaagccagcgccgctgcgcatccggttcgtgtcgcacaaaaattacgcgcaggataatactagagaaagaggagaaatactagatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccttcggctacggcctgcaatgcttcgcccgctaccccgaccacatgaagctgcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagctaccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagtaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_B0010_sequence
1
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
BBa_I765002_sequence
1
gtgggtaataatttaatgcagacggacctttccgtctggggtatgtatcagcacgccgatattgtcgttaagtgcgtgatgattgggcttattttggcctccgtagtcacctgggcaatcttcttcagtaagagcgtagagttcttcaatcagaagcgtcgccttaagcgcgagcagcaactgctggctgaagcgcgttccttaaaccaggccaacgatatcgccgctgattttggtagcaaaagcttaagcctgcatttgctcaatgaagcgcagaatgagctggaactgtcagaaggcagcgacgataacgaaggtattaaagaacgtaccagcttccgtctggagcgtcgggtcgccgcagtgggtcgtcaaatgggccgcggtaacggctatctggcaaccatcggcgcgatttctccgttcgttggtctgtttggtacggtctggggcatcatgaacagctttattggtatcgcgcaaacgcagaccactaacctggcagtcgttgcgccgggtatcgcagaagctctgttagcaacggcaatcggcctcgtggcagcgattcctgcggtcgttatctataacgtatttgcacgccagattggcggctttaaagcgatgctgggtgatgttgcagcgcaggtattgttgctgcaaagccgtgacctggatctggaagccagcgccgctgcgcatccggttcgtgtcgcacaaaaattacgcgcaggataa
BBa_B0034_sequence
1
aaagaggagaaa
BBa_E0030_sequence
1
atggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccttcggctacggcctgcaatgcttcgcccgctaccccgaccacatgaagctgcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagctaccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagtaataa
BBa_B0012_sequence
1
tcacactggctcaccttcgggtgggcctttctgcgtttata
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z