BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation7018 1 BBa_B0010 range7018 1 1 80 annotation4184 1 stem_loop range4184 1 12 55 BBa_I765009 1 BBa_I765009 ExbB iron channel - EYFP reporter 2007-10-25T11:00:00Z 2015-08-31T04:08:09Z Genomic sequence, subpart ExbB production is measured by the fluorescence emited by EYFP false false _118_ 0 2267 9 It's complicated false None false Sandra Garcia and Alex Quintero component1957992 1 BBa_B0012 component1957989 1 BBa_E0030 component1957988 1 BBa_B0034 component1957986 1 BBa_I765002 component1957990 1 BBa_B0010 annotation1957986 1 BBa_I765002 range1957986 1 1 735 annotation1957992 1 BBa_B0012 range1957992 1 1581 1621 annotation1957989 1 BBa_E0030 range1957989 1 762 1484 annotation1957990 1 BBa_B0010 range1957990 1 1493 1572 annotation1957988 1 BBa_B0034 range1957988 1 744 755 BBa_E0030 1 eyfp enhanced yellow fluorescent protein derived from A. victoria GFP 2004-03-02T12:00:00Z 2015-08-31T04:07:25Z Modificaitons to Clontech EYFP by Reshma Shetty Released HQ 2013 -- No description -- false false _1_ 0 24 7 In stock false true Caitlin Conboy and Jennifer Braff BBa_I765002 1 ExbB ExbB membrane spanning protein in TonB-ExbB-ExbD complex [ Escherichia coli K12 ] 2007-10-25T11:00:00Z 2015-08-31T04:08:09Z genomic sequence this part codifies for a membran localized protein that transduces energy from the proton motive force to high-affinity receptors in the outer membrane. it is part of the complex composede by a TonB ExbB and ExbD false false _118_ 0 2267 9 It's complicated false this part has its original ribosomal binding site false iGEM Colombian Team 2007 annotation1957821 1 rbs range1957821 1 1 15 annotation1957820 1 protein range1957820 1 16 735 BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation1690 1 polya range1690 1 28 41 annotation7020 1 BBa_B0012 range7020 1 1 41 annotation1686 1 T7 TE range1686 1 8 27 annotation1687 1 stop range1687 1 34 34 BBa_I765009_sequence 1 gtgggtaataatttaatgcagacggacctttccgtctggggtatgtatcagcacgccgatattgtcgttaagtgcgtgatgattgggcttattttggcctccgtagtcacctgggcaatcttcttcagtaagagcgtagagttcttcaatcagaagcgtcgccttaagcgcgagcagcaactgctggctgaagcgcgttccttaaaccaggccaacgatatcgccgctgattttggtagcaaaagcttaagcctgcatttgctcaatgaagcgcagaatgagctggaactgtcagaaggcagcgacgataacgaaggtattaaagaacgtaccagcttccgtctggagcgtcgggtcgccgcagtgggtcgtcaaatgggccgcggtaacggctatctggcaaccatcggcgcgatttctccgttcgttggtctgtttggtacggtctggggcatcatgaacagctttattggtatcgcgcaaacgcagaccactaacctggcagtcgttgcgccgggtatcgcagaagctctgttagcaacggcaatcggcctcgtggcagcgattcctgcggtcgttatctataacgtatttgcacgccagattggcggctttaaagcgatgctgggtgatgttgcagcgcaggtattgttgctgcaaagccgtgacctggatctggaagccagcgccgctgcgcatccggttcgtgtcgcacaaaaattacgcgcaggataatactagagaaagaggagaaatactagatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccttcggctacggcctgcaatgcttcgcccgctaccccgaccacatgaagctgcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagctaccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagtaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_I765002_sequence 1 gtgggtaataatttaatgcagacggacctttccgtctggggtatgtatcagcacgccgatattgtcgttaagtgcgtgatgattgggcttattttggcctccgtagtcacctgggcaatcttcttcagtaagagcgtagagttcttcaatcagaagcgtcgccttaagcgcgagcagcaactgctggctgaagcgcgttccttaaaccaggccaacgatatcgccgctgattttggtagcaaaagcttaagcctgcatttgctcaatgaagcgcagaatgagctggaactgtcagaaggcagcgacgataacgaaggtattaaagaacgtaccagcttccgtctggagcgtcgggtcgccgcagtgggtcgtcaaatgggccgcggtaacggctatctggcaaccatcggcgcgatttctccgttcgttggtctgtttggtacggtctggggcatcatgaacagctttattggtatcgcgcaaacgcagaccactaacctggcagtcgttgcgccgggtatcgcagaagctctgttagcaacggcaatcggcctcgtggcagcgattcctgcggtcgttatctataacgtatttgcacgccagattggcggctttaaagcgatgctgggtgatgttgcagcgcaggtattgttgctgcaaagccgtgacctggatctggaagccagcgccgctgcgcatccggttcgtgtcgcacaaaaattacgcgcaggataa BBa_B0034_sequence 1 aaagaggagaaa BBa_E0030_sequence 1 atggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccttcggctacggcctgcaatgcttcgcccgctaccccgaccacatgaagctgcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagctaccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagtaataa BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z