BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation1686 1 T7 TE range1686 1 8 27 annotation1687 1 stop range1687 1 34 34 annotation1690 1 polya range1690 1 28 41 annotation7020 1 BBa_B0012 range7020 1 1 41 BBa_E0030 1 eyfp enhanced yellow fluorescent protein derived from A. victoria GFP 2004-03-02T12:00:00Z 2015-08-31T04:07:25Z Modificaitons to Clontech EYFP by Reshma Shetty Released HQ 2013 -- No description -- false false _1_ 0 24 7 In stock false true Caitlin Conboy and Jennifer Braff BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation4184 1 stem_loop range4184 1 12 55 annotation7018 1 BBa_B0010 range7018 1 1 80 BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_I765010 1 BBa_I765010 Iron promoter -EYFP reporter 2007-10-25T11:00:00Z 2015-08-31T04:08:09Z genomic sequense Iron promoter expretion is reported by the trascription of EYFP reporter false false _118_ 0 2267 9 It's complicated false this device comes from the subparts BBa_I765000 and BBa_E0430 false Juan David Lobaton and Alex Quintero component1958206 1 BBa_B0034 component1958207 1 BBa_E0030 component1958204 1 BBa_I765000 component1958208 1 BBa_B0010 component1958210 1 BBa_B0012 annotation1958207 1 BBa_E0030 range1958207 1 1071 1793 annotation1958206 1 BBa_B0034 range1958206 1 1053 1064 annotation1958204 1 BBa_I765000 range1958204 1 1 1044 annotation1958210 1 BBa_B0012 range1958210 1 1890 1930 annotation1958208 1 BBa_B0010 range1958208 1 1802 1881 BBa_I765000 1 pFe Fe promoter 2007-10-25T11:00:00Z 2015-08-31T04:08:09Z Genomic sequence Promoter regulated by iron presence false false _118_ 0 2266 9 It's complicated false None false Raul Cuero annotation1956037 1 promoter range1956037 1 1 1045 BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_B0034_sequence 1 aaagaggagaaa BBa_I765010_sequence 1 atgacgcttgtcgacagattgcgcggcgccgtggcggggatgccgcgccggctcgtggtgggggccgctggtgcggcgctgctctcgggcctgattggcgccgtcgggggctcggcgaccgccggggccttctcgcgccccggtctgccggtggagtacctgcaggttccctccgccgccatgggacgggacatcaaggtccagttccaaagcggtggggccaactcgcccgcgttgtacctgctcgacgggatgcgcgcgcaagacgacttcaacggctgggacatcaacaccccggcgttcgagtggtacaaccagtcgggcatctcggtcgccatgccggtcggcggccagtccagcttctactccgactggtacaagcccgcctgcggcaaggccggctgcaccacctacaagtgggagaccttcctgaccagcgagctgccgcagtacctgtcggcgcagaagcaggtcaagccgaccggcagcggtgtcgtcggcctgtcgatggccggctcctcggcgctgatcctggccgcctaccaccccgaccagttcgtctacgccggctcgctgtcggcgctgctggactcgtcgcagggcatgggcccgtcgctgatcgggctggccatgggtgacgccggtggctacaaggccgccgacatgtggggtccgaaggaggacccggcctgggcccgcaacgacccgtcgctgcaggtcggcaagctggtcgcgaacaacacccggatctgggtgtactgcggcaacggcaagccgtccgacctcggtggcgacaacctgcccgccaagttcctcgagggcttcgtgcggacgtccaacctgaagttccaggacgcctacaacggcgccggcggccacaacgcggtgtggaacttcgacgccaacggcacccacgactggccctactggggcgcgcagctgcaggcgatgaagcctgacctgcagtcggtgctgggcgccaccccgggcgccggtccggccacggccgcggccaccaatgctgggaacggccagggcacctaatactagagaaagaggagaaatactagatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccttcggctacggcctgcaatgcttcgcccgctaccccgaccacatgaagctgcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagctaccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagtaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_I765000_sequence 1 atgacgcttgtcgacagattgcgcggcgccgtggcggggatgccgcgccggctcgtggtgggggccgctggtgcggcgctgctctcgggcctgattggcgccgtcgggggctcggcgaccgccggggccttctcgcgccccggtctgccggtggagtacctgcaggttccctccgccgccatgggacgggacatcaaggtccagttccaaagcggtggggccaactcgcccgcgttgtacctgctcgacgggatgcgcgcgcaagacgacttcaacggctgggacatcaacaccccggcgttcgagtggtacaaccagtcgggcatctcggtcgccatgccggtcggcggccagtccagcttctactccgactggtacaagcccgcctgcggcaaggccggctgcaccacctacaagtgggagaccttcctgaccagcgagctgccgcagtacctgtcggcgcagaagcaggtcaagccgaccggcagcggtgtcgtcggcctgtcgatggccggctcctcggcgctgatcctggccgcctaccaccccgaccagttcgtctacgccggctcgctgtcggcgctgctggactcgtcgcagggcatgggcccgtcgctgatcgggctggccatgggtgacgccggtggctacaaggccgccgacatgtggggtccgaaggaggacccggcctgggcccgcaacgacccgtcgctgcaggtcggcaagctggtcgcgaacaacacccggatctgggtgtactgcggcaacggcaagccgtccgacctcggtggcgacaacctgcccgccaagttcctcgagggcttcgtgcggacgtccaacctgaagttccaggacgcctacaacggcgccggcggccacaacgcggtgtggaacttcgacgccaacggcacccacgactggccctactggggcgcgcagctgcaggcgatgaagcctgacctgcagtcggtgctgggcgccaccccgggcgccggtccggccacggccgcggccaccaatgctgggaacggccagggcacctaa BBa_E0030_sequence 1 atggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccttcggctacggcctgcaatgcttcgcccgctaccccgaccacatgaagctgcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagctaccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagtaataa BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z