WMTM1-EE under weak constitutive promoter
This is a composite device; see individual parts for source
This device expresses the MTM1 phosphatase from C. Elegans fused to an EE leucine zipper with the weak constitutive promoter Ste5.
used combinatorial cloning methods described in the UCSF iGEM 2007 wiki.
From the genome of S cerevisiae. http://db.yeastgenome.org/cgi-bin/locus.pl?locus=ste2
Start Codon + Xho site + Combinatorial Cloning A site
low expression, constitutive promoter in yeast
endogenous terminator for Adh (Alcohol dehydrogenase) in yeast
Worm MTMR1 (myotubularin-related phosphatidylinositol phosphatase). Used to generate phosphatidyl inositol 5 phosphate in our organelle proejct
Adaptor Region for "B" Site in Combinatorial Cloning Approach
Adh terminator derived from genomic sequence; other components are synthetic.
Adaptor used for non-pSte2 constructs; contains a stop codon, a BamHI site followed by the combinatorial D adaptor region, followed by Adh terminator
for more information on combinatorial cloning, see documentation on the 2007 UCSF iGEM wiki
binds with the complementary zipper, RR. Can be used to create binding interactions between desired elements through fusing the zipper pair to the appropriate genes.
not a standard biobrick. cloned using primers containing an adaptor region for combinatorial cloning using type IIs restriction enzymes.
Conversion of the iGEM parts registry to SBOL2.1