BBa_I766225 1 BBa_I766225 WMTM1-EE under weak constitutive promoter 2007-10-21T11:00:00Z 2015-08-31T04:08:09Z This is a composite device; see individual parts for source This device expresses the MTM1 phosphatase from C. Elegans fused to an EE leucine zipper with the weak constitutive promoter Ste5. false false _155_ 0 1931 9 It's complicated true used combinatorial cloning methods described in the UCSF iGEM 2007 wiki. false Nili Sommovilla component1952561 1 BBa_I766215 component1952560 1 BBa_I766557 component1952565 1 BBa_I766217 component1952564 1 BBa_I766102 component1952563 1 BBa_I766216 component1952562 1 BBa_I766203 annotation1952563 1 BBa_I766216 range1952563 1 2400 2411 annotation1952564 1 BBa_I766102 range1952564 1 2420 2581 annotation1952562 1 BBa_I766203 range1952562 1 631 2391 annotation1952561 1 BBa_I766215 range1952561 1 608 622 annotation1952565 1 BBa_I766217 range1952565 1 2590 2782 annotation1952560 1 BBa_I766557 range1952560 1 1 601 BBa_I766203 1 BBa_I766203 WMTM1 2007-10-21T11:00:00Z 2015-08-31T04:08:09Z from C. elegans genomic DNA Worm MTMR1 (myotubularin-related phosphatidylinositol phosphatase). Used to generate phosphatidyl inositol 5 phosphate in our organelle proejct false false _155_ 0 1931 9 Not in stock false none false Nili Sommovilla BBa_I766215 1 BBa_I766215 Translation Initiation Adaptor 2007-10-21T11:00:00Z 2015-08-31T04:08:09Z synthetic Start Codon + Xho site + Combinatorial Cloning A site false true _155_ 0 1931 9 Not in stock false none false Nili Sommovilla BBa_I766557 1 BBa_I766557 pSte5 (Weak) Promoter 2007-08-15T11:00:00Z 2015-08-31T04:08:10Z endogenous promoter for Sterile 5 low expression, constitutive promoter in yeast false false _155_ 0 1931 9 Not in stock false none false Nili Sommovilla BBa_I766217 1 BBa_I766217 Stop-D-BamHI-Adh Terminator Adaptor 2007-10-21T11:00:00Z 2015-08-31T04:08:09Z Adh terminator derived from genomic sequence; other components are synthetic. Adaptor used for non-pSte2 constructs; contains a stop codon, a BamHI site followed by the combinatorial D adaptor region, followed by Adh terminator false false _155_ 0 1931 9 Not in stock false for more information on combinatorial cloning, see documentation on the 2007 UCSF iGEM wiki false Nili Sommovilla BBa_I766102 1 BBa_I766102 EE Leucine Zipper 2007-08-08T11:00:00Z 2015-08-31T04:08:09Z to be determined binds with the complementary zipper, RR. Can be used to create binding interactions between desired elements through fusing the zipper pair to the appropriate genes. false false _155_ 0 1931 9 Not in stock false not a standard biobrick. cloned using primers containing an adaptor region for combinatorial cloning using type IIs restriction enzymes. false Nili Sommovilla BBa_I766216 1 BBa_I766216 Combinatorial Cloning B Site 2007-10-21T11:00:00Z 2015-08-31T04:08:09Z synthetic Adaptor Region for "B" Site in Combinatorial Cloning Approach false true _155_ 0 1931 9 Not in stock false none false Nili Sommovilla BBa_I766216_sequence 1 ggtagttcccta BBa_I766102_sequence 1 ggtagcgatggttccggtagcggttccggttctatcactattagggctgcttttttggaaaaagaaaatactgctttgagaactgaaattgctgaattggaaaaagaagttggtagatgtgaaaatattgtttctaaatatgaaactagatatggtccattg BBa_I766225_sequence 1 cacgaagtgactgacagaatactgacatcagctgatttctcatagagctgtttctctgataacacgttgtttgaacatcgacaagatgaaaatctagaagtatcaagtttcctttaaagggatatataacagattctaaaactgacagaaatatttcgagtgaagaagaagcgttaaatattggatctttccgcagttctactctgatacatttttgaagtaggagagtcatttagaaggcgtattgctcaatagtagaaagcaggcctgtgcacatgaattaattaaaaaatataaaggtagtgattagacgacacatgtccataggtaacctgtcataattttgaacaatttcccttcttttcttttttttttttgggtgcggcgatatgtagcttgttaatttacacatcatgtacttttctgcatcaaaatatgaaaggcgatagtagctaaagaaaataccgagaatttcctcgaaaagttgacgacaaaagaaaggcataaaaaagtaatttgaaaatattttaaaactgttttaacccatctagcatccgcgctaaaaaaggaagatacaggatacagcggaaacaacttttaatactagatgctcgagggagcttactagaggatgacagagggaacaatagtggagaagttggagaatttgccagttcatcaatgatacaagaatcaatagatctcaaattgttagccgcagaaagtcttatttggacagagaaaaatgtgacatacttcggaccacttggaaaatttccaggtaaaatcgtaattactaggtatcgaatggtttttctcgttggtgatggtggaaaaatgtatgagcaatggaagctcgatattccactcggacaagtgtctcggatagaaaaagttggtagaaagacgacgagtgttgccaaacgaggcgatgataattatggattcacaatttattgtaaagattatcgagtttatcgatttacatgtaatccagcatcgagtgatagaaaaaatgtttgcgattcgttgaatagatacgcttttccgctatcgcataatttgccaatgttcgcctctgtacatgcagcagaaacacctagacttatgaaagatggttggaaaatttattcagctgaaaaagaatatgaacgccttggaatcccgaattcacgtctttggaaagaagtggacattaataaagactataaatttagcgaaacttatccaagaacttttgtaattccgacagtatcttgggaggaaggaaaaccatttgtaaagaagctcggcgagtttagaagcaaagaaagaattcccgtgttgagttggattaatcagacaactttagcttcaatttcacgatgctcacagccaatgactggaatttctggaaaaagaagtgctgaggacgagcgacatctcacgaacattatgaatgccaatgctaattgtcgtgaacttctgatccttgatgcaagaccagcagtcaatgcaaaacttaataaggcaaaaggtggaggttatgaagagaattatgtcaatgctcctctaacattcctcaatattcacaatattcacgttgttcgggattcattaaaacgattgctagccgcgctgattccacgtgtcgacgagaagggatactacaaagcgttagacgaatcgaaatggctcaatcatgttcagagcattttggaaggagcagtaaaagctgtcttcaatgttgacactgaaaaacaatcagtattgattcattgttctgatggttgggatcgaactgcacagctgacgtcgttggcaatgattcaactcgactcctattaccgtacaattgaaggatttatcgttttgattgagaaggaatggtgtagctttggtcataaatttggagagagaatcggtcacggcgacgataactacagcgatggagaacgatctcccgtatttgttcaattctgtgactgcctatggcaaattatgcgacaatttccatgggcatttgaatttacacaagaactgctaatttgtatgctcgatgagctctacgcttgcagatatggaacatttttgtacaattccgaaaaaatccggctgaaggataagaaatgcgatgaaacgacgatatcgttctggtcctatgttttggaaaataaaaagaaattcagaaatccaatgtttaagcatggaaaatccaataaagtgataaacgtaaacccatctctctgcggtttacatgtatggattgattactacgcccgatcgaatccatatgtagttactccaaatcatgaggatgttcaacagccaggcgcacagtttgtcgacgagaaaaagcaattgttggatgagattatggctcttgacgacgctgctcaaaaattgaccgcctactagagggtagttccctatactagagggtagcgatggttccggtagcggttccggttctatcactattagggctgcttttttggaaaaagaaaatactgctttgagaactgaaattgctgaattggaaaaagaagttggtagatgtgaaaatattgtttctaaatatgaaactagatatggtccattgtactagagtaatcgcggatccgcgaatttcttatgatttatgatttttattattaaataagttataaaaaaaaataagtgtatacaaattttaaagtgactcttaggttttaaaacgaaaattcttattcttgagtaactctttcctgtggtcaggttgctttctcaggtatagcatgaggtcgctcttattgaccacacc BBa_I766215_sequence 1 atgctcgagggagct BBa_I766557_sequence 1 cacgaagtgactgacagaatactgacatcagctgatttctcatagagctgtttctctgataacacgttgtttgaacatcgacaagatgaaaatctagaagtatcaagtttcctttaaagggatatataacagattctaaaactgacagaaatatttcgagtgaagaagaagcgttaaatattggatctttccgcagttctactctgatacatttttgaagtaggagagtcatttagaaggcgtattgctcaatagtagaaagcaggcctgtgcacatgaattaattaaaaaatataaaggtagtgattagacgacacatgtccataggtaacctgtcataattttgaacaatttcccttcttttcttttttttttttgggtgcggcgatatgtagcttgttaatttacacatcatgtacttttctgcatcaaaatatgaaaggcgatagtagctaaagaaaataccgagaatttcctcgaaaagttgacgacaaaagaaaggcataaaaaagtaatttgaaaatattttaaaactgttttaacccatctagcatccgcgctaaaaaaggaagatacaggatacagcggaaacaacttttaa BBa_I766217_sequence 1 taatcgcggatccgcgaatttcttatgatttatgatttttattattaaataagttataaaaaaaaataagtgtatacaaattttaaagtgactcttaggttttaaaacgaaaattcttattcttgagtaactctttcctgtggtcaggttgctttctcaggtatagcatgaggtcgctcttattgaccacacc BBa_I766203_sequence 1 gatgacagagggaacaatagtggagaagttggagaatttgccagttcatcaatgatacaagaatcaatagatctcaaattgttagccgcagaaagtcttatttggacagagaaaaatgtgacatacttcggaccacttggaaaatttccaggtaaaatcgtaattactaggtatcgaatggtttttctcgttggtgatggtggaaaaatgtatgagcaatggaagctcgatattccactcggacaagtgtctcggatagaaaaagttggtagaaagacgacgagtgttgccaaacgaggcgatgataattatggattcacaatttattgtaaagattatcgagtttatcgatttacatgtaatccagcatcgagtgatagaaaaaatgtttgcgattcgttgaatagatacgcttttccgctatcgcataatttgccaatgttcgcctctgtacatgcagcagaaacacctagacttatgaaagatggttggaaaatttattcagctgaaaaagaatatgaacgccttggaatcccgaattcacgtctttggaaagaagtggacattaataaagactataaatttagcgaaacttatccaagaacttttgtaattccgacagtatcttgggaggaaggaaaaccatttgtaaagaagctcggcgagtttagaagcaaagaaagaattcccgtgttgagttggattaatcagacaactttagcttcaatttcacgatgctcacagccaatgactggaatttctggaaaaagaagtgctgaggacgagcgacatctcacgaacattatgaatgccaatgctaattgtcgtgaacttctgatccttgatgcaagaccagcagtcaatgcaaaacttaataaggcaaaaggtggaggttatgaagagaattatgtcaatgctcctctaacattcctcaatattcacaatattcacgttgttcgggattcattaaaacgattgctagccgcgctgattccacgtgtcgacgagaagggatactacaaagcgttagacgaatcgaaatggctcaatcatgttcagagcattttggaaggagcagtaaaagctgtcttcaatgttgacactgaaaaacaatcagtattgattcattgttctgatggttgggatcgaactgcacagctgacgtcgttggcaatgattcaactcgactcctattaccgtacaattgaaggatttatcgttttgattgagaaggaatggtgtagctttggtcataaatttggagagagaatcggtcacggcgacgataactacagcgatggagaacgatctcccgtatttgttcaattctgtgactgcctatggcaaattatgcgacaatttccatgggcatttgaatttacacaagaactgctaatttgtatgctcgatgagctctacgcttgcagatatggaacatttttgtacaattccgaaaaaatccggctgaaggataagaaatgcgatgaaacgacgatatcgttctggtcctatgttttggaaaataaaaagaaattcagaaatccaatgtttaagcatggaaaatccaataaagtgataaacgtaaacccatctctctgcggtttacatgtatggattgattactacgcccgatcgaatccatatgtagttactccaaatcatgaggatgttcaacagccaggcgcacagtttgtcgacgagaaaaagcaattgttggatgagattatggctcttgacgacgctgctcaaaaattgaccgcc igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z