BBa_I766101 1 BBa_I766101 mCherry/RFP 2007-08-08T11:00:00Z 2015-08-31T04:08:09Z Originally isolated from Discosoma striata. A protein that fluoresces red used to tag anything. false false _155_ 0 2018 9 Not in stock false Not a standard Biobrick. Cloned using primers containing adaptor regions for combinatorial cloning using type IIs restriction enzymes. false Robert Ovadia BBa_I766557 1 BBa_I766557 pSte5 (Weak) Promoter 2007-08-15T11:00:00Z 2015-08-31T04:08:10Z endogenous promoter for Sterile 5 low expression, constitutive promoter in yeast false false _155_ 0 1931 9 Not in stock false none false Nili Sommovilla BBa_I766600 1 BBa_I766600 Combinatorial Terminator 2007-08-15T11:00:00Z 2015-08-31T04:08:10Z Synthetic Final adaptors, followed by ???D???, followed by BamHI, 3 STOPS in three different frames, then NotI, the Adh terminator and SacI false false _155_ 0 1931 9 Not in stock false designed specifically for use in ABCD combinatorial cloning methods false Nili Sommovilla BBa_I766002 1 BBa_I766002 YopJ, Irrev. MAPKK Ser/Thr Acetylase 2007-08-08T11:00:00Z 2015-08-31T04:08:09Z Yersinia Pestis YopJ is a MapKK Serine/Threonine Acetylase that serves as a negative effector. Non-reversible reaction. false false _155_ 0 2019 9 Not in stock false Combinatorial cloning with these piece might require primers best suited for your combination. Not in Bio-Brick registrar. Cloned using primers containing adaptor regions for combinatorial cloning using Type IIS enzymes. false Jimmy Jie Min Huang BBa_I766645 1 BBa_I766645 YopJ-EE under weak constitutive promotor 2007-08-15T11:00:00Z 2015-08-31T04:08:10Z composite part This device will express YopJ, a pathogen derived irreversible inhibitor (acetylase) of MapKK Ste7 under the pSte5 promotor. The effector protein is fused to an EE leucine zipper and will be recruited to elements possessing a matching RR zipper. The effector is fused mCherry (RFP) on the N terminal. false false _155_ 0 2016 9 Not in stock false Device was created using a method of combinatorial cloning using the type IIs restriction endonucleases AarI. Each device contains adaptor regions in between coding regions (RFP, effectors, and zippers) for this method. false alexander ng component1941536 1 BBa_I766101 component1941535 1 BBa_I766557 component1941539 1 BBa_I766600 component1941537 1 BBa_I766002 component1941538 1 BBa_I766102 annotation1941537 1 BBa_I766002 range1941537 1 1336 2208 annotation1941538 1 BBa_I766102 range1941538 1 2217 2378 annotation1941539 1 BBa_I766600 range1941539 1 2387 2604 annotation1941536 1 BBa_I766101 range1941536 1 608 1327 annotation1941535 1 BBa_I766557 range1941535 1 1 601 BBa_I766102 1 BBa_I766102 EE Leucine Zipper 2007-08-08T11:00:00Z 2015-08-31T04:08:09Z to be determined binds with the complementary zipper, RR. Can be used to create binding interactions between desired elements through fusing the zipper pair to the appropriate genes. false false _155_ 0 1931 9 Not in stock false not a standard biobrick. cloned using primers containing an adaptor region for combinatorial cloning using type IIs restriction enzymes. false Nili Sommovilla BBa_I766600_sequence 1 taatgcgggatcctgagtaaataagcggccgcgcgaatttcttatgatttatgatttttattattaaataagttataaaaaaaaataagtgtatacaaattttaaagtgactcttaggttttaaaacgaaaattcttattcttgagtaactctttcctgtggtcaggttgctttctcaggtatagcatgaggtcgctcttattgaccacaccgagctc BBa_I766102_sequence 1 ggtagcgatggttccggtagcggttccggttctatcactattagggctgcttttttggaaaaagaaaatactgctttgagaactgaaattgctgaattggaaaaagaagttggtagatgtgaaaatattgtttctaaatatgaaactagatatggtccattg BBa_I766002_sequence 1 ggtagttccctaatcggaccaatatcacaaataaatatctccggtggcttatcagaaaaagagaccagttctttaatcagtaatgaagagcttaaaaatatcataacacagttggaaactgatatatcggatggatcctggttccataaaaattattcacgtatggatgtagaagtcatgcccgcattggtaatccaggcgaacaataaatatccggaaatgaatcttaatcttgttacatctccattggacctttcaatagaaataaaaaacgtcatagaaaatggagttagatcttcccgcttcataattaacatgggggaaggtggaatacatttcagtgtaattgattacaaacatataaatgggaaaacatctctgatattgtttgaaccagcaaactttaacagtatggggccagcgatgctggcaataaggacaaaaacggctattgaacgttatcaattacctgattgccatttctccatggtggaaatggatattcagcgaagctcatctgaatgtggtatttttagtttggcactggcaaaaaaactttacatcgagagagatagcctgttgaaaatacatgaagataatataaaaggtatattaagtgatggtgaaaatcctttaccccacgataagttggacccgtatctcccggtaactttttacaaacatactcaaggtaaaaaacgtcttaatgaatatttaaatactaacccgcagggagttggtactgttgttaacaaaaaaaatgaaaccatcgttaatagatttgataacaataaatccattgtagatggaaaggaattatcagtttcggtacataaaaagagaatagctgaatataaaacacttctcaaagta BBa_I766645_sequence 1 cacgaagtgactgacagaatactgacatcagctgatttctcatagagctgtttctctgataacacgttgtttgaacatcgacaagatgaaaatctagaagtatcaagtttcctttaaagggatatataacagattctaaaactgacagaaatatttcgagtgaagaagaagcgttaaatattggatctttccgcagttctactctgatacatttttgaagtaggagagtcatttagaaggcgtattgctcaatagtagaaagcaggcctgtgcacatgaattaattaaaaaatataaaggtagtgattagacgacacatgtccataggtaacctgtcataattttgaacaatttcccttcttttcttttttttttttgggtgcggcgatatgtagcttgttaatttacacatcatgtacttttctgcatcaaaatatgaaaggcgatagtagctaaagaaaataccgagaatttcctcgaaaagttgacgacaaaagaaaggcataaaaaagtaatttgaaaatattttaaaactgttttaacccatctagcatccgcgctaaaaaaggaagatacaggatacagcggaaacaacttttaatactagatgctcgagggagctgtgagcaagggcgaggaggataacatggccatcatcaaggagttcatgcgcttcaaggtgcacatggagggctccgtgaacggccacgagttcgagatcgagggcgagggcgagggccgcccctacgagggcacccagaccgccaagctgaaggtgaccaagggtggccccctgcccttcgcctgggacatcctgtcccctcagttcatgtacggctccaaggcctacgtgaagcaccccgccgacatccccgactacttgaagctgtccttccccgagggcttcaagtgggagcgcgtgatgaacttcgaggacggcggcgtggtgaccgtgacccaggactcctccctgcaggacggcgagttcatctacaaggtgaagctgcgcggcaccaacttcccctccgacggccccgtaatgcagaagaagaccatgggctgggaggcctcctccgagcggatgtaccccgaggacggcgccctgaagggcgagatcaagcagaggctgaagctgaaggacggcggccactacgacgctgaggtcaagaccacctacaaggccaagaagcccgtgcagctgcccggcgcctacaacgtcaacatcaagttggacatcacctcccacaacgaggactacaccatcgtggaacagtacgaacgcgccgagggccgccactccaccggcggcatggacgagctgtacaagtactagagggtagttccctaatcggaccaatatcacaaataaatatctccggtggcttatcagaaaaagagaccagttctttaatcagtaatgaagagcttaaaaatatcataacacagttggaaactgatatatcggatggatcctggttccataaaaattattcacgtatggatgtagaagtcatgcccgcattggtaatccaggcgaacaataaatatccggaaatgaatcttaatcttgttacatctccattggacctttcaatagaaataaaaaacgtcatagaaaatggagttagatcttcccgcttcataattaacatgggggaaggtggaatacatttcagtgtaattgattacaaacatataaatgggaaaacatctctgatattgtttgaaccagcaaactttaacagtatggggccagcgatgctggcaataaggacaaaaacggctattgaacgttatcaattacctgattgccatttctccatggtggaaatggatattcagcgaagctcatctgaatgtggtatttttagtttggcactggcaaaaaaactttacatcgagagagatagcctgttgaaaatacatgaagataatataaaaggtatattaagtgatggtgaaaatcctttaccccacgataagttggacccgtatctcccggtaactttttacaaacatactcaaggtaaaaaacgtcttaatgaatatttaaatactaacccgcagggagttggtactgttgttaacaaaaaaaatgaaaccatcgttaatagatttgataacaataaatccattgtagatggaaaggaattatcagtttcggtacataaaaagagaatagctgaatataaaacacttctcaaagtatactagagggtagcgatggttccggtagcggttccggttctatcactattagggctgcttttttggaaaaagaaaatactgctttgagaactgaaattgctgaattggaaaaagaagttggtagatgtgaaaatattgtttctaaatatgaaactagatatggtccattgtactagagtaatgcgggatcctgagtaaataagcggccgcgcgaatttcttatgatttatgatttttattattaaataagttataaaaaaaaataagtgtatacaaattttaaagtgactcttaggttttaaaacgaaaattcttattcttgagtaactctttcctgtggtcaggttgctttctcaggtatagcatgaggtcgctcttattgaccacaccgagctc BBa_I766101_sequence 1 atgctcgagggagctgtgagcaagggcgaggaggataacatggccatcatcaaggagttcatgcgcttcaaggtgcacatggagggctccgtgaacggccacgagttcgagatcgagggcgagggcgagggccgcccctacgagggcacccagaccgccaagctgaaggtgaccaagggtggccccctgcccttcgcctgggacatcctgtcccctcagttcatgtacggctccaaggcctacgtgaagcaccccgccgacatccccgactacttgaagctgtccttccccgagggcttcaagtgggagcgcgtgatgaacttcgaggacggcggcgtggtgaccgtgacccaggactcctccctgcaggacggcgagttcatctacaaggtgaagctgcgcggcaccaacttcccctccgacggccccgtaatgcagaagaagaccatgggctgggaggcctcctccgagcggatgtaccccgaggacggcgccctgaagggcgagatcaagcagaggctgaagctgaaggacggcggccactacgacgctgaggtcaagaccacctacaaggccaagaagcccgtgcagctgcccggcgcctacaacgtcaacatcaagttggacatcacctcccacaacgaggactacaccatcgtggaacagtacgaacgcgccgagggccgccactccaccggcggcatggacgagctgtacaag BBa_I766557_sequence 1 cacgaagtgactgacagaatactgacatcagctgatttctcatagagctgtttctctgataacacgttgtttgaacatcgacaagatgaaaatctagaagtatcaagtttcctttaaagggatatataacagattctaaaactgacagaaatatttcgagtgaagaagaagcgttaaatattggatctttccgcagttctactctgatacatttttgaagtaggagagtcatttagaaggcgtattgctcaatagtagaaagcaggcctgtgcacatgaattaattaaaaaatataaaggtagtgattagacgacacatgtccataggtaacctgtcataattttgaacaatttcccttcttttcttttttttttttgggtgcggcgatatgtagcttgttaatttacacatcatgtacttttctgcatcaaaatatgaaaggcgatagtagctaaagaaaataccgagaatttcctcgaaaagttgacgacaaaagaaaggcataaaaaagtaatttgaaaatattttaaaactgttttaacccatctagcatccgcgctaaaaaaggaagatacaggatacagcggaaacaacttttaa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z