BBa_I766555 1 BBa_I766555 pCyc (Medium) Promoter 2007-08-15T11:00:00Z 2015-08-31T04:08:10Z Endogenous promoter for Cytochrome c Mid-expression level constitutive promoter in yeast false false _155_ 0 1931 9 Not in stock false none false Nili Sommovilla BBa_I766101 1 BBa_I766101 mCherry/RFP 2007-08-08T11:00:00Z 2015-08-31T04:08:09Z Originally isolated from Discosoma striata. A protein that fluoresces red used to tag anything. false false _155_ 0 2018 9 Not in stock false Not a standard Biobrick. Cloned using primers containing adaptor regions for combinatorial cloning using type IIs restriction enzymes. false Robert Ovadia BBa_I766675 1 BBa_I766675 YopH-RR under medium constitutive promoter 2007-08-15T11:00:00Z 2015-08-31T04:08:10Z composite part This device will consititutively express YopH, a pathogen derived reversible inhibitor (phosphatase) of Map Kinase Fus3, under the pCyc promoter. The effector protein is fused to a RR zipper, and will be recruited to elements possessing a matching EE zipper. The effector is fused to mCherry (RFP) on the N-terminus. false false _155_ 0 2015 9 It's complicated true Device was created using a method of combinatorial cloning using the type IIs restriction endonuclease AarI. Each device contains adaptor regions in between coding regions (RFP, effectors, and zippers) for this method. false Lauren Jann component1941506 1 BBa_I766101 component1941508 1 BBa_I766103 component1941507 1 BBa_I766007 component1941509 1 BBa_I766600 component1941505 1 BBa_I766555 annotation1941508 1 BBa_I766103 range1941508 1 2400 2561 annotation1941505 1 BBa_I766555 range1941505 1 1 244 annotation1941507 1 BBa_I766007 range1941507 1 979 2391 annotation1941509 1 BBa_I766600 range1941509 1 2570 2787 annotation1941506 1 BBa_I766101 range1941506 1 251 970 BBa_I766600 1 BBa_I766600 Combinatorial Terminator 2007-08-15T11:00:00Z 2015-08-31T04:08:10Z Synthetic Final adaptors, followed by ???D???, followed by BamHI, 3 STOPS in three different frames, then NotI, the Adh terminator and SacI false false _155_ 0 1931 9 Not in stock false designed specifically for use in ABCD combinatorial cloning methods false Nili Sommovilla BBa_I766103 1 BBa_I766103 RR Leucine Zipper 2007-08-08T11:00:00Z 2015-08-31T04:08:09Z tbd. Binds with complementary zipper EE, can be used to create binding interactions between desired elements through zipper paired to appropriate gene. false false _155_ 0 2019 9 Not in stock false Non-BioBrick. Cloned using primers containing adaptor regions for combinatorial cloning using Type IIS restriction enzymes. false Jimmy Jie Min Huang BBa_I766007 1 BBa_I766007 YopH, Rev. MAPK Tyr Phosphatase 2007-08-08T11:00:00Z 2015-08-31T04:08:09Z Yersinia Pestis Reversible Repressor of MAPK Fus3 in yeast mating pathway by removing a phosphate group. false false _155_ 0 2017 9 Not in stock false Not a standard BioBrick. Cloned using primers containing adaptor regions for combinatorial cloning using type IIs restriction enzyme. false Eric Chou BBa_I766600_sequence 1 taatgcgggatcctgagtaaataagcggccgcgcgaatttcttatgatttatgatttttattattaaataagttataaaaaaaaataagtgtatacaaattttaaagtgactcttaggttttaaaacgaaaattcttattcttgagtaactctttcctgtggtcaggttgctttctcaggtatagcatgaggtcgctcttattgaccacaccgagctc BBa_I766555_sequence 1 cagatccgccaggcgtgtatatatagcgtggatggccaggcaactttagtgctgacacatacaggcatatatatatgtgtgcgacgacacatgatcatatggcatgcatgtgctctgtatgtatataaaactcttgttttcttcttttctctaaatattctttccttatacattaggacctttgcagcataaattactatacttctatagacacacaaacacaaatacacacactaaattaata BBa_I766007_sequence 1 ggtagttccctaaacttatcattaagcgatcttcatcgtcaggtatctcgattggtgcagcaagagagcggtgattgtaccgggaaattaagaggtaacgttgctgccaataaagaaactacctttcaaggtttgaccatagccagtggagccagagagtcagaaaaagtatttgctcaaactgtactaagccacgtagcaaatgttgttctaactcaagaagataccgctaagctattgcaaagtacggtaaagcataatttgaataattatgacttaagaagtgtcggcaatggtaatagtgtacttgtcagtttacgtagtgaccaaatgacactacaagacgccaaagtgctgttggaggccgcattgcgacaagagtcgggagcgagggggcatgtatcatctcattcacattcagcccttcacgcaccgggaaccccggtgcgtgaaggactgcgttcacatctagaccccagaactccaccgttgccaccgcgtgaacgaccacacacttctggccatcacggggctggcgaagccagagccaccgcaccaagcactgtttctccttatggcccagaagcgcgcgcagaactcagcagccgcctcaccacattgcgcaatacgctggcgccagcaacgaatgatccgcgttacttacaagcctgcggcggtgaaaagctaaaccgatttagagatattcaatgctgtcggcaaaccgcagtacgcgccgatcttaatgccaattacatccaggtcggtaacactcgtaccatagcgtgccagtatccgctacaatctcaacttgaaagccatttccgtatgctggcagaaaaccgaacgccagtgttggctgttttagcgtccagttctgagatagccaatcaaagattcggtatgccagattatttccgccagagtggtacctatggcagtatcactgtagagtctaaaatgactcagcaagttggtctcggtgacgggattatggcagatatgtatactttaacgattcgtgaagcgggtcaaaaaacaatctctgttcctgtggttcatgttggcaattggcccgatcagaccgcagtcagctctgaagttaccaaggcactcgcttcactggtagatcaaacagcagaaacaaaacgcaatatgtatgaaagcaaaggaagttcagcggtaggagatgactccaaattacggccggtaatacattgccgtgcgggtgttggccgtactgcgcaactgattggcgcaatgtgcatgaatgatagtcgtaatagtcagttaagcgtagaagatatggtcagccaaatgcgagtacaaagaaatggtattatggtacaaaaagatgagcaacttgatgttctgattaagttggctgaaggacaagggcgaccattattaaatagc BBa_I766101_sequence 1 atgctcgagggagctgtgagcaagggcgaggaggataacatggccatcatcaaggagttcatgcgcttcaaggtgcacatggagggctccgtgaacggccacgagttcgagatcgagggcgagggcgagggccgcccctacgagggcacccagaccgccaagctgaaggtgaccaagggtggccccctgcccttcgcctgggacatcctgtcccctcagttcatgtacggctccaaggcctacgtgaagcaccccgccgacatccccgactacttgaagctgtccttccccgagggcttcaagtgggagcgcgtgatgaacttcgaggacggcggcgtggtgaccgtgacccaggactcctccctgcaggacggcgagttcatctacaaggtgaagctgcgcggcaccaacttcccctccgacggccccgtaatgcagaagaagaccatgggctgggaggcctcctccgagcggatgtaccccgaggacggcgccctgaagggcgagatcaagcagaggctgaagctgaaggacggcggccactacgacgctgaggtcaagaccacctacaaggccaagaagcccgtgcagctgcccggcgcctacaacgtcaacatcaagttggacatcacctcccacaacgaggactacaccatcgtggaacagtacgaacgcgccgagggccgccactccaccggcggcatggacgagctgtacaag BBa_I766675_sequence 1 cagatccgccaggcgtgtatatatagcgtggatggccaggcaactttagtgctgacacatacaggcatatatatatgtgtgcgacgacacatgatcatatggcatgcatgtgctctgtatgtatataaaactcttgttttcttcttttctctaaatattctttccttatacattaggacctttgcagcataaattactatacttctatagacacacaaacacaaatacacacactaaattaatatactagatgctcgagggagctgtgagcaagggcgaggaggataacatggccatcatcaaggagttcatgcgcttcaaggtgcacatggagggctccgtgaacggccacgagttcgagatcgagggcgagggcgagggccgcccctacgagggcacccagaccgccaagctgaaggtgaccaagggtggccccctgcccttcgcctgggacatcctgtcccctcagttcatgtacggctccaaggcctacgtgaagcaccccgccgacatccccgactacttgaagctgtccttccccgagggcttcaagtgggagcgcgtgatgaacttcgaggacggcggcgtggtgaccgtgacccaggactcctccctgcaggacggcgagttcatctacaaggtgaagctgcgcggcaccaacttcccctccgacggccccgtaatgcagaagaagaccatgggctgggaggcctcctccgagcggatgtaccccgaggacggcgccctgaagggcgagatcaagcagaggctgaagctgaaggacggcggccactacgacgctgaggtcaagaccacctacaaggccaagaagcccgtgcagctgcccggcgcctacaacgtcaacatcaagttggacatcacctcccacaacgaggactacaccatcgtggaacagtacgaacgcgccgagggccgccactccaccggcggcatggacgagctgtacaagtactagagggtagttccctaaacttatcattaagcgatcttcatcgtcaggtatctcgattggtgcagcaagagagcggtgattgtaccgggaaattaagaggtaacgttgctgccaataaagaaactacctttcaaggtttgaccatagccagtggagccagagagtcagaaaaagtatttgctcaaactgtactaagccacgtagcaaatgttgttctaactcaagaagataccgctaagctattgcaaagtacggtaaagcataatttgaataattatgacttaagaagtgtcggcaatggtaatagtgtacttgtcagtttacgtagtgaccaaatgacactacaagacgccaaagtgctgttggaggccgcattgcgacaagagtcgggagcgagggggcatgtatcatctcattcacattcagcccttcacgcaccgggaaccccggtgcgtgaaggactgcgttcacatctagaccccagaactccaccgttgccaccgcgtgaacgaccacacacttctggccatcacggggctggcgaagccagagccaccgcaccaagcactgtttctccttatggcccagaagcgcgcgcagaactcagcagccgcctcaccacattgcgcaatacgctggcgccagcaacgaatgatccgcgttacttacaagcctgcggcggtgaaaagctaaaccgatttagagatattcaatgctgtcggcaaaccgcagtacgcgccgatcttaatgccaattacatccaggtcggtaacactcgtaccatagcgtgccagtatccgctacaatctcaacttgaaagccatttccgtatgctggcagaaaaccgaacgccagtgttggctgttttagcgtccagttctgagatagccaatcaaagattcggtatgccagattatttccgccagagtggtacctatggcagtatcactgtagagtctaaaatgactcagcaagttggtctcggtgacgggattatggcagatatgtatactttaacgattcgtgaagcgggtcaaaaaacaatctctgttcctgtggttcatgttggcaattggcccgatcagaccgcagtcagctctgaagttaccaaggcactcgcttcactggtagatcaaacagcagaaacaaaacgcaatatgtatgaaagcaaaggaagttcagcggtaggagatgactccaaattacggccggtaatacattgccgtgcgggtgttggccgtactgcgcaactgattggcgcaatgtgcatgaatgatagtcgtaatagtcagttaagcgtagaagatatggtcagccaaatgcgagtacaaagaaatggtattatggtacaaaaagatgagcaacttgatgttctgattaagttggctgaaggacaagggcgaccattattaaatagctactagagggtagcgatggttctggttctggttctggttctctggaaatccgtgcggcgttcctggaaaaagaaaacaccgcgctgcgtacccgtgcggcggaactgcgtaaacgtgttggtcgttgccgtaacatcgtttctaaatacgaaacccgttacggtccgctgtactagagtaatgcgggatcctgagtaaataagcggccgcgcgaatttcttatgatttatgatttttattattaaataagttataaaaaaaaataagtgtatacaaattttaaagtgactcttaggttttaaaacgaaaattcttattcttgagtaactctttcctgtggtcaggttgctttctcaggtatagcatgaggtcgctcttattgaccacaccgagctc BBa_I766103_sequence 1 ggtagcgatggttctggttctggttctggttctctggaaatccgtgcggcgttcctggaaaaagaaaacaccgcgctgcgtacccgtgcggcggaactgcgtaaacgtgttggtcgttgccgtaacatcgtttctaaatacgaaacccgttacggtccgctg igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z