BBa_I766685 1 BBa_I766685 YopJ-EE under inducible promotor 2007-08-15T11:00:00Z 2015-08-31T04:08:10Z composite part This device will express YopJ, a pathogen derived irreversible inhibitor (acetylase) of MapKK Ste7 under the pFig promotor. The effector protein is fused to an EE leucine zipper and will be recruited to elements possessing a matching RR zipper. The effector is fused mCherry (RFP) on the N terminal. false false _155_ 0 2016 9 It's complicated true Device was created using a method of combinatorial cloning using the type IIs restriction endonucleases AarI. Each device contains adaptor regions in between coding regions (RFP, effectors, and zippers) for this method. false alexander ng component1941566 1 BBa_I766101 component1941569 1 BBa_I766600 component1941567 1 BBa_I766002 component1941565 1 BBa_I766558 component1941568 1 BBa_I766102 annotation1941567 1 BBa_I766002 range1941567 1 1735 2607 annotation1941568 1 BBa_I766102 range1941568 1 2616 2777 annotation1941569 1 BBa_I766600 range1941569 1 2786 3003 annotation1941566 1 BBa_I766101 range1941566 1 1007 1726 annotation1941565 1 BBa_I766558 range1941565 1 1 1000 BBa_I766558 1 BBa_I766558 pFig1 (Inducible) Promoter 2007-08-15T11:00:00Z 2015-08-31T04:08:10Z endogenous promoter for Fig1 (Factor Induced Gene 1) Inducible promoter in yeast false false _155_ 0 1931 9 Not in stock false none false Nili Sommovilla BBa_I766101 1 BBa_I766101 mCherry/RFP 2007-08-08T11:00:00Z 2015-08-31T04:08:09Z Originally isolated from Discosoma striata. A protein that fluoresces red used to tag anything. false false _155_ 0 2018 9 Not in stock false Not a standard Biobrick. Cloned using primers containing adaptor regions for combinatorial cloning using type IIs restriction enzymes. false Robert Ovadia BBa_I766600 1 BBa_I766600 Combinatorial Terminator 2007-08-15T11:00:00Z 2015-08-31T04:08:10Z Synthetic Final adaptors, followed by ???D???, followed by BamHI, 3 STOPS in three different frames, then NotI, the Adh terminator and SacI false false _155_ 0 1931 9 Not in stock false designed specifically for use in ABCD combinatorial cloning methods false Nili Sommovilla BBa_I766002 1 BBa_I766002 YopJ, Irrev. MAPKK Ser/Thr Acetylase 2007-08-08T11:00:00Z 2015-08-31T04:08:09Z Yersinia Pestis YopJ is a MapKK Serine/Threonine Acetylase that serves as a negative effector. Non-reversible reaction. false false _155_ 0 2019 9 Not in stock false Combinatorial cloning with these piece might require primers best suited for your combination. Not in Bio-Brick registrar. Cloned using primers containing adaptor regions for combinatorial cloning using Type IIS enzymes. false Jimmy Jie Min Huang BBa_I766102 1 BBa_I766102 EE Leucine Zipper 2007-08-08T11:00:00Z 2015-08-31T04:08:09Z to be determined binds with the complementary zipper, RR. Can be used to create binding interactions between desired elements through fusing the zipper pair to the appropriate genes. false false _155_ 0 1931 9 Not in stock false not a standard biobrick. cloned using primers containing an adaptor region for combinatorial cloning using type IIs restriction enzymes. false Nili Sommovilla BBa_I766600_sequence 1 taatgcgggatcctgagtaaataagcggccgcgcgaatttcttatgatttatgatttttattattaaataagttataaaaaaaaataagtgtatacaaattttaaagtgactcttaggttttaaaacgaaaattcttattcttgagtaactctttcctgtggtcaggttgctttctcaggtatagcatgaggtcgctcttattgaccacaccgagctc BBa_I766102_sequence 1 ggtagcgatggttccggtagcggttccggttctatcactattagggctgcttttttggaaaaagaaaatactgctttgagaactgaaattgctgaattggaaaaagaagttggtagatgtgaaaatattgtttctaaatatgaaactagatatggtccattg BBa_I766002_sequence 1 ggtagttccctaatcggaccaatatcacaaataaatatctccggtggcttatcagaaaaagagaccagttctttaatcagtaatgaagagcttaaaaatatcataacacagttggaaactgatatatcggatggatcctggttccataaaaattattcacgtatggatgtagaagtcatgcccgcattggtaatccaggcgaacaataaatatccggaaatgaatcttaatcttgttacatctccattggacctttcaatagaaataaaaaacgtcatagaaaatggagttagatcttcccgcttcataattaacatgggggaaggtggaatacatttcagtgtaattgattacaaacatataaatgggaaaacatctctgatattgtttgaaccagcaaactttaacagtatggggccagcgatgctggcaataaggacaaaaacggctattgaacgttatcaattacctgattgccatttctccatggtggaaatggatattcagcgaagctcatctgaatgtggtatttttagtttggcactggcaaaaaaactttacatcgagagagatagcctgttgaaaatacatgaagataatataaaaggtatattaagtgatggtgaaaatcctttaccccacgataagttggacccgtatctcccggtaactttttacaaacatactcaaggtaaaaaacgtcttaatgaatatttaaatactaacccgcagggagttggtactgttgttaacaaaaaaaatgaaaccatcgttaatagatttgataacaataaatccattgtagatggaaaggaattatcagtttcggtacataaaaagagaatagctgaatataaaacacttctcaaagta BBa_I766558_sequence 1 ttgaatgatcaaccaaacgccgatatagtcactattggtgataaaattaaaatgcagctattgagaacccatcctaacaacattaaattgtctattaatggaattggtaaagatgccccaactttccaagaatctgctttgattgccgataagttattgagtgtcatgaaggccggcacttacccaaagatttccattttctacaatgacccagtgtcttccctatcttttgaaccatctgaaaaaccgatctttaacgccaagaccattgaacaatccccatcattcggcaaatttgagatcgacacggacgcaaacgttccaagagatttgtttgaatatactttggctaaccaaatgttgacagcaatggctcaaggttatgctgctgaaatttccgccagaagaaacgctatggataacgcttccaagaatgccggtgatatgatcaatcgttactctatcttgtacaacagaacaagacaagctgtcattactaatgaactggttgatattattactggtgcttcctctttgggatgataaaaaaatcaccctgcattgcctctttatttgacgttgtttttgtagaacatgaaacgaattttgacttgatgagacgaagtatatatccaaagaataccttaaatagaaaaggaaagataataaatactaaacactactatatattcaggtaaaatacaaaaattataacatttttaaaactttttttttttgaaagtccttctcgctttaggattttttcccattaagattatgatggtttcatgtatgtgtcagttaaaaaaaatatggctaagtagcaatgaaacgaaccaagaaaatgaaaacaatatatagtgctgttgaaataacaaagacattggtatatatttgtaaaatgtctgttaaatgttttttatctcaggttcttgcttgtctttggtagaagaaattatagtaaacaaacaaacaaacaaacaaaaaaaaaaaaaaaaaa BBa_I766101_sequence 1 atgctcgagggagctgtgagcaagggcgaggaggataacatggccatcatcaaggagttcatgcgcttcaaggtgcacatggagggctccgtgaacggccacgagttcgagatcgagggcgagggcgagggccgcccctacgagggcacccagaccgccaagctgaaggtgaccaagggtggccccctgcccttcgcctgggacatcctgtcccctcagttcatgtacggctccaaggcctacgtgaagcaccccgccgacatccccgactacttgaagctgtccttccccgagggcttcaagtgggagcgcgtgatgaacttcgaggacggcggcgtggtgaccgtgacccaggactcctccctgcaggacggcgagttcatctacaaggtgaagctgcgcggcaccaacttcccctccgacggccccgtaatgcagaagaagaccatgggctgggaggcctcctccgagcggatgtaccccgaggacggcgccctgaagggcgagatcaagcagaggctgaagctgaaggacggcggccactacgacgctgaggtcaagaccacctacaaggccaagaagcccgtgcagctgcccggcgcctacaacgtcaacatcaagttggacatcacctcccacaacgaggactacaccatcgtggaacagtacgaacgcgccgagggccgccactccaccggcggcatggacgagctgtacaag BBa_I766685_sequence 1 ttgaatgatcaaccaaacgccgatatagtcactattggtgataaaattaaaatgcagctattgagaacccatcctaacaacattaaattgtctattaatggaattggtaaagatgccccaactttccaagaatctgctttgattgccgataagttattgagtgtcatgaaggccggcacttacccaaagatttccattttctacaatgacccagtgtcttccctatcttttgaaccatctgaaaaaccgatctttaacgccaagaccattgaacaatccccatcattcggcaaatttgagatcgacacggacgcaaacgttccaagagatttgtttgaatatactttggctaaccaaatgttgacagcaatggctcaaggttatgctgctgaaatttccgccagaagaaacgctatggataacgcttccaagaatgccggtgatatgatcaatcgttactctatcttgtacaacagaacaagacaagctgtcattactaatgaactggttgatattattactggtgcttcctctttgggatgataaaaaaatcaccctgcattgcctctttatttgacgttgtttttgtagaacatgaaacgaattttgacttgatgagacgaagtatatatccaaagaataccttaaatagaaaaggaaagataataaatactaaacactactatatattcaggtaaaatacaaaaattataacatttttaaaactttttttttttgaaagtccttctcgctttaggattttttcccattaagattatgatggtttcatgtatgtgtcagttaaaaaaaatatggctaagtagcaatgaaacgaaccaagaaaatgaaaacaatatatagtgctgttgaaataacaaagacattggtatatatttgtaaaatgtctgttaaatgttttttatctcaggttcttgcttgtctttggtagaagaaattatagtaaacaaacaaacaaacaaacaaaaaaaaaaaaaaaaaatactagatgctcgagggagctgtgagcaagggcgaggaggataacatggccatcatcaaggagttcatgcgcttcaaggtgcacatggagggctccgtgaacggccacgagttcgagatcgagggcgagggcgagggccgcccctacgagggcacccagaccgccaagctgaaggtgaccaagggtggccccctgcccttcgcctgggacatcctgtcccctcagttcatgtacggctccaaggcctacgtgaagcaccccgccgacatccccgactacttgaagctgtccttccccgagggcttcaagtgggagcgcgtgatgaacttcgaggacggcggcgtggtgaccgtgacccaggactcctccctgcaggacggcgagttcatctacaaggtgaagctgcgcggcaccaacttcccctccgacggccccgtaatgcagaagaagaccatgggctgggaggcctcctccgagcggatgtaccccgaggacggcgccctgaagggcgagatcaagcagaggctgaagctgaaggacggcggccactacgacgctgaggtcaagaccacctacaaggccaagaagcccgtgcagctgcccggcgcctacaacgtcaacatcaagttggacatcacctcccacaacgaggactacaccatcgtggaacagtacgaacgcgccgagggccgccactccaccggcggcatggacgagctgtacaagtactagagggtagttccctaatcggaccaatatcacaaataaatatctccggtggcttatcagaaaaagagaccagttctttaatcagtaatgaagagcttaaaaatatcataacacagttggaaactgatatatcggatggatcctggttccataaaaattattcacgtatggatgtagaagtcatgcccgcattggtaatccaggcgaacaataaatatccggaaatgaatcttaatcttgttacatctccattggacctttcaatagaaataaaaaacgtcatagaaaatggagttagatcttcccgcttcataattaacatgggggaaggtggaatacatttcagtgtaattgattacaaacatataaatgggaaaacatctctgatattgtttgaaccagcaaactttaacagtatggggccagcgatgctggcaataaggacaaaaacggctattgaacgttatcaattacctgattgccatttctccatggtggaaatggatattcagcgaagctcatctgaatgtggtatttttagtttggcactggcaaaaaaactttacatcgagagagatagcctgttgaaaatacatgaagataatataaaaggtatattaagtgatggtgaaaatcctttaccccacgataagttggacccgtatctcccggtaactttttacaaacatactcaaggtaaaaaacgtcttaatgaatatttaaatactaacccgcagggagttggtactgttgttaacaaaaaaaatgaaaccatcgttaatagatttgataacaataaatccattgtagatggaaaggaattatcagtttcggtacataaaaagagaatagctgaatataaaacacttctcaaagtatactagagggtagcgatggttccggtagcggttccggttctatcactattagggctgcttttttggaaaaagaaaatactgctttgagaactgaaattgctgaattggaaaaagaagttggtagatgtgaaaatattgtttctaaatatgaaactagatatggtccattgtactagagtaatgcgggatcctgagtaaataagcggccgcgcgaatttcttatgatttatgatttttattattaaataagttataaaaaaaaataagtgtatacaaattttaaagtgactcttaggttttaaaacgaaaattcttattcttgagtaactctttcctgtggtcaggttgctttctcaggtatagcatgaggtcgctcttattgaccacaccgagctc igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z