BBa_I766998
1
BBa_I766998
YopJ-RR under inducible promotor
2007-08-15T11:00:00Z
2015-08-31T04:08:10Z
Composite Part.
This device will express YopJ, a pathogen derived irreversible inhibitor (acetylase) of MapKK Ste7 under the pFig1 promotor. The effector protein is fused to an RR leucine zipper and will be recruited to elements possessing the matching EE zipper. The effector is fused mCherry (RFP) on the N-terminal.
false
false
_155_
0
2019
9
It's complicated
true
Device was created using a method of combinatorial cloning using the type IIs restriction endonuclease AarI. Each device contains adaptor regions in between coding regions (RFP, effectors, and zippers) for this method.
false
Jimmy Jie Min Huang
component1941579
1
BBa_I766600
component1941576
1
BBa_I766002
component1941578
1
BBa_I766103
component1941575
1
BBa_I766558
component1941577
1
BBa_I766101
annotation1941575
1
BBa_I766558
range1941575
1
1
1000
annotation1941576
1
BBa_I766002
range1941576
1
1009
1881
annotation1941579
1
BBa_I766600
range1941579
1
2786
3003
annotation1941577
1
BBa_I766101
range1941577
1
1888
2607
annotation1941578
1
BBa_I766103
range1941578
1
2616
2777
BBa_I766558
1
BBa_I766558
pFig1 (Inducible) Promoter
2007-08-15T11:00:00Z
2015-08-31T04:08:10Z
endogenous promoter for Fig1 (Factor Induced Gene 1)
Inducible promoter in yeast
false
false
_155_
0
1931
9
Not in stock
false
none
false
Nili Sommovilla
BBa_I766101
1
BBa_I766101
mCherry/RFP
2007-08-08T11:00:00Z
2015-08-31T04:08:09Z
Originally isolated from Discosoma striata.
A protein that fluoresces red used to tag anything.
false
false
_155_
0
2018
9
Not in stock
false
Not a standard Biobrick. Cloned using primers containing adaptor regions for combinatorial cloning using type IIs restriction enzymes.
false
Robert Ovadia
BBa_I766600
1
BBa_I766600
Combinatorial Terminator
2007-08-15T11:00:00Z
2015-08-31T04:08:10Z
Synthetic
Final adaptors, followed by ???D???, followed by BamHI, 3 STOPS in three different frames, then NotI, the Adh terminator and SacI
false
false
_155_
0
1931
9
Not in stock
false
designed specifically for use in ABCD combinatorial cloning methods
false
Nili Sommovilla
BBa_I766002
1
BBa_I766002
YopJ, Irrev. MAPKK Ser/Thr Acetylase
2007-08-08T11:00:00Z
2015-08-31T04:08:09Z
Yersinia Pestis
YopJ is a MapKK Serine/Threonine Acetylase that serves as a negative effector. Non-reversible reaction.
false
false
_155_
0
2019
9
Not in stock
false
Combinatorial cloning with these piece might require primers best suited for your combination. Not in Bio-Brick registrar. Cloned using primers containing adaptor regions for combinatorial cloning using Type IIS enzymes.
false
Jimmy Jie Min Huang
BBa_I766103
1
BBa_I766103
RR Leucine Zipper
2007-08-08T11:00:00Z
2015-08-31T04:08:09Z
tbd.
Binds with complementary zipper EE, can be used to create binding interactions between desired elements through zipper paired to appropriate gene.
false
false
_155_
0
2019
9
Not in stock
false
Non-BioBrick. Cloned using primers containing adaptor regions for combinatorial cloning using Type IIS restriction enzymes.
false
Jimmy Jie Min Huang
BBa_I766600_sequence
1
taatgcgggatcctgagtaaataagcggccgcgcgaatttcttatgatttatgatttttattattaaataagttataaaaaaaaataagtgtatacaaattttaaagtgactcttaggttttaaaacgaaaattcttattcttgagtaactctttcctgtggtcaggttgctttctcaggtatagcatgaggtcgctcttattgaccacaccgagctc
BBa_I766002_sequence
1
ggtagttccctaatcggaccaatatcacaaataaatatctccggtggcttatcagaaaaagagaccagttctttaatcagtaatgaagagcttaaaaatatcataacacagttggaaactgatatatcggatggatcctggttccataaaaattattcacgtatggatgtagaagtcatgcccgcattggtaatccaggcgaacaataaatatccggaaatgaatcttaatcttgttacatctccattggacctttcaatagaaataaaaaacgtcatagaaaatggagttagatcttcccgcttcataattaacatgggggaaggtggaatacatttcagtgtaattgattacaaacatataaatgggaaaacatctctgatattgtttgaaccagcaaactttaacagtatggggccagcgatgctggcaataaggacaaaaacggctattgaacgttatcaattacctgattgccatttctccatggtggaaatggatattcagcgaagctcatctgaatgtggtatttttagtttggcactggcaaaaaaactttacatcgagagagatagcctgttgaaaatacatgaagataatataaaaggtatattaagtgatggtgaaaatcctttaccccacgataagttggacccgtatctcccggtaactttttacaaacatactcaaggtaaaaaacgtcttaatgaatatttaaatactaacccgcagggagttggtactgttgttaacaaaaaaaatgaaaccatcgttaatagatttgataacaataaatccattgtagatggaaaggaattatcagtttcggtacataaaaagagaatagctgaatataaaacacttctcaaagta
BBa_I766558_sequence
1
ttgaatgatcaaccaaacgccgatatagtcactattggtgataaaattaaaatgcagctattgagaacccatcctaacaacattaaattgtctattaatggaattggtaaagatgccccaactttccaagaatctgctttgattgccgataagttattgagtgtcatgaaggccggcacttacccaaagatttccattttctacaatgacccagtgtcttccctatcttttgaaccatctgaaaaaccgatctttaacgccaagaccattgaacaatccccatcattcggcaaatttgagatcgacacggacgcaaacgttccaagagatttgtttgaatatactttggctaaccaaatgttgacagcaatggctcaaggttatgctgctgaaatttccgccagaagaaacgctatggataacgcttccaagaatgccggtgatatgatcaatcgttactctatcttgtacaacagaacaagacaagctgtcattactaatgaactggttgatattattactggtgcttcctctttgggatgataaaaaaatcaccctgcattgcctctttatttgacgttgtttttgtagaacatgaaacgaattttgacttgatgagacgaagtatatatccaaagaataccttaaatagaaaaggaaagataataaatactaaacactactatatattcaggtaaaatacaaaaattataacatttttaaaactttttttttttgaaagtccttctcgctttaggattttttcccattaagattatgatggtttcatgtatgtgtcagttaaaaaaaatatggctaagtagcaatgaaacgaaccaagaaaatgaaaacaatatatagtgctgttgaaataacaaagacattggtatatatttgtaaaatgtctgttaaatgttttttatctcaggttcttgcttgtctttggtagaagaaattatagtaaacaaacaaacaaacaaacaaaaaaaaaaaaaaaaaa
BBa_I766101_sequence
1
atgctcgagggagctgtgagcaagggcgaggaggataacatggccatcatcaaggagttcatgcgcttcaaggtgcacatggagggctccgtgaacggccacgagttcgagatcgagggcgagggcgagggccgcccctacgagggcacccagaccgccaagctgaaggtgaccaagggtggccccctgcccttcgcctgggacatcctgtcccctcagttcatgtacggctccaaggcctacgtgaagcaccccgccgacatccccgactacttgaagctgtccttccccgagggcttcaagtgggagcgcgtgatgaacttcgaggacggcggcgtggtgaccgtgacccaggactcctccctgcaggacggcgagttcatctacaaggtgaagctgcgcggcaccaacttcccctccgacggccccgtaatgcagaagaagaccatgggctgggaggcctcctccgagcggatgtaccccgaggacggcgccctgaagggcgagatcaagcagaggctgaagctgaaggacggcggccactacgacgctgaggtcaagaccacctacaaggccaagaagcccgtgcagctgcccggcgcctacaacgtcaacatcaagttggacatcacctcccacaacgaggactacaccatcgtggaacagtacgaacgcgccgagggccgccactccaccggcggcatggacgagctgtacaag
BBa_I766998_sequence
1
ttgaatgatcaaccaaacgccgatatagtcactattggtgataaaattaaaatgcagctattgagaacccatcctaacaacattaaattgtctattaatggaattggtaaagatgccccaactttccaagaatctgctttgattgccgataagttattgagtgtcatgaaggccggcacttacccaaagatttccattttctacaatgacccagtgtcttccctatcttttgaaccatctgaaaaaccgatctttaacgccaagaccattgaacaatccccatcattcggcaaatttgagatcgacacggacgcaaacgttccaagagatttgtttgaatatactttggctaaccaaatgttgacagcaatggctcaaggttatgctgctgaaatttccgccagaagaaacgctatggataacgcttccaagaatgccggtgatatgatcaatcgttactctatcttgtacaacagaacaagacaagctgtcattactaatgaactggttgatattattactggtgcttcctctttgggatgataaaaaaatcaccctgcattgcctctttatttgacgttgtttttgtagaacatgaaacgaattttgacttgatgagacgaagtatatatccaaagaataccttaaatagaaaaggaaagataataaatactaaacactactatatattcaggtaaaatacaaaaattataacatttttaaaactttttttttttgaaagtccttctcgctttaggattttttcccattaagattatgatggtttcatgtatgtgtcagttaaaaaaaatatggctaagtagcaatgaaacgaaccaagaaaatgaaaacaatatatagtgctgttgaaataacaaagacattggtatatatttgtaaaatgtctgttaaatgttttttatctcaggttcttgcttgtctttggtagaagaaattatagtaaacaaacaaacaaacaaacaaaaaaaaaaaaaaaaaatactagagggtagttccctaatcggaccaatatcacaaataaatatctccggtggcttatcagaaaaagagaccagttctttaatcagtaatgaagagcttaaaaatatcataacacagttggaaactgatatatcggatggatcctggttccataaaaattattcacgtatggatgtagaagtcatgcccgcattggtaatccaggcgaacaataaatatccggaaatgaatcttaatcttgttacatctccattggacctttcaatagaaataaaaaacgtcatagaaaatggagttagatcttcccgcttcataattaacatgggggaaggtggaatacatttcagtgtaattgattacaaacatataaatgggaaaacatctctgatattgtttgaaccagcaaactttaacagtatggggccagcgatgctggcaataaggacaaaaacggctattgaacgttatcaattacctgattgccatttctccatggtggaaatggatattcagcgaagctcatctgaatgtggtatttttagtttggcactggcaaaaaaactttacatcgagagagatagcctgttgaaaatacatgaagataatataaaaggtatattaagtgatggtgaaaatcctttaccccacgataagttggacccgtatctcccggtaactttttacaaacatactcaaggtaaaaaacgtcttaatgaatatttaaatactaacccgcagggagttggtactgttgttaacaaaaaaaatgaaaccatcgttaatagatttgataacaataaatccattgtagatggaaaggaattatcagtttcggtacataaaaagagaatagctgaatataaaacacttctcaaagtatactagatgctcgagggagctgtgagcaagggcgaggaggataacatggccatcatcaaggagttcatgcgcttcaaggtgcacatggagggctccgtgaacggccacgagttcgagatcgagggcgagggcgagggccgcccctacgagggcacccagaccgccaagctgaaggtgaccaagggtggccccctgcccttcgcctgggacatcctgtcccctcagttcatgtacggctccaaggcctacgtgaagcaccccgccgacatccccgactacttgaagctgtccttccccgagggcttcaagtgggagcgcgtgatgaacttcgaggacggcggcgtggtgaccgtgacccaggactcctccctgcaggacggcgagttcatctacaaggtgaagctgcgcggcaccaacttcccctccgacggccccgtaatgcagaagaagaccatgggctgggaggcctcctccgagcggatgtaccccgaggacggcgccctgaagggcgagatcaagcagaggctgaagctgaaggacggcggccactacgacgctgaggtcaagaccacctacaaggccaagaagcccgtgcagctgcccggcgcctacaacgtcaacatcaagttggacatcacctcccacaacgaggactacaccatcgtggaacagtacgaacgcgccgagggccgccactccaccggcggcatggacgagctgtacaagtactagagggtagcgatggttctggttctggttctggttctctggaaatccgtgcggcgttcctggaaaaagaaaacaccgcgctgcgtacccgtgcggcggaactgcgtaaacgtgttggtcgttgccgtaacatcgtttctaaatacgaaacccgttacggtccgctgtactagagtaatgcgggatcctgagtaaataagcggccgcgcgaatttcttatgatttatgatttttattattaaataagttataaaaaaaaataagtgtatacaaattttaaagtgactcttaggttttaaaacgaaaattcttattcttgagtaactctttcctgtggtcaggttgctttctcaggtatagcatgaggtcgctcttattgaccacaccgagctc
BBa_I766103_sequence
1
ggtagcgatggttctggttctggttctggttctctggaaatccgtgcggcgttcctggaaaaagaaaacaccgcgctgcgtacccgtgcggcggaactgcgtaaacgtgttggtcgttgccgtaacatcgtttctaaatacgaaacccgttacggtccgctg
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z