LOCUS       BBa_I769103             1197 bp    DNA     linear       08-May-2024
DEFINITION  Feruloyl-CoA hydratase for vanillin biosynthesis protein generator
ACCESSION   BBa_I769103
VERSION     BBa_I769103.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    213..216
                     /label=conserved
     RBS             209..220
                     /label=BBa_B0034
     misc_feature    227..229
                     /label=Start codon
     misc_feature    1055..1060
                     /label=Stop codon
     CDS             227..1060
                     /label=Feruloyl-CoA hydratase (ech)
     CDS             227..1060
                     /label=BBa_I769102
     stem_loop       1080..1123
                     /label=stem_loop
     misc_feature    1069..1148
                     /label=BBa_B0010
     terminator      1069..1148
                     /label=BBa_B0010
     stem_loop       1164..1183
                     /label=T7 TE
     misc_feature    1190..1190
                     /label=stop
     polyA_site      1184..1197
                     /label=polya
     misc_feature    1157..1197
                     /label=BBa_B0012
     terminator      1157..1197
                     /label=BBa_B0012
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatga gcaactacga
      241 aggtcgctgg acaacggtca aggtcgaaat cgaagacggc attgcctggg tcattctcaa
      301 tcgtccggaa aaacgcaacg ccatgagccc aaccctgaac cgggaaatga tcgacgtgct
      361 ggaaaccctg gaacaggacc cggccgctgg tgtgctggtg ctgaccggcg ccggcgaggc
      421 ctggaccgcc ggcatggacc tcaaggaata cttccgcgag gtggatgccg ggccggagat
      481 ccttcaggag aaaatccgcc gcgaagcctc ccagtggcag tggaaactgc tgcgcatgta
      541 cgccaagccg accatcgcca tggtcaacgg ctggtgcttc ggtggcggct tcagcccgct
      601 ggtggcgtgc gatctggcga tctgcgccga cgaggccacc ttcggcctgt ctgaaatcaa
      661 ctggggcatc ccgccgggca acctggtgag caaggccatg gccgacaccg tgggccatcg
      721 ccagtcgctg tactacatca tgaccggcaa gactttcggc gggcagaaag ccgccgaaat
      781 gggcctggtc aacgacagcg tgcccctggc tcgattgcgt gaggtgacca ttgagctggc
      841 gcgcaacctg ctggagaaaa acccggtggt gctgcgtgcc gccaagcatg gcttcaagcg
      901 ttgccgcgag ctgacctggg agcagaacga agactacctg tacgccaagc tcgatcagtc
      961 gcgcctgttg gacaccgaag gcggccgcga gcagggcatg aagcagtttc tcgacgacaa
     1021 gagcatcaag cccggcctgc aggcgtataa acgctaataa tactagagcc aggcatcaaa
     1081 taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga
     1141 acgctctcta ctagagtcac actggctcac cttcgggtgg gcctttctgc gtttata
//