LOCUS BBa_I769106 1015 bp DNA linear 22-Dec-2024 DEFINITION ech protein generator for vanillin biosynthesis ACCESSION BBa_I769106 VERSION BBa_I769106.1 FEATURES Location/Qualifiers misc_feature 3..19 /label=lac O1 promoter 20..25 /label=-35 misc_feature 26..42 /label=lac O1 promoter 43..48 /label=-10 misc_feature 1..54 /label=BBa_R0011 promoter 1..54 /label=BBa_R0011 misc_feature 68..71 /label=conserved RBS 64..75 /label=BBa_B0034 CDS 82..912 /label=Feruloyl CoA hydratase (ech) CDS 82..912 /label=BBa_I769104 stem_loop 928..947 /label=T7 TE misc_feature 954..954 /label=stop polyA_site 948..961 /label=polya misc_feature 921..961 /label=BBa_B0012 terminator 921..961 /label=BBa_B0012 stem_loop 982..1004 /label=stem_loop misc_feature 970..1015 /label=BBa_B0011 terminator 970..1015 /label=BBa_B0011 ORIGIN 1 aattgtgagc ggataacaat tgacattgtg agcggataac aagatactga gcacatacta 61 gagaaagagg agaaatacta gatgagcaac tacgaaggtc gctggacaac ggtcaaggtc 121 gaaatcgaag acggcattgc ctgggtcatt ctcaatcgtc cggaaaaacg caacgccatg 181 agcccaaccc tgaaccggga aatgatcgac gtgctggaaa ccctggaaca ggacccggcc 241 gctggtgtgc tggtgctgac cggcgccggc gaggcctgga ccgccggcat ggacctcaag 301 gaatacttcc gcgaggtgga tgccgggccg gagatccttc aggagaaaat ccgccgcgaa 361 gcctcccagt ggcagtggaa actgctgcgc atgtacgcca agccgaccat cgccatggtc 421 aacggctggt gcttcggtgg cggcttcagc ccgctggtgg cgtgcgatct ggcgatctgc 481 gccgacgagg ccaccttcgg cctgtctgaa atcaactggg gcatcccgcc gggcaacctg 541 gtgagcaagg ccatggccga caccgtgggc catcgccagt cgctgtacta catcatgacc 601 ggcaagactt tcggcgggca gaaagccgcc gaaatgggcc tggtcaacga cagcgtgccc 661 ctggctcgat tgcgtgaggt gaccattgag ctggcgcgca acctgctgga gaaaaacccg 721 gtggtgctgc gtgccgccaa gcatggcttc aagcgttgcc gcgagctgac ctgggagcag 781 aacgaagact acctgtacgc caagctcgat cagtcgcgcc tgttggacac cgaaggcggc 841 cgcgagcagg gcatgaagca gtttctcgac gacaagagca tcaagcccgg cctgcaggcg 901 tataaacgct gatactagag tcacactggc tcaccttcgg gtgggccttt ctgcgtttat 961 atactagaga gagaatataa aaagccagat tattaatccg gcttttttat tattt //