LOCUS BBa_I8002 11670 bp DNA linear 28-Nov-2024 DEFINITION Booperator: Las/Rhl, monocistronic ACCESSION BBa_I8002 VERSION BBa_I8002.1 FEATURES Location/Qualifiers misc_feature 46..63 /label=OP2 misc_feature 106..125 /label=OP1 promoter 117..122 /label=-35 promoter 140..145 /label=-10 promoter 1..157 /label=BBa_R0079 misc_feature 170..173 /label=conserved RBS 166..177 /label=BBa_B0034 misc_feature 922..927 /label=2 CDS 184..927 /label=cI HK022 misc_feature 889..921 /label=SsrA misc_feature 184..191 /label=OR3 partial misc_feature 184..927 /label=BBa_C0050 misc_feature 928..952 /label=Help:Barcodes CDS 184..927 /label=BBa_C0050 stem_loop 972..1015 /label=stem_loop misc_feature 961..1040 /label=BBa_B0010 terminator 961..1040 /label=BBa_B0010 stem_loop 1056..1075 /label=T7 TE misc_feature 1082..1082 /label=stop polyA_site 1076..1089 /label=polya misc_feature 1049..1089 /label=BBa_B0012 terminator 1049..1089 /label=BBa_B0012 promoter 1105..1110 /label=-35 RBS 1110..1124 /label=OR2 promoter 1127..1132 /label=-10 RBS 1134..1148 /label=OR1 misc_feature 1140..1140 /label=putative misc_feature 1098..1152 /label=BBa_R0050 promoter 1098..1152 /label=BBa_R0050 misc_feature 1165..1168 /label=conserved RBS 1161..1172 /label=BBa_B0034 CDS 1182..1889 /label=cI lambda misc_feature 1890..1922 /label=LVA misc_feature 1929..1953 /label=Help:Barcodes CDS 1179..1928 /label=BBa_C0051 stem_loop 1973..2016 /label=stem_loop misc_feature 1962..2041 /label=BBa_B0010 terminator 1962..2041 /label=BBa_B0010 stem_loop 2057..2076 /label=T7 TE misc_feature 2083..2083 /label=stop polyA_site 2077..2090 /label=polya misc_feature 2050..2090 /label=BBa_B0012 terminator 2050..2090 /label=BBa_B0012 promoter 2136..2141 /label=-10 promoter 2113..2118 /label=-35 misc_feature 2123..2139 /label=OR1 misc_feature 2099..2115 /label=OR2 misc_feature 2099..2147 /label=BBa_R0051 promoter 2099..2147 /label=BBa_R0051 misc_feature 2160..2163 /label=conserved RBS 2156..2167 /label=BBa_B0034 CDS 2174..2776 /label=rhlL misc_feature 2777..2809 /label=LVA misc_feature 2816..2840 /label=Help:Barcodes CDS 2174..2815 /label=BBa_C0070 stem_loop 2860..2903 /label=stem_loop misc_feature 2849..2928 /label=BBa_B0010 terminator 2849..2928 /label=BBa_B0010 stem_loop 2944..2963 /label=T7 TE misc_feature 2970..2970 /label=stop polyA_site 2964..2977 /label=polya misc_feature 2937..2977 /label=BBa_B0012 terminator 2937..2977 /label=BBa_B0012 promoter 3023..3028 /label=-10 promoter 3000..3005 /label=-35 misc_feature 3010..3026 /label=OR1 misc_feature 2986..3002 /label=OR2 misc_feature 2986..3034 /label=BBa_R0051 promoter 2986..3034 /label=BBa_R0051 misc_feature 3047..3050 /label=conserved RBS 3043..3054 /label=BBa_B0034 CDS 3061..3663 /label=LasI misc_feature 3664..3696 /label=LVA misc_feature 3703..3727 /label=Help:Barcodes CDS 3061..3702 /label=BBa_C0078 stem_loop 3747..3790 /label=stem_loop misc_feature 3736..3815 /label=BBa_B0010 terminator 3736..3815 /label=BBa_B0010 stem_loop 3831..3850 /label=T7 TE misc_feature 3857..3857 /label=stop polyA_site 3851..3864 /label=polya misc_feature 3824..3864 /label=BBa_B0012 terminator 3824..3864 /label=BBa_B0012 misc_feature 3918..3935 /label=OP2 misc_feature 3978..3997 /label=OP1 promoter 3989..3994 /label=-35 promoter 4012..4017 /label=-10 promoter 3873..4029 /label=BBa_R0079 misc_feature 4042..4045 /label=conserved RBS 4038..4049 /label=BBa_B0034 CDS 4056..4817 /label=CFP (LVA) misc_feature 4774..4811 /label=SsrA misc_feature 4812..4817 /label=2 misc_feature 4124..4124 /label=A misc_feature 4056..4817 /label=BBa_E0022 misc_feature 4056..4817 /label=BBa_E0022 stem_loop 4837..4880 /label=stem_loop misc_feature 4826..4905 /label=BBa_B0010 terminator 4826..4905 /label=BBa_B0010 stem_loop 4921..4940 /label=T7 TE misc_feature 4947..4947 /label=stop polyA_site 4941..4954 /label=polya misc_feature 4914..4954 /label=BBa_B0012 terminator 4914..4954 /label=BBa_B0012 misc_feature 4963..4982 /label=RhlR promoter 4981..4986 /label=-35 promoter 5004..5009 /label=-10 misc_feature 5015..5015 /label=start promoter 4963..5015 /label=BBa_R0071 misc_feature 5028..5031 /label=conserved RBS 5024..5035 /label=BBa_B0034 CDS 5045..5752 /label=cI lambda misc_feature 5753..5785 /label=LVA misc_feature 5792..5816 /label=Help:Barcodes CDS 5042..5791 /label=BBa_C0051 stem_loop 5836..5879 /label=stem_loop misc_feature 5825..5904 /label=BBa_B0010 terminator 5825..5904 /label=BBa_B0010 stem_loop 5920..5939 /label=T7 TE misc_feature 5946..5946 /label=stop polyA_site 5940..5953 /label=polya misc_feature 5913..5953 /label=BBa_B0012 terminator 5913..5953 /label=BBa_B0012 misc_feature 5962..5981 /label=RhlR promoter 5980..5985 /label=-35 promoter 6003..6008 /label=-10 misc_feature 6014..6014 /label=start promoter 5962..6014 /label=BBa_R0071 misc_feature 6027..6030 /label=conserved RBS 6023..6034 /label=BBa_B0034 CDS 6041..6709 /label=cI 434 misc_feature 6671..6709 /label=LVA misc_feature 6041..6709 /label=BBa_C0052 misc_feature 6710..6734 /label=Help:Barcodes CDS 6041..6709 /label=BBa_C0052 stem_loop 6754..6797 /label=stem_loop misc_feature 6743..6822 /label=BBa_B0010 terminator 6743..6822 /label=BBa_B0010 stem_loop 6838..6857 /label=T7 TE misc_feature 6864..6864 /label=stop polyA_site 6858..6871 /label=polya misc_feature 6831..6871 /label=BBa_B0012 terminator 6831..6871 /label=BBa_B0012 misc_feature 6880..6922 /label=OR2 misc_feature 6887..6900 /label=OR2 misc_feature 6909..6922 /label=OR1 promoter 6880..6885 /label=-35 promoter 6903..6908 /label=-10 promoter 6881..6886 /label=-35 misc_feature 6914..6916 /label=start misc_feature 6880..6925 /label=BBa_R0052 promoter 6880..6925 /label=BBa_R0052 RBS 6934..6946 /label=RBS-3\Weak RBS 6940..6943 /label=RBS misc_feature 6934..6946 /label=BBa_B0032 RBS 6934..6946 /label=BBa_B0032 CDS 6953..7555 /label=LasI misc_feature 7556..7588 /label=LVA misc_feature 7595..7619 /label=Help:Barcodes CDS 6953..7594 /label=BBa_C0078 stem_loop 7639..7682 /label=stem_loop misc_feature 7628..7707 /label=BBa_B0010 terminator 7628..7707 /label=BBa_B0010 stem_loop 7723..7742 /label=T7 TE misc_feature 7749..7749 /label=stop polyA_site 7743..7756 /label=polya misc_feature 7716..7756 /label=BBa_B0012 terminator 7716..7756 /label=BBa_B0012 misc_feature 7765..7784 /label=RhlR promoter 7783..7788 /label=-35 promoter 7806..7811 /label=-10 misc_feature 7817..7817 /label=start promoter 7765..7817 /label=BBa_R0071 misc_feature 7830..7833 /label=conserved RBS 7826..7837 /label=BBa_B0034 CDS 7844..8605 /label=YFP (LVA) misc_feature 8600..8605 /label=2 misc_feature 8561..8599 /label=SsrA misc_feature 8085..8085 /label=A (Q->L) misc_feature 7844..8605 /label=BBa_E0032 misc_feature 7844..8605 /label=BBa_E0032 stem_loop 8625..8668 /label=stem_loop misc_feature 8614..8693 /label=BBa_B0010 terminator 8614..8693 /label=BBa_B0010 stem_loop 8709..8728 /label=T7 TE misc_feature 8735..8735 /label=stop polyA_site 8729..8742 /label=polya misc_feature 8702..8742 /label=BBa_B0012 terminator 8702..8742 /label=BBa_B0012 misc_feature 8751..8769 /label=TetR 1 misc_feature 8751..8804 /label=BBa_R0040 promoter 8770..8775 /label=-35 misc_feature 8776..8794 /label=TetR 2 promoter 8793..8798 /label=-10 promoter 8751..8804 /label=BBa_R0040 misc_feature 8817..8820 /label=conserved RBS 8813..8824 /label=BBa_B0034 CDS 8834..9450 /label=tetR misc_feature 9451..9484 /label=SsrA misc_feature 9491..9515 /label=Help:Barcodes CDS 8831..9490 /label=BBa_C0040 stem_loop 9535..9578 /label=stem_loop misc_feature 9524..9603 /label=BBa_B0010 terminator 9524..9603 /label=BBa_B0010 stem_loop 9619..9638 /label=T7 TE misc_feature 9645..9645 /label=stop polyA_site 9639..9652 /label=polya misc_feature 9612..9652 /label=BBa_B0012 terminator 9612..9652 /label=BBa_B0012 misc_feature 9661..9679 /label=TetR 1 misc_feature 9661..9714 /label=BBa_R0040 promoter 9680..9685 /label=-35 misc_feature 9686..9704 /label=TetR 2 promoter 9703..9708 /label=-10 promoter 9661..9714 /label=BBa_R0040 misc_feature 9727..9730 /label=conserved RBS 9723..9734 /label=BBa_B0034 CDS 9741..10463 /label=rhlR misc_feature 10464..10496 /label=LVA misc_feature 10503..10527 /label=Help:Barcodes CDS 9741..10502 /label=BBa_C0071 stem_loop 10547..10590 /label=stem_loop misc_feature 10536..10615 /label=BBa_B0010 terminator 10536..10615 /label=BBa_B0010 stem_loop 10631..10650 /label=T7 TE misc_feature 10657..10657 /label=stop polyA_site 10651..10664 /label=polya misc_feature 10624..10664 /label=BBa_B0012 terminator 10624..10664 /label=BBa_B0012 misc_feature 10673..10691 /label=TetR 1 misc_feature 10673..10726 /label=BBa_R0040 promoter 10692..10697 /label=-35 misc_feature 10698..10716 /label=TetR 2 promoter 10715..10720 /label=-10 promoter 10673..10726 /label=BBa_R0040 misc_feature 10739..10742 /label=conserved RBS 10735..10746 /label=BBa_B0034 CDS 10753..11469 /label=lasR misc_feature 11470..11502 /label=LVA misc_feature 11232..11232 /label=G misc_feature 11509..11533 /label=Help:Barcodes CDS 10753..11508 /label=BBa_C0079 stem_loop 11553..11596 /label=stem_loop misc_feature 11542..11621 /label=BBa_B0010 terminator 11542..11621 /label=BBa_B0010 stem_loop 11637..11656 /label=T7 TE misc_feature 11663..11663 /label=stop polyA_site 11657..11670 /label=polya misc_feature 11630..11670 /label=BBa_B0012 terminator 11630..11670 /label=BBa_B0012 ORIGIN 1 gcccctcgct gagcgcgtcc cggagctggg ggcaacctag ctgccacctg cttttctgct 61 agctattcca gcgaaaacat acagatttcc ggcgaaatca aggctacctg ccagttctgg 121 caggtttggc cgcgggttct ttttggtaca cgaaagctac tagagaaaga ggagaaatac 181 tagatggttc aacagaaaga gcgtgaaact ttctcgcaga ggcttgcgct ggcctgtgat 241 aaagcgggat tacctttgca tggtaggcag gctgatttag ctgtcaggct taaggtcaca 301 ccaaaagcca ttagtaaatg gttcaacggg gagtcaatac caagaaaaga caagatggaa 361 tctctggctt cggtgctggg aactactgct gcatatctgc atggctatgc tgatgatgac 421 ggtatcacgg taaatcatct atcaagatca aatgattatt atcgtgttga tgtattggat 481 gttcaggcga gcgccgggcc aggaaccatg gtttccaatg aatttataga aaagataaga 541 gcaattgaat atacgaccga gcaggcaaga attttattta atggaaggcc acaggaaagc 601 gtaaaagtca tcacggttcg cggtgacagc atggagggaa ccatcaatcc gggagatgag 661 atctttgttg atgtatccat aacctgtttt gatggcgatg gcatttatgt gtttgtatac 721 gggaaaacaa tgcacgttaa gcgcctgcaa atgcaaaaga acaggcttgc cgtcatctct 781 gacaatgccg cttatgatcg atggtacata gaagaaggtg aagaagagca acttcacatt 841 ctagccaaag tcctcattag gcagtcaatc gattacaagc gattcggagc tgcaaacgac 901 gaaaactacg ctttagtagc ttaataaccc tgatagtgct agtgtagatc cctactagag 961 ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt 1021 gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct 1081 gcgtttatat actagagtta ggtattgact gtactatcag ttccgtcata atatgaacca 1141 taagttcacc actactagag aaagaggaga aatactagat gagcacaaaa aagaaaccat 1201 taacacaaga gcagcttgag gacgcacgtc gccttaaagc aatttatgaa aaaaagaaaa 1261 atgaacttgg cttatcccag gaatctgtcg cagacaagat ggggatgggg cagtcaggcg 1321 ttggtgcttt atttaatggc atcaatgcat taaatgctta taacgccgca ttgcttgcaa 1381 aaattctcaa agttagcgtt gaagaattta gcccttcaat cgccagagaa atctacgaga 1441 tgtatgaagc ggttagtatg cagccgtcac ttagaagtga gtatgagtac cctgtttttt 1501 ctcatgttca ggcagggatg ttctcacctg agcttagaac ctttaccaaa ggtgatgcgg 1561 agagatgggt aagcacaacc aaaaaagcca gtgattctgc attctggctt gaggttgaag 1621 gtaattccat gaccgcacca acaggctcca agccaagctt tcctgacgga atgttaattc 1681 tcgttgaccc tgagcaggct gttgagccag gtgatttctg catagccaga cttgggggtg 1741 atgagtttac cttcaagaaa ctgatcaggg atagcggtca ggtgttttta caaccactaa 1801 acccacagta cccaatgatc ccatgcaatg agagttgttc cgttgtgggg aaagttatcg 1861 ctagtcagtg gcctgaagag acgtttggcg ctgcaaacga cgaaaactac gctttagtag 1921 cttaataacg ctgatagtgc tagtgtagat cgctactaga gccaggcatc aaataaaacg 1981 aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct 2041 ctactagagt cacactggct caccttcggg tgggcctttc tgcgtttata tactagagta 2101 acaccgtgcg tgttgactat tttacctctg gcggtgataa tggttgctac tagagaaaga 2161 ggagaaatac tagatgatcg aactgctgtc cgaatccctg gaaggtctgt ccgctgctat 2221 gatcgctgaa ctgggtcgtt accgtcacca ggttttcatc gaaaaactgg gttgggacgt 2281 tgtttccacc tcccgtgttc gtgaccagga gttcgaccag ttcgaccacc cgcagacccg 2341 ttacatcgtt gctatgtccc gtcagggtat ctgcggttgc gctcgtctgc tgccgaccac 2401 cgacgcttac ctgctgaaag acgttttcgc ttacctgtgc tccgaaaccc cgccgtccga 2461 cccgtccgtt tgggaactgt cccgttacgc tgcttccgct gctgacgacc cgcagctggc 2521 tatgaaaatc ttctggtcct ccctccagtg cgcttggtac ctgggtgctt cctccgttgt 2581 tgctgttacc accaccgcta tggaacgtta cttcgttcgt aacggtgtta tcctccagcg 2641 tctgggtccg ccgcagaaag ttaaaggtga aaccctggtt gctatctcct tcccggctta 2701 ccaggaacgt ggtctggaaa tgctgctgcg ttaccacccg gaatggctcc agggtgttcc 2761 gctgtccatg gctgttgctg caaacgacga aaactacgct ttagtagctt aataactctg 2821 atagtgctag tgtagatctc tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg 2881 aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcta ctagagtcac 2941 actggctcac cttcgggtgg gcctttctgc gtttatatac tagagtaaca ccgtgcgtgt 3001 tgactatttt acctctggcg gtgataatgg ttgctactag agaaagagga gaaatactag 3061 atgatcgttc agatcggtcg tcgtgaagag ttcgacaaaa aactgctggg tgaaatgcac 3121 aaactgcgtg ctcaggtttt caaagaacgt aaaggttggg acgtttccgt tatcgacgaa 3181 atggaaatcg acggttacga cgctctgtcc ccgtactaca tgctgatcca ggaagacacc 3241 ccggaagctc aggttttcgg ttgctggcgt atcttcgaca ccaccggtcc gtacatgctg 3301 aaaaacacct tcccggaact gctgcacggt aaagaagctc cgtgctcccc gcacatctgg 3361 gaactgtccc gtttcgctat caactccggt cagaaaggtt ccctgggttt ctccgactgc 3421 accctggaag ctatgcgtgc tctggctcgt tactccttgc agaacgacat ccagaccctg 3481 gttaccgtta ccaccgttgg tgttgaaaaa atgatgatcc gtgctggtct ggacgtttcc 3541 cgtttcggtc cgcacctgaa aatcggtatc gaacgtgctg ttgctctgcg tatcgaactg 3601 aacgctaaaa cccagatcgc tctgtacggt ggtgttctgg ttgaacagcg tctggctgtt 3661 tccgctgcta acgacgaaaa ctacgctctg gttgcttaat aactctgata gtgctagtgt 3721 agatctctac tagagccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct 3781 ttcgttttat ctgttgtttg tcggtgaacg ctctctacta gagtcacact ggctcacctt 3841 cgggtgggcc tttctgcgtt tatatactag aggcccctcg ctgagcgcgt cccggagctg 3901 ggggcaacct agctgccacc tgcttttctg ctagctattc cagcgaaaac atacagattt 3961 ccggcgaaat caaggctacc tgccagttct ggcaggtttg gccgcgggtt ctttttggta 4021 cacgaaagct actagagaaa gaggagaaat actagatggt gagcaagggc gaggagctgt 4081 tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtgaacggc cacaagttca 4141 gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagttcatct 4201 gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg acctggggcg 4261 tgcagtgctt cagccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca 4321 tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga 4381 cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca 4441 tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac tacatcagcc 4501 acaacgtcta tatcaccgcc gacaagcaga agaacggcat caaggccaac ttcaagatcc 4561 gccacaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag aacaccccca 4621 tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagcacccag tccgccctga 4681 gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg 4741 ggatcactct cggcatggac gagctgtaca agaggcctgc tgcaaacgac gaaaactacg 4801 ctttagtagc ttaataatac tagagccagg catcaaataa aacgaaaggc tcagtcgaaa 4861 gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctctacta gagtcacact 4921 ggctcacctt cgggtgggcc tttctgcgtt tatatactag agtcctgtga aatctggcag 4981 ttaccgttag ctttcgaatt ggctaaaaag tgttctacta gagaaagagg agaaatacta 5041 gatgagcaca aaaaagaaac cattaacaca agagcagctt gaggacgcac gtcgccttaa 5101 agcaatttat gaaaaaaaga aaaatgaact tggcttatcc caggaatctg tcgcagacaa 5161 gatggggatg gggcagtcag gcgttggtgc tttatttaat ggcatcaatg cattaaatgc 5221 ttataacgcc gcattgcttg caaaaattct caaagttagc gttgaagaat ttagcccttc 5281 aatcgccaga gaaatctacg agatgtatga agcggttagt atgcagccgt cacttagaag 5341 tgagtatgag taccctgttt tttctcatgt tcaggcaggg atgttctcac ctgagcttag 5401 aacctttacc aaaggtgatg cggagagatg ggtaagcaca accaaaaaag ccagtgattc 5461 tgcattctgg cttgaggttg aaggtaattc catgaccgca ccaacaggct ccaagccaag 5521 ctttcctgac ggaatgttaa ttctcgttga ccctgagcag gctgttgagc caggtgattt 5581 ctgcatagcc agacttgggg gtgatgagtt taccttcaag aaactgatca gggatagcgg 5641 tcaggtgttt ttacaaccac taaacccaca gtacccaatg atcccatgca atgagagttg 5701 ttccgttgtg gggaaagtta tcgctagtca gtggcctgaa gagacgtttg gcgctgcaaa 5761 cgacgaaaac tacgctttag tagcttaata acgctgatag tgctagtgta gatcgctact 5821 agagccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc 5881 tgttgtttgt cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct 5941 ttctgcgttt atatactaga gtcctgtgaa atctggcagt taccgttagc tttcgaattg 6001 gctaaaaagt gttctactag agaaagagga gaaatactag atgagtattt cttccagggt 6061 aaaaagcaaa agaatccagc ttggacttaa ccaggctgaa cttgctcaaa aggtggggac 6121 tacccagcag tctatagagc agctcgaaaa cggtaaaact aagcgaccac gctttttacc 6181 agaacttgcg tcagctcttg gcgtaagtgt tgactggctg ctcaatggca cctctgattc 6241 gaatgttaga tttgttgggc acgttgagcc caaagggaaa tatccattga ttagcatggt 6301 tagagctggt tcgtggtgtg aagcttgtga accctacgat atcaaggaca ttgatgaatg 6361 gtatgacagt gacgttaact tattaggcaa tggattctgg ctgaaggttg aaggtgattc 6421 catgacctca cctgtaggtc aaagcatccc tgaaggtcat atggtgttag tagatactgg 6481 acgggagcca gtgaatggaa gccttgttgt agccaaactg actgacgcga acgaagcaac 6541 attcaagaaa ctggtcatag atggcggtca gaagtacctg aaaggcctga atccttcatg 6601 gcctatgact cctatcaacg gaaactgcaa gattatcggt gttgtcgtgg aagcgagggt 6661 aaaattcgta gctgcaaacg acgaaaacta cgctttagta gcttaataac tctgatagtg 6721 ctagtgtaga tctctactag agccaggcat caaataaaac gaaaggctca gtcgaaagac 6781 tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tctactagag tcacactggc 6841 tcaccttcgg gtgggccttt ctgcgtttat atactagagt tgacaaacaa gatacattgt 6901 atgaaaatac aagaaagttt gttgatacta gagtcacaca ggaaagtact agatgatcgt 6961 tcagatcggt cgtcgtgaag agttcgacaa aaaactgctg ggtgaaatgc acaaactgcg 7021 tgctcaggtt ttcaaagaac gtaaaggttg ggacgtttcc gttatcgacg aaatggaaat 7081 cgacggttac gacgctctgt ccccgtacta catgctgatc caggaagaca ccccggaagc 7141 tcaggttttc ggttgctggc gtatcttcga caccaccggt ccgtacatgc tgaaaaacac 7201 cttcccggaa ctgctgcacg gtaaagaagc tccgtgctcc ccgcacatct gggaactgtc 7261 ccgtttcgct atcaactccg gtcagaaagg ttccctgggt ttctccgact gcaccctgga 7321 agctatgcgt gctctggctc gttactcctt gcagaacgac atccagaccc tggttaccgt 7381 taccaccgtt ggtgttgaaa aaatgatgat ccgtgctggt ctggacgttt cccgtttcgg 7441 tccgcacctg aaaatcggta tcgaacgtgc tgttgctctg cgtatcgaac tgaacgctaa 7501 aacccagatc gctctgtacg gtggtgttct ggttgaacag cgtctggctg tttccgctgc 7561 taacgacgaa aactacgctc tggttgctta ataactctga tagtgctagt gtagatctct 7621 actagagcca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt 7681 atctgttgtt tgtcggtgaa cgctctctac tagagtcaca ctggctcacc ttcgggtggg 7741 cctttctgcg tttatatact agagtcctgt gaaatctggc agttaccgtt agctttcgaa 7801 ttggctaaaa agtgttctac tagagaaaga ggagaaatac tagatggtga gcaagggcga 7861 ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca 7921 caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa 7981 gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga ccaccttcgg 8041 ctacggcctg caatgcttcg cccgctaccc cgaccacatg aagctgcacg acttcttcaa 8101 gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa 8161 ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct 8221 gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta 8281 caacagccac aacgtctata tcatggccga caagcagaag aacggcatca aggtgaactt 8341 caagatccgc cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa 8401 cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga gctaccagtc 8461 cgccctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac 8521 cgccgccggg atcactctcg gcatggacga gctgtacaag aggcctgctg caaacgacga 8581 aaactacgct ttagtagctt aataatacta gagccaggca tcaaataaaa cgaaaggctc 8641 agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctctactaga 8701 gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta tatactagag tccctatcag 8761 tgatagagat tgacatccct atcagtgata gagatactga gcactactag agaaagagga 8821 gaaatactag atgtccagat tagataaaag taaagtgatt aacagcgcat tagagctgct 8881 taatgaggtc ggaatcgaag gtttaacaac ccgtaaactc gcccagaagc taggtgtaga 8941 gcagcctaca ttgtattggc atgtaaaaaa taagcgggct ttgctcgacg ccttagccat 9001 tgagatgtta gataggcacc atactcactt ttgcccttta gaaggggaaa gctggcaaga 9061 ttttttacgt aataacgcta aaagttttag atgtgcttta ctaagtcatc gcgatggagc 9121 aaaagtacat ttaggtacac ggcctacaga aaaacagtat gaaactctcg aaaatcaatt 9181 agccttttta tgccaacaag gtttttcact agagaatgca ttatatgcac tcagcgctgt 9241 ggggcatttt actttaggtt gcgtattgga agatcaagag catcaagtcg ctaaagaaga 9301 aagggaaaca cctactactg atagtatgcc gccattatta cgacaagcta tcgaattatt 9361 tgatcaccaa ggtgcagagc cagccttctt attcggcctt gaattgatca tatgcggatt 9421 agaaaaacaa cttaaatgtg aaagtgggtc cgctgcaaac gacgaaaact acgctttagt 9481 agcttaataa cactgatagt gctagtgtag atcactacta gagccaggca tcaaataaaa 9541 cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct 9601 ctctactaga gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta tatactagag 9661 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag 9721 agaaagagga gaaatactag atgaggaatg acggaggctt tttgctgtgg tgggacggtt 9781 tgcgtagcga gatgcagccg atccacgaca gccagggcgt gttcgccgtc ctggaaaagg 9841 aagtgcggcg cctgggcttc gattactacg cctatggcgt gcgccacacg attcccttca 9901 cccggccgaa gaccgaggtc catggcacct atcccaaggc ctggctggag cgataccaga 9961 tgcagaacta cggggccgtg gatccggcga tcctcaacgg cctgcgctcc tcggaaatgg 10021 tggtctggag cgacagcctg ttcgaccaga gccggatgct ctggaacgag gctcgcgatt 10081 ggggcctctg tgtcggcgcg accttgccga tccgcgcgcc gaacaatttg ctcagcgtgc 10141 tttccgtggc gcgcgaccag cagaacatct ccagcttcga gcgcgaggaa atccgcctgc 10201 ggctgcgttg catgatcgag ttgctgaccc agaagctgac cgacctggag catccgatgc 10261 tgatgtccaa cccggtctgc ctgagccatc gcgaacgcga gatcctgcaa tggaccgccg 10321 acggcaagag ttccggggaa atcgccatca tcctgagcat ctccgagagc acggtgaact 10381 tccaccacaa gaacatccag aagaagttcg acgcgccgaa caagacgctg gctgccgcct 10441 acgccgcggc gctgggtctc atcgctgcaa acgacgaaaa ctacgcttta gtagcttaat 10501 aacactgata gtgctagtgt agatcactac tagagccagg catcaaataa aacgaaaggc 10561 tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctctacta 10621 gagtcacact ggctcacctt cgggtgggcc tttctgcgtt tatatactag agtccctatc 10681 agtgatagag attgacatcc ctatcagtga tagagatact gagcactact agagaaagag 10741 gagaaatact agatggcctt ggttgacggt tttcttgagc tggaacgctc aagtggaaaa 10801 ttggagtgga gcgccatcct ccagaagatg gcgagcgacc ttggattctc gaagatcctg 10861 ttcggcctgt tgcctaagga cagccaggac tacgagaacg ccttcatcgt cggcaactac 10921 ccggccgcct ggcgcgagca ttacgaccgg gctggctacg cgcgggtcga cccgacggtc 10981 agtcactgta cccagagcgt actgccgatt ttctgggaac cgtccatcta ccagacgcga 11041 aagcagcacg agttcttcga ggaagcctcg gccgccggcc tggtgtatgg gctgaccatg 11101 ccgctgcatg gtgctcgcgg cgaactcggc gcgctgagcc tcagcgtgga agcggaaaac 11161 cgggccgagg ccaaccgttt catagagtcg gtcctgccga ccctgtggat gctcaaggac 11221 tacgcactgc aaagcggtgc cggactggcc ttcgaacatc cggtcagcaa accggtggtt 11281 ctgaccagcc gggagaagga agtgttgcag tggtgcgcca tcggcaagac cagttgggag 11341 atatcggtta tctgcaactg ctcggaagcc aatgtgaact tccatatggg aaatattcgg 11401 cggaagttcg gtgtgacctc ccgccgcgta gcggccatta tggccgttaa tttgggtctt 11461 attactctcg ctgcaaacga cgaaaactac gctttagtag cttaataaca ctgatagtgc 11521 tagtgtagat cactactaga gccaggcatc aaataaaacg aaaggctcag tcgaaagact 11581 gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct ctactagagt cacactggct 11641 caccttcggg tgggcctttc tgcgtttata //