LOCUS BBa_I8003 6698 bp DNA linear 09-May-2024 DEFINITION Booperator: aiia/LuxR/RhlR, polycistronic ACCESSION BBa_I8003 VERSION BBa_I8003.1 FEATURES Location/Qualifiers misc_feature 1..20 /label=LuxR/HSL promoter 20..25 /label=-35 promoter 42..47 /label=-10 misc_feature 53..53 /label=start misc_feature 1..55 /label=BBa_R0062 promoter 1..55 /label=BBa_R0062 misc_feature 68..71 /label=conserved RBS 64..75 /label=BBa_B0034 CDS 82..843 /label=CFP (LVA) misc_feature 800..837 /label=SsrA misc_feature 838..843 /label=2 misc_feature 150..150 /label=A misc_feature 82..843 /label=BBa_E0022 misc_feature 82..843 /label=BBa_E0022 misc_feature 856..859 /label=conserved RBS 852..863 /label=BBa_B0034 CDS 870..1472 /label=rhlL misc_feature 1473..1505 /label=LVA misc_feature 1512..1536 /label=Help:Barcodes CDS 870..1511 /label=BBa_C0070 misc_feature 1549..1552 /label=conserved RBS 1545..1556 /label=BBa_B0034 misc_feature 2142..2173 /label=LVA CDS 1563..2141 /label=luxI misc_feature 1563..2180 /label=BBa_C0061 misc_feature 2181..2205 /label=Help:Barcodes CDS 1563..2180 /label=BBa_C0061 stem_loop 2225..2268 /label=stem_loop misc_feature 2214..2293 /label=BBa_B0010 terminator 2214..2293 /label=BBa_B0010 stem_loop 2309..2328 /label=T7 TE misc_feature 2335..2335 /label=stop polyA_site 2329..2342 /label=polya misc_feature 2302..2342 /label=BBa_B0012 terminator 2302..2342 /label=BBa_B0012 misc_feature 2351..2370 /label=RhlR promoter 2369..2374 /label=-35 promoter 2392..2397 /label=-10 misc_feature 2403..2403 /label=start promoter 2351..2403 /label=BBa_R0071 misc_feature 2416..2419 /label=conserved RBS 2412..2423 /label=BBa_B0034 CDS 2430..3191 /label=YFP (LVA) misc_feature 3186..3191 /label=2 misc_feature 3147..3185 /label=SsrA misc_feature 2671..2671 /label=A (Q->L) misc_feature 2430..3191 /label=BBa_E0032 misc_feature 2430..3191 /label=BBa_E0032 misc_feature 3204..3207 /label=conserved RBS 3200..3211 /label=BBa_B0034 misc_feature 3218..3220 /label=start misc_feature 4001..4006 /label=2 misc_feature 3968..4000 /label=LVA CDS 3218..3967 /label=aiiA misc_feature 3218..4006 /label=BBa_C0060 misc_feature 4007..4031 /label=Help:Barcodes CDS 3218..4006 /label=BBa_C0060 stem_loop 4051..4094 /label=stem_loop misc_feature 4040..4119 /label=BBa_B0010 terminator 4040..4119 /label=BBa_B0010 stem_loop 4135..4154 /label=T7 TE misc_feature 4161..4161 /label=stop polyA_site 4155..4168 /label=polya misc_feature 4128..4168 /label=BBa_B0012 terminator 4128..4168 /label=BBa_B0012 misc_feature 4177..4195 /label=TetR 1 misc_feature 4177..4230 /label=BBa_R0040 promoter 4196..4201 /label=-35 misc_feature 4202..4220 /label=TetR 2 promoter 4219..4224 /label=-10 promoter 4177..4230 /label=BBa_R0040 misc_feature 4243..4246 /label=conserved RBS 4239..4250 /label=BBa_B0034 CDS 4260..4876 /label=tetR misc_feature 4877..4910 /label=SsrA misc_feature 4917..4941 /label=Help:Barcodes CDS 4257..4916 /label=BBa_C0040 misc_feature 4954..4957 /label=conserved RBS 4950..4961 /label=BBa_B0034 CDS 4968..5690 /label=rhlR misc_feature 5691..5723 /label=LVA misc_feature 5730..5754 /label=Help:Barcodes CDS 4968..5729 /label=BBa_C0071 misc_feature 5767..5770 /label=conserved RBS 5763..5774 /label=BBa_B0034 misc_feature 5781..5782 /label=prefix misc_feature 5954..5954 /label=T misc_feature 6272..6272 /label=A CDS 5781..6530 /label=luxR misc_feature 5781..6536 /label=BBa_C0062 misc_feature 6537..6561 /label=Help:Barcodes CDS 5781..6536 /label=BBa_C0062 stem_loop 6581..6624 /label=stem_loop misc_feature 6570..6649 /label=BBa_B0010 terminator 6570..6649 /label=BBa_B0010 stem_loop 6665..6684 /label=T7 TE misc_feature 6691..6691 /label=stop polyA_site 6685..6698 /label=polya misc_feature 6658..6698 /label=BBa_B0012 terminator 6658..6698 /label=BBa_B0012 ORIGIN 1 acctgtagga tcgtacaggt ttacgcaaga aaatggtttg ttatagtcga ataaatacta 61 gagaaagagg agaaatacta gatggtgagc aagggcgagg agctgttcac cggggtggtg 121 cccatcctgg tcgagctgga cggcgacgtg aacggccaca agttcagcgt gtccggcgag 181 ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag 241 ctgcccgtgc cctggcccac cctcgtgacc accctgacct ggggcgtgca gtgcttcagc 301 cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac 361 gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg 421 aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag 481 gacggcaaca tcctggggca caagctggag tacaactaca tcagccacaa cgtctatatc 541 accgccgaca agcagaagaa cggcatcaag gccaacttca agatccgcca caacatcgag 601 gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc 661 gtgctgctgc ccgacaacca ctacctgagc acccagtccg ccctgagcaa agaccccaac 721 gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc 781 atggacgagc tgtacaagag gcctgctgca aacgacgaaa actacgcttt agtagcttaa 841 taatactaga gaaagaggag aaatactaga tgatcgaact gctgtccgaa tccctggaag 901 gtctgtccgc tgctatgatc gctgaactgg gtcgttaccg tcaccaggtt ttcatcgaaa 961 aactgggttg ggacgttgtt tccacctccc gtgttcgtga ccaggagttc gaccagttcg 1021 accacccgca gacccgttac atcgttgcta tgtcccgtca gggtatctgc ggttgcgctc 1081 gtctgctgcc gaccaccgac gcttacctgc tgaaagacgt tttcgcttac ctgtgctccg 1141 aaaccccgcc gtccgacccg tccgtttggg aactgtcccg ttacgctgct tccgctgctg 1201 acgacccgca gctggctatg aaaatcttct ggtcctccct ccagtgcgct tggtacctgg 1261 gtgcttcctc cgttgttgct gttaccacca ccgctatgga acgttacttc gttcgtaacg 1321 gtgttatcct ccagcgtctg ggtccgccgc agaaagttaa aggtgaaacc ctggttgcta 1381 tctccttccc ggcttaccag gaacgtggtc tggaaatgct gctgcgttac cacccggaat 1441 ggctccaggg tgttccgctg tccatggctg ttgctgcaaa cgacgaaaac tacgctttag 1501 tagcttaata actctgatag tgctagtgta gatctctact agagaaagag gagaaatact 1561 agatgactat aatgataaaa aaatcggatt ttttggcaat tccatcggag gagtataaag 1621 gtattctaag tcttcgttat caagtgttta agcaaagact tgagtgggac ttagttgtag 1681 aaaataacct tgaatcagat gagtatgata actcaaatgc agaatatatt tatgcttgtg 1741 atgatactga aaatgtaagt ggatgctggc gtttattacc tacaacaggt gattatatgc 1801 tgaaaagtgt ttttcctgaa ttgcttggtc aacagagtgc tcccaaagat cctaatatag 1861 tcgaattaag tcgttttgct gtaggtaaaa atagctcaaa gataaataac tctgctagtg 1921 aaattacaat gaaactattt gaagctatat ataaacacgc tgttagtcaa ggtattacag 1981 aatatgtaac agtaacatca acagcaatag agcgattttt aaagcgtatt aaagttcctt 2041 gtcatcgtat tggagacaaa gaaattcatg tattaggtga tactaaatcg gttgtattgt 2101 ctatgcctat taatgaacag tttaaaaaag cagtcttaaa tgctgcaaac gacgaaaact 2161 acgctttagt agcttaataa ctctgatagt gctagtgtag atctctacta gagccaggca 2221 tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc 2281 ggtgaacgct ctctactaga gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta 2341 tatactagag tcctgtgaaa tctggcagtt accgttagct ttcgaattgg ctaaaaagtg 2401 ttctactaga gaaagaggag aaatactaga tggtgagcaa gggcgaggag ctgttcaccg 2461 gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt 2521 ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagttc atctgcacca 2581 ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cttcggctac ggcctgcaat 2641 gcttcgcccg ctaccccgac cacatgaagc tgcacgactt cttcaagtcc gccatgcccg 2701 aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg 2761 ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact 2821 tcaaggagga cggcaacatc ctggggcaca agctggagta caactacaac agccacaacg 2881 tctatatcat ggccgacaag cagaagaacg gcatcaaggt gaacttcaag atccgccaca 2941 acatcgagga cggcagcgtg cagctcgccg accactacca gcagaacacc cccatcggcg 3001 acggccccgt gctgctgccc gacaaccact acctgagcta ccagtccgcc ctgagcaaag 3061 accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca 3121 ctctcggcat ggacgagctg tacaagaggc ctgctgcaaa cgacgaaaac tacgctttag 3181 tagcttaata atactagaga aagaggagaa atactagatg acagtaaaga agctttattt 3241 cgtcccagca ggtcgttgta tgttggatca ttcgtctgtt aatagtacat taacaccagg 3301 agaattatta gacttaccgg tttggtgtta tcttttggag actgaagaag gacctatttt 3361 agtagataca ggtatgccag aaagtgcagt taataatgaa ggtcttttta acggtacatt 3421 tgtcgaaggg caggttttac cgaaaatgac tgaagaagat agaatcgtga atattttaaa 3481 acgggttggt tatgagccgg aagaccttct ttatattatt agttctcact tgcattttga 3541 tcatgcagga ggaaatggcg cttttataaa tacaccaatc attgtacagc gtgctgaata 3601 tgaggcggcg cagcatagcg aagaatattt gaaagaatgt atattgccga atttaaacta 3661 caaaatcatt gaaggtgatt atgaagtcgt accaggagtt caattattgc atacaccagg 3721 ccatactcca gggcatcaat cgctattaat tgagacagaa aaatccggtc ctgtattatt 3781 aacgattgat gcatcgtata cgaaagagaa ttttgaaaat gaagtgccat ttgcgggatt 3841 tgattcagaa ttagctttat cttcaattaa acgtttaaaa gaagtggtga tgaaagagaa 3901 gccgattgtt ttctttggac atgatataga gcaggaaagg ggatgtaaag tgttccctga 3961 atatatagct gcaaacgacg aaaactacgc tttagtagct taataacgct gatagtgcta 4021 gtgtagatcg ctactagagc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg 4081 gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca cactggctca 4141 ccttcgggtg ggcctttctg cgtttatata ctagagtccc tatcagtgat agagattgac 4201 atccctatca gtgatagaga tactgagcac tactagagaa agaggagaaa tactagatgt 4261 ccagattaga taaaagtaaa gtgattaaca gcgcattaga gctgcttaat gaggtcggaa 4321 tcgaaggttt aacaacccgt aaactcgccc agaagctagg tgtagagcag cctacattgt 4381 attggcatgt aaaaaataag cgggctttgc tcgacgcctt agccattgag atgttagata 4441 ggcaccatac tcacttttgc cctttagaag gggaaagctg gcaagatttt ttacgtaata 4501 acgctaaaag ttttagatgt gctttactaa gtcatcgcga tggagcaaaa gtacatttag 4561 gtacacggcc tacagaaaaa cagtatgaaa ctctcgaaaa tcaattagcc tttttatgcc 4621 aacaaggttt ttcactagag aatgcattat atgcactcag cgctgtgggg cattttactt 4681 taggttgcgt attggaagat caagagcatc aagtcgctaa agaagaaagg gaaacaccta 4741 ctactgatag tatgccgcca ttattacgac aagctatcga attatttgat caccaaggtg 4801 cagagccagc cttcttattc ggccttgaat tgatcatatg cggattagaa aaacaactta 4861 aatgtgaaag tgggtccgct gcaaacgacg aaaactacgc tttagtagct taataacact 4921 gatagtgcta gtgtagatca ctactagaga aagaggagaa atactagatg aggaatgacg 4981 gaggcttttt gctgtggtgg gacggtttgc gtagcgagat gcagccgatc cacgacagcc 5041 agggcgtgtt cgccgtcctg gaaaaggaag tgcggcgcct gggcttcgat tactacgcct 5101 atggcgtgcg ccacacgatt cccttcaccc ggccgaagac cgaggtccat ggcacctatc 5161 ccaaggcctg gctggagcga taccagatgc agaactacgg ggccgtggat ccggcgatcc 5221 tcaacggcct gcgctcctcg gaaatggtgg tctggagcga cagcctgttc gaccagagcc 5281 ggatgctctg gaacgaggct cgcgattggg gcctctgtgt cggcgcgacc ttgccgatcc 5341 gcgcgccgaa caatttgctc agcgtgcttt ccgtggcgcg cgaccagcag aacatctcca 5401 gcttcgagcg cgaggaaatc cgcctgcggc tgcgttgcat gatcgagttg ctgacccaga 5461 agctgaccga cctggagcat ccgatgctga tgtccaaccc ggtctgcctg agccatcgcg 5521 aacgcgagat cctgcaatgg accgccgacg gcaagagttc cggggaaatc gccatcatcc 5581 tgagcatctc cgagagcacg gtgaacttcc accacaagaa catccagaag aagttcgacg 5641 cgccgaacaa gacgctggct gccgcctacg ccgcggcgct gggtctcatc gctgcaaacg 5701 acgaaaacta cgctttagta gcttaataac actgatagtg ctagtgtaga tcactactag 5761 agaaagagga gaaatactag atgaaaaaca taaatgccga cgacacatac agaataatta 5821 ataaaattaa agcttgtaga agcaataatg atattaatca atgcttatct gatatgacta 5881 aaatggtaca ttgtgaatat tatttactcg cgatcattta tcctcattct atggttaaat 5941 ctgatatttc aatcctagat aattacccta aaaaatggag gcaatattat gatgacgcta 6001 atttaataaa atatgatcct atagtagatt attctaactc caatcattca ccaattaatt 6061 ggaatatatt tgaaaacaat gctgtaaata aaaaatctcc aaatgtaatt aaagaagcga 6121 aaacatcagg tcttatcact gggtttagtt tccctattca tacggctaac aatggcttcg 6181 gaatgcttag ttttgcacat tcagaaaaag acaactatat agatagttta tttttacatg 6241 cgtgtatgaa cataccatta attgttcctt ctctagttga taattatcga aaaataaata 6301 tagcaaataa taaatcaaac aacgatttaa ccaaaagaga aaaagaatgt ttagcgtggg 6361 catgcgaagg aaaaagctct tgggatattt caaaaatatt aggttgcagt gagcgtactg 6421 tcactttcca tttaaccaat gcgcaaatga aactcaatac aacaaaccgc tgccaaagta 6481 tttctaaagc aattttaaca ggagcaattg attgcccata ctttaaaaat taataacact 6541 gatagtgcta gtgtagatca ctactagagc caggcatcaa ataaaacgaa aggctcagtc 6601 gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca 6661 cactggctca ccttcgggtg ggcctttctg cgtttata //