LOCUS BBa_I8004 7694 bp DNA linear 09-May-2024 DEFINITION Booperator: LuxR/RhlR/aiia, monocistronic ACCESSION BBa_I8004 VERSION BBa_I8004.1 FEATURES Location/Qualifiers misc_feature 1..20 /label=LuxR/HSL promoter 20..25 /label=-35 promoter 42..47 /label=-10 misc_feature 53..53 /label=start misc_feature 1..55 /label=BBa_R0062 promoter 1..55 /label=BBa_R0062 misc_feature 68..71 /label=conserved RBS 64..75 /label=BBa_B0034 CDS 82..843 /label=CFP (LVA) misc_feature 800..837 /label=SsrA misc_feature 838..843 /label=2 misc_feature 150..150 /label=A misc_feature 82..843 /label=BBa_E0022 misc_feature 82..843 /label=BBa_E0022 stem_loop 863..906 /label=stem_loop misc_feature 852..931 /label=BBa_B0010 terminator 852..931 /label=BBa_B0010 stem_loop 947..966 /label=T7 TE misc_feature 973..973 /label=stop polyA_site 967..980 /label=polya misc_feature 940..980 /label=BBa_B0012 terminator 940..980 /label=BBa_B0012 misc_feature 989..1008 /label=LuxR/HSL promoter 1008..1013 /label=-35 promoter 1030..1035 /label=-10 misc_feature 1041..1041 /label=start misc_feature 989..1043 /label=BBa_R0062 promoter 989..1043 /label=BBa_R0062 misc_feature 1056..1059 /label=conserved RBS 1052..1063 /label=BBa_B0034 CDS 1070..1672 /label=rhlL misc_feature 1673..1705 /label=LVA misc_feature 1712..1736 /label=Help:Barcodes CDS 1070..1711 /label=BBa_C0070 stem_loop 1756..1799 /label=stem_loop misc_feature 1745..1824 /label=BBa_B0010 terminator 1745..1824 /label=BBa_B0010 stem_loop 1840..1859 /label=T7 TE misc_feature 1866..1866 /label=stop polyA_site 1860..1873 /label=polya misc_feature 1833..1873 /label=BBa_B0012 terminator 1833..1873 /label=BBa_B0012 misc_feature 1882..1901 /label=LuxR/HSL promoter 1901..1906 /label=-35 promoter 1923..1928 /label=-10 misc_feature 1934..1934 /label=start misc_feature 1882..1936 /label=BBa_R0062 promoter 1882..1936 /label=BBa_R0062 misc_feature 1949..1952 /label=conserved RBS 1945..1956 /label=BBa_B0034 misc_feature 2542..2573 /label=LVA CDS 1963..2541 /label=luxI misc_feature 1963..2580 /label=BBa_C0061 misc_feature 2581..2605 /label=Help:Barcodes CDS 1963..2580 /label=BBa_C0061 stem_loop 2625..2668 /label=stem_loop misc_feature 2614..2693 /label=BBa_B0010 terminator 2614..2693 /label=BBa_B0010 stem_loop 2709..2728 /label=T7 TE misc_feature 2735..2735 /label=stop polyA_site 2729..2742 /label=polya misc_feature 2702..2742 /label=BBa_B0012 terminator 2702..2742 /label=BBa_B0012 misc_feature 2751..2770 /label=RhlR promoter 2769..2774 /label=-35 promoter 2792..2797 /label=-10 misc_feature 2803..2803 /label=start promoter 2751..2803 /label=BBa_R0071 misc_feature 2816..2819 /label=conserved RBS 2812..2823 /label=BBa_B0034 CDS 2830..3591 /label=YFP (LVA) misc_feature 3586..3591 /label=2 misc_feature 3547..3585 /label=SsrA misc_feature 3071..3071 /label=A (Q->L) misc_feature 2830..3591 /label=BBa_E0032 misc_feature 2830..3591 /label=BBa_E0032 stem_loop 3611..3654 /label=stem_loop misc_feature 3600..3679 /label=BBa_B0010 terminator 3600..3679 /label=BBa_B0010 stem_loop 3695..3714 /label=T7 TE misc_feature 3721..3721 /label=stop polyA_site 3715..3728 /label=polya misc_feature 3688..3728 /label=BBa_B0012 terminator 3688..3728 /label=BBa_B0012 misc_feature 3737..3756 /label=RhlR promoter 3755..3760 /label=-35 promoter 3778..3783 /label=-10 misc_feature 3789..3789 /label=start promoter 3737..3789 /label=BBa_R0071 misc_feature 3802..3805 /label=conserved RBS 3798..3809 /label=BBa_B0034 misc_feature 3816..3818 /label=start misc_feature 4599..4604 /label=2 misc_feature 4566..4598 /label=LVA CDS 3816..4565 /label=aiiA misc_feature 3816..4604 /label=BBa_C0060 misc_feature 4605..4629 /label=Help:Barcodes CDS 3816..4604 /label=BBa_C0060 stem_loop 4649..4692 /label=stem_loop misc_feature 4638..4717 /label=BBa_B0010 terminator 4638..4717 /label=BBa_B0010 stem_loop 4733..4752 /label=T7 TE misc_feature 4759..4759 /label=stop polyA_site 4753..4766 /label=polya misc_feature 4726..4766 /label=BBa_B0012 terminator 4726..4766 /label=BBa_B0012 misc_feature 4775..4793 /label=TetR 1 misc_feature 4775..4828 /label=BBa_R0040 promoter 4794..4799 /label=-35 misc_feature 4800..4818 /label=TetR 2 promoter 4817..4822 /label=-10 promoter 4775..4828 /label=BBa_R0040 misc_feature 4841..4844 /label=conserved RBS 4837..4848 /label=BBa_B0034 CDS 4858..5474 /label=tetR misc_feature 5475..5508 /label=SsrA misc_feature 5515..5539 /label=Help:Barcodes CDS 4855..5514 /label=BBa_C0040 stem_loop 5559..5602 /label=stem_loop misc_feature 5548..5627 /label=BBa_B0010 terminator 5548..5627 /label=BBa_B0010 stem_loop 5643..5662 /label=T7 TE misc_feature 5669..5669 /label=stop polyA_site 5663..5676 /label=polya misc_feature 5636..5676 /label=BBa_B0012 terminator 5636..5676 /label=BBa_B0012 misc_feature 5685..5703 /label=TetR 1 misc_feature 5685..5738 /label=BBa_R0040 promoter 5704..5709 /label=-35 misc_feature 5710..5728 /label=TetR 2 promoter 5727..5732 /label=-10 promoter 5685..5738 /label=BBa_R0040 misc_feature 5751..5754 /label=conserved RBS 5747..5758 /label=BBa_B0034 CDS 5765..6487 /label=rhlR misc_feature 6488..6520 /label=LVA misc_feature 6527..6551 /label=Help:Barcodes CDS 5765..6526 /label=BBa_C0071 stem_loop 6571..6614 /label=stem_loop misc_feature 6560..6639 /label=BBa_B0010 terminator 6560..6639 /label=BBa_B0010 stem_loop 6655..6674 /label=T7 TE misc_feature 6681..6681 /label=stop polyA_site 6675..6688 /label=polya misc_feature 6648..6688 /label=BBa_B0012 terminator 6648..6688 /label=BBa_B0012 misc_feature 6697..6715 /label=TetR 1 misc_feature 6697..6750 /label=BBa_R0040 promoter 6716..6721 /label=-35 misc_feature 6722..6740 /label=TetR 2 promoter 6739..6744 /label=-10 promoter 6697..6750 /label=BBa_R0040 misc_feature 6763..6766 /label=conserved RBS 6759..6770 /label=BBa_B0034 misc_feature 6777..6778 /label=prefix misc_feature 6950..6950 /label=T misc_feature 7268..7268 /label=A CDS 6777..7526 /label=luxR misc_feature 6777..7532 /label=BBa_C0062 misc_feature 7533..7557 /label=Help:Barcodes CDS 6777..7532 /label=BBa_C0062 stem_loop 7577..7620 /label=stem_loop misc_feature 7566..7645 /label=BBa_B0010 terminator 7566..7645 /label=BBa_B0010 stem_loop 7661..7680 /label=T7 TE misc_feature 7687..7687 /label=stop polyA_site 7681..7694 /label=polya misc_feature 7654..7694 /label=BBa_B0012 terminator 7654..7694 /label=BBa_B0012 ORIGIN 1 acctgtagga tcgtacaggt ttacgcaaga aaatggtttg ttatagtcga ataaatacta 61 gagaaagagg agaaatacta gatggtgagc aagggcgagg agctgttcac cggggtggtg 121 cccatcctgg tcgagctgga cggcgacgtg aacggccaca agttcagcgt gtccggcgag 181 ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag 241 ctgcccgtgc cctggcccac cctcgtgacc accctgacct ggggcgtgca gtgcttcagc 301 cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac 361 gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg 421 aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag 481 gacggcaaca tcctggggca caagctggag tacaactaca tcagccacaa cgtctatatc 541 accgccgaca agcagaagaa cggcatcaag gccaacttca agatccgcca caacatcgag 601 gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc 661 gtgctgctgc ccgacaacca ctacctgagc acccagtccg ccctgagcaa agaccccaac 721 gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc 781 atggacgagc tgtacaagag gcctgctgca aacgacgaaa actacgcttt agtagcttaa 841 taatactaga gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg 901 ttttatctgt tgtttgtcgg tgaacgctct ctactagagt cacactggct caccttcggg 961 tgggcctttc tgcgtttata tactagagac ctgtaggatc gtacaggttt acgcaagaaa 1021 atggtttgtt atagtcgaat aaatactaga gaaagaggag aaatactaga tgatcgaact 1081 gctgtccgaa tccctggaag gtctgtccgc tgctatgatc gctgaactgg gtcgttaccg 1141 tcaccaggtt ttcatcgaaa aactgggttg ggacgttgtt tccacctccc gtgttcgtga 1201 ccaggagttc gaccagttcg accacccgca gacccgttac atcgttgcta tgtcccgtca 1261 gggtatctgc ggttgcgctc gtctgctgcc gaccaccgac gcttacctgc tgaaagacgt 1321 tttcgcttac ctgtgctccg aaaccccgcc gtccgacccg tccgtttggg aactgtcccg 1381 ttacgctgct tccgctgctg acgacccgca gctggctatg aaaatcttct ggtcctccct 1441 ccagtgcgct tggtacctgg gtgcttcctc cgttgttgct gttaccacca ccgctatgga 1501 acgttacttc gttcgtaacg gtgttatcct ccagcgtctg ggtccgccgc agaaagttaa 1561 aggtgaaacc ctggttgcta tctccttccc ggcttaccag gaacgtggtc tggaaatgct 1621 gctgcgttac cacccggaat ggctccaggg tgttccgctg tccatggctg ttgctgcaaa 1681 cgacgaaaac tacgctttag tagcttaata actctgatag tgctagtgta gatctctact 1741 agagccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc 1801 tgttgtttgt cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct 1861 ttctgcgttt atatactaga gacctgtagg atcgtacagg tttacgcaag aaaatggttt 1921 gttatagtcg aataaatact agagaaagag gagaaatact agatgactat aatgataaaa 1981 aaatcggatt ttttggcaat tccatcggag gagtataaag gtattctaag tcttcgttat 2041 caagtgttta agcaaagact tgagtgggac ttagttgtag aaaataacct tgaatcagat 2101 gagtatgata actcaaatgc agaatatatt tatgcttgtg atgatactga aaatgtaagt 2161 ggatgctggc gtttattacc tacaacaggt gattatatgc tgaaaagtgt ttttcctgaa 2221 ttgcttggtc aacagagtgc tcccaaagat cctaatatag tcgaattaag tcgttttgct 2281 gtaggtaaaa atagctcaaa gataaataac tctgctagtg aaattacaat gaaactattt 2341 gaagctatat ataaacacgc tgttagtcaa ggtattacag aatatgtaac agtaacatca 2401 acagcaatag agcgattttt aaagcgtatt aaagttcctt gtcatcgtat tggagacaaa 2461 gaaattcatg tattaggtga tactaaatcg gttgtattgt ctatgcctat taatgaacag 2521 tttaaaaaag cagtcttaaa tgctgcaaac gacgaaaact acgctttagt agcttaataa 2581 ctctgatagt gctagtgtag atctctacta gagccaggca tcaaataaaa cgaaaggctc 2641 agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctctactaga 2701 gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta tatactagag tcctgtgaaa 2761 tctggcagtt accgttagct ttcgaattgg ctaaaaagtg ttctactaga gaaagaggag 2821 aaatactaga tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc 2881 gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat 2941 gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc 3001 tggcccaccc tcgtgaccac cttcggctac ggcctgcaat gcttcgcccg ctaccccgac 3061 cacatgaagc tgcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc 3121 accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc 3181 gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc 3241 ctggggcaca agctggagta caactacaac agccacaacg tctatatcat ggccgacaag 3301 cagaagaacg gcatcaaggt gaacttcaag atccgccaca acatcgagga cggcagcgtg 3361 cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc 3421 gacaaccact acctgagcta ccagtccgcc ctgagcaaag accccaacga gaagcgcgat 3481 cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg 3541 tacaagaggc ctgctgcaaa cgacgaaaac tacgctttag tagcttaata atactagagc 3601 caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg 3661 tttgtcggtg aacgctctct actagagtca cactggctca ccttcgggtg ggcctttctg 3721 cgtttatata ctagagtcct gtgaaatctg gcagttaccg ttagctttcg aattggctaa 3781 aaagtgttct actagagaaa gaggagaaat actagatgac agtaaagaag ctttatttcg 3841 tcccagcagg tcgttgtatg ttggatcatt cgtctgttaa tagtacatta acaccaggag 3901 aattattaga cttaccggtt tggtgttatc ttttggagac tgaagaagga cctattttag 3961 tagatacagg tatgccagaa agtgcagtta ataatgaagg tctttttaac ggtacatttg 4021 tcgaagggca ggttttaccg aaaatgactg aagaagatag aatcgtgaat attttaaaac 4081 gggttggtta tgagccggaa gaccttcttt atattattag ttctcacttg cattttgatc 4141 atgcaggagg aaatggcgct tttataaata caccaatcat tgtacagcgt gctgaatatg 4201 aggcggcgca gcatagcgaa gaatatttga aagaatgtat attgccgaat ttaaactaca 4261 aaatcattga aggtgattat gaagtcgtac caggagttca attattgcat acaccaggcc 4321 atactccagg gcatcaatcg ctattaattg agacagaaaa atccggtcct gtattattaa 4381 cgattgatgc atcgtatacg aaagagaatt ttgaaaatga agtgccattt gcgggatttg 4441 attcagaatt agctttatct tcaattaaac gtttaaaaga agtggtgatg aaagagaagc 4501 cgattgtttt ctttggacat gatatagagc aggaaagggg atgtaaagtg ttccctgaat 4561 atatagctgc aaacgacgaa aactacgctt tagtagctta ataacgctga tagtgctagt 4621 gtagatcgct actagagcca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc 4681 ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac tagagtcaca ctggctcacc 4741 ttcgggtggg cctttctgcg tttatatact agagtcccta tcagtgatag agattgacat 4801 ccctatcagt gatagagata ctgagcacta ctagagaaag aggagaaata ctagatgtcc 4861 agattagata aaagtaaagt gattaacagc gcattagagc tgcttaatga ggtcggaatc 4921 gaaggtttaa caacccgtaa actcgcccag aagctaggtg tagagcagcc tacattgtat 4981 tggcatgtaa aaaataagcg ggctttgctc gacgccttag ccattgagat gttagatagg 5041 caccatactc acttttgccc tttagaaggg gaaagctggc aagatttttt acgtaataac 5101 gctaaaagtt ttagatgtgc tttactaagt catcgcgatg gagcaaaagt acatttaggt 5161 acacggccta cagaaaaaca gtatgaaact ctcgaaaatc aattagcctt tttatgccaa 5221 caaggttttt cactagagaa tgcattatat gcactcagcg ctgtggggca ttttacttta 5281 ggttgcgtat tggaagatca agagcatcaa gtcgctaaag aagaaaggga aacacctact 5341 actgatagta tgccgccatt attacgacaa gctatcgaat tatttgatca ccaaggtgca 5401 gagccagcct tcttattcgg ccttgaattg atcatatgcg gattagaaaa acaacttaaa 5461 tgtgaaagtg ggtccgctgc aaacgacgaa aactacgctt tagtagctta ataacactga 5521 tagtgctagt gtagatcact actagagcca ggcatcaaat aaaacgaaag gctcagtcga 5581 aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac tagagtcaca 5641 ctggctcacc ttcgggtggg cctttctgcg tttatatact agagtcccta tcagtgatag 5701 agattgacat ccctatcagt gatagagata ctgagcacta ctagagaaag aggagaaata 5761 ctagatgagg aatgacggag gctttttgct gtggtgggac ggtttgcgta gcgagatgca 5821 gccgatccac gacagccagg gcgtgttcgc cgtcctggaa aaggaagtgc ggcgcctggg 5881 cttcgattac tacgcctatg gcgtgcgcca cacgattccc ttcacccggc cgaagaccga 5941 ggtccatggc acctatccca aggcctggct ggagcgatac cagatgcaga actacggggc 6001 cgtggatccg gcgatcctca acggcctgcg ctcctcggaa atggtggtct ggagcgacag 6061 cctgttcgac cagagccgga tgctctggaa cgaggctcgc gattggggcc tctgtgtcgg 6121 cgcgaccttg ccgatccgcg cgccgaacaa tttgctcagc gtgctttccg tggcgcgcga 6181 ccagcagaac atctccagct tcgagcgcga ggaaatccgc ctgcggctgc gttgcatgat 6241 cgagttgctg acccagaagc tgaccgacct ggagcatccg atgctgatgt ccaacccggt 6301 ctgcctgagc catcgcgaac gcgagatcct gcaatggacc gccgacggca agagttccgg 6361 ggaaatcgcc atcatcctga gcatctccga gagcacggtg aacttccacc acaagaacat 6421 ccagaagaag ttcgacgcgc cgaacaagac gctggctgcc gcctacgccg cggcgctggg 6481 tctcatcgct gcaaacgacg aaaactacgc tttagtagct taataacact gatagtgcta 6541 gtgtagatca ctactagagc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg 6601 gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca cactggctca 6661 ccttcgggtg ggcctttctg cgtttatata ctagagtccc tatcagtgat agagattgac 6721 atccctatca gtgatagaga tactgagcac tactagagaa agaggagaaa tactagatga 6781 aaaacataaa tgccgacgac acatacagaa taattaataa aattaaagct tgtagaagca 6841 ataatgatat taatcaatgc ttatctgata tgactaaaat ggtacattgt gaatattatt 6901 tactcgcgat catttatcct cattctatgg ttaaatctga tatttcaatc ctagataatt 6961 accctaaaaa atggaggcaa tattatgatg acgctaattt aataaaatat gatcctatag 7021 tagattattc taactccaat cattcaccaa ttaattggaa tatatttgaa aacaatgctg 7081 taaataaaaa atctccaaat gtaattaaag aagcgaaaac atcaggtctt atcactgggt 7141 ttagtttccc tattcatacg gctaacaatg gcttcggaat gcttagtttt gcacattcag 7201 aaaaagacaa ctatatagat agtttatttt tacatgcgtg tatgaacata ccattaattg 7261 ttccttctct agttgataat tatcgaaaaa taaatatagc aaataataaa tcaaacaacg 7321 atttaaccaa aagagaaaaa gaatgtttag cgtgggcatg cgaaggaaaa agctcttggg 7381 atatttcaaa aatattaggt tgcagtgagc gtactgtcac tttccattta accaatgcgc 7441 aaatgaaact caatacaaca aaccgctgcc aaagtatttc taaagcaatt ttaacaggag 7501 caattgattg cccatacttt aaaaattaat aacactgata gtgctagtgt agatcactac 7561 tagagccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat 7621 ctgttgtttg tcggtgaacg ctctctacta gagtcacact ggctcacctt cgggtgggcc 7681 tttctgcgtt tata //