LOCUS BBa_I8021 5887 bp DNA linear 26-Dec-2024 DEFINITION Booperator test: RhlR biasing, RhlR/LuxR crosstalk ACCESSION BBa_I8021 VERSION BBa_I8021.1 FEATURES Location/Qualifiers misc_feature 1..20 /label=RhlR promoter 19..24 /label=-35 promoter 42..47 /label=-10 misc_feature 53..53 /label=start promoter 1..53 /label=BBa_R0071 misc_feature 66..69 /label=conserved RBS 62..73 /label=BBa_B0034 misc_feature 818..823 /label=2 CDS 80..823 /label=cI HK022 misc_feature 785..817 /label=SsrA misc_feature 80..87 /label=OR3 partial misc_feature 80..823 /label=BBa_C0050 misc_feature 824..848 /label=Help:Barcodes CDS 80..823 /label=BBa_C0050 stem_loop 868..911 /label=stem_loop misc_feature 857..936 /label=BBa_B0010 terminator 857..936 /label=BBa_B0010 stem_loop 952..971 /label=T7 TE misc_feature 978..978 /label=stop polyA_site 972..985 /label=polya misc_feature 945..985 /label=BBa_B0012 terminator 945..985 /label=BBa_B0012 promoter 1001..1006 /label=-35 RBS 1006..1020 /label=OR2 promoter 1023..1028 /label=-10 RBS 1030..1044 /label=OR1 misc_feature 1036..1036 /label=putative misc_feature 994..1048 /label=BBa_R0050 promoter 994..1048 /label=BBa_R0050 misc_feature 1061..1064 /label=conserved RBS 1057..1068 /label=BBa_B0034 CDS 1075..1836 /label=YFP (LVA) misc_feature 1831..1836 /label=2 misc_feature 1792..1830 /label=SsrA misc_feature 1316..1316 /label=A (Q->L) misc_feature 1075..1836 /label=BBa_E0032 misc_feature 1075..1836 /label=BBa_E0032 stem_loop 1856..1899 /label=stem_loop misc_feature 1845..1924 /label=BBa_B0010 terminator 1845..1924 /label=BBa_B0010 stem_loop 1940..1959 /label=T7 TE misc_feature 1966..1966 /label=stop polyA_site 1960..1973 /label=polya misc_feature 1933..1973 /label=BBa_B0012 terminator 1933..1973 /label=BBa_B0012 misc_feature 1982..2001 /label=RhlR promoter 2000..2005 /label=-35 promoter 2023..2028 /label=-10 misc_feature 2034..2034 /label=start promoter 1982..2034 /label=BBa_R0071 misc_feature 2047..2050 /label=conserved RBS 2043..2054 /label=BBa_B0034 CDS 2061..2822 /label=CFP (LVA) misc_feature 2779..2816 /label=SsrA misc_feature 2817..2822 /label=2 misc_feature 2129..2129 /label=A misc_feature 2061..2822 /label=BBa_E0022 misc_feature 2061..2822 /label=BBa_E0022 stem_loop 2842..2885 /label=stem_loop misc_feature 2831..2910 /label=BBa_B0010 terminator 2831..2910 /label=BBa_B0010 stem_loop 2926..2945 /label=T7 TE misc_feature 2952..2952 /label=stop polyA_site 2946..2959 /label=polya misc_feature 2919..2959 /label=BBa_B0012 terminator 2919..2959 /label=BBa_B0012 misc_feature 2968..2986 /label=TetR 1 misc_feature 2968..3021 /label=BBa_R0040 promoter 2987..2992 /label=-35 misc_feature 2993..3011 /label=TetR 2 promoter 3010..3015 /label=-10 promoter 2968..3021 /label=BBa_R0040 misc_feature 3034..3037 /label=conserved RBS 3030..3041 /label=BBa_B0034 CDS 3051..3667 /label=tetR misc_feature 3668..3701 /label=SsrA misc_feature 3708..3732 /label=Help:Barcodes CDS 3048..3707 /label=BBa_C0040 stem_loop 3752..3795 /label=stem_loop misc_feature 3741..3820 /label=BBa_B0010 terminator 3741..3820 /label=BBa_B0010 stem_loop 3836..3855 /label=T7 TE misc_feature 3862..3862 /label=stop polyA_site 3856..3869 /label=polya misc_feature 3829..3869 /label=BBa_B0012 terminator 3829..3869 /label=BBa_B0012 misc_feature 3878..3896 /label=TetR 1 misc_feature 3878..3931 /label=BBa_R0040 promoter 3897..3902 /label=-35 misc_feature 3903..3921 /label=TetR 2 promoter 3920..3925 /label=-10 promoter 3878..3931 /label=BBa_R0040 misc_feature 3944..3947 /label=conserved RBS 3940..3951 /label=BBa_B0034 CDS 3958..4680 /label=rhlR misc_feature 4681..4713 /label=LVA misc_feature 4720..4744 /label=Help:Barcodes CDS 3958..4719 /label=BBa_C0071 stem_loop 4764..4807 /label=stem_loop misc_feature 4753..4832 /label=BBa_B0010 terminator 4753..4832 /label=BBa_B0010 stem_loop 4848..4867 /label=T7 TE misc_feature 4874..4874 /label=stop polyA_site 4868..4881 /label=polya misc_feature 4841..4881 /label=BBa_B0012 terminator 4841..4881 /label=BBa_B0012 misc_feature 4890..4908 /label=TetR 1 misc_feature 4890..4943 /label=BBa_R0040 promoter 4909..4914 /label=-35 misc_feature 4915..4933 /label=TetR 2 promoter 4932..4937 /label=-10 promoter 4890..4943 /label=BBa_R0040 misc_feature 4956..4959 /label=conserved RBS 4952..4963 /label=BBa_B0034 misc_feature 4970..4971 /label=prefix misc_feature 5143..5143 /label=T misc_feature 5461..5461 /label=A CDS 4970..5719 /label=luxR misc_feature 4970..5725 /label=BBa_C0062 misc_feature 5726..5750 /label=Help:Barcodes CDS 4970..5725 /label=BBa_C0062 stem_loop 5770..5813 /label=stem_loop misc_feature 5759..5838 /label=BBa_B0010 terminator 5759..5838 /label=BBa_B0010 stem_loop 5854..5873 /label=T7 TE misc_feature 5880..5880 /label=stop polyA_site 5874..5887 /label=polya misc_feature 5847..5887 /label=BBa_B0012 terminator 5847..5887 /label=BBa_B0012 ORIGIN 1 tcctgtgaaa tctggcagtt accgttagct ttcgaattgg ctaaaaagtg ttctactaga 61 gaaagaggag aaatactaga tggttcaaca gaaagagcgt gaaactttct cgcagaggct 121 tgcgctggcc tgtgataaag cgggattacc tttgcatggt aggcaggctg atttagctgt 181 caggcttaag gtcacaccaa aagccattag taaatggttc aacggggagt caataccaag 241 aaaagacaag atggaatctc tggcttcggt gctgggaact actgctgcat atctgcatgg 301 ctatgctgat gatgacggta tcacggtaaa tcatctatca agatcaaatg attattatcg 361 tgttgatgta ttggatgttc aggcgagcgc cgggccagga accatggttt ccaatgaatt 421 tatagaaaag ataagagcaa ttgaatatac gaccgagcag gcaagaattt tatttaatgg 481 aaggccacag gaaagcgtaa aagtcatcac ggttcgcggt gacagcatgg agggaaccat 541 caatccggga gatgagatct ttgttgatgt atccataacc tgttttgatg gcgatggcat 601 ttatgtgttt gtatacggga aaacaatgca cgttaagcgc ctgcaaatgc aaaagaacag 661 gcttgccgtc atctctgaca atgccgctta tgatcgatgg tacatagaag aaggtgaaga 721 agagcaactt cacattctag ccaaagtcct cattaggcag tcaatcgatt acaagcgatt 781 cggagctgca aacgacgaaa actacgcttt agtagcttaa taaccctgat agtgctagtg 841 tagatcccta ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc 901 tttcgtttta tctgttgttt gtcggtgaac gctctctact agagtcacac tggctcacct 961 tcgggtgggc ctttctgcgt ttatatacta gagttaggta ttgactgtac tatcagttcc 1021 gtcataatat gaaccataag ttcaccacta ctagagaaag aggagaaata ctagatggtg 1081 agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac 1141 gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac ctacggcaag 1201 ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg 1261 accaccttcg gctacggcct gcaatgcttc gcccgctacc ccgaccacat gaagctgcac 1321 gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat cttcttcaag 1381 gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac 1441 cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg 1501 gagtacaact acaacagcca caacgtctat atcatggccg acaagcagaa gaacggcatc 1561 aaggtgaact tcaagatccg ccacaacatc gaggacggca gcgtgcagct cgccgaccac 1621 taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg 1681 agctaccagt ccgccctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg 1741 gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa gaggcctgct 1801 gcaaacgacg aaaactacgc tttagtagct taataatact agagccaggc atcaaataaa 1861 acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc 1921 tctctactag agtcacactg gctcaccttc gggtgggcct ttctgcgttt atatactaga 1981 gtcctgtgaa atctggcagt taccgttagc tttcgaattg gctaaaaagt gttctactag 2041 agaaagagga gaaatactag atggtgagca agggcgagga gctgttcacc ggggtggtgc 2101 ccatcctggt cgagctggac ggcgacgtga acggccacaa gttcagcgtg tccggcgagg 2161 gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc 2221 tgcccgtgcc ctggcccacc ctcgtgacca ccctgacctg gggcgtgcag tgcttcagcc 2281 gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctacg 2341 tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga 2401 agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg 2461 acggcaacat cctggggcac aagctggagt acaactacat cagccacaac gtctatatca 2521 ccgccgacaa gcagaagaac ggcatcaagg ccaacttcaa gatccgccac aacatcgagg 2581 acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg 2641 tgctgctgcc cgacaaccac tacctgagca cccagtccgc cctgagcaaa gaccccaacg 2701 agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actctcggca 2761 tggacgagct gtacaagagg cctgctgcaa acgacgaaaa ctacgcttta gtagcttaat 2821 aatactagag ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt 2881 tttatctgtt gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt 2941 gggcctttct gcgtttatat actagagtcc ctatcagtga tagagattga catccctatc 3001 agtgatagag atactgagca ctactagaga aagaggagaa atactagatg tccagattag 3061 ataaaagtaa agtgattaac agcgcattag agctgcttaa tgaggtcgga atcgaaggtt 3121 taacaacccg taaactcgcc cagaagctag gtgtagagca gcctacattg tattggcatg 3181 taaaaaataa gcgggctttg ctcgacgcct tagccattga gatgttagat aggcaccata 3241 ctcacttttg ccctttagaa ggggaaagct ggcaagattt tttacgtaat aacgctaaaa 3301 gttttagatg tgctttacta agtcatcgcg atggagcaaa agtacattta ggtacacggc 3361 ctacagaaaa acagtatgaa actctcgaaa atcaattagc ctttttatgc caacaaggtt 3421 tttcactaga gaatgcatta tatgcactca gcgctgtggg gcattttact ttaggttgcg 3481 tattggaaga tcaagagcat caagtcgcta aagaagaaag ggaaacacct actactgata 3541 gtatgccgcc attattacga caagctatcg aattatttga tcaccaaggt gcagagccag 3601 ccttcttatt cggccttgaa ttgatcatat gcggattaga aaaacaactt aaatgtgaaa 3661 gtgggtccgc tgcaaacgac gaaaactacg ctttagtagc ttaataacac tgatagtgct 3721 agtgtagatc actactagag ccaggcatca aataaaacga aaggctcagt cgaaagactg 3781 ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagtc acactggctc 3841 accttcgggt gggcctttct gcgtttatat actagagtcc ctatcagtga tagagattga 3901 catccctatc agtgatagag atactgagca ctactagaga aagaggagaa atactagatg 3961 aggaatgacg gaggcttttt gctgtggtgg gacggtttgc gtagcgagat gcagccgatc 4021 cacgacagcc agggcgtgtt cgccgtcctg gaaaaggaag tgcggcgcct gggcttcgat 4081 tactacgcct atggcgtgcg ccacacgatt cccttcaccc ggccgaagac cgaggtccat 4141 ggcacctatc ccaaggcctg gctggagcga taccagatgc agaactacgg ggccgtggat 4201 ccggcgatcc tcaacggcct gcgctcctcg gaaatggtgg tctggagcga cagcctgttc 4261 gaccagagcc ggatgctctg gaacgaggct cgcgattggg gcctctgtgt cggcgcgacc 4321 ttgccgatcc gcgcgccgaa caatttgctc agcgtgcttt ccgtggcgcg cgaccagcag 4381 aacatctcca gcttcgagcg cgaggaaatc cgcctgcggc tgcgttgcat gatcgagttg 4441 ctgacccaga agctgaccga cctggagcat ccgatgctga tgtccaaccc ggtctgcctg 4501 agccatcgcg aacgcgagat cctgcaatgg accgccgacg gcaagagttc cggggaaatc 4561 gccatcatcc tgagcatctc cgagagcacg gtgaacttcc accacaagaa catccagaag 4621 aagttcgacg cgccgaacaa gacgctggct gccgcctacg ccgcggcgct gggtctcatc 4681 gctgcaaacg acgaaaacta cgctttagta gcttaataac actgatagtg ctagtgtaga 4741 tcactactag agccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc 4801 gttttatctg ttgtttgtcg gtgaacgctc tctactagag tcacactggc tcaccttcgg 4861 gtgggccttt ctgcgtttat atactagagt ccctatcagt gatagagatt gacatcccta 4921 tcagtgatag agatactgag cactactaga gaaagaggag aaatactaga tgaaaaacat 4981 aaatgccgac gacacataca gaataattaa taaaattaaa gcttgtagaa gcaataatga 5041 tattaatcaa tgcttatctg atatgactaa aatggtacat tgtgaatatt atttactcgc 5101 gatcatttat cctcattcta tggttaaatc tgatatttca atcctagata attaccctaa 5161 aaaatggagg caatattatg atgacgctaa tttaataaaa tatgatccta tagtagatta 5221 ttctaactcc aatcattcac caattaattg gaatatattt gaaaacaatg ctgtaaataa 5281 aaaatctcca aatgtaatta aagaagcgaa aacatcaggt cttatcactg ggtttagttt 5341 ccctattcat acggctaaca atggcttcgg aatgcttagt tttgcacatt cagaaaaaga 5401 caactatata gatagtttat ttttacatgc gtgtatgaac ataccattaa ttgttccttc 5461 tctagttgat aattatcgaa aaataaatat agcaaataat aaatcaaaca acgatttaac 5521 caaaagagaa aaagaatgtt tagcgtgggc atgcgaagga aaaagctctt gggatatttc 5581 aaaaatatta ggttgcagtg agcgtactgt cactttccat ttaaccaatg cgcaaatgaa 5641 actcaataca acaaaccgct gccaaagtat ttctaaagca attttaacag gagcaattga 5701 ttgcccatac tttaaaaatt aataacactg atagtgctag tgtagatcac tactagagcc 5761 aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt 5821 ttgtcggtga acgctctcta ctagagtcac actggctcac cttcgggtgg gcctttctgc 5881 gtttata //