LOCUS BBa_I8023 8596 bp DNA linear 09-May-2024 DEFINITION Booperator test: Inverter NOR biasing in polycistronic design ACCESSION BBa_I8023 VERSION BBa_I8023.1 FEATURES Location/Qualifiers misc_feature 1..20 /label=LuxR/HSL promoter 20..25 /label=-35 promoter 42..47 /label=-10 misc_feature 53..53 /label=start misc_feature 1..55 /label=BBa_R0062 promoter 1..55 /label=BBa_R0062 misc_feature 68..71 /label=conserved RBS 64..75 /label=BBa_B0034 misc_feature 820..825 /label=2 CDS 82..825 /label=cI HK022 misc_feature 787..819 /label=SsrA misc_feature 82..89 /label=OR3 partial misc_feature 82..825 /label=BBa_C0050 misc_feature 826..850 /label=Help:Barcodes CDS 82..825 /label=BBa_C0050 stem_loop 870..913 /label=stem_loop misc_feature 859..938 /label=BBa_B0010 terminator 859..938 /label=BBa_B0010 stem_loop 954..973 /label=T7 TE misc_feature 980..980 /label=stop polyA_site 974..987 /label=polya misc_feature 947..987 /label=BBa_B0012 terminator 947..987 /label=BBa_B0012 promoter 1003..1008 /label=-35 RBS 1008..1022 /label=OR2 promoter 1025..1030 /label=-10 RBS 1032..1046 /label=OR1 misc_feature 1038..1038 /label=putative misc_feature 996..1050 /label=BBa_R0050 promoter 996..1050 /label=BBa_R0050 misc_feature 1063..1066 /label=conserved RBS 1059..1070 /label=BBa_B0034 CDS 1080..1787 /label=cI lambda misc_feature 1788..1820 /label=LVA misc_feature 1827..1851 /label=Help:Barcodes CDS 1077..1826 /label=BBa_C0051 stem_loop 1871..1914 /label=stem_loop misc_feature 1860..1939 /label=BBa_B0010 terminator 1860..1939 /label=BBa_B0010 stem_loop 1955..1974 /label=T7 TE misc_feature 1981..1981 /label=stop polyA_site 1975..1988 /label=polya misc_feature 1948..1988 /label=BBa_B0012 terminator 1948..1988 /label=BBa_B0012 promoter 2034..2039 /label=-10 promoter 2011..2016 /label=-35 misc_feature 2021..2037 /label=OR1 misc_feature 1997..2013 /label=OR2 misc_feature 1997..2045 /label=BBa_R0051 promoter 1997..2045 /label=BBa_R0051 misc_feature 2058..2061 /label=conserved RBS 2054..2065 /label=BBa_B0034 CDS 2072..2833 /label=YFP (LVA) misc_feature 2828..2833 /label=2 misc_feature 2789..2827 /label=SsrA misc_feature 2313..2313 /label=A (Q->L) misc_feature 2072..2833 /label=BBa_E0032 misc_feature 2072..2833 /label=BBa_E0032 stem_loop 2853..2896 /label=stem_loop misc_feature 2842..2921 /label=BBa_B0010 terminator 2842..2921 /label=BBa_B0010 stem_loop 2937..2956 /label=T7 TE misc_feature 2963..2963 /label=stop polyA_site 2957..2970 /label=polya misc_feature 2930..2970 /label=BBa_B0012 terminator 2930..2970 /label=BBa_B0012 misc_feature 2979..2998 /label=RhlR promoter 2997..3002 /label=-35 promoter 3020..3025 /label=-10 misc_feature 3031..3031 /label=start promoter 2979..3031 /label=BBa_R0071 misc_feature 3044..3047 /label=conserved RBS 3040..3051 /label=BBa_B0034 CDS 3061..3768 /label=cI lambda misc_feature 3769..3801 /label=LVA misc_feature 3808..3832 /label=Help:Barcodes CDS 3058..3807 /label=BBa_C0051 misc_feature 3845..3848 /label=conserved RBS 3841..3852 /label=BBa_B0034 CDS 3859..4527 /label=cI 434 misc_feature 4489..4527 /label=LVA misc_feature 3859..4527 /label=BBa_C0052 misc_feature 4528..4552 /label=Help:Barcodes CDS 3859..4527 /label=BBa_C0052 stem_loop 4572..4615 /label=stem_loop misc_feature 4561..4640 /label=BBa_B0010 terminator 4561..4640 /label=BBa_B0010 stem_loop 4656..4675 /label=T7 TE misc_feature 4682..4682 /label=stop polyA_site 4676..4689 /label=polya misc_feature 4649..4689 /label=BBa_B0012 terminator 4649..4689 /label=BBa_B0012 misc_feature 4698..4740 /label=OR2 misc_feature 4705..4718 /label=OR2 misc_feature 4727..4740 /label=OR1 promoter 4698..4703 /label=-35 promoter 4721..4726 /label=-10 promoter 4699..4704 /label=-35 misc_feature 4732..4734 /label=start misc_feature 4698..4743 /label=BBa_R0052 promoter 4698..4743 /label=BBa_R0052 misc_feature 4756..4759 /label=conserved RBS 4752..4763 /label=BBa_B0034 CDS 4770..5531 /label=YFP (LVA) misc_feature 5526..5531 /label=2 misc_feature 5487..5525 /label=SsrA misc_feature 5011..5011 /label=A (Q->L) misc_feature 4770..5531 /label=BBa_E0032 misc_feature 4770..5531 /label=BBa_E0032 stem_loop 5551..5594 /label=stem_loop misc_feature 5540..5619 /label=BBa_B0010 terminator 5540..5619 /label=BBa_B0010 stem_loop 5635..5654 /label=T7 TE misc_feature 5661..5661 /label=stop polyA_site 5655..5668 /label=polya misc_feature 5628..5668 /label=BBa_B0012 terminator 5628..5668 /label=BBa_B0012 misc_feature 5677..5695 /label=TetR 1 misc_feature 5677..5730 /label=BBa_R0040 promoter 5696..5701 /label=-35 misc_feature 5702..5720 /label=TetR 2 promoter 5719..5724 /label=-10 promoter 5677..5730 /label=BBa_R0040 misc_feature 5743..5746 /label=conserved RBS 5739..5750 /label=BBa_B0034 CDS 5760..6376 /label=tetR misc_feature 6377..6410 /label=SsrA misc_feature 6417..6441 /label=Help:Barcodes CDS 5757..6416 /label=BBa_C0040 stem_loop 6461..6504 /label=stem_loop misc_feature 6450..6529 /label=BBa_B0010 terminator 6450..6529 /label=BBa_B0010 stem_loop 6545..6564 /label=T7 TE misc_feature 6571..6571 /label=stop polyA_site 6565..6578 /label=polya misc_feature 6538..6578 /label=BBa_B0012 terminator 6538..6578 /label=BBa_B0012 misc_feature 6587..6605 /label=TetR 1 misc_feature 6587..6640 /label=BBa_R0040 promoter 6606..6611 /label=-35 misc_feature 6612..6630 /label=TetR 2 promoter 6629..6634 /label=-10 promoter 6587..6640 /label=BBa_R0040 misc_feature 6653..6656 /label=conserved RBS 6649..6660 /label=BBa_B0034 CDS 6667..7389 /label=rhlR misc_feature 7390..7422 /label=LVA misc_feature 7429..7453 /label=Help:Barcodes CDS 6667..7428 /label=BBa_C0071 stem_loop 7473..7516 /label=stem_loop misc_feature 7462..7541 /label=BBa_B0010 terminator 7462..7541 /label=BBa_B0010 stem_loop 7557..7576 /label=T7 TE misc_feature 7583..7583 /label=stop polyA_site 7577..7590 /label=polya misc_feature 7550..7590 /label=BBa_B0012 terminator 7550..7590 /label=BBa_B0012 misc_feature 7599..7617 /label=TetR 1 misc_feature 7599..7652 /label=BBa_R0040 promoter 7618..7623 /label=-35 misc_feature 7624..7642 /label=TetR 2 promoter 7641..7646 /label=-10 promoter 7599..7652 /label=BBa_R0040 misc_feature 7665..7668 /label=conserved RBS 7661..7672 /label=BBa_B0034 misc_feature 7679..7680 /label=prefix misc_feature 7852..7852 /label=T misc_feature 8170..8170 /label=A CDS 7679..8428 /label=luxR misc_feature 7679..8434 /label=BBa_C0062 misc_feature 8435..8459 /label=Help:Barcodes CDS 7679..8434 /label=BBa_C0062 stem_loop 8479..8522 /label=stem_loop misc_feature 8468..8547 /label=BBa_B0010 terminator 8468..8547 /label=BBa_B0010 stem_loop 8563..8582 /label=T7 TE misc_feature 8589..8589 /label=stop polyA_site 8583..8596 /label=polya misc_feature 8556..8596 /label=BBa_B0012 terminator 8556..8596 /label=BBa_B0012 ORIGIN 1 acctgtagga tcgtacaggt ttacgcaaga aaatggtttg ttatagtcga ataaatacta 61 gagaaagagg agaaatacta gatggttcaa cagaaagagc gtgaaacttt ctcgcagagg 121 cttgcgctgg cctgtgataa agcgggatta cctttgcatg gtaggcaggc tgatttagct 181 gtcaggctta aggtcacacc aaaagccatt agtaaatggt tcaacgggga gtcaatacca 241 agaaaagaca agatggaatc tctggcttcg gtgctgggaa ctactgctgc atatctgcat 301 ggctatgctg atgatgacgg tatcacggta aatcatctat caagatcaaa tgattattat 361 cgtgttgatg tattggatgt tcaggcgagc gccgggccag gaaccatggt ttccaatgaa 421 tttatagaaa agataagagc aattgaatat acgaccgagc aggcaagaat tttatttaat 481 ggaaggccac aggaaagcgt aaaagtcatc acggttcgcg gtgacagcat ggagggaacc 541 atcaatccgg gagatgagat ctttgttgat gtatccataa cctgttttga tggcgatggc 601 atttatgtgt ttgtatacgg gaaaacaatg cacgttaagc gcctgcaaat gcaaaagaac 661 aggcttgccg tcatctctga caatgccgct tatgatcgat ggtacataga agaaggtgaa 721 gaagagcaac ttcacattct agccaaagtc ctcattaggc agtcaatcga ttacaagcga 781 ttcggagctg caaacgacga aaactacgct ttagtagctt aataaccctg atagtgctag 841 tgtagatccc tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg 901 cctttcgttt tatctgttgt ttgtcggtga acgctctcta ctagagtcac actggctcac 961 cttcgggtgg gcctttctgc gtttatatac tagagttagg tattgactgt actatcagtt 1021 ccgtcataat atgaaccata agttcaccac tactagagaa agaggagaaa tactagatga 1081 gcacaaaaaa gaaaccatta acacaagagc agcttgagga cgcacgtcgc cttaaagcaa 1141 tttatgaaaa aaagaaaaat gaacttggct tatcccagga atctgtcgca gacaagatgg 1201 ggatggggca gtcaggcgtt ggtgctttat ttaatggcat caatgcatta aatgcttata 1261 acgccgcatt gcttgcaaaa attctcaaag ttagcgttga agaatttagc ccttcaatcg 1321 ccagagaaat ctacgagatg tatgaagcgg ttagtatgca gccgtcactt agaagtgagt 1381 atgagtaccc tgttttttct catgttcagg cagggatgtt ctcacctgag cttagaacct 1441 ttaccaaagg tgatgcggag agatgggtaa gcacaaccaa aaaagccagt gattctgcat 1501 tctggcttga ggttgaaggt aattccatga ccgcaccaac aggctccaag ccaagctttc 1561 ctgacggaat gttaattctc gttgaccctg agcaggctgt tgagccaggt gatttctgca 1621 tagccagact tgggggtgat gagtttacct tcaagaaact gatcagggat agcggtcagg 1681 tgtttttaca accactaaac ccacagtacc caatgatccc atgcaatgag agttgttccg 1741 ttgtggggaa agttatcgct agtcagtggc ctgaagagac gtttggcgct gcaaacgacg 1801 aaaactacgc tttagtagct taataacgct gatagtgcta gtgtagatcg ctactagagc 1861 caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg 1921 tttgtcggtg aacgctctct actagagtca cactggctca ccttcgggtg ggcctttctg 1981 cgtttatata ctagagtaac accgtgcgtg ttgactattt tacctctggc ggtgataatg 2041 gttgctacta gagaaagagg agaaatacta gatggtgagc aagggcgagg agctgttcac 2101 cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt 2161 gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac 2221 caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accttcggct acggcctgca 2281 atgcttcgcc cgctaccccg accacatgaa gctgcacgac ttcttcaagt ccgccatgcc 2341 cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg 2401 cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga 2461 cttcaaggag gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa 2521 cgtctatatc atggccgaca agcagaagaa cggcatcaag gtgaacttca agatccgcca 2581 caacatcgag gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg 2641 cgacggcccc gtgctgctgc ccgacaacca ctacctgagc taccagtccg ccctgagcaa 2701 agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat 2761 cactctcggc atggacgagc tgtacaagag gcctgctgca aacgacgaaa actacgcttt 2821 agtagcttaa taatactaga gccaggcatc aaataaaacg aaaggctcag tcgaaagact 2881 gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct ctactagagt cacactggct 2941 caccttcggg tgggcctttc tgcgtttata tactagagtc ctgtgaaatc tggcagttac 3001 cgttagcttt cgaattggct aaaaagtgtt ctactagaga aagaggagaa atactagatg 3061 agcacaaaaa agaaaccatt aacacaagag cagcttgagg acgcacgtcg ccttaaagca 3121 atttatgaaa aaaagaaaaa tgaacttggc ttatcccagg aatctgtcgc agacaagatg 3181 gggatggggc agtcaggcgt tggtgcttta tttaatggca tcaatgcatt aaatgcttat 3241 aacgccgcat tgcttgcaaa aattctcaaa gttagcgttg aagaatttag cccttcaatc 3301 gccagagaaa tctacgagat gtatgaagcg gttagtatgc agccgtcact tagaagtgag 3361 tatgagtacc ctgttttttc tcatgttcag gcagggatgt tctcacctga gcttagaacc 3421 tttaccaaag gtgatgcgga gagatgggta agcacaacca aaaaagccag tgattctgca 3481 ttctggcttg aggttgaagg taattccatg accgcaccaa caggctccaa gccaagcttt 3541 cctgacggaa tgttaattct cgttgaccct gagcaggctg ttgagccagg tgatttctgc 3601 atagccagac ttgggggtga tgagtttacc ttcaagaaac tgatcaggga tagcggtcag 3661 gtgtttttac aaccactaaa cccacagtac ccaatgatcc catgcaatga gagttgttcc 3721 gttgtgggga aagttatcgc tagtcagtgg cctgaagaga cgtttggcgc tgcaaacgac 3781 gaaaactacg ctttagtagc ttaataacgc tgatagtgct agtgtagatc gctactagag 3841 aaagaggaga aatactagat gagtatttct tccagggtaa aaagcaaaag aatccagctt 3901 ggacttaacc aggctgaact tgctcaaaag gtggggacta cccagcagtc tatagagcag 3961 ctcgaaaacg gtaaaactaa gcgaccacgc tttttaccag aacttgcgtc agctcttggc 4021 gtaagtgttg actggctgct caatggcacc tctgattcga atgttagatt tgttgggcac 4081 gttgagccca aagggaaata tccattgatt agcatggtta gagctggttc gtggtgtgaa 4141 gcttgtgaac cctacgatat caaggacatt gatgaatggt atgacagtga cgttaactta 4201 ttaggcaatg gattctggct gaaggttgaa ggtgattcca tgacctcacc tgtaggtcaa 4261 agcatccctg aaggtcatat ggtgttagta gatactggac gggagccagt gaatggaagc 4321 cttgttgtag ccaaactgac tgacgcgaac gaagcaacat tcaagaaact ggtcatagat 4381 ggcggtcaga agtacctgaa aggcctgaat ccttcatggc ctatgactcc tatcaacgga 4441 aactgcaaga ttatcggtgt tgtcgtggaa gcgagggtaa aattcgtagc tgcaaacgac 4501 gaaaactacg ctttagtagc ttaataactc tgatagtgct agtgtagatc tctactagag 4561 ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt 4621 gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct 4681 gcgtttatat actagagttg acaaacaaga tacattgtat gaaaatacaa gaaagtttgt 4741 tgatactaga gaaagaggag aaatactaga tggtgagcaa gggcgaggag ctgttcaccg 4801 gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt 4861 ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagttc atctgcacca 4921 ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cttcggctac ggcctgcaat 4981 gcttcgcccg ctaccccgac cacatgaagc tgcacgactt cttcaagtcc gccatgcccg 5041 aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg 5101 ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact 5161 tcaaggagga cggcaacatc ctggggcaca agctggagta caactacaac agccacaacg 5221 tctatatcat ggccgacaag cagaagaacg gcatcaaggt gaacttcaag atccgccaca 5281 acatcgagga cggcagcgtg cagctcgccg accactacca gcagaacacc cccatcggcg 5341 acggccccgt gctgctgccc gacaaccact acctgagcta ccagtccgcc ctgagcaaag 5401 accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca 5461 ctctcggcat ggacgagctg tacaagaggc ctgctgcaaa cgacgaaaac tacgctttag 5521 tagcttaata atactagagc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg 5581 gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca cactggctca 5641 ccttcgggtg ggcctttctg cgtttatata ctagagtccc tatcagtgat agagattgac 5701 atccctatca gtgatagaga tactgagcac tactagagaa agaggagaaa tactagatgt 5761 ccagattaga taaaagtaaa gtgattaaca gcgcattaga gctgcttaat gaggtcggaa 5821 tcgaaggttt aacaacccgt aaactcgccc agaagctagg tgtagagcag cctacattgt 5881 attggcatgt aaaaaataag cgggctttgc tcgacgcctt agccattgag atgttagata 5941 ggcaccatac tcacttttgc cctttagaag gggaaagctg gcaagatttt ttacgtaata 6001 acgctaaaag ttttagatgt gctttactaa gtcatcgcga tggagcaaaa gtacatttag 6061 gtacacggcc tacagaaaaa cagtatgaaa ctctcgaaaa tcaattagcc tttttatgcc 6121 aacaaggttt ttcactagag aatgcattat atgcactcag cgctgtgggg cattttactt 6181 taggttgcgt attggaagat caagagcatc aagtcgctaa agaagaaagg gaaacaccta 6241 ctactgatag tatgccgcca ttattacgac aagctatcga attatttgat caccaaggtg 6301 cagagccagc cttcttattc ggccttgaat tgatcatatg cggattagaa aaacaactta 6361 aatgtgaaag tgggtccgct gcaaacgacg aaaactacgc tttagtagct taataacact 6421 gatagtgcta gtgtagatca ctactagagc caggcatcaa ataaaacgaa aggctcagtc 6481 gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca 6541 cactggctca ccttcgggtg ggcctttctg cgtttatata ctagagtccc tatcagtgat 6601 agagattgac atccctatca gtgatagaga tactgagcac tactagagaa agaggagaaa 6661 tactagatga ggaatgacgg aggctttttg ctgtggtggg acggtttgcg tagcgagatg 6721 cagccgatcc acgacagcca gggcgtgttc gccgtcctgg aaaaggaagt gcggcgcctg 6781 ggcttcgatt actacgccta tggcgtgcgc cacacgattc ccttcacccg gccgaagacc 6841 gaggtccatg gcacctatcc caaggcctgg ctggagcgat accagatgca gaactacggg 6901 gccgtggatc cggcgatcct caacggcctg cgctcctcgg aaatggtggt ctggagcgac 6961 agcctgttcg accagagccg gatgctctgg aacgaggctc gcgattgggg cctctgtgtc 7021 ggcgcgacct tgccgatccg cgcgccgaac aatttgctca gcgtgctttc cgtggcgcgc 7081 gaccagcaga acatctccag cttcgagcgc gaggaaatcc gcctgcggct gcgttgcatg 7141 atcgagttgc tgacccagaa gctgaccgac ctggagcatc cgatgctgat gtccaacccg 7201 gtctgcctga gccatcgcga acgcgagatc ctgcaatgga ccgccgacgg caagagttcc 7261 ggggaaatcg ccatcatcct gagcatctcc gagagcacgg tgaacttcca ccacaagaac 7321 atccagaaga agttcgacgc gccgaacaag acgctggctg ccgcctacgc cgcggcgctg 7381 ggtctcatcg ctgcaaacga cgaaaactac gctttagtag cttaataaca ctgatagtgc 7441 tagtgtagat cactactaga gccaggcatc aaataaaacg aaaggctcag tcgaaagact 7501 gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct ctactagagt cacactggct 7561 caccttcggg tgggcctttc tgcgtttata tactagagtc cctatcagtg atagagattg 7621 acatccctat cagtgataga gatactgagc actactagag aaagaggaga aatactagat 7681 gaaaaacata aatgccgacg acacatacag aataattaat aaaattaaag cttgtagaag 7741 caataatgat attaatcaat gcttatctga tatgactaaa atggtacatt gtgaatatta 7801 tttactcgcg atcatttatc ctcattctat ggttaaatct gatatttcaa tcctagataa 7861 ttaccctaaa aaatggaggc aatattatga tgacgctaat ttaataaaat atgatcctat 7921 agtagattat tctaactcca atcattcacc aattaattgg aatatatttg aaaacaatgc 7981 tgtaaataaa aaatctccaa atgtaattaa agaagcgaaa acatcaggtc ttatcactgg 8041 gtttagtttc cctattcata cggctaacaa tggcttcgga atgcttagtt ttgcacattc 8101 agaaaaagac aactatatag atagtttatt tttacatgcg tgtatgaaca taccattaat 8161 tgttccttct ctagttgata attatcgaaa aataaatata gcaaataata aatcaaacaa 8221 cgatttaacc aaaagagaaa aagaatgttt agcgtgggca tgcgaaggaa aaagctcttg 8281 ggatatttca aaaatattag gttgcagtga gcgtactgtc actttccatt taaccaatgc 8341 gcaaatgaaa ctcaatacaa caaaccgctg ccaaagtatt tctaaagcaa ttttaacagg 8401 agcaattgat tgcccatact ttaaaaatta ataacactga tagtgctagt gtagatcact 8461 actagagcca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt 8521 atctgttgtt tgtcggtgaa cgctctctac tagagtcaca ctggctcacc ttcgggtggg 8581 cctttctgcg tttata //