LOCUS BBa_I8026 7101 bp DNA linear 09-May-2024 DEFINITION Booperator test: LasR biasing, LasR/LuxR/RhlR crosstalk ACCESSION BBa_I8026 VERSION BBa_I8026.1 FEATURES Location/Qualifiers misc_feature 46..63 /label=OP2 misc_feature 106..125 /label=OP1 promoter 117..122 /label=-35 promoter 140..145 /label=-10 promoter 1..157 /label=BBa_R0079 misc_feature 170..173 /label=conserved RBS 166..177 /label=BBa_B0034 misc_feature 922..927 /label=2 CDS 184..927 /label=cI HK022 misc_feature 889..921 /label=SsrA misc_feature 184..191 /label=OR3 partial misc_feature 184..927 /label=BBa_C0050 misc_feature 928..952 /label=Help:Barcodes CDS 184..927 /label=BBa_C0050 stem_loop 972..1015 /label=stem_loop misc_feature 961..1040 /label=BBa_B0010 terminator 961..1040 /label=BBa_B0010 stem_loop 1056..1075 /label=T7 TE misc_feature 1082..1082 /label=stop polyA_site 1076..1089 /label=polya misc_feature 1049..1089 /label=BBa_B0012 terminator 1049..1089 /label=BBa_B0012 promoter 1105..1110 /label=-35 RBS 1110..1124 /label=OR2 promoter 1127..1132 /label=-10 RBS 1134..1148 /label=OR1 misc_feature 1140..1140 /label=putative misc_feature 1098..1152 /label=BBa_R0050 promoter 1098..1152 /label=BBa_R0050 misc_feature 1165..1168 /label=conserved RBS 1161..1172 /label=BBa_B0034 CDS 1179..1940 /label=YFP (LVA) misc_feature 1935..1940 /label=2 misc_feature 1896..1934 /label=SsrA misc_feature 1420..1420 /label=A (Q->L) misc_feature 1179..1940 /label=BBa_E0032 misc_feature 1179..1940 /label=BBa_E0032 stem_loop 1960..2003 /label=stem_loop misc_feature 1949..2028 /label=BBa_B0010 terminator 1949..2028 /label=BBa_B0010 stem_loop 2044..2063 /label=T7 TE misc_feature 2070..2070 /label=stop polyA_site 2064..2077 /label=polya misc_feature 2037..2077 /label=BBa_B0012 terminator 2037..2077 /label=BBa_B0012 misc_feature 2131..2148 /label=OP2 misc_feature 2191..2210 /label=OP1 promoter 2202..2207 /label=-35 promoter 2225..2230 /label=-10 promoter 2086..2242 /label=BBa_R0079 misc_feature 2255..2258 /label=conserved RBS 2251..2262 /label=BBa_B0034 CDS 2269..3030 /label=CFP (LVA) misc_feature 2987..3024 /label=SsrA misc_feature 3025..3030 /label=2 misc_feature 2337..2337 /label=A misc_feature 2269..3030 /label=BBa_E0022 misc_feature 2269..3030 /label=BBa_E0022 stem_loop 3050..3093 /label=stem_loop misc_feature 3039..3118 /label=BBa_B0010 terminator 3039..3118 /label=BBa_B0010 stem_loop 3134..3153 /label=T7 TE misc_feature 3160..3160 /label=stop polyA_site 3154..3167 /label=polya misc_feature 3127..3167 /label=BBa_B0012 terminator 3127..3167 /label=BBa_B0012 misc_feature 3176..3194 /label=TetR 1 misc_feature 3176..3229 /label=BBa_R0040 promoter 3195..3200 /label=-35 misc_feature 3201..3219 /label=TetR 2 promoter 3218..3223 /label=-10 promoter 3176..3229 /label=BBa_R0040 misc_feature 3242..3245 /label=conserved RBS 3238..3249 /label=BBa_B0034 CDS 3259..3875 /label=tetR misc_feature 3876..3909 /label=SsrA misc_feature 3916..3940 /label=Help:Barcodes CDS 3256..3915 /label=BBa_C0040 stem_loop 3960..4003 /label=stem_loop misc_feature 3949..4028 /label=BBa_B0010 terminator 3949..4028 /label=BBa_B0010 stem_loop 4044..4063 /label=T7 TE misc_feature 4070..4070 /label=stop polyA_site 4064..4077 /label=polya misc_feature 4037..4077 /label=BBa_B0012 terminator 4037..4077 /label=BBa_B0012 misc_feature 4086..4104 /label=TetR 1 misc_feature 4086..4139 /label=BBa_R0040 promoter 4105..4110 /label=-35 misc_feature 4111..4129 /label=TetR 2 promoter 4128..4133 /label=-10 promoter 4086..4139 /label=BBa_R0040 misc_feature 4152..4155 /label=conserved RBS 4148..4159 /label=BBa_B0034 CDS 4166..4888 /label=rhlR misc_feature 4889..4921 /label=LVA misc_feature 4928..4952 /label=Help:Barcodes CDS 4166..4927 /label=BBa_C0071 stem_loop 4972..5015 /label=stem_loop misc_feature 4961..5040 /label=BBa_B0010 terminator 4961..5040 /label=BBa_B0010 stem_loop 5056..5075 /label=T7 TE misc_feature 5082..5082 /label=stop polyA_site 5076..5089 /label=polya misc_feature 5049..5089 /label=BBa_B0012 terminator 5049..5089 /label=BBa_B0012 misc_feature 5098..5116 /label=TetR 1 misc_feature 5098..5151 /label=BBa_R0040 promoter 5117..5122 /label=-35 misc_feature 5123..5141 /label=TetR 2 promoter 5140..5145 /label=-10 promoter 5098..5151 /label=BBa_R0040 misc_feature 5164..5167 /label=conserved RBS 5160..5171 /label=BBa_B0034 misc_feature 5178..5179 /label=prefix misc_feature 5351..5351 /label=T misc_feature 5669..5669 /label=A CDS 5178..5927 /label=luxR misc_feature 5178..5933 /label=BBa_C0062 misc_feature 5934..5958 /label=Help:Barcodes CDS 5178..5933 /label=BBa_C0062 stem_loop 5978..6021 /label=stem_loop misc_feature 5967..6046 /label=BBa_B0010 terminator 5967..6046 /label=BBa_B0010 stem_loop 6062..6081 /label=T7 TE misc_feature 6088..6088 /label=stop polyA_site 6082..6095 /label=polya misc_feature 6055..6095 /label=BBa_B0012 terminator 6055..6095 /label=BBa_B0012 misc_feature 6104..6122 /label=TetR 1 misc_feature 6104..6157 /label=BBa_R0040 promoter 6123..6128 /label=-35 misc_feature 6129..6147 /label=TetR 2 promoter 6146..6151 /label=-10 promoter 6104..6157 /label=BBa_R0040 misc_feature 6170..6173 /label=conserved RBS 6166..6177 /label=BBa_B0034 CDS 6184..6900 /label=lasR misc_feature 6901..6933 /label=LVA misc_feature 6663..6663 /label=G misc_feature 6940..6964 /label=Help:Barcodes CDS 6184..6939 /label=BBa_C0079 stem_loop 6984..7027 /label=stem_loop misc_feature 6973..7052 /label=BBa_B0010 terminator 6973..7052 /label=BBa_B0010 stem_loop 7068..7087 /label=T7 TE misc_feature 7094..7094 /label=stop polyA_site 7088..7101 /label=polya misc_feature 7061..7101 /label=BBa_B0012 terminator 7061..7101 /label=BBa_B0012 ORIGIN 1 gcccctcgct gagcgcgtcc cggagctggg ggcaacctag ctgccacctg cttttctgct 61 agctattcca gcgaaaacat acagatttcc ggcgaaatca aggctacctg ccagttctgg 121 caggtttggc cgcgggttct ttttggtaca cgaaagctac tagagaaaga ggagaaatac 181 tagatggttc aacagaaaga gcgtgaaact ttctcgcaga ggcttgcgct ggcctgtgat 241 aaagcgggat tacctttgca tggtaggcag gctgatttag ctgtcaggct taaggtcaca 301 ccaaaagcca ttagtaaatg gttcaacggg gagtcaatac caagaaaaga caagatggaa 361 tctctggctt cggtgctggg aactactgct gcatatctgc atggctatgc tgatgatgac 421 ggtatcacgg taaatcatct atcaagatca aatgattatt atcgtgttga tgtattggat 481 gttcaggcga gcgccgggcc aggaaccatg gtttccaatg aatttataga aaagataaga 541 gcaattgaat atacgaccga gcaggcaaga attttattta atggaaggcc acaggaaagc 601 gtaaaagtca tcacggttcg cggtgacagc atggagggaa ccatcaatcc gggagatgag 661 atctttgttg atgtatccat aacctgtttt gatggcgatg gcatttatgt gtttgtatac 721 gggaaaacaa tgcacgttaa gcgcctgcaa atgcaaaaga acaggcttgc cgtcatctct 781 gacaatgccg cttatgatcg atggtacata gaagaaggtg aagaagagca acttcacatt 841 ctagccaaag tcctcattag gcagtcaatc gattacaagc gattcggagc tgcaaacgac 901 gaaaactacg ctttagtagc ttaataaccc tgatagtgct agtgtagatc cctactagag 961 ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt 1021 gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct 1081 gcgtttatat actagagtta ggtattgact gtactatcag ttccgtcata atatgaacca 1141 taagttcacc actactagag aaagaggaga aatactagat ggtgagcaag ggcgaggagc 1201 tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt 1261 tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagttca 1321 tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ttcggctacg 1381 gcctgcaatg cttcgcccgc taccccgacc acatgaagct gcacgacttc ttcaagtccg 1441 ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac ggcaactaca 1501 agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg 1561 gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac aactacaaca 1621 gccacaacgt ctatatcatg gccgacaagc agaagaacgg catcaaggtg aacttcaaga 1681 tccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag cagaacaccc 1741 ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagctac cagtccgccc 1801 tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg 1861 ccgggatcac tctcggcatg gacgagctgt acaagaggcc tgctgcaaac gacgaaaact 1921 acgctttagt agcttaataa tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg 1981 aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcta ctagagtcac 2041 actggctcac cttcgggtgg gcctttctgc gtttatatac tagaggcccc tcgctgagcg 2101 cgtcccggag ctgggggcaa cctagctgcc acctgctttt ctgctagcta ttccagcgaa 2161 aacatacaga tttccggcga aatcaaggct acctgccagt tctggcaggt ttggccgcgg 2221 gttctttttg gtacacgaaa gctactagag aaagaggaga aatactagat ggtgagcaag 2281 ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtgaac 2341 ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc 2401 ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc 2461 ctgacctggg gcgtgcagtg cttcagccgc taccccgacc acatgaagca gcacgacttc 2521 ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac 2581 ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc 2641 gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac 2701 aactacatca gccacaacgt ctatatcacc gccgacaagc agaagaacgg catcaaggcc 2761 aacttcaaga tccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag 2821 cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcacc 2881 cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc 2941 gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagaggcc tgctgcaaac 3001 gacgaaaact acgctttagt agcttaataa tactagagcc aggcatcaaa taaaacgaaa 3061 ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcta 3121 ctagagtcac actggctcac cttcgggtgg gcctttctgc gtttatatac tagagtccct 3181 atcagtgata gagattgaca tccctatcag tgatagagat actgagcact actagagaaa 3241 gaggagaaat actagatgtc cagattagat aaaagtaaag tgattaacag cgcattagag 3301 ctgcttaatg aggtcggaat cgaaggttta acaacccgta aactcgccca gaagctaggt 3361 gtagagcagc ctacattgta ttggcatgta aaaaataagc gggctttgct cgacgcctta 3421 gccattgaga tgttagatag gcaccatact cacttttgcc ctttagaagg ggaaagctgg 3481 caagattttt tacgtaataa cgctaaaagt tttagatgtg ctttactaag tcatcgcgat 3541 ggagcaaaag tacatttagg tacacggcct acagaaaaac agtatgaaac tctcgaaaat 3601 caattagcct ttttatgcca acaaggtttt tcactagaga atgcattata tgcactcagc 3661 gctgtggggc attttacttt aggttgcgta ttggaagatc aagagcatca agtcgctaaa 3721 gaagaaaggg aaacacctac tactgatagt atgccgccat tattacgaca agctatcgaa 3781 ttatttgatc accaaggtgc agagccagcc ttcttattcg gccttgaatt gatcatatgc 3841 ggattagaaa aacaacttaa atgtgaaagt gggtccgctg caaacgacga aaactacgct 3901 ttagtagctt aataacactg atagtgctag tgtagatcac tactagagcc aggcatcaaa 3961 taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga 4021 acgctctcta ctagagtcac actggctcac cttcgggtgg gcctttctgc gtttatatac 4081 tagagtccct atcagtgata gagattgaca tccctatcag tgatagagat actgagcact 4141 actagagaaa gaggagaaat actagatgag gaatgacgga ggctttttgc tgtggtggga 4201 cggtttgcgt agcgagatgc agccgatcca cgacagccag ggcgtgttcg ccgtcctgga 4261 aaaggaagtg cggcgcctgg gcttcgatta ctacgcctat ggcgtgcgcc acacgattcc 4321 cttcacccgg ccgaagaccg aggtccatgg cacctatccc aaggcctggc tggagcgata 4381 ccagatgcag aactacgggg ccgtggatcc ggcgatcctc aacggcctgc gctcctcgga 4441 aatggtggtc tggagcgaca gcctgttcga ccagagccgg atgctctgga acgaggctcg 4501 cgattggggc ctctgtgtcg gcgcgacctt gccgatccgc gcgccgaaca atttgctcag 4561 cgtgctttcc gtggcgcgcg accagcagaa catctccagc ttcgagcgcg aggaaatccg 4621 cctgcggctg cgttgcatga tcgagttgct gacccagaag ctgaccgacc tggagcatcc 4681 gatgctgatg tccaacccgg tctgcctgag ccatcgcgaa cgcgagatcc tgcaatggac 4741 cgccgacggc aagagttccg gggaaatcgc catcatcctg agcatctccg agagcacggt 4801 gaacttccac cacaagaaca tccagaagaa gttcgacgcg ccgaacaaga cgctggctgc 4861 cgcctacgcc gcggcgctgg gtctcatcgc tgcaaacgac gaaaactacg ctttagtagc 4921 ttaataacac tgatagtgct agtgtagatc actactagag ccaggcatca aataaaacga 4981 aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc 5041 tactagagtc acactggctc accttcgggt gggcctttct gcgtttatat actagagtcc 5101 ctatcagtga tagagattga catccctatc agtgatagag atactgagca ctactagaga 5161 aagaggagaa atactagatg aaaaacataa atgccgacga cacatacaga ataattaata 5221 aaattaaagc ttgtagaagc aataatgata ttaatcaatg cttatctgat atgactaaaa 5281 tggtacattg tgaatattat ttactcgcga tcatttatcc tcattctatg gttaaatctg 5341 atatttcaat cctagataat taccctaaaa aatggaggca atattatgat gacgctaatt 5401 taataaaata tgatcctata gtagattatt ctaactccaa tcattcacca attaattgga 5461 atatatttga aaacaatgct gtaaataaaa aatctccaaa tgtaattaaa gaagcgaaaa 5521 catcaggtct tatcactggg tttagtttcc ctattcatac ggctaacaat ggcttcggaa 5581 tgcttagttt tgcacattca gaaaaagaca actatataga tagtttattt ttacatgcgt 5641 gtatgaacat accattaatt gttccttctc tagttgataa ttatcgaaaa ataaatatag 5701 caaataataa atcaaacaac gatttaacca aaagagaaaa agaatgttta gcgtgggcat 5761 gcgaaggaaa aagctcttgg gatatttcaa aaatattagg ttgcagtgag cgtactgtca 5821 ctttccattt aaccaatgcg caaatgaaac tcaatacaac aaaccgctgc caaagtattt 5881 ctaaagcaat tttaacagga gcaattgatt gcccatactt taaaaattaa taacactgat 5941 agtgctagtg tagatcacta ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa 6001 agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact agagtcacac 6061 tggctcacct tcgggtgggc ctttctgcgt ttatatacta gagtccctat cagtgataga 6121 gattgacatc cctatcagtg atagagatac tgagcactac tagagaaaga ggagaaatac 6181 tagatggcct tggttgacgg ttttcttgag ctggaacgct caagtggaaa attggagtgg 6241 agcgccatcc tccagaagat ggcgagcgac cttggattct cgaagatcct gttcggcctg 6301 ttgcctaagg acagccagga ctacgagaac gccttcatcg tcggcaacta cccggccgcc 6361 tggcgcgagc attacgaccg ggctggctac gcgcgggtcg acccgacggt cagtcactgt 6421 acccagagcg tactgccgat tttctgggaa ccgtccatct accagacgcg aaagcagcac 6481 gagttcttcg aggaagcctc ggccgccggc ctggtgtatg ggctgaccat gccgctgcat 6541 ggtgctcgcg gcgaactcgg cgcgctgagc ctcagcgtgg aagcggaaaa ccgggccgag 6601 gccaaccgtt tcatagagtc ggtcctgccg accctgtgga tgctcaagga ctacgcactg 6661 caaagcggtg ccggactggc cttcgaacat ccggtcagca aaccggtggt tctgaccagc 6721 cgggagaagg aagtgttgca gtggtgcgcc atcggcaaga ccagttggga gatatcggtt 6781 atctgcaact gctcggaagc caatgtgaac ttccatatgg gaaatattcg gcggaagttc 6841 ggtgtgacct cccgccgcgt agcggccatt atggccgtta atttgggtct tattactctc 6901 gctgcaaacg acgaaaacta cgctttagta gcttaataac actgatagtg ctagtgtaga 6961 tcactactag agccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc 7021 gttttatctg ttgtttgtcg gtgaacgctc tctactagag tcacactggc tcaccttcgg 7081 gtgggccttt ctgcgtttat a //