LOCUS BBa_I8030 3541 bp DNA linear 26-Dec-2024 DEFINITION Booperator aiia test: degradation of HSL "B" by aiia ACCESSION BBa_I8030 VERSION BBa_I8030.1 FEATURES Location/Qualifiers misc_feature 1..19 /label=TetR 1 misc_feature 1..54 /label=BBa_R0040 promoter 20..25 /label=-35 misc_feature 26..44 /label=TetR 2 promoter 43..48 /label=-10 promoter 1..54 /label=BBa_R0040 misc_feature 67..70 /label=conserved RBS 63..74 /label=BBa_B0034 CDS 84..700 /label=tetR misc_feature 701..734 /label=SsrA misc_feature 741..765 /label=Help:Barcodes CDS 81..740 /label=BBa_C0040 misc_feature 778..781 /label=conserved RBS 774..785 /label=BBa_B0034 CDS 792..1514 /label=rhlR misc_feature 1515..1547 /label=LVA misc_feature 1554..1578 /label=Help:Barcodes CDS 792..1553 /label=BBa_C0071 misc_feature 1591..1594 /label=conserved RBS 1587..1598 /label=BBa_B0034 misc_feature 1605..1607 /label=start misc_feature 2388..2393 /label=2 misc_feature 2355..2387 /label=LVA CDS 1605..2354 /label=aiiA misc_feature 1605..2393 /label=BBa_C0060 misc_feature 2394..2418 /label=Help:Barcodes CDS 1605..2393 /label=BBa_C0060 stem_loop 2438..2481 /label=stem_loop misc_feature 2427..2506 /label=BBa_B0010 terminator 2427..2506 /label=BBa_B0010 stem_loop 2522..2541 /label=T7 TE misc_feature 2548..2548 /label=stop polyA_site 2542..2555 /label=polya misc_feature 2515..2555 /label=BBa_B0012 terminator 2515..2555 /label=BBa_B0012 misc_feature 2564..2583 /label=RhlR promoter 2582..2587 /label=-35 promoter 2605..2610 /label=-10 misc_feature 2616..2616 /label=start promoter 2564..2616 /label=BBa_R0071 misc_feature 2629..2632 /label=conserved RBS 2625..2636 /label=BBa_B0034 CDS 2643..3404 /label=YFP (LVA) misc_feature 3399..3404 /label=2 misc_feature 3360..3398 /label=SsrA misc_feature 2884..2884 /label=A (Q->L) misc_feature 2643..3404 /label=BBa_E0032 misc_feature 2643..3404 /label=BBa_E0032 stem_loop 3424..3467 /label=stem_loop misc_feature 3413..3492 /label=BBa_B0010 terminator 3413..3492 /label=BBa_B0010 stem_loop 3508..3527 /label=T7 TE misc_feature 3534..3534 /label=stop polyA_site 3528..3541 /label=polya misc_feature 3501..3541 /label=BBa_B0012 terminator 3501..3541 /label=BBa_B0012 ORIGIN 1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag 61 agaaagagga gaaatactag atgtccagat tagataaaag taaagtgatt aacagcgcat 121 tagagctgct taatgaggtc ggaatcgaag gtttaacaac ccgtaaactc gcccagaagc 181 taggtgtaga gcagcctaca ttgtattggc atgtaaaaaa taagcgggct ttgctcgacg 241 ccttagccat tgagatgtta gataggcacc atactcactt ttgcccttta gaaggggaaa 301 gctggcaaga ttttttacgt aataacgcta aaagttttag atgtgcttta ctaagtcatc 361 gcgatggagc aaaagtacat ttaggtacac ggcctacaga aaaacagtat gaaactctcg 421 aaaatcaatt agccttttta tgccaacaag gtttttcact agagaatgca ttatatgcac 481 tcagcgctgt ggggcatttt actttaggtt gcgtattgga agatcaagag catcaagtcg 541 ctaaagaaga aagggaaaca cctactactg atagtatgcc gccattatta cgacaagcta 601 tcgaattatt tgatcaccaa ggtgcagagc cagccttctt attcggcctt gaattgatca 661 tatgcggatt agaaaaacaa cttaaatgtg aaagtgggtc cgctgcaaac gacgaaaact 721 acgctttagt agcttaataa cactgatagt gctagtgtag atcactacta gagaaagagg 781 agaaatacta gatgaggaat gacggaggct ttttgctgtg gtgggacggt ttgcgtagcg 841 agatgcagcc gatccacgac agccagggcg tgttcgccgt cctggaaaag gaagtgcggc 901 gcctgggctt cgattactac gcctatggcg tgcgccacac gattcccttc acccggccga 961 agaccgaggt ccatggcacc tatcccaagg cctggctgga gcgataccag atgcagaact 1021 acggggccgt ggatccggcg atcctcaacg gcctgcgctc ctcggaaatg gtggtctgga 1081 gcgacagcct gttcgaccag agccggatgc tctggaacga ggctcgcgat tggggcctct 1141 gtgtcggcgc gaccttgccg atccgcgcgc cgaacaattt gctcagcgtg ctttccgtgg 1201 cgcgcgacca gcagaacatc tccagcttcg agcgcgagga aatccgcctg cggctgcgtt 1261 gcatgatcga gttgctgacc cagaagctga ccgacctgga gcatccgatg ctgatgtcca 1321 acccggtctg cctgagccat cgcgaacgcg agatcctgca atggaccgcc gacggcaaga 1381 gttccgggga aatcgccatc atcctgagca tctccgagag cacggtgaac ttccaccaca 1441 agaacatcca gaagaagttc gacgcgccga acaagacgct ggctgccgcc tacgccgcgg 1501 cgctgggtct catcgctgca aacgacgaaa actacgcttt agtagcttaa taacactgat 1561 agtgctagtg tagatcacta ctagagaaag aggagaaata ctagatgaca gtaaagaagc 1621 tttatttcgt cccagcaggt cgttgtatgt tggatcattc gtctgttaat agtacattaa 1681 caccaggaga attattagac ttaccggttt ggtgttatct tttggagact gaagaaggac 1741 ctattttagt agatacaggt atgccagaaa gtgcagttaa taatgaaggt ctttttaacg 1801 gtacatttgt cgaagggcag gttttaccga aaatgactga agaagataga atcgtgaata 1861 ttttaaaacg ggttggttat gagccggaag accttcttta tattattagt tctcacttgc 1921 attttgatca tgcaggagga aatggcgctt ttataaatac accaatcatt gtacagcgtg 1981 ctgaatatga ggcggcgcag catagcgaag aatatttgaa agaatgtata ttgccgaatt 2041 taaactacaa aatcattgaa ggtgattatg aagtcgtacc aggagttcaa ttattgcata 2101 caccaggcca tactccaggg catcaatcgc tattaattga gacagaaaaa tccggtcctg 2161 tattattaac gattgatgca tcgtatacga aagagaattt tgaaaatgaa gtgccatttg 2221 cgggatttga ttcagaatta gctttatctt caattaaacg tttaaaagaa gtggtgatga 2281 aagagaagcc gattgttttc tttggacatg atatagagca ggaaagggga tgtaaagtgt 2341 tccctgaata tatagctgca aacgacgaaa actacgcttt agtagcttaa taacgctgat 2401 agtgctagtg tagatcgcta ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa 2461 agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact agagtcacac 2521 tggctcacct tcgggtgggc ctttctgcgt ttatatacta gagtcctgtg aaatctggca 2581 gttaccgtta gctttcgaat tggctaaaaa gtgttctact agagaaagag gagaaatact 2641 agatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg 2701 acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct 2761 acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca 2821 ccctcgtgac caccttcggc tacggcctgc aatgcttcgc ccgctacccc gaccacatga 2881 agctgcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct 2941 tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc 3001 tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc 3061 acaagctgga gtacaactac aacagccaca acgtctatat catggccgac aagcagaaga 3121 acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc gtgcagctcg 3181 ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc 3241 actacctgag ctaccagtcc gccctgagca aagaccccaa cgagaagcgc gatcacatgg 3301 tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtacaaga 3361 ggcctgctgc aaacgacgaa aactacgctt tagtagctta ataatactag agccaggcat 3421 caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg 3481 gtgaacgctc tctactagag tcacactggc tcaccttcgg gtgggccttt ctgcgtttat 3541 a //