LOCUS BBa_I8031 3537 bp DNA linear 09-May-2024 DEFINITION Booperator aiia test: degradation of HSL "A" by aiia ACCESSION BBa_I8031 VERSION BBa_I8031.1 FEATURES Location/Qualifiers misc_feature 1..19 /label=TetR 1 misc_feature 1..54 /label=BBa_R0040 promoter 20..25 /label=-35 misc_feature 26..44 /label=TetR 2 promoter 43..48 /label=-10 promoter 1..54 /label=BBa_R0040 misc_feature 67..70 /label=conserved RBS 63..74 /label=BBa_B0034 CDS 84..700 /label=tetR misc_feature 701..734 /label=SsrA misc_feature 741..765 /label=Help:Barcodes CDS 81..740 /label=BBa_C0040 misc_feature 778..781 /label=conserved RBS 774..785 /label=BBa_B0034 misc_feature 792..793 /label=prefix misc_feature 965..965 /label=T misc_feature 1283..1283 /label=A CDS 792..1541 /label=luxR misc_feature 792..1547 /label=BBa_C0062 misc_feature 1548..1572 /label=Help:Barcodes CDS 792..1547 /label=BBa_C0062 misc_feature 1585..1588 /label=conserved RBS 1581..1592 /label=BBa_B0034 misc_feature 1599..1601 /label=start misc_feature 2382..2387 /label=2 misc_feature 2349..2381 /label=LVA CDS 1599..2348 /label=aiiA misc_feature 1599..2387 /label=BBa_C0060 misc_feature 2388..2412 /label=Help:Barcodes CDS 1599..2387 /label=BBa_C0060 stem_loop 2432..2475 /label=stem_loop misc_feature 2421..2500 /label=BBa_B0010 terminator 2421..2500 /label=BBa_B0010 stem_loop 2516..2535 /label=T7 TE misc_feature 2542..2542 /label=stop polyA_site 2536..2549 /label=polya misc_feature 2509..2549 /label=BBa_B0012 terminator 2509..2549 /label=BBa_B0012 misc_feature 2558..2577 /label=LuxR/HSL promoter 2577..2582 /label=-35 promoter 2599..2604 /label=-10 misc_feature 2610..2610 /label=start misc_feature 2558..2612 /label=BBa_R0062 promoter 2558..2612 /label=BBa_R0062 misc_feature 2625..2628 /label=conserved RBS 2621..2632 /label=BBa_B0034 CDS 2639..3400 /label=YFP (LVA) misc_feature 3395..3400 /label=2 misc_feature 3356..3394 /label=SsrA misc_feature 2880..2880 /label=A (Q->L) misc_feature 2639..3400 /label=BBa_E0032 misc_feature 2639..3400 /label=BBa_E0032 stem_loop 3420..3463 /label=stem_loop misc_feature 3409..3488 /label=BBa_B0010 terminator 3409..3488 /label=BBa_B0010 stem_loop 3504..3523 /label=T7 TE misc_feature 3530..3530 /label=stop polyA_site 3524..3537 /label=polya misc_feature 3497..3537 /label=BBa_B0012 terminator 3497..3537 /label=BBa_B0012 ORIGIN 1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag 61 agaaagagga gaaatactag atgtccagat tagataaaag taaagtgatt aacagcgcat 121 tagagctgct taatgaggtc ggaatcgaag gtttaacaac ccgtaaactc gcccagaagc 181 taggtgtaga gcagcctaca ttgtattggc atgtaaaaaa taagcgggct ttgctcgacg 241 ccttagccat tgagatgtta gataggcacc atactcactt ttgcccttta gaaggggaaa 301 gctggcaaga ttttttacgt aataacgcta aaagttttag atgtgcttta ctaagtcatc 361 gcgatggagc aaaagtacat ttaggtacac ggcctacaga aaaacagtat gaaactctcg 421 aaaatcaatt agccttttta tgccaacaag gtttttcact agagaatgca ttatatgcac 481 tcagcgctgt ggggcatttt actttaggtt gcgtattgga agatcaagag catcaagtcg 541 ctaaagaaga aagggaaaca cctactactg atagtatgcc gccattatta cgacaagcta 601 tcgaattatt tgatcaccaa ggtgcagagc cagccttctt attcggcctt gaattgatca 661 tatgcggatt agaaaaacaa cttaaatgtg aaagtgggtc cgctgcaaac gacgaaaact 721 acgctttagt agcttaataa cactgatagt gctagtgtag atcactacta gagaaagagg 781 agaaatacta gatgaaaaac ataaatgccg acgacacata cagaataatt aataaaatta 841 aagcttgtag aagcaataat gatattaatc aatgcttatc tgatatgact aaaatggtac 901 attgtgaata ttatttactc gcgatcattt atcctcattc tatggttaaa tctgatattt 961 caatcctaga taattaccct aaaaaatgga ggcaatatta tgatgacgct aatttaataa 1021 aatatgatcc tatagtagat tattctaact ccaatcattc accaattaat tggaatatat 1081 ttgaaaacaa tgctgtaaat aaaaaatctc caaatgtaat taaagaagcg aaaacatcag 1141 gtcttatcac tgggtttagt ttccctattc atacggctaa caatggcttc ggaatgctta 1201 gttttgcaca ttcagaaaaa gacaactata tagatagttt atttttacat gcgtgtatga 1261 acataccatt aattgttcct tctctagttg ataattatcg aaaaataaat atagcaaata 1321 ataaatcaaa caacgattta accaaaagag aaaaagaatg tttagcgtgg gcatgcgaag 1381 gaaaaagctc ttgggatatt tcaaaaatat taggttgcag tgagcgtact gtcactttcc 1441 atttaaccaa tgcgcaaatg aaactcaata caacaaaccg ctgccaaagt atttctaaag 1501 caattttaac aggagcaatt gattgcccat actttaaaaa ttaataacac tgatagtgct 1561 agtgtagatc actactagag aaagaggaga aatactagat gacagtaaag aagctttatt 1621 tcgtcccagc aggtcgttgt atgttggatc attcgtctgt taatagtaca ttaacaccag 1681 gagaattatt agacttaccg gtttggtgtt atcttttgga gactgaagaa ggacctattt 1741 tagtagatac aggtatgcca gaaagtgcag ttaataatga aggtcttttt aacggtacat 1801 ttgtcgaagg gcaggtttta ccgaaaatga ctgaagaaga tagaatcgtg aatattttaa 1861 aacgggttgg ttatgagccg gaagaccttc tttatattat tagttctcac ttgcattttg 1921 atcatgcagg aggaaatggc gcttttataa atacaccaat cattgtacag cgtgctgaat 1981 atgaggcggc gcagcatagc gaagaatatt tgaaagaatg tatattgccg aatttaaact 2041 acaaaatcat tgaaggtgat tatgaagtcg taccaggagt tcaattattg catacaccag 2101 gccatactcc agggcatcaa tcgctattaa ttgagacaga aaaatccggt cctgtattat 2161 taacgattga tgcatcgtat acgaaagaga attttgaaaa tgaagtgcca tttgcgggat 2221 ttgattcaga attagcttta tcttcaatta aacgtttaaa agaagtggtg atgaaagaga 2281 agccgattgt tttctttgga catgatatag agcaggaaag gggatgtaaa gtgttccctg 2341 aatatatagc tgcaaacgac gaaaactacg ctttagtagc ttaataacgc tgatagtgct 2401 agtgtagatc gctactagag ccaggcatca aataaaacga aaggctcagt cgaaagactg 2461 ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagtc acactggctc 2521 accttcgggt gggcctttct gcgtttatat actagagacc tgtaggatcg tacaggttta 2581 cgcaagaaaa tggtttgtta tagtcgaata aatactagag aaagaggaga aatactagat 2641 ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg 2701 cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg 2761 caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct 2821 cgtgaccacc ttcggctacg gcctgcaatg cttcgcccgc taccccgacc acatgaagct 2881 gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt 2941 caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt 3001 gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa 3061 gctggagtac aactacaaca gccacaacgt ctatatcatg gccgacaagc agaagaacgg 3121 catcaaggtg aacttcaaga tccgccacaa catcgaggac ggcagcgtgc agctcgccga 3181 ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta 3241 cctgagctac cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct 3301 gctggagttc gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagaggcc 3361 tgctgcaaac gacgaaaact acgctttagt agcttaataa tactagagcc aggcatcaaa 3421 taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga 3481 acgctctcta ctagagtcac actggctcac cttcgggtgg gcctttctgc gtttata //